miRNAs in epilepsy: A review from molecular signatures to therapeutic intervention

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 263, P. 130468 - 130468

Published: Feb. 27, 2024

Language: Английский

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MicroRNA-mediated regulation of neurotransmitter receptors in epilepsy: A systematic review

Epilepsy & Behavior, Journal Year: 2024, Volume and Issue: 158, P. 109912 - 109912

Published: June 26, 2024

Language: Английский

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Circulating microRNAs as Biomarkers of Various Forms of Epilepsy

Medical Sciences, Journal Year: 2025, Volume and Issue: 13(1), P. 7 - 7

Published: Jan. 8, 2025

Background: Epilepsy is a group of disorders characterized by cluster clinical and EEG signs leading to the formation abnormal synchronous excitation neurons in brain. It one most common neurological worldwide; aberrant expression patterns; both at level matrix transcripts regulatory RNA sequences. Aberrant number microRNAs can mark particular epileptic syndrome; which will improve quality differential diagnosis. Materials Methods: In this work; profile six was analyzed: hsa-miR-106b-5p; hsa-miR-134-5p; hsa-miR-122-5p; hsa-miR-132-3p; hsa-miR-155-5p; hsa-miR-206-5p blood plasma patients suffering from temporal lobe epilepsy (n = 52) juvenile myoclonic 42); n-amount participants; comparison with healthy volunteers. The analysis carried out using RT-PCR. Mathematical processing data according Livak method. Results: A statistically significant change hsa-miR-132-3p found. An increase hsa-miR-134-5p hsa-miR-122-5p registered compared control; as well an hsa-miR-106b-5p control. 106b-5p; 132-3p are also able discriminate groups different syndromes. Additionally; drug-resistant drug-sensitive forms hippocampal sclerosis without Conclusion. Our allow us propose these biomarkers various

Language: Английский

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Protective mechanism of safflower yellow injection on myocardial ischemia-reperfusion injury in rats by activating NLRP3 inflammasome

BMC Complementary Medicine and Therapies, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 9, 2025

This study intended to explore whether the protective effect safflower yellow injection (SYI) on myocardial ischemia-reperfusion (I/R) injury in rats mediated of NLRP3 inflammasome signaling. The I/R model was prepared by ligating left anterior descending coronary artery for 45 min and then releasing blood flow 150 min. 96 male Wistar were randomly divided into sham group, Hebeishuang group (HBS), SYI high-dose (I/R + SYI-H), medium-dose SYI-M) low-dose SYI-L). Cell experiments normal control (NC), Oxygen glucose deprivation/reoxygenation (OGD/R), OGD/R Chloroquine (OGD/R CQ). area ischemia infarction pathological changes observed Tetrazolium method (TTC) HE staining. Myocardial enzymes such as aspartate aminotransferase (AST), lactate dehydrogenase (LDH) creatine kinase (CK) measured chemiluminescence (CL) method. inflammatory factors levels TNF-α, IL-1β, MCP-1, IL-6 detected ELISA. expressions inflammatory-related proteins (Caspase-1, NLRP3, TLR4, NF-κB), autophagosome-related (LC3-I, LC3-II,LC3-II/LC3-I), apoptosis-related (Bax, Bcl-2, Caspase-3, Bcl-2/Bax) autophagy-related (p62/SQSTM1, PI3K, p-Akt, mTOR) Western-Blot. morphology cell viability transmission electron microscopy CCK-8. In vivo, compared with percentage increased tissue arrangement disordered group. addition, activities enzymes, contents factors, proteins, Bax Caspase-3 increased, while Bcl-2 Bcl-2/Bax decreased. treatment reversed these trends, except expression proteins. vitro, decreased caused OGD/R. However, p62/SQSTM1 mTOR p-AKT, LC3-II/LC3-I significantly CQ can promote autophagy regulating thereby attenuating response protecting damaged myocardium rats.

Language: Английский

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Quantitative real-time PCR analysis of circulating MicroRNA levels in blood samples from pediatric patients with epilepsy

Gene Reports, Journal Year: 2025, Volume and Issue: unknown, P. 102214 - 102214

Published: April 1, 2025

Language: Английский

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Identification of Novel miRNA Biomarkers for Epilepsy: A qRT-PCR-Based Profiling Study

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Epilepsy is a widespread neurological disorder affecting over 70 million individuals globally, with approximately 4.9 new cases diagnosed annually. Despite extensive research, the underlying mechanisms of epilepsy remain incompletely elucidated. MicroRNAs (miRNAs) are being increasingly utilized as crucial biomarkers in various disorders, and their targeting proteins involved disease pathogenesis represents promising therapeutic strategy. This study aimed to identify novel candidate miRNAs associated evaluate diagnostic potential. The expression profiles 84 were analyzed plasma samples from 20 patients 25 controls using qRT-PCR on BioMarkTM 96.96 Dynamic Array (Fluidigm Corporation) system. In group, total 44 exhibited decreased compared control which 24 statistically significant. Notably, 18 demonstrated highly significant reduction levels (p< 0.0001). These include hsa-let-7e-5p, hsa-miR-105-5p, hsa-miR-106b-5p, hsa-miR-107, hsa-miR-125b-5p, hsa-miR-128, hsa-miR-135b-5p, hsa-miR-138-5p, hsa-miR-152, hsa-miR-15a-5p, hsa-miR-193b-3p, hsa-miR-203a, hsa-miR-339-5p, hsa-miR-381-3p, hsa-miR-409-3p, hsa-miR-511, hsa-miR-539-5p, hsa-miR-7-5p. 40 increased among 28 23 increase (p < hsa-miR-101-3p, hsa-miR-130a-3p, hsa-miR-132-3p, hsa-miR-146a-5p, hsa-miR-146b-5p, hsa-miR-181a-5p, hsa-miR-212-3p, hsa-miR-27a-3p, hsa-miR-28-5p, hsa-miR-302b-5p, hsa-miR-337-3p, hsa-miR-338-3p, hsa-miR-34a-5p, hsa-miR-455-5p, hsa-miR-485-5p, hsa-miR-487a, hsa-miR-499a-5p, hsa-miR-509-3p, hsa-miR-512-3p, hsa-miR-518b, hsa-miR-652-3p, hsa-miR-9-3p, hsa-miR-95. findings suggest that hold potential innovative, non-invasive for early detection diagnosis epilepsy, offering avenue advancing clinical practices.

Language: Английский

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Targeted miRNA Delivery in Epilepsy: Mechanisms, Advances, and Therapeutic Potential

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: April 7, 2025

Language: Английский

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The emerging role of miRNAs in epilepsy: From molecular signatures to diagnostic potential

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 254, P. 155146 - 155146

Published: Jan. 19, 2024

Language: Английский

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MiRNA Dysregulation in Brain Injury: An In Silico Study to Clarify the Role of a MiRNA Set

Current Neuropharmacology, Journal Year: 2024, Volume and Issue: 23(2), P. 209 - 231

Published: Aug. 12, 2024

The identification of specific circulating miRNAs has been proposed as a valuable tool for elucidating the pathophysiology brain damage or injury and predicting patient outcomes.

Language: Английский

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Silencing miR-155–5p alleviates hippocampal damage in kainic acid-induced epileptic rats via the Dusp14/MAPK pathway

Brain Research Bulletin, Journal Year: 2024, Volume and Issue: 217, P. 111057 - 111057

Published: Aug. 28, 2024

Epilepsy with recurrent seizures is characterized by neuronal damage and glial proliferation induced brain inflammation. Recurrent can lead to changes in the microRNA (miRNA) spectrum, significantly influencing inflammatory response of microglia. MiR-155-5p, as a pro-inflammatory miRNA, increased epileptic brain. However, its specific role acute remains unknown. The study aimed develop new strategy for treating epilepsy investigating how silencing miR-155-5p initiated anticonvulsive mechanism. level was up-regulated hippocampus immature rats kainic acid (KA). use antago-miR-155-5p exerted significant beneficial effects on seizure scores, discharges cognition following KA-induced epilepsy. Antago-miR-155-5p also inhibited neuron microglial activation. Moreover, Dual-specificity phosphatase 14 (Dusp14)/ mitogen-activated protein kinase (MAPK) axis vivo. MiR-155-5p interacted dusp14 regulate MAPK signaling way expression, verified dual-luciferase reporter assay. results suggested that might reduce hippocampal KA via Dusp14/MAPK way. This implied could serve therapeutic tool prevent development

Language: Английский

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