The microbiome of pest insects: it is not just bacteria

Entomologia Experimentalis et Applicata, Journal Year: 2019, Volume and Issue: 167(3), P. 156 - 170

Published: Feb. 27, 2019

Abstract Insects are associated with multiple microbes that have been reported to influence various aspects of their biology. Most studies in insects, including pest species, focus on the bacterial communities microbiome even though consists members many more kingdoms, which can also large life history insects. In this review, we present some key examples how different microbiome, such as bacteria, fungi, viruses, archaea, and protozoa, affect fitness behavior Moreover, argue interactions within among microbial groups abundant great importance, necessitating use a community approach study microbial–host interactions. We propose restricted bacteria very likely hampers our understanding functioning impact biology close review by highlighting few open questions provide an in‐depth other components addition might host performance, thus contributing insect ecology.

Language: Английский

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Near full-length 16S rRNA gene next-generation sequencing revealed Asaia as a common midgut bacterium of wild and domesticated Queensland fruit fly larvae

Microbiome, Journal Year: 2018, Volume and Issue: 6(1)

Published: May 5, 2018

Gut microbiota affects tephritid (Diptera: Tephritidae) fruit fly development, physiology, behavior, and thus the quality of flies mass-reared for sterile insect technique (SIT), a target-specific, sustainable, environmentally benign form pest management. The Queensland fly, Bactrocera tryoni (Tephritidae), is significant horticultural in Australia can be managed with SIT. Little known about impacts that laboratory-adaptation (domestication) mass-rearing have on larval gut microbiome. Read lengths previous next-generation sequencing (NGS) studies limited resolution microbiome studies, diversity within populations often overlooked. In this study, we used new near full-length (> 1300 nt) 16S rRNA gene amplicon NGS approach to characterize bacterial communities individual B. larvae from two field (developing peaches) three domesticated (mass- or laboratory-reared artificial diets). Near sequences were obtained 56 larvae. OTU clustering at 99% similarity revealed was low significantly lower Bacteria commonly associated (Acetobacteraceae, Enterobacteriaceae, Leuconostocaceae) detected wild larvae, but largely absent However, Asaia, an acetic acid bacterium not frequently adult species, both (55 out samples). Larvae same single peach shared similar profile, whereas different peaches collected tree had profiles. Clustering Asaia 100% showed locations strains. Variation depends diet, domestication, horizontal acquisition. Bacterial variation suggests more than one species perform functional role; however, could important warrants further study. A greater understanding functions bacteria lead increased performance as part

Language: Английский

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Tephritid-microbial interactions to enhance fruit fly performance in sterile insect technique programs

BMC Microbiology, Journal Year: 2019, Volume and Issue: 19(S1)

Published: Dec. 1, 2019

Abstract Background The Sterile Insect Technique (SIT) is being applied for the management of economically important pest fruit flies (Diptera: Tephritidae) in a number countries worldwide. success and cost effectiveness SIT depends upon ability mass-reared sterilized male insects to successfully copulate with conspecific wild fertile females when released field. Methods We conducted critical analysis literature about tephritid gut microbiome including advancement methods identification characterization microbiota, particularly next generation sequencing, impacts irradiation (to induce sterility flies) fly rearing, use probiotics manipulate microbiota. Results Domestication, mass-rearing, handling, as required SIT, may change structure flies’ microbial community compared that under field conditions. Gut microbiota tephritids are their hosts’ development, performance physiology. Knowledge how mass-rearing associated changes impact functional role bacteria host biology limited. Probiotics offer potential encourage limits pathogens, improves quality flies. Conclusions Advances technologies used identify characterize will continue expand our understanding diversity composition. functions microbes increase through gnotobiotic models, genome metagenomics, metatranscriptomics, metabolomics metaproteomics. probiotics, or manipulation offers significant opportunities enhance production high quality, performing operational programs.

Language: Английский

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Specialized acquisition behaviors maintain reliable environmental transmission in an insect-microbial mutualism

Current Biology, Journal Year: 2023, Volume and Issue: 33(13), P. 2830 - 2838.e4

Published: June 28, 2023

Language: Английский

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Chemical and Electrophysiological Characterisation of Headspace Volatiles from Yeasts Attractive to Drosophila suzukii

Journal of Chemical Ecology, Journal Year: 2024, Volume and Issue: 50(11), P. 830 - 846

Published: May 1, 2024

Abstract Chemical control of Drosophila suzukii (Diptera: Drosophilidae) based on the use insecticides is particularly challenging as insect attacks ripening fruits shortly before harvest. An alternative strategy may rely yeasts phagostimulants and baits, applied canopy attract-and-kill formulations. The aim this research was to identify most attractive among six yeast species for D. : Saccharomyces cerevisiae , Hanseniaspora uvarum Clavispora santaluciae Saccharomycopsis vini Issatchenkia terricola Metschnikowia pulcherrima . volatile profile C described first time. Behavioural experiments identified H. S. yeasts. characterization headspace volatiles using direct (DHS) solid-phase microextraction (SPME) revealed several strain-specific compounds. With DHS injection, 19 were characterised, while SPME 71 compounds constituting headspace. Both analyses terpenoids including β-ocimene, citronellol, ( Z )-geraniol (nerol), geranial distinct constituents further investigated closed-loop stripping analysis (CSLA) electroantennography. Out 14 quantified by CSLA, ethyl acetate, isoamyl β-myrcene, benzaldehyde linalool detected antennae might generate strong attractiveness uvarum. Our results highlight a attraction various associated with both flies their habitat demonstrate how different sampling methods can impact compound characterization. It remains be demonstrated whether special adaptations certain what extent metabolites causing are interchangeable.

Language: Английский

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Ceratitis capitata microbiota and its effect on environmental stress tolerance: making flies stronger

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Abstract Ceratitis capitata (Wiedemann) is a cosmopolitan pest of economic importance. It controlled by using the Sterile Insect Technique (SIT), which involves rearing and release sterile males destined to mate with wild females, causing generation‐to‐generation suppression. Medflies are colonized microorganisms, primarily Enterobacteriaceae, genera Klebsiella Enterobacter being most common. Such microbiota contributes host fitness. During SIT, diet antibiotics irradiation for sterility adults alter microbiota. We aimed determine role Medfly on resistance abiotic stress conditions, evaluating its function under: (i) starvation, (ii) elevated temperatures, (iii) dry environments. These conditions simulate challenges may encounter after release, differ from compared adult survival between symbiotic aposymbiotic individuals, under two thermal regimes (25 30 °C) or humidity (20%–25% 80%–90% R.H.). Aposymbiotic individuals were obtained providing them water containing mixture methylparaben. Treatment antimicrobials effectively reduced gut While starvation had no significant effect survival, higher proportion died earlier at °C humidity, more pronounced 48 h. Our results suggest that plays in adaptation environmental stress. report presence culturable yeast digestive tract C. , Zygosaccharomyces rouxii . Providing probiotic bacteria Z. prior could improve SIT outcomes adverse conditions.

Language: Английский

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Microbiome of the Queensland Fruit Fly through Metamorphosis

Microorganisms, Journal Year: 2020, Volume and Issue: 8(6), P. 795 - 795

Published: May 26, 2020

Bactroceratryoni (Froggatt) (Queensland fruit fly, or "Qfly") is a highly polyphagous tephritid fly and serious economic pest in Australia. Qfly biology intimately linked to the bacteria fungi of its microbiome. While there are numerous studies microbiome larvae adults, transition through pupal stage remains unknown. To address this knowledge gap, we used high-throughput Next-Generation Sequencing (NGS) examine microbial communities at each developmental life cycle, targeting bacterial 16S rRNA fungal ITS regions. We found that were similar larval also between adult males females, yet marked differences stages. Specific taxa present adults (fed hydrolyzed yeast with sugar) which likely related nutritional these observed significant abundance Acetobacteraceae family level, both Conversely, Enterobacteriaceae was abundant (> 80%) only adults. The majority yeasts yeast-like fungi. In addition elucidating changes stages, study characterizes establishment laboratory colonies as they enter domestication process.

Language: Английский

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The vectoring of Starmerella species and other yeasts by stingless bees in a Neotropical savanna

Fungal ecology, Journal Year: 2020, Volume and Issue: 47, P. 100973 - 100973

Published: July 10, 2020

Language: Английский

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Live yeast in juvenile diet induces species-specific effects on Drosophila adult behaviour and fitness

Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)

Published: June 20, 2019

Abstract The presence and the amount of specific yeasts in diet saprophagous insects such as Drosophila can affect their development fitness. However, impact different yeast species juvenile has rarely been investigated. Here, we measured behavioural fitness effects three live ( Saccharomyces cerevisiae = SC; Hanseniaspora uvarum HU; Metschnikowia pulcherrima MP) added to melanogaster larvae. Beside these naturally found natural populations or food sources, tested inactivated “drySC” widely used research laboratories. All flies were transferred drySC medium immediately after adult emergence, several life traits behaviours measured. These four diets had on pre-imaginal development: HU-rich tended shorten “egg-to-pupa” period while MP-rich induced higher larval lethality compared other diets. Pre- postzygotic reproduction-related characters (copulatory ability, fecundity, cuticular pheromones) varied according sex. Juvenile also changed choice preference longevity. results indicate that present sources ingested by larvae crucial for

Language: Английский

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Fungal Microbiota of Sea Buckthorn Berries at Two Ripening Stages and Volatile Profiling of Potential Biocontrol Yeasts

Microorganisms, Journal Year: 2020, Volume and Issue: 8(3), P. 456 - 456

Published: March 23, 2020

Sea buckthorn, Hippophae rhamnoides L., has considerable potential for landscape reclamation, food, medicinal, and cosmetics industries. In this study, we analyzed fungal microorganism populations associated with carposphere of sea buckthorn harvested in Lithuania. An amplicon metagenomic approach based on the ITS2 region rDNA was used to reveal ripening-affected community alterations berries. According alpha beta diversity analyses, depending ripening stage, displayed significantly different communities. Unripe berries were shown be prevalent by Aureobasidium, Taphrina, Cladosporium, while ripe dominated Aureobasidium Metschnikowia. The selected yeast strains from unripe mature applied volatile organic compounds identification gas chromatography mass spectrometry techniques. It demonstrated that patterns volatiles four species tested distinct each other. current study first time revealed communities colonizing surface at stages. novel information specific profiles cultivable buckthorn-associated yeasts a role biocontrol is important development strategies plant cultivation disease management, as well improvement quality preservation postharvest Management microorganisms present might powerful instrument control phytopathogenic potentially antagonistic affecting

Language: Английский

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