The Use of Wheat Starch as Gelling Agent for In Vitro Proliferation of Blackberry (Rubus fruticosus L.) Cultivars and the Evaluation of Genetic Fidelity after Repeated Subcultures DOI Creative Commons
Doina Clapa, Monica Hârța, Katalin Szabo

et al.

Horticulturae, Journal Year: 2023, Volume and Issue: 9(8), P. 902 - 902

Published: Aug. 8, 2023

Micropropagation has an important role in the large-scale production of blackberry plant material, given high proliferation rates this species. The aim present study was to evaluate proliferative capacity grown vitro on wheat starch-gelled culture medium compared classical agar-gelled and assess genetic fidelity between proliferated shoots their mother plants. Six varieties (‘Čačanska Bestrna’, ‘Chester Thornless’, ‘Driscoll’s Victoria’, ‘Loch Ness’, ‘Polar’, ‘Karaka Black’) were tested. For proliferation, Murashige Skoog (MS) supplemented with 0.5 mg dm−3 6-benzyladenine (BA) used. conventional gelled 0.5% agar, starch used as alternative gelling agent a concentration 5%. results showed that for all cultivars, highest number shoots/inoculum obtained medium, maximum value 54.42 ± 4.18 presented by Black’. Considering length shoots, tested cultivars outstanding 5% starch. values regarding observed Thornless’ followed ‘Čačanska Ness’ 5.55 0.04 cm, 5.46 0.06 5.37 0.09 respectively. uniformity micropropagated relation plants confirmed sequence-related amplified polymorphism (SRAP) start codon targeted (SCoT) molecular markers.

Language: Английский

Analysis of genetic diversity and population structure in some Egyptian Berseem (Trifolium alexandrinum) accessions based on ISSR, SCoT and SRAP markers DOI
Hagar Mostafa Mohamed,

Salah El-Din Sayed El-Assal,

A. Y. Gamal El-Din

et al.

Genetic Resources and Crop Evolution, Journal Year: 2024, Volume and Issue: unknown

Published: March 25, 2024

Language: Английский

Citations

3

Genetic diversity, chemical constituents and anatomical analysis of eight popular Olive (Olea europaea L.) cultivars in Al-Jouf region, Saudi Arabia DOI Creative Commons
Haifa A. S. Alhaithloul, Nabil S. Awad, Sameer H. Qari

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 26, 2024

Abstract In light of the multitude olive trees cultivated and lack genetic diversity available genotypes to select varieties lines that are characterized by high better performance under corresponding conditions, A comparison analysis genotyping morphological characteristics eight cultivars growing in Saudi Arabia’s Al-Jouf region was conducted analyzed. Morpho-anatomical chemical along with both inter-simple-sequence repeats (ISSRs) start-codon-targeted (SCoT) markers were used evaluate among Al-Jouf, Arabia. Analyses 27 morphological, chemical, anatomical concluded existence differences studied varieties. Moreover, six ISSR SCoT primer combinations produced a total 48 loci, which 18 (10 8 SCoT) polymorphic. The average polymorphism information content (PIC values 0.48 0.44, respectively) marker index (MI 0.79 0.48, detected for revealed prevalence Based on selected molecular markers, grouped into two distinct clusters. Clusters adjacent joint dendrogram using ISSR, combined data similar, all individuals groups. However, generated basis separated subgroups containing at least findings showed methods effective assessing diversity, can be as reliable informative method relationships serve complementary tool provide more complete understanding Olea europaea populations

Language: Английский

Citations

3

Juglans regia as Urban Trees: Genetic Diversity and Walnut Kernel Quality Assessment DOI Creative Commons

Alina-Maria Tenche-Constantinescu,

Dacian Lalescu, S. Popescu

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(10), P. 1027 - 1027

Published: Sept. 27, 2024

Juglans regia L. is an edible fruit tree cultivated worldwide for its fruits and wood as urban tree. Globally, there growing concern preserving the genetic diversity of trees with high economic ecological value. This study investigates J. in landscapes assesses quality walnut kernels a local food product. An inventory 150 from five populations public green spaces Lugoj, Caransebeș Jupa, well two semi-natural hilly ecosystems Banat Region, was conducted. Molecular analyses showed that Directed Amplification Minisatellite-region DNA (DAMD) markers were more effective than Start Codon Targeted (SCoT) markers, higher average polymorphism 56.26%, compared to 49.44%. DAMD07 achieved 100% DAMD05 strong balance between P.I.C. (0.35) (54.54%). Chemical analysis revealed following contents kernels: protein (12.81% 16.80%), lipids (60.39% 69.08%), total polyphenols (5484.66 10,788.4 mg GAE/kg), copper (3.655 8.532 mg/kg), manganese (14.408 28.618 zinc (19.813 46.583 lead (1.204 2.27 mg/kg) cadmium (0.03451 0.08065 mg/kg). These findings are critical conservation efforts, forestry management ensuring safety products derived regia.

Language: Английский

Citations

3

Implication of morphological and molecular studies on taxonomic treatment of Acer L. spp. and some selected taxa of Sapindaceae Juss. s.l. DOI
Mohamed A. Salim, Hussein Mm,

Nareman K. Hosney

et al.

Flora, Journal Year: 2025, Volume and Issue: unknown, P. 152675 - 152675

Published: Feb. 1, 2025

Language: Английский

Citations

0

The Use of Wheat Starch as Gelling Agent for In Vitro Proliferation of Blackberry (Rubus fruticosus L.) Cultivars and the Evaluation of Genetic Fidelity after Repeated Subcultures DOI Creative Commons
Doina Clapa, Monica Hârța, Katalin Szabo

et al.

Horticulturae, Journal Year: 2023, Volume and Issue: 9(8), P. 902 - 902

Published: Aug. 8, 2023

Micropropagation has an important role in the large-scale production of blackberry plant material, given high proliferation rates this species. The aim present study was to evaluate proliferative capacity grown vitro on wheat starch-gelled culture medium compared classical agar-gelled and assess genetic fidelity between proliferated shoots their mother plants. Six varieties (‘Čačanska Bestrna’, ‘Chester Thornless’, ‘Driscoll’s Victoria’, ‘Loch Ness’, ‘Polar’, ‘Karaka Black’) were tested. For proliferation, Murashige Skoog (MS) supplemented with 0.5 mg dm−3 6-benzyladenine (BA) used. conventional gelled 0.5% agar, starch used as alternative gelling agent a concentration 5%. results showed that for all cultivars, highest number shoots/inoculum obtained medium, maximum value 54.42 ± 4.18 presented by Black’. Considering length shoots, tested cultivars outstanding 5% starch. values regarding observed Thornless’ followed ‘Čačanska Ness’ 5.55 0.04 cm, 5.46 0.06 5.37 0.09 respectively. uniformity micropropagated relation plants confirmed sequence-related amplified polymorphism (SRAP) start codon targeted (SCoT) molecular markers.

Language: Английский

Citations

9