The Serine Acetyltransferase (SAT) Gene Family in Tea Plant (Camellia sinensis): Identification, Classification and Expression Analysis under Salt Stress

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 9794 - 9794

Published: Sept. 10, 2024

Cysteine plays a pivotal role in the sulfur metabolism network of plants, intimately influencing conversion rate organic and plant’s capacity to withstand abiotic stresses. In tea serine acetyltransferase (SAT) genes emerge as crucial regulator cysteine metabolism, albeit with notable lack comprehensive research. Utilizing Hidden Markov Models, we identified seven CssSATs within plant genome. The results bioinformatics analysis indicate that these exhibit an average molecular weight 33.22 kD cluster into three distinct groups. Regarding gene structure, CssSAT1 stands out ten exons, significantly more than its family members. promoter regions, cis-acting elements associated environmental responsiveness hormone induction predominate, accounting for 34.4% 53.1%, respectively. Transcriptome data revealed intricate expression dynamics under various stress conditions (e.g., PEG, NaCl, Cold, MeJA) their tissue-specific patterns plants. Notably, qRT-PCR indicated salt stress, CssSAT3 levels markedly increased, whereas CssSAT2 displayed downregulatory trend. Furthermore, cloned CssSAT1-CssSAT3 constructed corresponding prokaryotic vectors. resultant recombinant proteins, upon induction, enhanced NaCl tolerance Escherichia coli BL21, suggesting potential application bolstering resistance. These findings have enriched our comprehension multifaceted roles played by mechanisms, laying theoretical groundwork future scientific endeavors research pursuits.

Language: Английский

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Genome-wide identification, molecular docking and expression analysis of enzymes involved in the primary and secondary metabolic branching points of the selenium metabolic pathway in Cardamine hupingshanensis

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 22, 2025

Cardamine hupingshanensis is a plant known for its unique selenium tolerance, making it key model metabolism research. Adenosine phosphosulfate kinase (APK) and adenosine reductase (APR) are widely distributed in plants play crucial role metabolism. While genome-wide analyses of the APK APR families have been conducted across various species, systematic identification analysis these gene still lacking. There 7 ChAPK 5 ChAPR genes identified from genome C. hupingshanensis, which can be classified into 4 subfamilies 3 ChAPR, respectively. All members share similar conserved motifs structures. Phylogenetic promoter suggest they involved environmental responses, phytohormone regulation, light signalling. Molecular docking indicated that enzymes higher affinity phosphoselenate (APSe) compared to ChAPR. In 3D interaction force analysis, residues such as His80, Asp134 Arg137 were found interact with substrate APSe ChAPK. For Gly226, Arg313 Leu150 primarily catalytic site. Leaf cells exhibited reduced state under 1 µg Se L- selenite concentration, but showed an oxidative at 100 1, by antioxidant enzyme activities metabolite assays. The expression level ChAPK2-1 ChAPR2 increased 4.2- 10.1-folds, respectively, cells, smaller increases state, only 1.5-fold 5.2-fold. strong regulated redox state. However, three (ChAPK1-1, ChAPK1-2, ChAPK4-2) located chloroplast. Furthermore, low concentrations nutrient solution promote activity leaves seedlings, whereas high exhibit opposite effect, confirmed results

Language: Английский

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Overlapping Transcriptome Alterations Reveal the Mechanism of Interaction between Selenium and Zinc and Their Common Effect on Essential Nutrient Metabolism in Mung Bean (Vigna radiata L.)

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(45), P. 25423 - 25435

Published: Nov. 4, 2024

Selenium (Se) and zinc (Zn) deficiencies have become serious global food security public health problems. Biofortification through foliar fertilizer is a nonspecific, low-tech, cost-effective strategy. Se Zn overlapping physiological roles interacting relationships in plants. Mung bean superior for enrichment an excellent carrier. However, the molecular mechanism underlying interaction between mung remains unclear. Herein, accumulation, antioxidant activities, determination, transcriptomic analysis were performed under both treatments. Common essential of reflected by comprehensively altered ten indexes Se2 (24 g·ha

Language: Английский

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Comprehensive Analysis of the OASTL Gene Family in Potato (Solanum tuberosum L.) and Its Expression Under Abiotic Stress

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 13170 - 13170

Published: Dec. 7, 2024

O-acetylserine (thiol) lyase is a pivotal enzyme in plant cysteine biosynthesis, which crucial for promoting growth, development, and resisting abiotic stress. However, the related studies on potato OASTL gene family (StOASTL) have not been reported. In present study, we identified 11 members of StOASTL family, conducting thorough analysis encompassing chromosome distribution, protein physicochemical properties, structure, protein-conserved motifs, replication events. Phylogenetic scrutiny delineated these StOASTLs into five distinct subfamilies. Using RNA-seq from Potato Genome Sequencing Consortium (PGSC), investigated expression profile

Language: Английский

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