Identification of Reference Gene for Quantitative Gene Expression in Early-Term and Late-Term Cultured Canine Fibroblasts Derived from Ear Skin DOI Creative Commons
Sang-Yun Lee, Yeonwoo Jeong, Yong‐Ho Choe

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(18), P. 2722 - 2722

Published: Sept. 20, 2024

Fibroblasts are cells that reside within the fibrous or loose connective tissues of most mammalian organs. For research purposes, fibroblasts often subjected to long-term culture under defined conditions, during which their properties can significantly change. It is essential understand and document these changes obtain reliable outcomes. quantification specific gene expressions, widely used technique quantitative real-time polymerase chain reaction (qRT-PCR). Here, we assessed impact a reference gene's stability on qRT-PCR analysis cultured canine skin fibroblasts. After successfully isolating from tissues, they were evaluated for proliferation β-galactosidase activity at different passage numbers. With extended culture, showed long doubling time elevated activity. Using three algorithms, geNorm, Normfinder, Bestkeeper, identified HPRT1, YWHAZ, GUSB as stable genes both early- late-passage Conventional such GAPDH found be less than those genes. The normalization Vimentin by statistical differences, whereas an unstable did not. Collectively, this study indicates using accurately reliably measuring expression in These findings provide valuable insights into internal controls studies expected utilized analyzing patterns molecular biology research.

Language: Английский

Circadian Rhythms in Cardiovascular Metabolism DOI Open Access
Hind Lal, Suresh K Verma, Yajing Wang

et al.

Circulation Research, Journal Year: 2024, Volume and Issue: 134(6), P. 635 - 658

Published: March 14, 2024

Energetic demand and nutrient supply fluctuate as a function of time-of-day, in alignment with sleep-wake fasting-feeding cycles. These daily rhythms are mirrored by 24-hour oscillations numerous cardiovascular functional parameters, including blood pressure, heart rate, myocardial contractility. It is, therefore, not surprising that metabolic processes also over the course day, to ensure temporal needs for ATP, building blocks, metabolism-based signaling molecules met. What has become increasingly clear is addition classic signal-response coupling (termed reactionary mechanisms), cardiovascular-relevant cells use autonomous circadian clocks temporally orchestrate pathways preparation predicted stimuli/stresses anticipatory mechanisms). Here, we review current knowledge regarding regulation metabolism, how synchronized function, whether misalignment/disruption contribute toward pathogenesis disease.

Language: Английский

Citations

12
Identification of Reference Gene for Quantitative Gene Expression in Early-Term and Late-Term Cultured Canine Fibroblasts Derived from Ear Skin DOI Creative Commons
Sang-Yun Lee, Yeonwoo Jeong, Yong‐Ho Choe

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(18), P. 2722 - 2722

Published: Sept. 20, 2024

Fibroblasts are cells that reside within the fibrous or loose connective tissues of most mammalian organs. For research purposes, fibroblasts often subjected to long-term culture under defined conditions, during which their properties can significantly change. It is essential understand and document these changes obtain reliable outcomes. quantification specific gene expressions, widely used technique quantitative real-time polymerase chain reaction (qRT-PCR). Here, we assessed impact a reference gene's stability on qRT-PCR analysis cultured canine skin fibroblasts. After successfully isolating from tissues, they were evaluated for proliferation β-galactosidase activity at different passage numbers. With extended culture, showed long doubling time elevated activity. Using three algorithms, geNorm, Normfinder, Bestkeeper, identified HPRT1, YWHAZ, GUSB as stable genes both early- late-passage Conventional such GAPDH found be less than those genes. The normalization Vimentin by statistical differences, whereas an unstable did not. Collectively, this study indicates using accurately reliably measuring expression in These findings provide valuable insights into internal controls studies expected utilized analyzing patterns molecular biology research.

Language: Английский

Citations

0