Effects, methods and limits of the cryopreservation on mesenchymal stem cells

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 29, 2024

Language: Английский

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Exploring the therapeutic potential of different sources of mesenchymal stem cells: a novel approach to combat burn wound infections

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 29, 2024

The most prevalent and harmful injuries are burns, which still a major global health problem. Burn can cause issues because they boost the inflammatory metabolic response, organ malfunction systemic failure. On other hand, burn wound infection creates an environment that is conducive to growth of bacteria might put patient at risk for sepsis. In addition, scarring unavoidable, this results in patients having functional cosmetic issues. Wound healing amazing phenomenon with complex mechanism deals different types cells biomolecules. Cell therapy using stem one challenging treatment methods accelerates wounds. Since 2000, use mesenchymal (MSCs) regenerative medicine has increased. They be extracted from various tissues, such as bone marrow, fat, umbilical cord, amniotic membrane. According studies, cell wounds increases angiogenesis, anti-inflammatory properties, slows progression fibrosis, excellent ability differentiate regenerate damaged tissue. Figuring out main preclinical clinical problems stop people MSCs then suggesting right ways improve could help show benefits move cell-based forward. This review's objective was assess therapy's contribution promotion healing.

Language: Английский

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Mitigation of cyclophosphamide-induced premature ovarian insufficiency in rats through bone marrow-derived mesenchymal stem cells and placental extract therapy: a comprehensive evaluation

Beni-Suef University Journal of Basic and Applied Sciences, Journal Year: 2025, Volume and Issue: 14(1)

Published: Feb. 11, 2025

Abstract Objective Premature ovarian insufficiency (POI) is a significant reproductive health concern characterized by the depletion of primary follicles, often resulting from exposure to genotoxic agents. This study aimed explore therapeutic potential bone marrow-derived mesenchymal stem cells (BM-MSCs) and/or placental extracts (PE) in ameliorating POI induced cyclophosphamide, widely used chemotherapeutic agent. Materials and methods Thirty-five 8–10 weeks weight 200 ± 20 g female albino Sprague–Dawley (SD) rats were evenly distributed into five groups: negative control (Group 1), positive cyclophosphamide (CPA) 2), cell therapy (CPA+MSCs) 3), extract (CPA+PE) 4), combination (CPA+MSCs+PE) 5). Rats CPA group received intraperitoneal (IP) injection (50 mg/kg), followed daily (8 mg/kg) (14 days). Subsequently, 1 × 10 6 MSCs via intravenous administration 50 µL PE, sacrifice after 4 weeks. Flow cytometry was identify MSCs. Hormone levels (FSH, LH, E 2 , progesterone) assessed using ELISA, primordial follicles quantified evaluate follicle reserves. Ovarian structure histomorphologically evaluated, PCNA immunohistochemistry conducted. Morphometric measurements statistical analyses performed. Results Hormonal revealed decrease E2 progesterone levels, accompanied an increase FSH following treatment. However, BM-MSC therapy, hormonal nearly returned normal. In addition PE treatment also evaluated. resulted partial restoration balance, showing mild with slight reduction compared cyclophosphamide-treated group. Histological examination that caused loss stromal blood vessel damage, substantial fibrosis. Interestingly, showed some ameliorative effects on histology, reduced fibrosis preservation although these changes less pronounced than those observed alone. Conclusion more effective restoring folliculogenesis, whereas provided moderate protection against histological immunohistochemical alterations cyclophosphamide.

Language: Английский

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Bone Marrow Mesenchymal Stem Cells Promote Repairing the Bruised Tissue via Regulating mRNA Expression of Molecular Biomarkers and the Apoptotic Rate

Cellular Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 59(1), P. 1 - 20

Published: Jan. 10, 2025

Bruise is the extravasation of blood that may be mild or severe. Bone marrow mesenchymal stem cells (BM-MSCs) are one most promising used in regenerative medicine for treating many disorders. We aimed to evaluate efficiency BM-MSCs cutaneous bruises. 78 male albino rats were equally divided into 3 groups, control group (G1), bruise wound (G2) and Bruised animals treated with (G3). The sequences color changes recorded. Animals sacrificed skin samples collected histopathological examination analyzing mRNA expression rate transforming growth factor- β (TGF-β), interleukin-6 (IL-6), tumor necrotic factor-α (TNF-α), Heat shock protein-90 α (HSP-90α), Metalloprotiens-9 (MMP-9), microRNA-21 (miR-21), which incorporated healing process apoptotic rat. Subcutaneous injection reduced intensity bruised skin, statistically significant upregulation TGF-β, TNF-α, HSP-90α, down-regulation MMP-9 miR-21 rate, reduction inflammatory cells. have a improvement bruises by regulating TGF-β- IL-6- TNF-α- HSP-90α- MMP-9- reducing cell infiltration.

Language: Английский

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PLL-g-HPA Hydrogel Loaded Human Umbilical Cord Mesenchymal Stem Cells Promote Burn Wound Healing in Rat Model by Regulating Inflammation Response

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 779 - 789

Published: Jan. 1, 2025

Treatment of severe burn wound injury remains a significant clinical challenge as serious infections/complex repair process and irregulating inflammation response. Human umbilical cord mesenchymal stem cells (hUC-MSCs) have multidirectional differentiation potential could multiple injuries under appropriate conditions. Poly(L-lysine)-graft-4-hydroxyphenylacetic acid (PLL-g-HPA) hydrogel is an enzyme-promoted biodegradable in with good water absorption, biocompatibility anti-bacterial properties. Therefore, the aim this study was to evaluate therapeutic effect hUC-MSCs combined PLL-g-HPA on full thickness rat model. The developed characterized by Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), Hydrogen-1 nuclear magnetic resonance (H-NMR). cytotoxicity human foreskin fibroblasts (HFF) were assessed CCK-8 assay live/dead quantification antibacterial activity against Escherichia coli Staphylococcus aureus also detected colony forming unit. A full-thickness model 12 SD rats established, healing time/Histology/inflammation factor expression level. findings from SEM, FTIR, HFF analyses demonstrated successful synthesis hydrogels. These hydrogels exhibited low at minimal concentrations while maintaining excellent moisture retention Compared control group, treatment conjunction significantly enhanced healing, modulated inflammatory responses, promoted angiogenesis well re-epithelialization models. represents promising approach for management wounds.

Language: Английский

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Marine-derived secondary metabolites in oncology: A comprehensive review

Current Research in Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 100300 - 100300

Published: May 1, 2025

Language: Английский

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Advancements in Biomaterials for Regenerative Medicine Through Tissue Engineering in Quest of Sustainability

Regenerative Engineering and Translational Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: May 22, 2025

Language: Английский

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The Role and Mechanism of Dental Pulp Stem Cells in Highly Porous Microcarriers Forming New Micro-tissues to Accelerate Mandibular Bone Defect Repair

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 6, 2024

Abstract Objective Dental pulp stem cells (DPSCs) exhibit strong differentiation and proliferation capabilities but are often discarded. This experiment aimed to prepare PLGA (poly lactic-co-glycolic acid) highly open porous microspheres (HOPMS) form microscale cell carriers with DPSCs, exploring their efficacy mechanism in promoting alveolar bone defect repair. Methods Rat dental were cultured identified. HOPMS prepared porosity, pore size, degradation rate, PH change evaluated. Cytological experiments examined the effects of on proliferation, mineralized nodule formation, mineralization gene, inflammatory factor expression. Cells transduced lentivirus for fluorescence marking co-cultured carriers. Subcutaneous intraosseous implantations performed investigate biocompatibility, angiogenesis, response. Results The size diameter HOPM adjustable. promoted formation nodules, gene expression, exhibited no cytotoxicity. demonstrated that had excellent biocompatibility significant immune rejection. Intraosseous confirmed this material osteogenesis angiogenesis. Conclusion: loaded DPSCs effectively vascular repair, proving be an transplantation carrier.

Language: Английский

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Wound Healing, Metabolite Profiling, and In Silico Studies of Aspergillus terreus

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(10), P. 11681 - 11699

Published: Oct. 19, 2024

Burn injuries, which significantly affect global public health, require effective treatment strategies tailored to varying severity. Fungi are considered a sustainable, easily propagated source for lead therapeutic discovery. In this study, we explored the burn wound healing potential of

Language: Английский

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Exploration the Effects of Mesenchymal Stem Cells and Olive Leaf Extract on Physiological and Histopathological Changes on the Kidney of Diabetes Rats

Cellular Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 58(6), P. 807 - 822

Published: Dec. 28, 2024

Background/Aims: Gestational Diabetes Mellitus (GDM) is a common complication during pregnancy, defined as diabetes diagnosed in the second or third trimester, often asymptomatic. This study investigates therapeutic potential of olive leaf extracts and stem cells mitigating GDM-induced complications, particularly focusing on renal function, oxidative stress, pancreatic cell regeneration. Methods: Measurements were made gravid female rats with without intraperitoneal administration Streptozotocin (35 mg/kg body weight). Biochemical analyses conducted to evaluate function markers (urea, uric acid, creatinine) stress parameters (malondialdehyde, glutathione, superoxide dismutase levels). Histopathological immunohistopathological evaluations kidney tissues performed using hematoxylin eosin staining specific (p53, Insulin, PCNA) assess cellular changes. Results: The diabetic group exhibited significantly elevated levels urea, creatinine (p<0.01) compared control group. Treatment reduced these levels. Malondialdehyde but showed marked improvement treatment groups. Additionally, glutathione activities diminished (p<0.05) increased following treatment. revealed regeneration improved tissue morphology groups Conclusion: Stem exhibit significant ameliorating dysfunction, damage associated GDM, highlighting their role enhancing

Language: Английский

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