Primary omental smooth muscle tumor in an adult male: a diagnostic dilemma for leiomyoma: a case report DOI Creative Commons

Yukari Ono,

Yoichiro Okubo, Kota Washimi

et al.

Journal of Medical Case Reports, Journal Year: 2024, Volume and Issue: 18(1)

Published: May 5, 2024

Abstract Background The greater omentum comprises peritoneal, adipose, vascular, and lymphoid tissues. Most omental malignancies are metastatic tumors, the incidence of primary tumors is rare. We report on a prior smooth muscle tumor case in an adult male patient. Case presentation A 54-year-old Japanese patient with no relevant medical history was diagnosed abdominal mass during routine checkup. Subsequent contrast-enhanced computed tomography revealed approximately 3 cm size omentum, laparotomy performed. 27 × 25 20 mm raised lesion found omentum. Microscopically, spindle cells were observed arranged whorls fascicles. Individual had short spindle-shaped nuclei slightly increased chromatin characterized by eosinophilic, cytoplasm. mitotic count less than 1 per 50 high-power fields. showed positive immunoreactivity for α actin, HHF35, desmin immunohistochemical examination. Ki-67 labeling index using average method 1.76% (261/14806). No any other tested markers. considered leiomyoma owing to lack malignant findings. However, has rarely been reported, it can be difficult completely rule out potential soft Our decisively considering leiomyoma. Consequently, did not undergo additional adjuvant therapy followed up. satisfied treatment neither recurrence nor metastasis at 13-month postoperative follow-up. Discussion conclusion encountered histological findings suggestive malignancy extremely define as benign, requiring careful diagnosis. Further reports long-term follow-up series required determine whether true benign (leiomyoma) exists. In addition, proper interpretation should established. This study foundation future research.

Language: Английский

Effective Preparation of FFPE Tissue Samples for Preserving Appropriate Nucleic Acid Quality for Genomic Analysis in Thyroid Carcinoma DOI Creative Commons
Yoichiro Okubo,

Naoto Toyama,

Rika Kasajima

et al.

Endocrine Pathology, Journal Year: 2024, Volume and Issue: 35(4), P. 372 - 384

Published: Nov. 19, 2024

Abstract Formalin-fixed paraffin-embedded (FFPE) tissue samples are important for genomic analysis of thyroid carcinomas, particularly various molecularly targeted therapies. Therefore, this study developed and validated a technique preparing FFPE that preserves nucleic acid quality, which is fundamental precise analysis, more effectively than conventional methods. We analyzed surgically resected gland tumors, lymph node metastases, separately fixed tumor to optimize formalin fixation assess the influence specimen type preparation methods on quality. assessed several quality indicators, including DNA integrity number, cycle threshold ratio, RNA DV200. Separately consistently exhibited higher conventionally processed samples. Additionally, metastases often exhibit matching or exceeding highlighting their potential reliability analysis. These findings suggest utility types comprehensive genetic profiling carcinomas. In conclusion, demonstrated an effective method preserving analyses. Biopsy punches enable collection at facilities, those without ability handle frozen specimens. This contributes development obtaining high-quality pathological can be widely used in general medical practice. Moreover, equal superior as acceptable sources

Language: Английский

Citations

4
Identification of Oncogenic Alterations in 124 Cases of Pediatric Papillary Thyroid Carcinoma: BEND7::ALK, DLG5::RET, and CCDC30::ROS1 Fusions Induce MAPK Pathway Activation DOI
Yixuan Liu, Lei Bao, Guangqi Li

et al.

Endocrine Pathology, Journal Year: 2025, Volume and Issue: 36(1)

Published: Feb. 21, 2025

Language: Английский

Citations

0
Effect of storage temperature on nucleic acid quality in formalin-fixed paraffin-embedded tissue samples DOI Creative Commons
Yoichiro Okubo,

Naoto Toyama,

Rika Kasajima

et al.

Annals of Diagnostic Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 152496 - 152496

Published: May 1, 2025

Language: Английский

Citations

0
Primary omental smooth muscle tumor in an adult male: a diagnostic dilemma for leiomyoma: a case report DOI Creative Commons

Yukari Ono,

Yoichiro Okubo, Kota Washimi

et al.

Journal of Medical Case Reports, Journal Year: 2024, Volume and Issue: 18(1)

Published: May 5, 2024

Abstract Background The greater omentum comprises peritoneal, adipose, vascular, and lymphoid tissues. Most omental malignancies are metastatic tumors, the incidence of primary tumors is rare. We report on a prior smooth muscle tumor case in an adult male patient. Case presentation A 54-year-old Japanese patient with no relevant medical history was diagnosed abdominal mass during routine checkup. Subsequent contrast-enhanced computed tomography revealed approximately 3 cm size omentum, laparotomy performed. 27 × 25 20 mm raised lesion found omentum. Microscopically, spindle cells were observed arranged whorls fascicles. Individual had short spindle-shaped nuclei slightly increased chromatin characterized by eosinophilic, cytoplasm. mitotic count less than 1 per 50 high-power fields. showed positive immunoreactivity for α actin, HHF35, desmin immunohistochemical examination. Ki-67 labeling index using average method 1.76% (261/14806). No any other tested markers. considered leiomyoma owing to lack malignant findings. However, has rarely been reported, it can be difficult completely rule out potential soft Our decisively considering leiomyoma. Consequently, did not undergo additional adjuvant therapy followed up. satisfied treatment neither recurrence nor metastasis at 13-month postoperative follow-up. Discussion conclusion encountered histological findings suggestive malignancy extremely define as benign, requiring careful diagnosis. Further reports long-term follow-up series required determine whether true benign (leiomyoma) exists. In addition, proper interpretation should established. This study foundation future research.

Language: Английский

Citations

1