Framework Nucleic Acid-Nanobody Fusion Probe-Based Pharmacokinetics Modulation and Analysis for Efficient Positron Emission Tomography Imaging DOI
Yani Yang,

Zhiqiang Ren,

Dan Wang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Nanobodies are promising for immunoPET imaging due to their excellent antigen recognition and tumor targeting, yet rapid clearance limits accumulation. Although multimerization albumin binding can extend circulation time improve a simple universal method creating protein multimers is still needed. Here, we leveraged the facile synthesis, controllable size, precise assembly of DNA nanotechnology construct CD47-targeted framework nucleic acid-nanobody fusion probes with multiple valences sizes. Following comprehensive structural characterization, in vitro specificity assessment vivo PET/CT analysis were conducted on colorectal cancer LS174T mouse model. Furthermore, pharmacokinetic model was developed fitted considerable data prove its rationality, followed by testing effects uptake prediction changing different parameters. Indeed, manipulating size acid scaffolding number attached nanobodies, could precisely modulate accumulation at site. Overall, this study not only an efficient strategy constructing nanobody but also provided model, allowing profound insight into multidimensional obtained experimentally informing design future predictable delivery efficacies.

Language: Английский

Surface Functionalization of Nanocarriers with Anti-EGFR Ligands for Cancer Active Targeting DOI Creative Commons

Alessandra Spada,

Sandrine Gerber‐Lemaire

Nanomaterials, Journal Year: 2025, Volume and Issue: 15(3), P. 158 - 158

Published: Jan. 21, 2025

Active cancer targeting consists of the selective recognition overexpressed biomarkers on cell surfaces or within tumor microenvironment, enabled by ligands conjugated to drug carriers. Nanoparticle (NP)-based systems are highly relevant for such an approach due their large surface area which is amenable a variety chemical modifications. Over past decades, several studies have debated efficiency passive targeting, highlighting active as more specific and approach. The choice conjugation chemistry attaching nanocarriers critical ensure stable robust system. Among panel biomarkers, epidermal growth factor receptor (EGFR) stands one most frequently receptors in different types. design development with surface-bound anti-EGFR vital targeted therapy, relying facilitated capture EGFR-overexpressing cells enabling receptor-mediated endocytosis improve accumulation microenvironment. In this review, we examine examples recent significant explore various strategies NP functionalization biomolecules small molecular ligands. addition, also describe some common characterization techniques confirm analyze patterns.

Language: Английский

Citations

3
Trends in nanobody radiotheranostics DOI
Xingbo Long,

Sixuan Cheng,

Xiaoli Lan

et al.

European Journal of Nuclear Medicine and Molecular Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Language: Английский

Citations

2
Development and evaluation of Hsp90-targeting nanobodies for visualisation of extracellular Hsp90 in tumours using PET imaging DOI Creative Commons

Valeria Narykina,

Janke Kleynhans, Christopher Cawthorne

et al.

EJNMMI Radiopharmacy and Chemistry, Journal Year: 2025, Volume and Issue: 10(1)

Published: Feb. 21, 2025

Language: Английский

Citations

1
Nanoscale warriors against viral invaders: a comprehensive review of Nanobodies as potential antiviral therapeutics DOI Creative Commons
Vaishali Verma,

N. D. Sinha,

A. Raja

et al.

mAbs, Journal Year: 2025, Volume and Issue: 17(1)

Published: April 9, 2025

Viral infections remain a significant global health threat, with emerging and reemerging viruses causing epidemics pandemics. Despite advancements in antiviral therapies, the development of effective treatments is often hindered by challenges, such as viral resistance emergence new strains. In this context, novel therapeutic modalities essential to combat notorious viruses. While traditional monoclonal antibodies are widely used for treatment several diseases, nanobodies derived from heavy chain-only have emerged promising "nanoscale warriors" against infections. Nanobodies possess unique structural properties that enhance their ability recognize diverse epitopes. Their small size also imparts properties, improved bioavailability, solubility, stability, proteolytic resistance, making them an ideal class therapeutics review, we discuss role antivirals various Techniques developing nanobodies, delivery strategies covered, challenges opportunities associated use therapies discussed. We offer insights into future nanobody-based research support managing

Language: Английский

Citations

1
Development and evaluation of albumin binder-conjugated heterodimeric radiopharmaceuticals targeting integrin αvβ3 and CD13 for cancer therapy DOI

Biao Yang,

Changyu Shan,

Xiangming Song

et al.

European Journal of Nuclear Medicine and Molecular Imaging, Journal Year: 2024, Volume and Issue: 51(11), P. 3334 - 3345

Published: May 24, 2024

Language: Английский

Citations

5
Metal-based nanomedicine systems for the diagnosis and treatment of colorectal cancer: Current advances and future perspectives DOI

Jie Gou,

Lu Chen,

Tianyu Liu

et al.

Materials Today Chemistry, Journal Year: 2025, Volume and Issue: 45, P. 102702 - 102702

Published: April 1, 2025

Language: Английский

Citations

0
Framework Nucleic Acid-Nanobody Fusion Probe-Based Pharmacokinetics Modulation and Analysis for Efficient Positron Emission Tomography Imaging DOI
Yani Yang,

Zhiqiang Ren,

Dan Wang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Nanobodies are promising for immunoPET imaging due to their excellent antigen recognition and tumor targeting, yet rapid clearance limits accumulation. Although multimerization albumin binding can extend circulation time improve a simple universal method creating protein multimers is still needed. Here, we leveraged the facile synthesis, controllable size, precise assembly of DNA nanotechnology construct CD47-targeted framework nucleic acid-nanobody fusion probes with multiple valences sizes. Following comprehensive structural characterization, in vitro specificity assessment vivo PET/CT analysis were conducted on colorectal cancer LS174T mouse model. Furthermore, pharmacokinetic model was developed fitted considerable data prove its rationality, followed by testing effects uptake prediction changing different parameters. Indeed, manipulating size acid scaffolding number attached nanobodies, could precisely modulate accumulation at site. Overall, this study not only an efficient strategy constructing nanobody but also provided model, allowing profound insight into multidimensional obtained experimentally informing design future predictable delivery efficacies.

Language: Английский

Citations

1