The Role of Fucoidan in Controlling Listeria monocytogenes Biofilms on Seafood-Contact Surfaces DOI Creative Commons
Anamika Roy, Pantu Kumar Roy, Shin Young Park

et al.

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(11), P. 5799 - 5799

Published: May 22, 2025

Seafood-borne pathogens, especially Listeria monocytogenes, pose a significant risk to global health, with the formation of biofilm on abiotic surfaces exacerbating contamination risks in seafood industry. This investigation evaluates inhibition efficacy fucoidan against L. monocytogenes biofilms commonly used processing surfaces. The minimum inhibitory concentration (MIC) was determined be 150 µg/mL, and sub-MIC concentrations (1/8, 1/4, 1/2 MIC) were assessed for their effects biofilm. action resulted substantial, dose-dependent reduction biofilm, maximum reductions 2.91 log CFU/cm2 hand gloves (HG), 2.46 silicone rubber (SR), 2.11 stainless steel (SS). Gene expression analysis via real-time quantitative polymerase chain reaction (RT-qPCR) revealed downregulation quorum-sensing (QS) virulence-associated genes (flaA, fbp, prfA, hlyA, agrA), indicating fucoidan’s potential bacterial pathogenicity. These results emphasize as promising environmental antimicrobial agent mitigating handling environments, thus improving food safety reducing risks.

Language: Английский

Phage Endolysins as an Alternative Biocontrol Strategy for Pathogenic and Spoilage Microorganisms in the Food Industry DOI Creative Commons
Maryoris Elisa Soto Lopez, Fernando A Mendoza-Corvis, Jose Jorge Salgado-Behaine

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 564 - 564

Published: April 14, 2025

Food contamination by pathogenic and spoilage bacteria causes approximately 47 million cases of foodborne diseases in the United States leads to tons food spoilage, worsening loss situation worldwide. In addition, conventional preservation treatments implemented industry decrease food’s nutritional organoleptic quality. Therefore, there is a need for new alternatives counteract without altering its characteristics. Endolysins are promising strategy due their unique properties, such as host specificity, synergism with other antibacterial agents, mode action, low probability resistance development. These characteristics differentiate them from agents used industry. enzymes produced bacteriophages during process bacterial infection lysis. This review describes advances related endolysin application systems food, considering potential safety an overview conditions according type be controlled. We also highlight studies on encapsulation prolongation action time outbreaks that allow obtaining key information improve endolysins different matrices processing storage

Language: Английский

Citations

0

Effects of Fucoidan on the Inhibition of Biofilm Formation of Salmonella enterica Subsp. enterica Serovar Typhimurium on Seafoods and Its Molecular Antibiofilm Mechanisms DOI Creative Commons
Anamika Roy, Pantu Kumar Roy, Sung‐Rae Cho

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 914 - 914

Published: April 16, 2025

Foodborne illnesses, particularly those caused by Salmonella enterica subsp. Serovar Typhimurium, present a significant challenge to public health, especially within the seafood industry due biofilm formation on foods. This study investigated antibiofilm potential of fucoidan, sulfated polysaccharide, against Typhimurium crab and shrimp surfaces. Fucoidan’s minimum inhibitory concentration (MIC) was determined be 150 µg/mL. Sub-MIC (1/8, 1/4, 1/2, MIC) were evaluated for their impact inhibition formation. Fucoidan treatment resulted in significant, dose-dependent formation, achieving 2.61 log CFU/cm2 2.45 reductions surfaces, respectively. FE-SEM analysis confirmed disruption cell membrane damage. Real-time PCR showed downregulation quorum-sensing (luxS) virulence (rpoS, avrA, hilA) genes. These results propose that fucoidan has ability as natural antibacterial agent controlling biofilms processing, thereby enhancing food safety minimizing contamination.

Language: Английский

Citations

0

Prevalence, identification of virulence genes, and antibiotic resistance properties of Shiga-toxin producing Escherichia coli (STEC) strains isolated from ice cream and juice in sales centers DOI Creative Commons

Elyas Shahrivar,

Ebrahim Rahimi, Faham Khamesipour

et al.

BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 22, 2025

Contaminated food with Shiga-toxin producing Escherichia coli (STEC) can cause diarrhea and severe diseases in consumers. This study aimed to assess the phenotypic genotypic characteristics of virulence antibiotic resistance genes STEC strains isolated from various types ice cream fruit juice sold Isfahan, Iran. From March 2023 2024, 500 samples-including traditional (100), industrial frozen (100)-were collected. Samples were analyzed using biochemical molecular methods for detection. Antibiotic sensitivity was evaluated disc diffusion method against 14 antibiotics. Specific primers used identify genes. Among samples, 52 (10.42%) E. positive, highest prevalence found (20%) (15%). The pathogenic subtype, particularly enterohemorrhagic (EHEC), most common (75% positive samples) (66.66%). High rates observed ampicillin (86.53%), tetracycline (76.92%), sulfamethoxazole (73.07%), while lowest recorded imipenem (7.69%). frequently detected aadA1 tetA (57.69%), sul1 (55.76%). Key included stx1, stx2, eaeA. These findings emphasize public health risks associated contamination products need stricter safety measures stewardship programs. highlight products, juice. Localized studies are essential understand specific inform targeted interventions. Strategies such as improved hygiene practices, stringent regulations, effective programs critical mitigating threat posed by products.

Language: Английский

Citations

0

The Role of Fucoidan in Controlling Listeria monocytogenes Biofilms on Seafood-Contact Surfaces DOI Creative Commons
Anamika Roy, Pantu Kumar Roy, Shin Young Park

et al.

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(11), P. 5799 - 5799

Published: May 22, 2025

Seafood-borne pathogens, especially Listeria monocytogenes, pose a significant risk to global health, with the formation of biofilm on abiotic surfaces exacerbating contamination risks in seafood industry. This investigation evaluates inhibition efficacy fucoidan against L. monocytogenes biofilms commonly used processing surfaces. The minimum inhibitory concentration (MIC) was determined be 150 µg/mL, and sub-MIC concentrations (1/8, 1/4, 1/2 MIC) were assessed for their effects biofilm. action resulted substantial, dose-dependent reduction biofilm, maximum reductions 2.91 log CFU/cm2 hand gloves (HG), 2.46 silicone rubber (SR), 2.11 stainless steel (SS). Gene expression analysis via real-time quantitative polymerase chain reaction (RT-qPCR) revealed downregulation quorum-sensing (QS) virulence-associated genes (flaA, fbp, prfA, hlyA, agrA), indicating fucoidan’s potential bacterial pathogenicity. These results emphasize as promising environmental antimicrobial agent mitigating handling environments, thus improving food safety reducing risks.

Language: Английский

Citations

0