Prospects and challenges of implementing DNA metabarcoding for high-throughput insect surveillance

GigaScience, Journal Year: 2019, Volume and Issue: 8(8)

Published: July 30, 2019

Abstract Trap-based surveillance strategies are widely used for monitoring of invasive insect species, aiming to detect newly arrived exotic taxa as well track the population levels established or endemic pests. Where these traps have low specificity and capture non-target species in excess target pests, need extensive specimen sorting identification creates a major diagnostic bottleneck. While recent development standardized molecular diagnostics has partly alleviated this requirement, single per reaction nature methods does not readily scale sheer number insects trapped programmes. Consequently, lists often restricted few high-priority allowing unanticipated avoid detection potentially establish populations. DNA metabarcoding recently emerged method conducting simultaneous, multi-species complex mixed communities may lend itself ideally rapid bulk trap samples. Moreover, high-throughput sequencing platforms could enable multiplexing hundreds diverse samples on flow cell, thereby providing means dramatically up terms both quantity that can be processed concurrently pest targeted. In review literature, we explore how tailored context highlight unique technical regulatory challenges must considered when implementing technologies into sensitive applications.

Language: Английский

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Conservation and the Genomics of Populations

Oxford University Press eBooks, Journal Year: 2022, Volume and Issue: unknown

Published: Feb. 10, 2022

Abstract Loss of biodiversity is among the greatest problems facing world today. Conservation and Genomics Populations gives a comprehensive overview essential background, concepts, tools needed to understand how genetic information can be used conserve species threatened with extinction, manage ecological or commercial importance. New molecular techniques, statistical methods, computer programs, principles, methods are becoming increasingly useful in conservation biological diversity. Using balance data theory, coupled basic applied research examples, this book examines phenotypic variation natural populations, principles mechanisms evolutionary change, interpretation from these conservation. The includes examples plants, animals, microbes wild captive populations. This third edition has been thoroughly revised include advances genomics contains new chapters on population genomics, monitoring, genetics practice, as well sections climate emerging diseases, metagenomics, more. More than one-third references were published after previous edition. Each 24 Appendix end Guest Box written by an expert who provides example presented chapter their own work. for advanced undergraduate graduate students genetics, resource management, biology, professional biologists policy-makers working wildlife habitat management agencies. Much will also interest nonprofessionals curious about role

Language: Английский

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Insights into insecticide-resistance mechanisms in invasive species: Challenges and control strategies

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 9, 2023

Threatening the global community is a wide variety of potential threats, most notably invasive pest species. Invasive species are non-native organisms that humans have either accidentally or intentionally spread to new regions. One effective and first lines control strategies for controlling pests application insecticides. These toxic chemicals employed get rid pests, but they pose great risks people, animals, plants. Pesticides heavily used in managing current era. Due overuse synthetic chemicals, numerous already developed resistance. The resistance development main reason failure manage Developing pesticide management techniques necessitates thorough understanding mechanisms through which insects acquire insecticide Insects use behavioral, biochemical, physiological, genetic, metabolic methods deal with can lead continuous overexpression detoxifying enzymes. An overabundance enzymes causes resistance, pesticides rendering them ineffective against pests. A key factor amplification certain enzymes, specifically esterases, Glutathione S-transferase, Cytochromes p450 monooxygenase, hydrolyses. Additionally, insect guts offer unique habitats microbial colonization, gut bacteria may serve their hosts useful services. Most importantly, detoxification insecticides leads development. complete knowledge could be very helpful coping challenges effectively developing Integrated Pest Management particularly at lowering risk chemical environmental contaminants resulting health issues, it also ways

Language: Английский

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Technology innovation: advancing capacities for the early detection of and rapid response to invasive species

Biological Invasions, Journal Year: 2019, Volume and Issue: 22(1), P. 75 - 100

Published: Dec. 31, 2019

Abstract The 2016 – 2018 National Invasive Species Council ( NISC ) Management Plan and Executive Order 13751 call for US federal agencies to foster technology development application address invasive species their impacts. This paper complements draws on an Innovation Summit, review of advanced biotechnologies applicable management, a survey that respond these high-level directives. We provide assessment government capacities the early detection rapid response (EDRR) through advances in application; examples emerging technologies detection, identification, reporting, species; guidance fostering further advancements technologies. Throughout paper, we how are applying improve programmatic effectiveness cost-efficiencies. also highlight outstanding technology-related needs identified by overcome barriers enacting EDRR. Examples include improvements research facility infrastructure, data mobilization across wide range parameters (from genetic landscape scales), promotion support filling key gaps technological capacity (e.g., portable, field-ready devices with automated capacities), greater investments prizes challenge competitions.

Language: Английский

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Genomic biosurveillance of forest invasive alien enemies: A story written in code

Evolutionary Applications, Journal Year: 2019, Volume and Issue: 13(1), P. 95 - 115

Published: Aug. 12, 2019

Abstract The world's forests face unprecedented threats from invasive insects and pathogens that can cause large irreversible damage to the ecosystems. This threatens capacity provide long‐term fiber supply ecosystem services range carbon storage, nutrient cycling, water air purification, soil preservation maintenance of wildlife habitat. Reducing threat forest alien species requires vigilant biosurveillance, process gathering, integrating, interpreting, communicating essential information about pest pathogen achieve early detection warning enable better decision‐making. is challenging due diversity pests need be identified, diverse pathways introduction, difficulty in assessing risk establishment. Genomics powerful new solutions biosurveillance. invasion a story written four chapters: transport, establishment, spread. series processes lead successful leave behind DNA signature tells an invasion. help us understand dynamic, multistep inform management current future introductions. review describes application genomic tools pipelines will accurate identification pathogens, assign outbreak or survey samples putative sources identify spread, assess based on traits impact outcome.

Language: Английский

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Invasive Insect Species: Global Challenges, Strategies & Opportunities

Frontiers in Insect Science, Journal Year: 2021, Volume and Issue: 1

Published: April 8, 2021

SPECIALTY GRAND CHALLENGE article Front. Insect Sci., 08 April 2021 | https://doi.org/10.3389/finsc.2021.650520

Language: Английский

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Forest Insect Biosecurity: Processes, Patterns, Predictions, Pitfalls

Annual Review of Entomology, Journal Year: 2022, Volume and Issue: 68(1), P. 211 - 229

Published: Oct. 6, 2022

The economic and environmental threats posed by non-native forest insects are ever increasing with the continuing globalization of trade travel; thus, need for mitigation through effective biosecurity is greater than ever. However, despite decades research implementation preborder, border, postborder preventative measures, insect invasions continue to occur, no evidence saturation, even predicted accelerate. In this article, we review measures used mitigate arrival, establishment, spread, impacts possible impediments successful these measures. Biosecurity successes likely under-recognized because they difficult detect quantify, whereas failures more evident in continued establishment additional species. There limitations existing systems at global country scales (for example, inspecting all imports impossible, phytosanitary perfect, knownunknowns cannot be regulated against, noncompliance an ongoing problem). should a shared responsibility across countries, governments, stakeholders, individuals.

Language: Английский

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Genomic investigations of successful invasions: the picture emerging from recent studies

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 16, 2025

ABSTRACT Invasion biology aims to identify traits and mechanisms that contribute successful invasions, while also providing general insights into the underlying population expansion adaptation rapid climate habitat changes. Certain phenotypic attributes have been linked role of genetics has critical in understanding invasive species. Nevertheless, a comprehensive summary evaluating most common evolutionary associated with invasions across species environments is still lacking. Here we present systematic review studies since 2015 applied genomic tools investigate different organisms. We examine demographic patterns such as changes diversity at level, presence genetic bottlenecks gene flow range. selection from standing variation de novo mutations, hybridisation introgression, all which can an impact on invasion success. This recent articles led creation searchable database provide researchers accessible resource. Analysis this allowed quantitative assessment adaptive acting A predominant admixture increasing levels enabling molecular novel habitats important finding our study. The “genetic paradox” was not validated data ecosystems. Even though drift commonly reported upon invasion, large reduction rarely observed. Any decrease often relatively mild almost always restored via between populations. fact loci under are frequently detected suggests level hindered. above findings confirmed herein for first time semi‐quantitative manner by data. point gaps potential improvements design driving These include scarcity sampling multiple native populations, identification sources, longitudinal sampling, integration fitness measures analyses. note whole genome exploited fully predicting potential. Comparative identifying features promoting underrepresented despite their use tool control.

Language: Английский

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A framework to integrate innovations in invasion science for proactive management

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2022, Volume and Issue: 97(4), P. 1712 - 1735

Published: April 22, 2022

ABSTRACT Invasive alien species (IAS) are a rising threat to biodiversity, national security, and regional economies, with impacts in the hundreds of billions U.S. dollars annually. Proactive or predictive approaches guided by scientific knowledge essential keeping pace growing invasions under climate change. Although rapid development diverse technologies has produced tools potential greatly accelerate invasion research management, innovation far outpaced implementation coordination. Technological methodological syntheses urgently needed close gap facilitate interdisciplinary collaboration synergy among evolving disciplines. A broad review is necessary demonstrate utility relevance work fields generate actionable science for ongoing crisis. Here, we such advances relevant including remote sensing, epidemiology, big data analytics, environmental DNA (eDNA) sampling, genomics, others, present generalized framework distilling existing emerging into products proactive IAS management. This integrated workflow provides pathway scientists practitioners disciplines contribute applied biology coordinated, synergistic, scalable manner.

Language: Английский

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Biosurveillance for invasive insect pest species using an environmental DNA metabarcoding approach and a high salt trap collection fluid

Ecology and Evolution, Journal Year: 2021, Volume and Issue: 11(4), P. 1558 - 1569

Published: Jan. 28, 2021

With the increase in global trade and warming patterns, movement, introduction, establishment of non-native insect species has increased. A rapid effective early detection biosurveillance program to identify concern is needed reduce future impacts costs associated with introduced species. One challenges facing surveillance trapping methods sheer volume individual specimens collections. Although molecular identification are improving, they currently have limitations (e.g., destructive processing specimens) a protocol addressing these can support regulatory applications that need morphological evidence corroborate data.The novel presented here uses metabarcoding approach amplify environmental DNA from saturated salt solution trap fluid, which retains for downstream identifications. The use preserve traps addresses issues high evaporation rate ethanol traps, public safety concerns other fluid preservation options unattended settings.Using approach, 407-nucleotide segment cytochrome

Language: Английский

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