Exploring the transcriptomic profile of human monkeypox virus via CAGE and native RNA sequencing approaches DOI Creative Commons
Gergely Ármin Nagy, Dóra Tombácz, István Prazsák

et al.

mSphere, Journal Year: 2024, Volume and Issue: 9(9)

Published: Aug. 27, 2024

In this study, we employed short- and long-read sequencing technologies to delineate the transcriptional architecture of human monkeypox virus identify key regulatory elements that govern its gene expression. Specifically, conducted a transcriptomic analysis annotate transcription start sites (TSSs) end (TESs) by utilizing Cap Analysis expression on Illumina platform direct RNA Oxford Nanopore technology device. Our investigations uncovered significant complexity in use alternative TSSs TESs viral genes. research, also detected promoter poly(A) signals associated with Additionally, identified novel genes both left right variable regions genome.IMPORTANCEGenerally, gaining insight into how is regulated offers insights mechanisms control life cycle. The recent outbreak has underscored necessity understanding basic biology causative agent. results are pivotal for constructing comprehensive atlas virus, providing valuable resources future studies.

Language: Английский

Real-time genomic surveillance for enhanced control of infectious diseases and antimicrobial resistance DOI Creative Commons
Marc Struelens, Catherine Ludden, Guido Werner

et al.

Frontiers in Science, Journal Year: 2024, Volume and Issue: 2

Published: April 25, 2024

This article advocates for mobilizing pathogen genomic surveillance to contain and mitigate health threats from infectious diseases antimicrobial resistance (AMR), building upon successes achieved by large-scale genome sequencing analysis of SARS-CoV-2 variants in guiding COVID-19 monitoring public responses adopting a One Health approach. Capabilities laboratory-based epidemic alert systems should be enhanced fostering (i) universal access real-time whole sequence (WGS) data pathogens inform clinical practice, infection control, policies, vaccine drug research development; (ii) integration diagnostic microbiology data, testing asymptomatic individuals, epidemiological into programs; (iii) stronger cross-sectorial collaborations between healthcare, health, animal environmental using approaches, toward understanding the ecology transmission pathways AMR across ecosystems; (iv) international collaboration interconnection networks, harmonization laboratory methods, standardization methods global reporting, including on variant or strain nomenclature; (v) responsible sharing databases, platforms according FAIR (findability, accessibility, interoperability, reusability) principles; (vi) system implementation its cost-effectiveness different settings. Regional policies governance initiatives foster concerted development efficient utilization protect humans, animals, environment.

Language: Английский

Citations

24
A Review of the Molecular Understanding of the Mpox Virus (MPXV): Genomics, Immune Evasion, and Therapeutic Targets DOI Creative Commons
Edgar Cambaza

Zoonotic Diseases, Journal Year: 2025, Volume and Issue: 5(1), P. 3 - 3

Published: Jan. 16, 2025

The Mpox virus (MPXV), a zoonotic pathogen from the Orthopoxvirus genus, has emerged as significant global public health concern, especially after unprecedented outbreak in 2022. This review synthesizes MPXV’s molecular features, focusing on its genomic structure, replication mechanisms, immune evasion strategies, and implications for diagnostics therapeutics. study examines virus’s organization utilizing recent peer-reviewed literature, highlighting essential genes like OPG027 D1L, which contribute to host adaptation, increased transmissibility, evasion. Advances diagnostics, including real-time PCR genome sequencing, are reviewed, emphasizing their critical role monitoring control. However, challenges persist, such diagnostic limitations resource-constrained settings lack of targeted vaccines antivirals. discusses new antiviral candidates, confirmed through computational vitro techniques, identifying thymidine kinase VP39 key therapeutic targets. Emphasizing need surveillance track adaptive evolution, results show that particular mutations, D1L genes, increase transmissibility MPXV. These revelations highlight urgent necessity better catered towards addressing present constraints developing focused treatments reduce effect virus. emphasizes how these underscore combined plans handle changing MPXV epidemiology properly.

Language: Английский

Citations

1
Mpox: A case study for a one health approach to infectious disease prevention DOI Creative Commons

David T. S. Hayman,

Marion Koopmans, Andrew A. Cunningham

et al.

One Health, Journal Year: 2025, Volume and Issue: unknown, P. 101059 - 101059

Published: May 1, 2025

Language: Английский

Citations

0
An overview of the progress made in research into the Mpox virus DOI
Yansheng Li, Lianrong Wang,

Shi Chen

et al.

Medicinal Research Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

Abstract Mpox is a zoonotic illness caused by the virus (MPXV), member of Orthopoxvirus family. Although few cases have been reported outside Africa, it was originally regarded as an endemic disease limited to African countries. However, outbreak 2022 remarkable in that infection spread more than 123 countries worldwide, causing thousands infections and deaths. The ongoing has declared public health emergency international concern World Health Organization. For better management control epidemic, this review summarizes research advances important scientific findings on MPXV reviewing current literature epidemiology, clinical characteristics, diagnostic methods, prevention treatment measures, animal models MPXV. This provides useful information raise awareness about transmission, symptoms, protective measures MPXV, serving theoretical guide for relevant institutions

Language: Английский

Citations

1
Exploring the Transcriptomic Profile of Human Monkeypox Virus via CAGE and Native RNA Sequencing Approaches DOI Creative Commons
Gergely Ármin Nagy, Dóra Tombácz, István Prazsák

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 1, 2024

Abstract In this study, we employed short- and long-read sequencing technologies to delineate the transcriptional architecture of human monkeypox virus identify key regulatory elements that govern its gene expression. Specifically, conducted a transcriptomic analysis annotate transcription start sites (TSSs) end (TESs) by utilizing cap expression on Illumina platform direct RNA Oxford Nanopore technology device. Our investigations uncovered significant complexity in use alternative TSSs TESs viral genes. research, also detected promoter poly(A) signals associated with Additionally, identified novel genes both left right variable regions genome. Importance Generally, gaining insight into how is regulated offers insights mechanisms control life cycle. The recent outbreak has underscored necessity understanding basic biology causative agent. results are pivotal for constructing comprehensive atlas virus, providing valuable resources future studies.

Language: Английский

Citations

0
Exploring the transcriptomic profile of human monkeypox virus via CAGE and native RNA sequencing approaches DOI Creative Commons
Gergely Ármin Nagy, Dóra Tombácz, István Prazsák

et al.

mSphere, Journal Year: 2024, Volume and Issue: 9(9)

Published: Aug. 27, 2024

In this study, we employed short- and long-read sequencing technologies to delineate the transcriptional architecture of human monkeypox virus identify key regulatory elements that govern its gene expression. Specifically, conducted a transcriptomic analysis annotate transcription start sites (TSSs) end (TESs) by utilizing Cap Analysis expression on Illumina platform direct RNA Oxford Nanopore technology device. Our investigations uncovered significant complexity in use alternative TSSs TESs viral genes. research, also detected promoter poly(A) signals associated with Additionally, identified novel genes both left right variable regions genome.IMPORTANCEGenerally, gaining insight into how is regulated offers insights mechanisms control life cycle. The recent outbreak has underscored necessity understanding basic biology causative agent. results are pivotal for constructing comprehensive atlas virus, providing valuable resources future studies.

Language: Английский

Citations

0