Journal of Fungi,
Journal Year:
2023,
Volume and Issue:
9(8), P. 787 - 787
Published: July 26, 2023
Yeast
cells
are
equipped
with
different
nutrient
signaling
pathways
that
enable
them
to
sense
the
availability
of
various
nutrients
and
adjust
metabolism
growth
accordingly.
These
part
an
intricate
network
since
most
cross-regulated
subject
feedback
regulation
at
levels.
In
yeast,
a
central
role
is
played
by
Sch9,
protein
kinase
functions
as
proximal
effector
conserved
growth-regulatory
TORC1
complex
mediate
information
on
free
amino
acids.
However,
recent
studies
established
Sch9
more
than
TORC1-effector
its
activity
tuned
several
other
kinases.
This
allows
function
integrator
aligns
input
signals
achieve
accuracy
in
metabolic
responses
stress-related
molecular
adaptations.
this
review,
we
highlight
latest
findings
structure
well
nutrient-responsive
hub
impacts
longevity
yeast
cells.
Given
key
players
impinging
well-conserved,
also
discuss
how
can
be
instrumental
further
elucidate
mechanisms
underpinning
healthy
aging
mammalians.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(8), P. 3534 - 3534
Published: April 9, 2025
The
cell
wall
integrity
(CWI)
pathway
is
responsible
for
transcriptional
regulation
of
remodeling
in
response
to
stress.
How
mediated
by
the
CWI
effected
inputs
from
other
signaling
pathways
not
well
understood.
Here,
we
demonstrate
that
Mck1
kinase
cooperates
with
Slt2,
MAP
pathway,
promote
thickening
glucose-starved
cells.
Integrative
analyses
transcriptome,
proteome
and
metabolic
profiling
indicate
required
accumulation
UDP-glucose
(UDPG),
substrate
β-glucan
synthesis,
through
activation
two
regulons:
Msn2/4-dependent
stress
Cat8-/Adr1-mediated
reprogram
dependent
on
SNF1
complex.
Analysis
phosphoproteome
suggests
similar
mammalian
Gsk-3
kinases,
involved
cytoskeleton-dependent
cellular
processes,
metabolism,
transcription.
Specifically,
may
be
implicated
Snf1-dependent
PKA
inhibition
SAGA
(Spt-Ada-Gcn5
acetyltransferase)-mediated
transcription
activation,
a
hypothesis
further
underscored
significant
overlap
between
Mck1-
Gcn5-activated
transcriptomes.
Phenotypic
analysis
also
supports
roles
actin
cytoskeleton-mediated
exocytosis
ensure
plasma
membrane
homeostasis
wall-stressed
Together,
these
findings
only
reveal
novel
functions
reprogramming
polarized
growth
but
provide
valuable
omics
resources
future
studies
uncover
underlying
mechanisms
kinases
response.
Biotechnology and Bioengineering,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 11, 2025
Gradients
negatively
affect
performance
in
large-scale
bioreactors;
however,
they
are
difficult
to
predict
at
laboratory
scale.
Dynamic
microfluidics
single-cell
cultivation
(dMSCC)
has
emerged
as
an
important
tool
for
investigating
cell
behavior
rapidly
changing
environments.
In
the
present
study,
dMSCC,
biosensors
of
intracellular
parameters,
and
robustness
quantification
were
employed
investigate
physiological
response
three
Saccharomyces
cerevisiae
strains
substrate
pH
changes
every
0.75-48
min.
All
showed
higher
sensitivity
than
oscillations.
Strain-specific
responses
included
relative
glycolytic
flux
oxidative
stress
PE2
CEN.PK113-7D,
respectively.
Instead,
Ethanol
Red
strain
displayed
least
heterogeneous
populations
highest
multiple
functions
when
exposed
This
result
could
arise
from
a
positive
trade-off
between
ATP
levels
stability
over
time.
The
study
demonstrates
importance
coupling
dynamic
environments
with
simultaneous
characterization
strains,
conditions,
individual
regimes,
analysis.
these
tools
suitable
add-on
traditional
evaluation
screening
workflows
both
industrial
scale,
can
help
bridge
gap
two.
Journal of Agricultural and Food Chemistry,
Journal Year:
2019,
Volume and Issue:
67(34), P. 9551 - 9559
Published: Aug. 5, 2019
In
oleaginous
micro-organisms,
nitrogen
limitation
activates
adenosine
monophosphate
deaminase
(AMPD)
and
promotes
lipogenesis
via
the
inhibition
of
isocitrate
dehydrogenase.
We
found
that
overexpression
homologous
AMPD
in
Mortierella
alpina
favored
lipid
synthesis
over
cell
growth.
Total
fatty
acid
content
recombinant
strain
was
15.0–34.3%
higher
than
control,
even
though
their
biomass
similar.
During
early
fermentation
stage,
intracellular
AMP
level
reduced
by
40–60%,
together
with
a
1.9–2.7-fold
increase
citrate
compared
therefore
provided
more
precursors
for
synthesis.
Moreover,
decreased
resulted
metabolic
reprogramming,
reflected
blocked
TCA
cycle
reduction
amino
acids,
distributing
carbon
to
pathways.
By
coupling
energy
balance
lipogenesis,
this
study
provides
new
insights
into
metabolism
under
nitrogen-limited
conditions
targets
regulation
accumulation
micro-organisms.
FEMS Yeast Research,
Journal Year:
2019,
Volume and Issue:
20(1)
Published: Dec. 6, 2019
There
has
long
been
speculation
about
the
role
of
various
stresses
in
ageing.
Some
have
beneficial
effects
on
ageing-dependent
duration
and
severity
stress,
others
negative
question
arises
whether
these
are
causative
ageing
or
result
process.
Cellular
responses
to
many
highly
coordinated
a
concerted
way
hence
there
is
great
deal
cross-talk
between
different
stresses.
Here
relevant
aspects
coordination
stress
roles
yeast
cell
discussed,
together
with
functions
that
involved.
The
cellular
processes
involved
alleviating
considered,
possible
early
events
subsequent
cells.
Journal of Fungi,
Journal Year:
2023,
Volume and Issue:
9(8), P. 787 - 787
Published: July 26, 2023
Yeast
cells
are
equipped
with
different
nutrient
signaling
pathways
that
enable
them
to
sense
the
availability
of
various
nutrients
and
adjust
metabolism
growth
accordingly.
These
part
an
intricate
network
since
most
cross-regulated
subject
feedback
regulation
at
levels.
In
yeast,
a
central
role
is
played
by
Sch9,
protein
kinase
functions
as
proximal
effector
conserved
growth-regulatory
TORC1
complex
mediate
information
on
free
amino
acids.
However,
recent
studies
established
Sch9
more
than
TORC1-effector
its
activity
tuned
several
other
kinases.
This
allows
function
integrator
aligns
input
signals
achieve
accuracy
in
metabolic
responses
stress-related
molecular
adaptations.
this
review,
we
highlight
latest
findings
structure
well
nutrient-responsive
hub
impacts
longevity
yeast
cells.
Given
key
players
impinging
well-conserved,
also
discuss
how
can
be
instrumental
further
elucidate
mechanisms
underpinning
healthy
aging
mammalians.