Differential effects of photophase on the reproductive behaviour of two species of medico‐legal relevance, Peckia (Peckia) chrysostoma (Wiedemann, 1830) and Peckia (Sarcodexia) lambens (Wiedemann, 1830) (Diptera: Sarcophagidae) DOI Open Access
Henrique Rafael Pontes Ferreira, Taciano Moura Barbosa, Simão Dias Vasconcelos

et al.

Medical and Veterinary Entomology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

The nocturnal behaviour and reproduction patterns of Sarcophagidae species during the scotophase are largely unexplored for in Neotropical region. aim this study was to assess light regimes under which females Peckia (Peckia) chrysostoma (Wiedemann, 1830) (Sarcodexia) lambens would larviposit understand how these variables influence intrauterine development flies. experiments were conducted an experimental room (lux = 100) two independent experiments: I. Larviposition test: Females mated with males over 8 days old. II. Intrauterine old individually isolated. Both performed photophases (L:D) 0:24, 6:18; 12:12; 18:6 24:0 a period 96 h. occurred total darkness P. (P.) (S.) all treatments, without significant differences among photophase treatments (p > 0.05). Photophase influenced timing larviposition, occurring as early 24 h 12:12 L:D conditions, while no larviposition observed first 0:24 treatment either species. duration significantly affected percentage gravid both < 0.05), higher percentages longer duration. at initial intermediate stages egg more strongly associated continuous (0:24) or short (6:18), whereas advanced stage prevalent treatment. implications findings forensic entomology profound, challenging conventional knowledge by revealing that necrophagous insects not limited diurnal activity patterns.

Language: Английский

Neuropeptidergic regulation of insect diapause by the circadian clock DOI Creative Commons
Charlotte Helfrich‐Förster

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 63, P. 101198 - 101198

Published: April 6, 2024

Diapause is an endocrine-mediated strategy used by insects to survive seasons of adverse environmental conditions. Insects living in temperate zones are regularly exposed such conditions the form winter. To winter, they must prepare for it long before arrives. A reliable indicator impending winter shortening day length. measure length, need their circadian clock as internal time reference. In this article, I provide overview current state knowledge on neuropeptides that link diapause inducing hormonal brain centers.

Language: Английский

Citations

8

A clock for all seasons DOI Creative Commons
Charlotte Helfrich‐Förster, Dirk Rieger

Journal of Comparative Physiology A, Journal Year: 2024, Volume and Issue: 210(4), P. 473 - 480

Published: June 19, 2024

Abstract Circadian clocks play an essential role in adapting locomotor activity as well physiological, and metabolic rhythms of organisms to the day-night cycles on Earth during four seasons. In addition, they can serve a time reference for measuring day length adapt advance annual changes environment, which be particularly pronounced at higher latitudes. The physiological responses are also known photoperiodism. This special issue Journal Comparative Physiology A aims account diurnal photoperiodic adaptations by presenting collection ten review articles, five original research three perspective pieces. contributions include historical accounts, circadian clock models, epigenetic, molecular, neuronal mechanisms seasonal adaptations, latitudinal differences studies wild that address challenges global change.

Language: Английский

Citations

4

Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae DOI Creative Commons

Yutaro Ohe,

Masaharu Hasebe, Yoshitaka Hamanaka

et al.

Journal of Comparative Physiology A, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Larvae of the flesh fly, Sarcophaga similis exhibit photoperiodic responses to control pupal diapause. Although external coincidence model is applicable S. photoperiodism, it remains unknown how circadian clock system integrates day-length information. To explore mechanisms, we examined neural circuitry involving lateral neurons (LNs) and prothoracicotropic hormone (PTTH) neurons. We also effects on LN-fiber patterns in third-instar larvae. Immunohistochemistry showed that protein PERIOD neuropeptide pigment-dispersing factor (PDF) were co-localized four cells per brain hemisphere, named these PDF-LNs similis. Single-cell polymerase chain reaction backfilled from ring gland two pairs pars lateralis with contralateral axons (PL-c neurons) expressed ptth. Double labeling immunohistochemistry backfills revealed PDF-immunoreactive varicose fibers projected close PL-c short f (snpf) receptor glutamate-gated chloride channel but not pdf sNPF L-glutamate PDF acutely inhibited spontaneous firing activity The number varicosities dorsal protocerebrum was significantly higher under short-day than long-day conditions a time-dependent manner. These results suggest and/or glutamate signaling PTTH form potential circuity for diapause photoperiod modifies connectivity strength between their post- or pre-neurons circuitry.

Language: Английский

Citations

0

A Head‐Specific Transcriptomic Study Reveals Key Regulatory Pathways for Winter Diapause in the Mosquito Culex pipiens DOI Open Access
Prabin Dhungana, Xueyan Wei, David Sung Yong Kang

et al.

Archives of Insect Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 118(2)

Published: Feb. 1, 2025

ABSTRACT The primary vector of the West Nile virus, Culex pipiens , undergoes reproductive dormancy during adverse winter season. While our current understanding has mainly focused on cellular signals and phenotypic shifts occurring at a global scale diapause, information tissue‐specific transcriptomic changes remains limited. This knowledge gap is major challenge in interpreting regulatory mechanisms tissue level. To address this, present work utilized RNA‐seq technology to investigate transcriptional head that house brain crucial endocrinal organs such as corpora allata. We obtained RNA samples from heads diapausing nondiapausing female mosquitoes two specific time intervals, ZT0 ZT16, then subjected them sequencing. Our results revealed differences differentially expressed genes between diapause non‐diapause highlighting diel variations gene expression. also selected twelve associated with phenotype examined transcript abundance six different points over 24 h. qRT‐PCR analysis showed similar up‐ downregulation transcripts nondiapause phenotypes thus validating RNA‐seq. In summary, study identified new differentiation their expression, potentially linking photoperiod seasonal insects. newly presented will significantly advance head‐specific for insect diapause.

Language: Английский

Citations

0

Erwin Bünning and Wolfgang Engelmann: establishing the involvement of the circadian clock in photoperiodism DOI Creative Commons
Charlotte Helfrich‐Förster

Journal of Comparative Physiology A, Journal Year: 2024, Volume and Issue: 210(4), P. 481 - 493

Published: May 28, 2024

In 1936, Erwin Bünning published his groundbreaking work that the endogenous clock is used to measure day length for initiating photoperiodic responses. His publication triggered years of controversial debate until it ultimately became basic axiom rhythm research and theoretical pillar chronobiology. Bünning's thesis frequently quoted in articles this special issue on subject "A all seasons". However, nowadays only few people know detail about experiments almost nobody knows contribution former doctoral student, Wolfgang Engelmann, theory because most topic German. The aim review give an overview important at time, including Engelmann's thesis, which he demonstrated importance circadian flower induction Flaming Katy, Kalanchoë blossfeldiana, but not Red Morning Glory, Ipomoea coccinea.

Language: Английский

Citations

3

One seasonal clock fits all? DOI Creative Commons
Stephan Michel, Laura Kervezee

Journal of Comparative Physiology A, Journal Year: 2023, Volume and Issue: 210(4), P. 641 - 647

Published: Nov. 10, 2023

Adaptation of physiology and behavior to seasonal changes in the environment are for many organisms essential survival. Most our knowledge about underlying mechanisms comes from research on photoperiodic regulation reproduction plants, insects mammals. However, even humans, who mostly live environments with minimal influences, show annual rhythms (e.g., immune activity, brain function), sleep-wake cycles) disease prevalence infectious diseases). As variations environmental conditions may be drastically altered due climate change, understanding adaptation becomes more relevant. While species have developed specific solutions dedicated tasks regulation, we find a number common principles when comparing insect mammalian systems: (1) circadian system contributes regulation; (2) similar signaling molecules (VIP PDF) used transferring information neuroendocrine controlling response; (3) hormone melatonin participates as well mammals; (4) photoperiod affect neurotransmitter function both animal groups. The few examples overlap elaborated this perspective article, discussion relevance should seen encouragement unravel machinery multitude organisms.

Language: Английский

Citations

5

Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae DOI Creative Commons

Yutaro Ohe,

Masaharu Hasebe, Yoshitaka Hamanaka

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 31, 2024

Abstract Larvae of the flesh fly, Sarcophaga similis exhibit photoperiodic responses to control pupal diapause. Although external coincidence model is applicable S. responses, it remains unknown how circadian clock system integrates day-length information. To explore mechanisms underlying diapause, we examined neural circuitry involving lateral neurons (LNs) and prothoracicotropic hormone (PTTH) neurons, latter possibly controlling ecdysteroid production. We also effects on LN-fiber patterns in third-instar larvae. Immunohistochemistry showed that PERIOD a neuropeptide pigment-dispersing factor (PDF) were co-localized four cells per hemisphere, named these PDF-LNs similis. Single-cell polymerase chain reaction backfilled from ring gland two pairs pars lateralis with contralateral axons (PL-c neurons) expressed ptth. Double labeling immunohistochemistry backfills revealed PDF-immunoreactive varicose fibers projected proximity PL-c neurons. However, pdf receptor was not but glutamate-gated chloride channel most L-glutamate PDF acutely inhibited spontaneous firing activity The number varicosities dorsal protocerebrum significantly higher under short-day than long-day conditions time-dependent manner. These results suggest PDF-LNs, PTTH glutamate signaling form potential circuity for diapause photoperiod modifies connectivity strength between their post- or pre-neurons circuitry.

Language: Английский

Citations

1

Clocks at a snail pace: biological rhythms in terrestrial gastropods DOI Creative Commons
Rodrigo B. Salvador, Barbara M. Tomotani

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e18318 - e18318

Published: Oct. 29, 2024

Biological rhythms are ubiquitous across the tree of life. Organisms must allocate their activities into moments day and season that will increase probability surviving reproducing, which is done in form daily annual rhythms. So far, vast majority studies on biological have focused classical laboratory model species. Still, use non-model species gaining traction, as part an effort to achieve a more holistic understanding clock/calendar mechanisms "real world" but this requires can be studied both lab nature. Terrestrial gastropods,

Language: Английский

Citations

1

Differential effects of photophase on the reproductive behaviour of two species of medico‐legal relevance, Peckia (Peckia) chrysostoma (Wiedemann, 1830) and Peckia (Sarcodexia) lambens (Wiedemann, 1830) (Diptera: Sarcophagidae) DOI Open Access
Henrique Rafael Pontes Ferreira, Taciano Moura Barbosa, Simão Dias Vasconcelos

et al.

Medical and Veterinary Entomology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

The nocturnal behaviour and reproduction patterns of Sarcophagidae species during the scotophase are largely unexplored for in Neotropical region. aim this study was to assess light regimes under which females Peckia (Peckia) chrysostoma (Wiedemann, 1830) (Sarcodexia) lambens would larviposit understand how these variables influence intrauterine development flies. experiments were conducted an experimental room (lux = 100) two independent experiments: I. Larviposition test: Females mated with males over 8 days old. II. Intrauterine old individually isolated. Both performed photophases (L:D) 0:24, 6:18; 12:12; 18:6 24:0 a period 96 h. occurred total darkness P. (P.) (S.) all treatments, without significant differences among photophase treatments (p > 0.05). Photophase influenced timing larviposition, occurring as early 24 h 12:12 L:D conditions, while no larviposition observed first 0:24 treatment either species. duration significantly affected percentage gravid both < 0.05), higher percentages longer duration. at initial intermediate stages egg more strongly associated continuous (0:24) or short (6:18), whereas advanced stage prevalent treatment. implications findings forensic entomology profound, challenging conventional knowledge by revealing that necrophagous insects not limited diurnal activity patterns.

Language: Английский

Citations

1