Phage Endolysins as an Alternative Biocontrol Strategy for Pathogenic and Spoilage Microorganisms in the Food Industry
Viruses,
Journal Year:
2025,
Volume and Issue:
17(4), P. 564 - 564
Published: April 14, 2025
Food
contamination
by
pathogenic
and
spoilage
bacteria
causes
approximately
47
million
cases
of
foodborne
diseases
in
the
United
States
leads
to
tons
food
spoilage,
worsening
loss
situation
worldwide.
In
addition,
conventional
preservation
treatments
implemented
industry
decrease
food’s
nutritional
organoleptic
quality.
Therefore,
there
is
a
need
for
new
alternatives
counteract
without
altering
its
characteristics.
Endolysins
are
promising
strategy
due
their
unique
properties,
such
as
host
specificity,
synergism
with
other
antibacterial
agents,
mode
action,
low
probability
resistance
development.
These
characteristics
differentiate
them
from
agents
used
industry.
enzymes
produced
bacteriophages
during
process
bacterial
infection
lysis.
This
review
describes
advances
related
endolysin
application
systems
food,
considering
potential
safety
an
overview
conditions
according
type
be
controlled.
We
also
highlight
studies
on
encapsulation
prolongation
action
time
outbreaks
that
allow
obtaining
key
information
improve
endolysins
different
matrices
processing
storage
Language: Английский
Effects of Fucoidan on the Inhibition of Biofilm Formation of Salmonella enterica Subsp. enterica Serovar Typhimurium on Seafoods and Its Molecular Antibiofilm Mechanisms
Microorganisms,
Journal Year:
2025,
Volume and Issue:
13(4), P. 914 - 914
Published: April 16, 2025
Foodborne
illnesses,
particularly
those
caused
by
Salmonella
enterica
subsp.
Serovar
Typhimurium,
present
a
significant
challenge
to
public
health,
especially
within
the
seafood
industry
due
biofilm
formation
on
foods.
This
study
investigated
antibiofilm
potential
of
fucoidan,
sulfated
polysaccharide,
against
Typhimurium
crab
and
shrimp
surfaces.
Fucoidan’s
minimum
inhibitory
concentration
(MIC)
was
determined
be
150
µg/mL.
Sub-MIC
(1/8,
1/4,
1/2,
MIC)
were
evaluated
for
their
impact
inhibition
formation.
Fucoidan
treatment
resulted
in
significant,
dose-dependent
formation,
achieving
2.61
log
CFU/cm2
2.45
reductions
surfaces,
respectively.
FE-SEM
analysis
confirmed
disruption
cell
membrane
damage.
Real-time
PCR
showed
downregulation
quorum-sensing
(luxS)
virulence
(rpoS,
avrA,
hilA)
genes.
These
results
propose
that
fucoidan
has
ability
as
natural
antibacterial
agent
controlling
biofilms
processing,
thereby
enhancing
food
safety
minimizing
contamination.
Language: Английский
Prevalence, identification of virulence genes, and antibiotic resistance properties of Shiga-toxin producing Escherichia coli (STEC) strains isolated from ice cream and juice in sales centers
BMC Infectious Diseases,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: April 22, 2025
Contaminated
food
with
Shiga-toxin
producing
Escherichia
coli
(STEC)
can
cause
diarrhea
and
severe
diseases
in
consumers.
This
study
aimed
to
assess
the
phenotypic
genotypic
characteristics
of
virulence
antibiotic
resistance
genes
STEC
strains
isolated
from
various
types
ice
cream
fruit
juice
sold
Isfahan,
Iran.
From
March
2023
2024,
500
samples-including
traditional
(100),
industrial
frozen
(100)-were
collected.
Samples
were
analyzed
using
biochemical
molecular
methods
for
detection.
Antibiotic
sensitivity
was
evaluated
disc
diffusion
method
against
14
antibiotics.
Specific
primers
used
identify
genes.
Among
samples,
52
(10.42%)
E.
positive,
highest
prevalence
found
(20%)
(15%).
The
pathogenic
subtype,
particularly
enterohemorrhagic
(EHEC),
most
common
(75%
positive
samples)
(66.66%).
High
rates
observed
ampicillin
(86.53%),
tetracycline
(76.92%),
sulfamethoxazole
(73.07%),
while
lowest
recorded
imipenem
(7.69%).
frequently
detected
aadA1
tetA
(57.69%),
sul1
(55.76%).
Key
included
stx1,
stx2,
eaeA.
These
findings
emphasize
public
health
risks
associated
contamination
products
need
stricter
safety
measures
stewardship
programs.
highlight
products,
juice.
Localized
studies
are
essential
understand
specific
inform
targeted
interventions.
Strategies
such
as
improved
hygiene
practices,
stringent
regulations,
effective
programs
critical
mitigating
threat
posed
by
products.
Language: Английский
The Role of Fucoidan in Controlling Listeria monocytogenes Biofilms on Seafood-Contact Surfaces
Applied Sciences,
Journal Year:
2025,
Volume and Issue:
15(11), P. 5799 - 5799
Published: May 22, 2025
Seafood-borne
pathogens,
especially
Listeria
monocytogenes,
pose
a
significant
risk
to
global
health,
with
the
formation
of
biofilm
on
abiotic
surfaces
exacerbating
contamination
risks
in
seafood
industry.
This
investigation
evaluates
inhibition
efficacy
fucoidan
against
L.
monocytogenes
biofilms
commonly
used
processing
surfaces.
The
minimum
inhibitory
concentration
(MIC)
was
determined
be
150
µg/mL,
and
sub-MIC
concentrations
(1/8,
1/4,
1/2
MIC)
were
assessed
for
their
effects
biofilm.
action
resulted
substantial,
dose-dependent
reduction
biofilm,
maximum
reductions
2.91
log
CFU/cm2
hand
gloves
(HG),
2.46
silicone
rubber
(SR),
2.11
stainless
steel
(SS).
Gene
expression
analysis
via
real-time
quantitative
polymerase
chain
reaction
(RT-qPCR)
revealed
downregulation
quorum-sensing
(QS)
virulence-associated
genes
(flaA,
fbp,
prfA,
hlyA,
agrA),
indicating
fucoidan’s
potential
bacterial
pathogenicity.
These
results
emphasize
as
promising
environmental
antimicrobial
agent
mitigating
handling
environments,
thus
improving
food
safety
reducing
risks.
Language: Английский