Composition of Lignocellulose Hydrolysate in Different Biorefinery Strategies: Nutrients and Inhibitors

Molecules, Journal Year: 2024, Volume and Issue: 29(10), P. 2275 - 2275

Published: May 11, 2024

The hydrolysis and biotransformation of lignocellulose, i.e., biorefinery, can provide human beings with biofuels, bio-based chemicals, materials, is an important technology to solve the fossil energy crisis promote global sustainable development. Biorefinery involves steps such as pretreatment, saccharification, fermentation, researchers have developed a variety biorefinery strategies optimize process reduce costs in recent years. Lignocellulosic hydrolysates are platforms that connect saccharification downstream fermentation. hydrolysate composition closely related biomass raw pretreatment process, choice biorefining strategies, provides not only nutrients but also possible inhibitors for In this review, we summarized effects each stage lignocellulosic on inhibitors, analyzed huge differences nutrient retention inhibitor generation among various emphasized all lignocellulose need be considered comprehensively achieve maximum optimal control at low cost, reference development chemicals.

Language: Английский

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Mining novel gene targets for improving tolerance to furfural and acetic acid in Yarrowia lipolytica using whole-genome CRISPRi library

Bioresource Technology, Journal Year: 2024, Volume and Issue: 403, P. 130764 - 130764

Published: May 7, 2024

Language: Английский

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Sustainable lipid production by oleaginous yeasts: Current outlook and challenges

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132205 - 132205

Published: Feb. 1, 2025

Language: Английский

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Sustainable production of lipids from cocoa fatty acid distillate fermentation driven by adaptive evolution in Yarrowia lipolytica

Bioresource Technology, Journal Year: 2024, Volume and Issue: 394, P. 130302 - 130302

Published: Jan. 8, 2024

Language: Английский

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An Evolved Strain of the Oleaginous Yeast Rhodotorula toruloides, Multi-Tolerant to the Major Inhibitors Present in Lignocellulosic Hydrolysates, Exhibits an Altered Cell Envelope

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(11), P. 1073 - 1073

Published: Nov. 2, 2023

The presence of toxic compounds in lignocellulosic hydrolysates (LCH) is among the main barriers affecting efficiency lignocellulose-based fermentation processes, particular, to produce biofuels, hindering production intracellular lipids by oleaginous yeasts. These microbial oils are promising sustainable alternatives vegetable for biodiesel production. In this study, we explored adaptive laboratory evolution (ALE), under methanol- and high glycerol concentration-induced selective pressures, improve robustness a Rhodotorula toruloides strain, previously selected from sugar beet completely using major C (carbon) sources present. An evolved multi-tolerant not only methanol but four inhibitors present LCH (acetic acid, formic hydroxymethylfurfural, furfural) was isolated mechanisms underlying such multi-tolerance were examined, at cellular envelope level. Results indicate that strain has cell wall less susceptible zymolyase decreased permeability, based on propidium iodide fluorescent probe, absence or those inhibitors. improved performance lipid synthetic hydrolysate medium, supplemented with inhibitors, confirmed.

Language: Английский

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Optimal trade-off between boosted tolerance and growth fitness during adaptive evolution of yeast to ethanol shocks

Biotechnology for Biofuels and Bioproducts, Journal Year: 2024, Volume and Issue: 17(1)

Published: May 10, 2024

Abstract Background The selection of Saccharomyces cerevisiae strains with higher alcohol tolerance can potentially increase the industrial production ethanol fuel. However, design protocols to obtain bioethanol yeasts poses challenge improving that are already robust high levels. Furthermore, subjected mutagenesis and selection, or laboratory evolution, often present adaptation trade-offs wherein stress is attained at expense growth fermentation performance. Although these undesirable side effects associated acute regimes, utility using harsh treatments ethanologenic still has not been fully investigated. Results We conducted an adaptive evolution by challenging four populations (P1–P4) Brazilian yeast, PE-2_H4, through 68–82 cycles 2-h shocks (19–30% v/v) outgrowths. Colonies isolated from final evolved (P1c–P4c) were whole-genome sequencing, revealing mutations in genes enriched for cAMP/PKA trehalose degradation pathways. Fitness analyses clones P1c–P3c reverse-engineered demonstrated primarily selected cell viability under stress, cost decreased rates cultures without ethanol. Under this regime survival, population P4 a protective snowflake phenotype resulting BUD3 disruption. Despite marked trade-offs, combination cyr1 A1474T / usv1Δ conferred 5.46% fitness than parental PE-2_H4 propagation 8% (v/v) ethanol, only 1.07% culture medium alcohol. strain P1c displayed fermentations sugarcane molasses recycling sulfuric acid treatments, mimicking production. Conclusions Our study combined genomic, mutational, understand genetic underpinnings yeast shocks. revealed most impose propagation, key endowed presence Moreover, alleles survival comprising genotype optimal performance conditions simulating

Language: Английский

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Metabolic Engineering of Zymomonas mobilis for Xylonic Acid Production from Lignocellulosic Hydrolysate

Fermentation, Journal Year: 2025, Volume and Issue: 11(3), P. 141 - 141

Published: March 13, 2025

Bio-based xylonic acid produced from inexpensive lignocellulosic biomass has enormous market potential and enhances the overall economic benefits of biorefinery processes. In this study, introduction genes encoding xylose dehydrogenase driven by promoter Ppdc into Z. mobilis using a plasmid vector resulted in accumulation at titer 16.8 ± 1.6 g/L. To achieve stable production, gene cassette for production was integrated genome chromosomal locus ZMO0038 ZMO1650 endogenous type I-F CRISPR-Cas system. The resulting recombinant strain XA3 reduced to 12.2 0.56 g/L, which could be copy number difference between integration. Oxygen content then identified key factor production. further increase capability, strain, XA9, with five copies constructed integrating ZMO1094, ZMO1547, ZMO1577 on basis XA3. increased 51.9 0.1 g/L maximum yield 1.10 g/g, is close theoretical pure sugar medium. addition, XA9 genetically can produce 16.2 0.14 1.03 0.01 g/g hydrolysate. Our study thus hydrolysate, demonstrating capability as chassis biochemical

Language: Английский

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Evolutionary engineering and mutagenesis as alternatives to improve Spathaspora passalidarum for second-generation ethanol production

Biomass and Bioenergy, Journal Year: 2025, Volume and Issue: 198, P. 107885 - 107885

Published: April 14, 2025

Language: Английский

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Engineering Adaptive Alleles for Escherichia coli Growth on Sucrose Using the EasyGuide CRISPR System

Journal of Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Process strategies for enhanced sugar recovery from de-oiled rice bran for xylitol production using Pichia fermentans NCIM 3638

Biochemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 109633 - 109633

Published: Jan. 1, 2025

Language: Английский

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