Acta Biomedica Scientifica (East Siberian Biomedical Journal),
Journal Year:
2024,
Volume and Issue:
9(6), P. 12 - 21
Published: Dec. 28, 2024
Background
.
Copper
ions
are
necessary
for
maintaining
basic
physiological
processes
in
the
mammalian
organism.
However,
their
excessive
absorption
or
accumulation
cells
can
lead
to
development
exacerbation
of
various
pathological
processes.
The
cytotoxic
and
genotoxic
effects
high
concentrations
copper
compounds
currently
well
studied
cell
cultures,
whereas
effect
non-toxic
amounts
on
cells,
including
during
cultivation,
has
been
extremely
poorly
studied.
aim
study
To
investigate
changes
intracellular
amount
mitochondrial
cytochrome
C
oxidase
glutathione
synthetase.
Materials
methods
A
primary
culture
hepatocytes
was
obtained,
which
exposed
acetate
at
a
concentration
200
µg/ml
terms
content
24
hours.
After
fixation,
samples
were
stained
immunocytochemically
using
antibodies
(CcO)
subunit
I
synthetase
(GS).
Results
In
hepatocyte
culture,
significant
increase
intensity
fluorescent
staining
two
analyzed
enzymes
demonstrated
both
after
6
hours
exposure
ions,
indicates
change
number
cells.
At
same
time,
CcO
more
intense
first
incubation
with
microelement,
next
18
hours,
less
pronounced.
GS
stain
active
observed
throughout
entire
cultivation
period.
Conclusion
From
results
it
be
concluded
that
able
influence
key
indicators
viability
by
changing
one
main
energy
metabolism
enzyme
provides
synthesis
most
important
low-molecular
antioxidant
glutathione.
Frontiers in Veterinary Science,
Journal Year:
2024,
Volume and Issue:
11
Published: Aug. 7, 2024
Oxidative
damage
resulting
from
weaning
stress
significantly
impacts
the
growth
performance
and
health
status
of
piglets.
Taurine,
a
dietary
antioxidant
with
diverse
functions,
was
investigated
in
this
study
for
its
protective
role
against
stress-induced
oxidative
underlying
mechanism.
Forty
28-day-old
male
castrated
weaned
piglets
were
randomly
assigned
to
four
groups.
The
control
group
received
basal
diet,
while
experimental
groups
fed
diet
supplemented
0.1,
0.2%,
or
0.3%
taurine
over
28-day
period.
Preventive Nutrition and Food Science,
Journal Year:
2025,
Volume and Issue:
30(1), P. 21 - 27
Published: Feb. 28, 2025
Oxidative
stress
is
an
important
contributor
to
degenerative
diseases
in
the
elderly.
Pumpkin
known
for
its
potent
antioxidant
properties.
The
present
study
evaluated
effects
of
two
types
instant
pumpkin
soups
on
capacity
and
oxidative
This
used
a
quasi-experimental
design
compare
soups:
soup
supplemented
with
chicken
breast
(IPB)
prepared
original
recipe
(IPO).
Plasma
antioxidants
[superoxide
dismutase
(SOD),
glutathione
peroxidase,
catalase]
markers
[malondialdehyde
(MDA),
oxidized
low-density
lipoprotein
(ox-LDL),
8-isoprostane,
tumor
necrosis
factor-α
(TNF-α),
8-hydroxy-2'-deoxyguanosine
(8-OHdG)]
were
analyzed
using
enzyme-linked
immunosorbent
assay.
results
showed
that
IPB
had
higher
protein
(13.40%
vs.
2.20%),
fiber
(13.9%
9.21%),
β-carotene
(6.28
mg/100
g
3.38
g),
vitamin
A
(96.63
µg/100
81.82
ash
contents
(5.68%
3.00%)
than
IPO.
Both
demonstrated
activity,
showing
greater
significantly
increased
plasma
SOD
(432.63±396.03
pg/mL;
P=0.011),
but
not
IPO
(127.30±364.69
P=0.326).
reduced
ox-LDL,
TNF-α,
8-OHdG
levels.
Compared
IPO,
decreased
MDA
levels
(P<0.05).
Reductions
ox-LDL
(-555.03±155.86
-83.09±66.45
P<0.001)
TNF-α
(-58.24±22.85
-30.59±18.08
ng/mL;
P=0.012)
group
group.
These
findings
suggest
may
serve
as
functional
food
reducing
damage
Current Issues in Molecular Biology,
Journal Year:
2025,
Volume and Issue:
47(4), P. 223 - 223
Published: March 26, 2025
Citrus
is
a
globally
consumed
fruit
with
great
popularity,
yet
systematic
analyses
of
primary
metabolites
across
major
varieties
remain
scarce,
phenolic
acids
as
an
auxiliary
focus
due
to
their
flavor
relevance.
In
this
study,
the
and
five
citrus
were
analyzed
via
liquid
chromatography‒tandem
mass
spectrometry
(LC–MS/MS).
The
analysis
revealed
that
different
contained
342
77
acids.
PCA
clearly
distinguished
various
varieties.
Compared
pomelo
group,
lemon
group
presented
most
differentially
abundant
metabolites,
whereas
kumquat
navel
orange
groups
fewest.
An
examination
metabolic
pathways
notable
disparities
phenylpropanoid
biosynthesis
amino
significantly
varied
between
This
study
elucidates
networks
underlying
diversification,
providing
secondary
modulation
insights.
Moreover,
provides
theoretical
foundation
for
enhancing
fruits.
Foods,
Journal Year:
2025,
Volume and Issue:
14(7), P. 1166 - 1166
Published: March 27, 2025
The
aim
of
this
study
is
to
establish
a
rapid
and
convenient
microwave-assisted
digestion
method
for
sample
pretreatment
evaluate
amino
acids
in
Chebulae
fructus
(CF).
microwave
was
optimized
extract
from
CF,
the
differences
CF
different
origins
processing
states
were
analyzed
evaluated.
influences
temperature,
time,
liquid-material
ratio
on
extraction
effect
investigated
by
sing
factor
test
response
surface
(RSM),
conditions
optimized.
contents
17
determined
an
automatic
acid
analyzer.
optimal
temperature
150
°C,
time
18
min,
65:1
(mL:g).
Under
these
conditions,
total
content
could
reach
19.72
mg/g.
Lincang
city
Yunnan
province
unprocessed
considered
have
higher
nutritional
value.
results
chemometric
analysis
showed
that
there
significant
between
Guangxi
province,
Dehong
prefecture
six
differential
three
screened
out.
This
can
provide
references
quality
evaluation
producing
area,
extraction,
research
CF.
Neuroreport,
Journal Year:
2025,
Volume and Issue:
36(6), P. 327 - 335
Published: March 26, 2025
Acute
cerebral
infarction
(CI)
is
characterized
by
acute
onset,
high
disability
rate,
and
morbidity
which
seriously
threatens
the
health
safety
of
people
places
a
heavy
burden
on
individuals
country.
This
study
aimed
to
explore
effects
butylphthalide
nerve
cell
ferroptosis
in
CI
rats
its
underlying
mechanisms.
Middle
artery
occlusion
(MCAO)
rat
model
was
used
effect
vivo
oxygen
glucose
deprivation
(OGD)
vitro
.
Our
findings
demonstrated
that
markedly
reduced
oxidative
damage
brains
MCAO
rats.
Furthermore,
we
found
upregulated
netrin-1/deleted
colorectal
cancer
(DCC)/vascular
endothelial
growth
factor
(VEGF)
signaling
axis,
regulates
NF-E2-related
factor-2
(NRF2)
expression
contributes
OGD-treated
HT22
cells.
Collectively,
our
indicate
inhibits
stress-mediated
cells
modulating
netrin-1/DCC/VEGF/NRF2
axis.
In
conclusion,
results
reveal
novel
mechanism
for
protection
CIs
butylphthalide.
