Probiotics Mixture, Prohep: a Potential Adjuvant for Low-Dose Sorafenib in Metabolic Dysfunction–Associated Steatotic Liver Disease–Associated Hepatocellular Carcinoma Suppression Through Modulating Gut Microbiota DOI Creative Commons
Fangfei Zhang, Emily Kwun Kwan Lo, Congjia Chen

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: May 23, 2025

Abstract Targeting gut microbiota is an innovative approach to mitigate the development of metabolic dysfunction-associated steatotic liver disease-associated hepatocellular carcinoma (MASLD-HCC). This study aims investigate effects Prohep, a probiotic mixture, both as prophylactic measure and adjuvant therapy for low-dose sorafenib. A MASLD-HCC mice model was established by diethylnitrosamine (DEN) injection with feeding high-fat high-cholesterol (HFHC) diet. Gut microbiome profiles were later identified through shotgun sequencing. Our findings demonstrated that Prohep supplementation effectively suppressed in mice. protective effect attributed modulation increased production short-chain fatty acids (SCFAs), propionate, valerate. also activated AMPK, which decreased lipogenesis, reduced lipid uptake, enhanced antioxidant enzyme expressions. Additionally, cancer proliferation pathway PI3K/mTOR inhibited response treatment. As therapy, improved efficacy sorafenib, indicated tumor counts, alleviated inflammation, hepatic superoxide dismutase (SOD) expression. The combination led butyrate production, contributing overall therapeutic effects, thanks modulatory Prohep. These results underscore Prohep’s anti-tumorigenic properties its potential enhance outcomes sorafenib highlights importance developing effective neoadjuvant therapies long-term management strategies MASLD-HCC.

Language: Английский

The gut microbiome across the lifespan: how diet modulates our microbial ecosystem from infancy to the elderly DOI
Hazem Golshany, Shahinaz Helmy, Nashwa F. S. Morsy

et al.

International Journal of Food Sciences and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 27

Published: Dec. 19, 2024

This comprehensive review examines the impact of dietary patterns on gut microbiome composition and diversity from infancy to old age, linking these changes age-related health outcomes. It investigates how develops across life stages, focusing influence factors. The explores early-life feeding practices, including breastfeeding formula feeding, shape infant microbiota have lasting effects. In elderly individuals, alterations in are associated with increased susceptibility infections, chronic inflammation, metabolic disorders cognitive decline. critical role diet modulating throughout is emphasised, particularly potential benefits probiotics fortified foods promoting healthy ageing. By elucidating mechanisms connecting food systems health, this provides insights into interventions that could enhance resilience improve outcomes lifespan.

Language: Английский

Citations

7
Structural Insights into Amelioration Effects of Quercetin and Its Glycoside Derivatives on NAFLD in Mice by Modulating the Gut Microbiota and Host Metabolism DOI
Zunji Shi, Ce Zhang, Hehua Lei

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2022, Volume and Issue: 70(46), P. 14732 - 14743

Published: Nov. 9, 2022

The sugar moieties of natural flavonoids determine their absorption, bioavailability, and bioactivity in humans. To explore structure-dependent bioactivities quercetin, isoquercetin, rutin, which have the same basic skeleton linking different moieties, we systemically investigated ameliorative effects dietary these on high-fat diet (HFD)-induced nonalcoholic fatty liver disease (NAFLD) mice. Our results revealed that isoquercetin exhibits strongest capability improving NAFLD phenotypes mice, including body weight gain, glucose intolerance, systemic inflammation comparison with quercetin rutin. At molecular level, markedly ameliorated dysfunction host metabolic disorders mice NAFLD. microbial three compounds, especially can effectively regulate gut microbiota composition, such as genera Akkermansia, Bifidobacterium, Lactobacillus, were significantly disrupted These comparative findings offer new insights into activities for treatment.

Language: Английский

Citations

28
Common dietary emulsifiers promote metabolic disorders and intestinal microbiota dysbiosis in mice DOI Creative Commons
Suraphan Panyod, Wei‐Kai Wu, Chih-Ting Chang

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: June 20, 2024

Abstract Dietary emulsifiers are linked to various diseases. The recent discovery of the role gut microbiota–host interactions on health and disease warrants safety reassessment dietary through lens microbiota. Lecithin, sucrose fatty acid esters, carboxymethylcellulose (CMC), mono- diglycerides (MDG) common with high exposure levels in population. This study demonstrates that esters induce hyperglycemia hyperinsulinemia a mouse model. CMC disrupt glucose homeostasis vitro insulin-resistance MDG impairs circulating lipid metabolism. All change intestinal microbiota diversity dysbiosis. do not impact mucus–bacterial interactions, whereas tends cause bacterial encroachment into inner mucus layer enhance inflammation potential by raising lipopolysaccharide. Our findings demonstrate concerns associated using emulsifiers, suggesting they could lead metabolic syndromes.

Language: Английский

Citations

6
Effects of different energy levels in low-protein diet on liver lipid metabolism in the late-phase laying hens through the gut-liver axis DOI Creative Commons
Hong Hu, Ying Huang,

Anjian Li

et al.

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 11, 2024

Abstract Background The energy/protein imbalance in a low-protein diet induces lipid metabolism disorders late-phase laying hens. Reducing energy levels the to adjust energy-to-protein ratio may improve fat deposition, but this also decreases performance of This study investigated mechanism by which different influences liver hens through enterohepatic axis guide feed optimization and nutrition strategies. A total 288 were randomly allocated normal-energy normal-protein group (positive control: CK) or 1 3 groups: low-energy (LL), (NL), high-energy (HL) groups. ratios CK, LL, NL, HL diets 0.67, 0.74, 0.77, 0.80, respectively. Results Compared with CK group, egg quality deteriorated increasing intake fed diet. Hens had significantly higher triglyceride, cholesterol, acetyl-CoA carboxylase, fatty acid synthase levels, lower hepatic lipase compared group. Liver transcriptome sequencing revealed that genes involved beta-oxidation ( ACOX1 , HADHA EHHADH ACAA1 ) downregulated, whereas related synthesis SCD FASN ACACA upregulated LL Comparison cecal microbiome showed an diet, Lactobacillus Desulfovibrio enriched, riboflavin was suppressed. Cecal metabolites most affected included several vitamins, such as (vitamin B 2 ), pantethine 5 derivative), pyridoxine 6 4-pyridoxic acid. Conclusion disorder due deficiencies vitamin originating from be underlying reason for accumulation Based on present study, we propose targeting derivative) might effective strategy improving

Language: Английский

Citations

5
Structural elucidation of a Acanthopanax senticosus polysaccharide CQ-1 and its hepatoprotective activity via gut health regulation and antioxidative defense DOI

Shuxin Zhou,

Xin Wen,

Yang Zhao

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 281, P. 136343 - 136343

Published: Oct. 5, 2024

Language: Английский

Citations

4
Polymethoxyflavone–Enriched Fraction from Ougan (Citrus reticulata cv. Suavissima) Attenuated Diabetes and Modulated Gut Microbiota in Diabetic KK-Ay Mice DOI
Jiebiao Chen, Yang Liu, Hui‐Xin Wang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(18), P. 6944 - 6955

Published: April 26, 2023

Diabetes mellitus is a serious, chronic disease worldwide; yet it largely preventable through physical activity and healthy diets. Ougan (Citrus reticulata cv. Suavissima) characteristic citrus variety rich in polymethoxyflavones. In the present study, anti-diabetic effects of polymethoxyflavone-enriched fraction from (OG-PMFs) were investigated. Diabetic KK-Ay mice supplemented with different doses OG-PMFs for 5 weeks. Our results demonstrated that exhibited robust protective against diabetes symptoms mice. The potential mechanisms may partially be attributed to restoration hepatic GLUT2 catalase expression. Notably, OG-PMF administration significantly altered gut microbiota composition diabetic KK-Ay, indicated by suppression metabolic disease-associated genera Desulfovibrio, Lachnoclostridium, Enterorhabdus, Ralstonia, implying might another target show effects. Taken together, our provided supplementation metabolic-protective PMFs highlighted hold great developed as functional food ingredient.

Language: Английский

Citations

10
Lactobacillus plantarum Reduces Polystyrene Microplastic Induced Toxicity via Multiple Pathways: A Potentially Effective and Safe Dietary Strategy to Counteract Microplastic Harm DOI

Liuting Shi,

Changyin Wu, Yimin Wang

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 489, P. 137669 - 137669

Published: Feb. 18, 2025

Language: Английский

Citations

0
Fiber and starch of Colocasia esculenta var. Mentawai ameliorate adiposity, dyslipidemia and gut dysbiosis in mice fed high fat diet DOI Open Access
Putra Santoso, Resti Rahayu,

Wardatul Aini

et al.

Journal of Research in Pharmacy, Journal Year: 2025, Volume and Issue: 29(1), P. 310 - 321

Published: March 3, 2025

Mentawai taro (Colocasia esculenta var. Mentawai, Araceae) corm is a staple food for local people in islands, West Sumatra, Indonesia. This study aimed to determine whether the incorporation of fiber and starch extracted from could improve adiposity plasma lipid profiles intestinal microbiota composition mice fed fatty diet. Adult male (n = 24) were assigned four groups diet treatments: normal (ND), high-fat (HFD), HFD supplemented with 20% or corm, respectively. After 12-week treatment, body weight, adipose tissues, investigated. The results showed that was capable substantially preventing excessive weight increase against HFD. Moreover, significantly suppress white tissue mass adipocyte hypertrophy while reduction brown hypertrophy. also effectively reduce total cholesterol, low-density lipoprotein-cholesterol, triglyceride levels. result indicated modulate diversity by promoting health beneficial taxa suppressing pathogenic taxa. Overall, effectivity managing detrimental effects outperformed corm. Hence, it be recommended as potent supplement combat diet-induced metabolic problems, particularly obesity, dyslipidemia, gut dysbiosis.

Language: Английский

Citations

0
Microbiota-derived H2S induces c-kit+ cDC1 autophagic cell death and liver inflammation in metabolic dysfunction-associated steatohepatitis DOI Creative Commons

Yuxiang Song,

Na Li,

Shang Jiang

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 5, 2025

Immune dysregulation-induced inflammation serves as a driving force in the progression of metabolic dysfunction-associated steatohepatitis (MASH), while underlying cellular and molecular mechanisms remain largely uncharted. A Western diet (WD) is employed to construct mouse models dysfunction associated steatotic liver disease (MASLD) or MASH. Mass cytometry identifies c-kit+ cDC1 subset whose frequency reduced livers mice patients with MASH compared healthy controls. Adoptive cell transfer protects Moreover, analysis gut microbe sequence shows that WD-fed MASLD/MASH exhibit microbiota dysbiosis, an elevated abundance H2S-producing Desulfovibrio_sp. Transplanting MASH-derived fecal flora, Desulfovibrio_sp., injecting H2S intraperitoneally into MASLD decreases c-kit+cDC1 population exacerbates inflammation. Mechanistically, induces autophagic death c-kit-dependent manner cDC-specific c-kit-/- Atg5-/- mice. We thus uncover microbiota-derived triggers ignites inflammatory cascade

Language: Английский

Citations

0
Exercise-driven gut microbiota alterations enhance colonization resistance against methicillin-resistant Staphylococcus aureus DOI Creative Commons
Tingting Xu, Dan Fang, Tianqi Xu

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(3), P. 115424 - 115424

Published: March 1, 2025

Highlights•Moderate exercise enhances gut colonization resistance against MRSA in mice•D. newyorkensis (L8) is the key bacterium mediating increased resistance•Probiotic strain L8 thwarts by competing for carbon source fucoseSummaryGut microbiota plays a crucial role resisting invasion of pathogens, particularly multidrug-resistant (MDR) bacteria, which pose significant threat to public health. While offers numerous health benefits, its impact on host remains largely unclear. In this study, we demonstrate that moderate significantly reduces methicillin-resistant Staphylococcus aureus (MRSA), clinically important MDR pathogen. Moreover, identify an understudied intestinal probiotic Dubosiella as critical factor exercise-induced MRSA. Mechanistically, deprivation fucose, essential growth and pathogenicity. This process relies high binding affinity pyruvate ILE257 site lactate dehydrogenase L8. Overall, our work highlights importance maintaining demonstrates protecting colonization.Graphical abstract

Language: Английский

Citations

0