Frontiers in Microbiology,
Journal Year:
2019,
Volume and Issue:
10
Published: Aug. 23, 2019
The
Asian
citrus
psyllid
(Diaphorina
citri)
is
a
major
pest
of
trees
as
it
transmits
Candidatus
Liberibacter
asiaticus
(CLas).
composition
host's
microbiota
can
affect
the
evolution
and
ecological
distribution
host.
This
study
monitored
compositional
shifts
in
through
all
life
stages
(egg,
nymph
1–5
stages,
adult)
by
next-generation
sequencing
quantitative
real-time
PCR.
There
were
clear
differences
both
α-
β-diversity
stages.
Microbiota
diversity
was
markedly
higher
2–5
than
adult,
egg,
1
Proteobacteria
dominant
D.
citri,
representing
>
97.5%
total
bacterial
community,
Profftella
armature
genus
Data
from
qPCR
analysis
showed
an
exponential
increase
populations
three
citri
endosymbionts:
armature,
Carsonella
ruddii,
Wolbachia.
gut
bacterium
Pantoea
present
but
2-5
substantially
differed
among
egg–nymph
1,
nymphs
2–5,
adult
Therefore,
we
successfully
characterized
dynamics
thus
identified
shift
during
cycle
16S
rRNA
gene
Moreover,
suggested
that
acquired
ability
to
bear
CLas
at
stage.
enhances
our
understanding
microbial
establishment
developing
provides
reference
resource
for
identification
potential
biocontrol
approaches
against
this
pest.
Journal of Pest Science,
Journal Year:
2018,
Volume and Issue:
91(4), P. 1251 - 1267
Published: June 22, 2018
Drosophila
suzukii,
commonly
known
as
the
spotted
wing
drosophila
(SWD),
is
an
exotic
fruit
fly
from
Southeast
Asia
that
was
introduced
to
temperate
regions
of
North
America
and
Europe
in
2008.
It
attacks
a
wide
variety
fruits
has
become
devastating
pest
soft-skinned
crops.
Due
rapid
spread
SWD
across
newly
invaded
continents,
fresh
markets
have
zero-tolerance
policy
regarding
D.
suzukii
infestation.
Specific
efficient
detection
tools
are
urgently
needed
so
farmers
can
deliver
timely
management
interventions
meet
market
demands.
Since
be
attracted
damaged
rotting
fruits,
headspace
volatiles
fermented
apple
juices
were
collected
analyzed
by
gas
chromatography-mass
spectrometry.
Special
attention
given
compounds
produced
and/or
enriched
during
fermentation
process.
After
performing
series
laboataory
field
tests,
we
identified
quinary
blend,
which
more
selective
for
than
currently
standard
cider
vinegar
commercially
available
lure
under
conditions.
Identification
attractant
will
help
growers
accurately
detect
adult
infestations
orchards,
thereby
allowing
while
reducing
conventional
insecticidal
usage
protect
our
crops,
environment,
ecosystem.
Journal of Applied Entomology,
Journal Year:
2017,
Volume and Issue:
142(1-2), P. 18 - 25
Published: June 23, 2017
Abstract
The
invasiveness
of
Drosophila
suzukii
Matsumura
is
causing
sizable
damage
to
soft
fruits
and
cherry.
In
order
define
a
targeted
strategy
for
D.
management,
it
important
have
access
highly
sensitive
trapping
tools
detection,
monitoring
control
that
are
also
selective,
practical
in
use,
economic,
with
low
environmental
impact.
aim
this
study
was
compare
different
combinations
traps
lures
best
practice
approach
Italy,
extended
over
wide
elevational
gradient
landscape
complexity.
high
attractiveness
not
always
combined
selectivity,
as
some
cases
up
95%
the
spp.
captured
were
belonging
species
from
.
commercial
Droso‐Trap
very
efficient
providing
capture
ability.
Overall,
most
attractive
lure
Droskidrink
while
Suzukii
Trap
selective
one.
Lure
however,
changed
during
season
relation
temperatures
phenological
developmental
stages,
suggesting
need
implementing
periods
purposes
(monitoring
or
mass
trapping).
terms
trap
showed
problem
bacterial‐gel
formation,
Pherocon
SWD
easier
handle
they
did
be
checked
at
weekly
interval.
Finally,
these
results
gathered
convey
combination
bait
able
efficiently
perform
attract
kill
technique.
Frontiers in Microbiology,
Journal Year:
2019,
Volume and Issue:
10
Published: Aug. 23, 2019
The
Asian
citrus
psyllid
(Diaphorina
citri)
is
a
major
pest
of
trees
as
it
transmits
Candidatus
Liberibacter
asiaticus
(CLas).
composition
host's
microbiota
can
affect
the
evolution
and
ecological
distribution
host.
This
study
monitored
compositional
shifts
in
through
all
life
stages
(egg,
nymph
1–5
stages,
adult)
by
next-generation
sequencing
quantitative
real-time
PCR.
There
were
clear
differences
both
α-
β-diversity
stages.
Microbiota
diversity
was
markedly
higher
2–5
than
adult,
egg,
1
Proteobacteria
dominant
D.
citri,
representing
>
97.5%
total
bacterial
community,
Profftella
armature
genus
Data
from
qPCR
analysis
showed
an
exponential
increase
populations
three
citri
endosymbionts:
armature,
Carsonella
ruddii,
Wolbachia.
gut
bacterium
Pantoea
present
but
2-5
substantially
differed
among
egg–nymph
1,
nymphs
2–5,
adult
Therefore,
we
successfully
characterized
dynamics
thus
identified
shift
during
cycle
16S
rRNA
gene
Moreover,
suggested
that
acquired
ability
to
bear
CLas
at
stage.
enhances
our
understanding
microbial
establishment
developing
provides
reference
resource
for
identification
potential
biocontrol
approaches
against
this
pest.
