Research Square (Research Square),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Dec. 15, 2022
Abstract
Background
Numerous
distinct
secretagogues
such
as
(glyco)
proteins
including
the
GPI-anchored
are
added
to
sperm
surface
during
their
transit
through
male
reproductive
tract
(MRT).
This
remodelling
of
is
critical
for
maturation,
survival
and
function
in
female
(FRT).
study
aimed
characterize
present
on
buffalo
surface.
Results
A
surface-specific
proteomic
fingerprint
was
generated
using
shotgun
proteomics
(LC-MS/MS).
The
protein
informatics
platform,
Proteome
Discoverer
(v2.2)
identified
1342,
678,
982
isoforms
(P
<
0.05,
FDR
0.01)
salt-extracted,
PI-PLC
treated
capacitated
samples,
respectively.
Overall,
1695
unique
(minimum
2
peptides)
with
≥
1
high-quality
PSM/s
(proteoforms)
were
identified.
Descriptive
statistical
analysis
indicated
that
these
buffalo-specific
proteoforms
exhibit
remarkable
heterogeneity
molecular
weight,
pI,
distribution
expression
from
genome
functional
roles
MRT
FRT.
Subsequent
a
thorough
literature
search
revealed
fertility-related,
reproduction-specific
constituted
more
than
50%
(873)
sperm-surface
proteome
(1695).
Discussion
These
since
database,
NCBI
reference
(translated
latest
chromosome
level
assembly,
(UOA_WB_1)
used
space.
mapped
252
implicated
regulation
various
aspects
physiology
across
multiple
species.
Besides,
200
orphan,
(undefined
locus,
uncharacterized,
P
also
our
computational
strategy.
allowed
us
consider
novel
considered
mapping
ontology
terms.
led
elucidation
biological
functions
hitherto
unreported,
by
extrapolation
sequence
orthologs
several
ruminant
non-ruminant
(e.g.
Primates
Rodents)
mammalian.
uncharacterized
constitute
an
extensive,
yet
unexplored,
repertoire.
Conclusions
signature
driving
production,
discovered
this
unparalleled
vis-à-vis
depth
identification
fertility-related
cell-surface
proteins.
results
would
facilitate
advances
understanding
mammalian
function.
Frontiers in Endocrinology,
Journal Year:
2024,
Volume and Issue:
15
Published: Oct. 4, 2024
The
reproductive
efficiency
of
livestock
is
crucial
for
agricultural
productivity
and
economic
sustainability.
One
critical
factor
in
successful
fertilization
the
viability
offspring
quality
semen.
Poor
semen
quality,
especially
frozen-thawed
used
artificial
insemination
(AI)
have
been
shown
to
influence
conception
outcomes,
resulting
a
negative
impact
on
production.
Recent
advancements
genetic
research
identified
specific
markers
linked
traits
various
species,
such
as
cattle,
sheep,
goats,
pigs,
buffalo,
equines.
These
are
essential
screening
males
breeding
suitability,
which
turn
enhances
selective
programs.
Understanding
these
improving
performance
increasing
populations.
This
review
offers
comprehensive
overview
associated
with
key
livestock.
It
explores
underlying
mechanisms
their
practical
implications
animal
management.
underscores
importance
integrating
insights
into
strategies
optimize
ensure
sustainable
development
industries.
Frontiers in Cell and Developmental Biology,
Journal Year:
2023,
Volume and Issue:
11
Published: Feb. 20, 2023
The
water
buffalo
(Bubalus
bubalis)
is
an
indispensable
part
of
the
Indian
dairy
sector
and
in
several
instances,
farmers
incur
economic
losses
due
to
failed
pregnancy
after
artificial
insemination
(AI).
One
key
factors
for
failure
conception
use
semen
from
bulls
low
fertilizing
potential
hence,
it
becomes
important
predict
fertility
status
before
performing
AI.
In
this
study,
global
proteomic
profile
high
fertile
(HF)
(LF)
bull
spermatozoa
was
established
using
a
high-throughput
LC-MS/MS
technique.
A
total
1,385
proteins
(≥1
high-quality
PSM/s,
≥1
unique
peptides,
p
<
0.05,
FDR
0.01)
were
identified
out
which,
1,002
common
between
both
HF
LF
groups
while
288
95
respectively.
We
observed
211
342
significantly
(log
Fc
≥
2)
abundant
≤
0.5)
(p
0.05).
Gene
ontology
analysis
revealed
that
associated
involved
spermatogenesis,
sperm
motility,
acrosome
integrity,
zona
pellucida
binding
other
functions.
Besides
this,
glycolysis,
fatty
acid
degradation
inflammation.
Furthermore,
related
differentially
(DAPs)
on
viz.,
AKAP3,
Sp17,
DLD
validated
through
Western
blotting
immunocytochemistry
which
coherence
with
data.
DAPs
study
may
be
used
as
protein
candidates
predicting
buffaloes.
Our
findings
provide
opportunity
mitigating
male
infertility.
Frontiers in Cell and Developmental Biology,
Journal Year:
2021,
Volume and Issue:
9
Published: May 28, 2021
Metabolomic
approaches
allow
the
study
of
downstream
gene
expression
events
since
metabolites
are
considered
as
products
cell
signaling
pathways.
For
this
reason,
many
studies
in
humans
have
already
been
conducted
to
determine
influence
present
seminal
plasma
(SP)
on
sperm
physiology,
and
identify
putative
biomarkers.
However,
livestock
species,
these
relationships
yet
be
uncovered.
Thus,
aimed
explore:
(i)
if
concentrations
pig
SP
related
quality
functionality,
(ii)
they
could
predict
resilience
liquid
storage
at
17°C.
To
end,
28
ejaculates
were
individually
collected
split
into
three
aliquots:
one
was
used
for
analysis
through
nuclear
magnetic
resonance
(NMR)
spectroscopy;
another
served
evaluation
concentration
morphology;
last
utilized
functionality
parameters
using
computer-assisted
(CASA)
flow
cytometry
after
0
h
72
liquid-storage
NMR
allowed
identification
quantification
23
which,
except
fumarate,
not
observed
follow
a
breed-dependent
behavior.
Moreover,
specific
between
variables
identified:
glutamate,
methanol,
trimethylamine
N-oxide,
carnitine,
isoleucine
seen
some
evaluated
immediately
semen
collection;
leucine,
hypotaurine,
carnitine
found
associated
ability
withstand
storage;
(iii)
Bayesian
multiple
regression
models
metabolite
patterns
both
h.
The
opens
up
possibility
further
investigating
potential
functional
Frontiers in Genetics,
Journal Year:
2022,
Volume and Issue:
13
Published: Aug. 4, 2022
Trehalose
is
commonly
used
as
an
impermeable
cryoprotectant
for
cryopreservation
of
cells,
but
its
cryoprotective
mechanism
has
now
not
been
determined.
This
study
investigated
the
impact
trehalose
on
buck
semen
and
finished
metabolic
profiling
freeze-thawed
media
by
way
GC-MS-based
metabolomics
first
time.
Metabolic
pattern
recognition
metabolite
identification
means
principal
component
analysis
(PCA),
partial
least
squares
discriminant
(PLS-DA)
pathway
topology
revealed
results
sperm
metabolism
at
some
point
cryopreservation.
The
confirmed
that
drastically
progressed
motility
parameters
structural
integrity
after
thawing.
PCA
PLS-DA
discovered
patterns
freezing-thawing
cryopreserved
with
(T
group)
or
without
(G
group,
Control)
were
certainly
separated.
Using
screening
conditions
VIP
>1.5
p
vaule
<0.05,
a
total
48
differential
metabolites
have
recognized,
whithin
l-isoleucine,
L-leucine,
L-threonine,
dihydroxyacetone
notably
enriched
in
valine,
leucine
isoleucine
biosynthesis,
glycerolipid
metabolism,
aminoacyl-tRNA
biosynthesis
pathways.
In
brief,
can
efficiently
improve
membrane
motion
thawing,
it
exerts
aid
changing
amino
acid
synthesis
glycerol
pathway.
Experimental Eye Research,
Journal Year:
2023,
Volume and Issue:
239, P. 109724 - 109724
Published: Nov. 18, 2023
Diabetic
macular
edema
(DME)
is
the
most
common
cause
of
blindness
in
patients
with
diabetic
retinopathy.
To
investigate
proteomic
profiles
aqueous
humor
(AH)
individuals
(DME),
AH
samples
were
collected
from
non-diabetes
mellitus
(NDM),
DM,
nonproliferative
retinopathy
(NPDR),
and
DME.
We
performed
comparative
analyses
using
liquid
chromatography-tandem
mass
spectrometry
(LC-MS/MS)
bioinformatics
analyses.
identified
425
proteins
these
samples,
which
113
showed
changes
expression
DME
compared
NDM,
95
vs.
84
NPDR.
The
analysis
suggested
that
closely
associated
platelet
degranulation,
oxidative
stress-related
pathway,
vascular-related
pathways.
Upregulation
haptoglobin
(HP)
downregulation
fibrillin
1
(FBN1)
validated
by
ELISA.
Receiver
operating
characteristic
(ROC)
HP
FBN1
could
distinguish
NPDR
areas
under
curve
0.987
(p
=
0.00608)
0.791
0.00629),
respectively.
findings
provide
potential
clues
for
further
molecular
mechanisms
development
new
treatments
may
be
key
therapeutic
targets
human
proteomics
dataset
generated
has
been
deposited
to
ProteomeXchange/iProX
Consortium
Identifier:
PXD033404/IPX0004353001.
Archives animal breeding/Archiv für Tierzucht,
Journal Year:
2024,
Volume and Issue:
67(2), P. 153 - 161
Published: April 11, 2024
Abstract.
Litter
size
is
one
of
the
key
factors
affecting
efficiency
sheep
breeding,
and
previous
studies
found
that
HRG,
FETUB,
GUCY1A1
genes
were
closely
related
to
litter
in
sheep.
This
experiment
aims
explore
polymorphisms
g.405442728A>G
locus
HRG
gene,
g.421655951C>T
FETUB
g.414050897G>C
gene
their
association
with
size.
The
MassARRAY®
single-nucleotide
polymorphism
(SNP)
genotyping
technique
was
used
detect
these
loci
five
breeds,
i.e.,
Small-tailed
Han
sheep,
Hu
Cele
black
Sunite
Bamei
mutton
In
addition,
between
also
analyzed.
results
showed
moderately
polymorphic
(0.25
<
PIC
<0.5)
both
monotocous
polytocous
breeds;
lowly
(PIC
<0.25)
≤
low
four
other
breeds
<0.25).
chi-squared
test
Hardy–Weinberg
equilibrium
state
(P>0.05).
g.421655951
C>T
(P>0.05)
disequilibrium
(P<0.05).
analysis
had
a
significant
impact
on
(P<0.05),
while
no
summary,
can
be
as
potential
molecular
markers
for
selection
