Hunger- and thirst-sensing neurons modulate a neuroendocrine network to coordinate sugar and water ingestion DOI Creative Commons

Amanda J González Segarra,

Gina Pontes, Nicholas Jourjine

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: June 15, 2023

Consumption of food and water is tightly regulated by the nervous system to maintain internal nutrient homeostasis. Although generally considered independently, interactions between hunger thirst drives are important coordinate competing needs. In Drosophila , four neurons called interoceptive subesophageal zone (ISNs) respond intrinsic signals oppositely regulate sucrose ingestion. Here, we investigate neural circuit downstream ISNs examine how ingestion based on Utilizing recently available fly brain connectome, find that synapse with a novel cell-type bilateral T-shaped neuron (BiT) projects neuroendocrine centers. vivo manipulations revealed BiT regulates sugar Neuroendocrine cells include several peptide-releasing peptide-sensing neurons, including insulin producing (IPCs), crustacean cardioactive peptide (CCAP) CCHamide-2 receptor isoform RA (CCHa2R-RA) neurons. These contribute differentially water, IPCs CCAP regulating ingestion, CCHa2R-RA modulating only Thus, decision consume or occurs via regulation broad peptidergic network integrates nutritional state generate nutrient-specific

Language: Английский

Insulin-Like Peptides and Cross-Talk With Other Factors in the Regulation of Insect Metabolism DOI Creative Commons
Szymon Chowański, Karolina Walkowiak‐Nowicka, Magdalena Joanna Winkiel

et al.

Frontiers in Physiology, Journal Year: 2021, Volume and Issue: 12

Published: June 29, 2021

The insulin-like peptide (ILP) and growth factor (IGF) signalling pathways play a crucial role in the regulation of metabolism, development, fecundity, stress resistance, lifespan. ILPs are encoded by multigene families that expressed nervous non-nervous organs, including midgut, salivary glands, fat body, tissue- stage-specific manner. Thus, more multidirectional complex control insect metabolism can occur. not only factors regulate metabolism. interact many cross-talk interactions different factors, for example, hormones (peptide nonpeptide), neurotransmitters factors. These observed at levels, three appear to be most prominent/significant: (1) coinfluence other on same target cells, (2) influence synthesis/secretion regulating (3) activity cells producing/secreting various For brain insulin-producing co-express sulfakinins (SKs), which cholecystokinin-like peptides, another key regulator express receptors tachykinin-related next involved It was also shown Drosophila melanogaster directly indirectly AKH. This review presents an overview regulatory peptides how these with players its regulation.

Language: Английский

Citations

67
Endocrine cybernetics: neuropeptides as molecular switches in behavioural decisions DOI Creative Commons
Dick R. Nässel, Meet Zandawala

Open Biology, Journal Year: 2022, Volume and Issue: 12(7)

Published: July 1, 2022

Plasticity in animal behaviour relies on the ability to integrate external and internal cues from changing environment hence modulate activity synaptic circuits of brain. This context-dependent neuromodulation is largely based non-synaptic signalling with neuropeptides. Here, we describe select peptidergic systems Drosophila brain that act at different levels a hierarchy associated physiology. These regions, such as central complex mushroom bodies, which supervise specific behaviours. At top level there are small numbers large neurons arborize widely multiple areas orchestrate or global state manner. bottom local provide executive sensory gain intrinsically restricted parts neuronal circuits. The orchestrating receive interoceptive signals mediate energy sleep homeostasis, metabolic circadian timing, well affect food search, aggression mating. Some these can be triggers conflicting behaviours mating versus aggression, feeding, participate circuits, enabling choices switches.

Language: Английский

Citations

55
Serotonergic neurons translate taste detection into internal nutrient regulation DOI Creative Commons
Zepeng Yao, Kristin Scott

Neuron, Journal Year: 2022, Volume and Issue: 110(6), P. 1036 - 1050.e7

Published: Jan. 19, 2022

The nervous and endocrine systems coordinately monitor regulate nutrient availability to maintain energy homeostasis. Sensory detection of food regulates internal in a manner that anticipates intake, but sensory pathways promote anticipatory physiological changes remain unclear. Here, we identify serotonergic (5-HT) neurons as critical mediators transform gustatory by into the activation insulin-producing cells enteric Drosophila. One class 5-HT responds sugars, excites cells, limits consumption, suggesting they anticipate increased levels prevent overconsumption. A second bitter compounds activates gastric motility, likely stimulate digestion increase circulating nutrients upon rejection. These studies demonstrate relay acute divergent for longer-term stabilization nutrients.

Language: Английский

Citations

42
Dietary cysteine drives body fat loss via FMRFamide signaling in Drosophila and mouse DOI Creative Commons
Tingting Song,

Wusa Qin,

Zeliang Lai

et al.

Cell Research, Journal Year: 2023, Volume and Issue: 33(6), P. 434 - 447

Published: April 13, 2023

Abstract Obesity imposes a global health threat and calls for safe effective therapeutic options. Here, we found that protein-rich diet significantly reduced body fat storage in fruit flies, which was largely attributed to dietary cysteine intake. Mechanistically, increased the production of neuropeptide FMRFamide (FMRFa). Enhanced FMRFa activity simultaneously promoted energy expenditure suppressed food intake through its cognate receptor (FMRFaR), both contributing loss effect. In body, signaling lipolysis by increasing PKA lipase activity. sweet-sensing gustatory neurons, appetitive perception hence We also demonstrated worked similar way mice via FF (NPFF) signaling, mammalian RFamide peptide. addition, or FMRFa/NPFF administration provided protective effect against metabolic stress flies without behavioral abnormalities. Therefore, our study reveals novel target development therapies obesity related diseases.

Language: Английский

Citations

28
The spatial and temporal structure of neural activity across the fly brain DOI Creative Commons
Evan Schaffer, Neeli Mishra, Matthew R Whiteway

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Sept. 11, 2023

What are the spatial and temporal scales of brainwide neuronal activity? We used swept, confocally-aligned planar excitation (SCAPE) microscopy to image all cells in a large volume brain adult Drosophila with high spatiotemporal resolution while flies engaged variety spontaneous behaviors. This revealed neural representations behavior on multiple scales. The activity most neurons correlated (or anticorrelated) running flailing over timescales that ranged from seconds minute. Grooming elicited weaker global response. Significant residual not directly was dimensional reflected small clusters spatially organized may correspond genetically defined cell types. These participate dynamics, indicating reflects combination local broadly distributed components. suggests microcircuits highly specified functions provided knowledge larger context which they operate.

Language: Английский

Citations

27
Leptin- and cytokine-like unpaired signaling in Drosophila DOI
Meet Zandawala, Jayati Gera

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 584, P. 112165 - 112165

Published: Jan. 23, 2024

Language: Английский

Citations

9
Synaptic connectome of the Drosophila circadian clock DOI Creative Commons
Nils Reinhard,

Ayumi Fukuda,

Giulia Manoli

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 5, 2024

Abstract The circadian clock and its output pathways play a pivotal role in optimizing daily processes. To obtain insights into how diverse rhythmic physiology behaviors are orchestrated, we have generated comprehensive connectivity map of an animal using the Drosophila FlyWire brain connectome. Intriguingly, identified additional dorsal neurons, thus showing that network contains ~240 instead 150 neurons. We revealed extensive contralateral synaptic within discovered novel indirect light input to also elucidated via which modulates descending neurons known regulate feeding reproductive behaviors. Interestingly, observed sparse monosynaptic between downstream higher-order centers neurosecretory cells behavior physiology. Therefore, integrated single-cell transcriptomics receptor mapping decipher putative paracrine peptidergic signaling by Our analyses neuropeptides expressed suggest significantly enriches interconnectivity network.

Language: Английский

Citations

9
Neuropeptidergic regulation of insect diapause by the circadian clock DOI Creative Commons
Charlotte Helfrich‐Förster

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 63, P. 101198 - 101198

Published: April 6, 2024

Diapause is an endocrine-mediated strategy used by insects to survive seasons of adverse environmental conditions. Insects living in temperate zones are regularly exposed such conditions the form winter. To winter, they must prepare for it long before arrives. A reliable indicator impending winter shortening day length. measure length, need their circadian clock as internal time reference. In this article, I provide overview current state knowledge on neuropeptides that link diapause inducing hormonal brain centers.

Language: Английский

Citations

8
Aminergic and peptidergic modulation of Insulin-Producing Cells in Drosophila DOI Open Access
Martina Held, Rituja S Bisen, Meet Zandawala

et al.

Published: Feb. 10, 2025

Insulin plays a critical role in maintaining metabolic homeostasis. Since demands are highly dynamic, insulin release needs to be constantly adjusted. These adjustments mediated by different pathways, most prominently the blood glucose level, but also feedforward signals from motor circuits and neuromodulatory systems. Here, we analyze how inputs control activity of main source Drosophila – population Insulin-Producing Cells (IPCs) located brain. IPCs functionally analogous mammalian pancreatic beta cells, their location makes them accessible for vivo recordings intact animals. We characterized functional using single-nucleus RNA sequencing analysis, anatomical receptor expression mapping, connectomics, an optogenetics-based ‘intrinsic pharmacology’ approach. Our results show that IPC expresses variety receptors neuromodulators classical neurotransmitters. Interestingly, exhibit heterogeneous profiles, suggesting can modulated differentially. This is supported electrophysiological IPCs, which performed while activating populations modulatory neurons. analysis revealed some have effects on activity, such they inhibit one subset exciting another. Monitoring calcium across uncovered these responses occur simultaneously. Certain shifted towards excited state, others it inhibition. Taken together, provide comprehensive, multi-level neuromodulation insulinergic system .

Language: Английский

Citations

1
Unveiling the sensory and interneuronal pathways of the neuroendocrine connectome in Drosophila DOI Creative Commons
Sebastian Hückesfeld, Philipp Schlegel, Anton Miroschnikow

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: June 2, 2021

Neuroendocrine systems in animals maintain organismal homeostasis and regulate stress response. Although a great deal of work has been done on the neuropeptides hormones that are released act target organs periphery, synaptic inputs onto these neuroendocrine outputs brain less well understood. Here, we use transmission electron microscopy reconstruction whole central nervous system Drosophila larva to elucidate sensory pathways interneurons provide input neurosecretory cells projecting endocrine organs. Predicted by network modeling, also identify new carbon dioxide-responsive acts specific set includes those expressing corazonin (Crz) diuretic hormone 44 (Dh44) neuropeptides. Our analysis reveals neuronal architecture for combinatorial action based interneuronal converge distinct combinations outputs.

Language: Английский

Citations

42