Galectin-3 depletion tames pro-tumoural microglia and restrains cancer cells growth DOI Creative Commons

Luis Cruz Hernández,

María Teresa Sánchez Montero,

Alberto Rivera-Ramos

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 15, 2023

ABSTRACT The glycoprotein Galectin-3 (Gal-3) is a multifunctional molecule that plays pivotal role in the initiation and progression of various central nervous system diseases, including cancer. Although involvement Gal-3 tumour progression, resistance to treatment immunosuppression has long been studied different cancer types, mainly outside system, its elevated expression myeloid glial cells underscores profound impact on brain’s immune response. In this context, microglia infiltrating macrophages, predominant non-cancerous within microenvironment, assume critical roles establishing an immunosuppressive milieu diverse brain tumours. Through utilisation primary cell cultures immortalised microglial lines, we have elucidated promoting migration, invasion, phenotypic activation. Furthermore, employing two distinct vivo models encompassing (glioblastoma) secondary tumours (breast metastasis), our histological transcriptomic analysis show depletion triggers robust pro-inflammatory response notably based interferon-related pathways. Interestingly, prominently observed tumour-associated macrophages (TAMs), resulting suppression growth.

Language: Английский

Genetically modified E. Coli secreting melanin (E.melanin) activates the astrocytic PSAP-GPR37L1 pathway and mitigates the pathogenesis of Parkinson’s disease DOI Creative Commons

Weixian Kong,

Yu Liu,

Pu Ai

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 10, 2024

The characteristic neuropathology of Parkinson's disease (PD) involves the abnormal accumulation phosphorylated α-synuclein (αSyn), as well a significant decrease in neuromelanin (NM) levels within dopamine neurons (DaNs). Unlike αSyn aggregates, relationship between NM and PD pathogenesis is not understood. In this study, we engineered an E. coli MG1655 strain to produce exosomes containing melanin (E.melanin), investigated its potential neuroprotective effects on DaNs context PD. By employing combination cell cultures, biochemical studies, single nuclear RNA sequencing (snRNA seq), various vivo validations, found that administration E.melanin effectively alleviated loss improved motor behavior impairments observed both pharmacological transgenic mouse models. Mechanistically, snRNA seq data suggested activated PSAP-GPR37L1 signaling pathway specifically astrocytes, leading reduction astrocytic engulfment synapses. Notably, activation GPR37L1 receptor using Tx14(A) peptide successfully rescued defects protected against degeneration mice with Overall, our findings provide novel insights into understanding molecular mechanisms underlying melanin's protective while offering strategies for manipulating treating pathophysiological progression.

Language: Английский

Citations

2
Dopaminergic neurons lacking Caspase-3 avoid apoptosis but undergo necrosis after MPTP treatment inducing a Galectin-3-dependent selective microglial phagocytic response DOI Creative Commons
Juan García‐Revilla, Rocío Ruiz, Ana M. Espinosa‐Oliva

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(8)

Published: Aug. 27, 2024

Language: Английский

Citations

1
Association Between Serum Galectin‐3 and Parkinson's Disease: A Two‐Sample Mendelian Randomization Study DOI Creative Commons
Rui Pan, Wei Li, Jinyuan Wang

et al.

Brain and Behavior, Journal Year: 2024, Volume and Issue: 14(10)

Published: Oct. 1, 2024

Parkinson's disease (PD) is a prevalent neurodegenerative disorder with poor prognosis. Observational studies have demonstrated significant correlation between serum galectin-3 and PD, suggesting potential role of as biomarker for PD. However, it still unclear whether contributes to the risk disease.

Language: Английский

Citations

1
Neuroprotective effect of rice bran enzymatic extract-supplemented diets in a murine model of Parkinson's disease DOI Creative Commons
Elena Gavilán,

Alicia Flores,

Angélica Castaño

et al.

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 116, P. 106220 - 106220

Published: May 1, 2024

Neurodegenerative disorders such as Parkinson's disease, present a global health concern with limited therapeutic options. In this context, dietary interventions have emerged potential strategy to counteract the oxidative stress and inflammation underlying these conditions. Rice bran enzymatic extract (RBEE), rich in bioactive compounds, has shown ability modulating neuroinflammatory responses improving mitochondrial function. This study investigates neuroprotective of RBEE murine model PD induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). The results reveal that supplementation effectively preserves dopaminergic population reduces MPTP-induced gliosis Substantia Nigra (SN). Moreover, enhances Complex I activity mouse brains. These findings underscore supplement mitigate neuronal loss, neuroinflammation, dysfunction associated PD. is promising novel candidate strategies, offering hope quest for effective preventive measures

Language: Английский

Citations

1
The Role of Galectin-3 in Retinal Degeneration and Other Ocular Diseases: A Potential Novel Biomarker and Therapeutic Target DOI Open Access
Ziyi Zhou,

Zhaochen Feng,

Xiaojia Sun

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(21), P. 15516 - 15516

Published: Oct. 24, 2023

Galectin-3 is the most studied member of Galectin family, with a large range mediation in biological activities such as cell growth, proliferation, apoptosis, differentiation, adhesion, and tissue repair, well pathological processes inflammation, fibrosis, angiogenesis. As known to all, aberrant neovascularization are main pathophysiological retinal degeneration many ocular diseases. Therefore, review aims conclude role Gal3 various diseases occurrence development discuss its molecular mechanisms according research systemic At same time, we summarized predictive biomarker clinical application inhibitors possibility novel target for treatment

Language: Английский

Citations

2
Higher Serum Galectin‑3 Levels Were Associated with More Severe Motor Performance in Parkinson’s Disease DOI Creative Commons
Jin Xu,

Hong-Fei Yu,

Qirong Wang

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 8207 - 8214

Published: Nov. 1, 2024

Background: Parkinson's disease (PD) is a movement disorder that lacks proven biomarkers. Case–control genome-wide association studies revealed the potential effect of galectin‑3 (GAL3) on motor progression in PD patients. Based this finding, our study aimed to explore correlation between serum GAL3 levels and performance Methods: Five hundred patients 200 healthy controls were recruited. The measured participants by enzyme linked immunosorbent assay (ELISA). baseline characteristics collected, associated scale scores obtained. Results: Compared with controls, greatly increased These could distinguish sensitivity 0.798 specificity 0.815 (AUC = 0.795, 95% CI 0.757– 0.834, P < 0.001). Patients age > 60 years tended have higher levels, duration, Hoehn-Yahr stage, MDS-UPDRS III total score, tremor subscores, rigid bradykinesia subscores than those ≤ years. When adjusting for confounders, level was significantly correlated score subscores. In men PD, score; but found women. Moreover, associations significant Conclusion: Higher related more severe years, it may be predictive biomarker Keywords: disease, Galectin‑3, Motor performance, Biomarker

Language: Английский

Citations

0
Obstructive sleep apnea, Galectin-3 and cognitive impairment DOI
Tomoyuki Kawada

Sleep Medicine, Journal Year: 2023, Volume and Issue: 111, P. 1 - 1

Published: Sept. 8, 2023

Language: Английский

Citations

0
Galectin-3 depletion tames pro-tumoural microglia and restrains cancer cells growth DOI Creative Commons

Luis Cruz Hernández,

María Teresa Sánchez Montero,

Alberto Rivera-Ramos

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 15, 2023

ABSTRACT The glycoprotein Galectin-3 (Gal-3) is a multifunctional molecule that plays pivotal role in the initiation and progression of various central nervous system diseases, including cancer. Although involvement Gal-3 tumour progression, resistance to treatment immunosuppression has long been studied different cancer types, mainly outside system, its elevated expression myeloid glial cells underscores profound impact on brain’s immune response. In this context, microglia infiltrating macrophages, predominant non-cancerous within microenvironment, assume critical roles establishing an immunosuppressive milieu diverse brain tumours. Through utilisation primary cell cultures immortalised microglial lines, we have elucidated promoting migration, invasion, phenotypic activation. Furthermore, employing two distinct vivo models encompassing (glioblastoma) secondary tumours (breast metastasis), our histological transcriptomic analysis show depletion triggers robust pro-inflammatory response notably based interferon-related pathways. Interestingly, prominently observed tumour-associated macrophages (TAMs), resulting suppression growth.

Language: Английский

Citations

0