The Yeast Gsk-3 Kinase Mck1 Is Necessary for Cell Wall Remodeling in Glucose-Starved and Cell Wall-Stressed Cells

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3534 - 3534

Published: April 9, 2025

The cell wall integrity (CWI) pathway is responsible for transcriptional regulation of remodeling in response to stress. How mediated by the CWI effected inputs from other signaling pathways not well understood. Here, we demonstrate that Mck1 kinase cooperates with Slt2, MAP pathway, promote thickening glucose-starved cells. Integrative analyses transcriptome, proteome and metabolic profiling indicate required accumulation UDP-glucose (UDPG), substrate β-glucan synthesis, through activation two regulons: Msn2/4-dependent stress Cat8-/Adr1-mediated reprogram dependent on SNF1 complex. Analysis phosphoproteome suggests similar mammalian Gsk-3 kinases, involved cytoskeleton-dependent cellular processes, metabolism, transcription. Specifically, may be implicated Snf1-dependent PKA inhibition SAGA (Spt-Ada-Gcn5 acetyltransferase)-mediated transcription activation, a hypothesis further underscored significant overlap between Mck1- Gcn5-activated transcriptomes. Phenotypic analysis also supports roles actin cytoskeleton-mediated exocytosis ensure plasma membrane homeostasis wall-stressed Together, these findings only reveal novel functions reprogramming polarized growth but provide valuable omics resources future studies uncover underlying mechanisms kinases response.

Language: Английский

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Physiology and Robustness of Yeasts Exposed to Dynamic pH and Glucose Environments

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Gradients negatively affect performance in large-scale bioreactors; however, they are difficult to predict at laboratory scale. Dynamic microfluidics single-cell cultivation (dMSCC) has emerged as an important tool for investigating cell behavior rapidly changing environments. In the present study, dMSCC, biosensors of intracellular parameters, and robustness quantification were employed investigate physiological response three Saccharomyces cerevisiae strains substrate pH changes every 0.75-48 min. All showed higher sensitivity than oscillations. Strain-specific responses included relative glycolytic flux oxidative stress PE2 CEN.PK113-7D, respectively. Instead, Ethanol Red strain displayed least heterogeneous populations highest multiple functions when exposed This result could arise from a positive trade-off between ATP levels stability over time. The study demonstrates importance coupling dynamic environments with simultaneous characterization strains, conditions, individual regimes, analysis. these tools suitable add-on traditional evaluation screening workflows both industrial scale, can help bridge gap two.

Language: Английский

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Role of Adenosine Monophosphate Deaminase during Fatty Acid Accumulation in Oleaginous Fungus Mortierella alpina

Journal of Agricultural and Food Chemistry, Journal Year: 2019, Volume and Issue: 67(34), P. 9551 - 9559

Published: Aug. 5, 2019

In oleaginous micro-organisms, nitrogen limitation activates adenosine monophosphate deaminase (AMPD) and promotes lipogenesis via the inhibition of isocitrate dehydrogenase. We found that overexpression homologous AMPD in Mortierella alpina favored lipid synthesis over cell growth. Total fatty acid content recombinant strain was 15.0–34.3% higher than control, even though their biomass similar. During early fermentation stage, intracellular AMP level reduced by 40–60%, together with a 1.9–2.7-fold increase citrate compared therefore provided more precursors for synthesis. Moreover, decreased resulted metabolic reprogramming, reflected blocked TCA cycle reduction amino acids, distributing carbon to pathways. By coupling energy balance lipogenesis, this study provides new insights into metabolism under nitrogen-limited conditions targets regulation accumulation micro-organisms.

Language: Английский

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Stress and ageing in yeast

FEMS Yeast Research, Journal Year: 2019, Volume and Issue: 20(1)

Published: Dec. 6, 2019

There has long been speculation about the role of various stresses in ageing. Some have beneficial effects on ageing-dependent duration and severity stress, others negative question arises whether these are causative ageing or result process. Cellular responses to many highly coordinated a concerted way hence there is great deal cross-talk between different stresses. Here relevant aspects coordination stress roles yeast cell discussed, together with functions that involved. The cellular processes involved alleviating considered, possible early events subsequent cells.

Language: Английский

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The Yeast Protein Kinase Sch9 Functions as a Central Nutrient-Responsive Hub That Calibrates Metabolic and Stress-Related Responses

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(8), P. 787 - 787

Published: July 26, 2023

Yeast cells are equipped with different nutrient signaling pathways that enable them to sense the availability of various nutrients and adjust metabolism growth accordingly. These part an intricate network since most cross-regulated subject feedback regulation at levels. In yeast, a central role is played by Sch9, protein kinase functions as proximal effector conserved growth-regulatory TORC1 complex mediate information on free amino acids. However, recent studies established Sch9 more than TORC1-effector its activity tuned several other kinases. This allows function integrator aligns input signals achieve accuracy in metabolic responses stress-related molecular adaptations. this review, we highlight latest findings structure well nutrient-responsive hub impacts longevity yeast cells. Given key players impinging well-conserved, also discuss how can be instrumental further elucidate mechanisms underpinning healthy aging mammalians.

Language: Английский

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