Genome-Wide Scanning Loci and Differentially Expressed Gene Analysis Unveils the Molecular Mechanism of Chlorantraniliprole Resistance in Spodoptera frugiperda DOI
Zhongxiang Sun, Zhihui Lu,

Tianxiang Xiao

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(38), P. 14092 - 14107

Published: Sept. 12, 2023

Chlorantraniliprole has been widely used to controlSpodoptera frugiperda, but it led the development of chlorantraniliprole resistance. Multiomics analysis strains with two extreme traits helps elucidate complex mechanisms involved. Herein, following genome resequencing and application Euclidean distance algorithm, 550 genes within a 16.20-Mb-linked region were identified from chlorantraniliprole-resistant (Ch-R) chlorantraniliprole-susceptible (Ch-Sus) strains. Using transcriptome sequencing, 2066 differentially expressed between Ch-R Ch-Sus Through association analysis, three glutathione S-transferase family four trehalose transporter selected for functional verification. Notably, SfGSTD1 had strongest binding ability is responsible tolerance. The strain also increased intracellular content by upregulating transcription SfTret1, thereby contributing These findings provide new perspective reveal mechanism resistance agricultural pests insecticides.

Language: Английский

Self-assembled co-delivery nanoplatform for increasing the broad-spectrum susceptibility of fall armyworm toward insecticides DOI Creative Commons
Shuo Yan, Mingjian Li, Qinhong Jiang

et al.

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Unscientific application of insecticides has led to severe resistance pests almost all classes insecticides. Enhanced detoxification is the most common mechanism for this kind resistance. Object: Fall armyworm (FAW) developed insecticide resistance, which often linked overexpression genes. Herein, a multicomponent nano-pesticide designed increase its broad-spectrum susceptibility toward Regulatory function nuclear factor erythroid 2-related 2 (Nrf2) in was confirmed using transcriptome sequencing, quantitative real-time PCR and enzyme activity measurement. A star polycation (SPc) adopted construct pesticide/SPc/complex, whose self-assembly characterization were examined isothermal titration calorimetry, dynamic light scattering transmission electron microscope. The delivery efficiency SPc-loaded dsRNA vitro vivo fluorescent tracer technique. created through integration bacterial expression system nano-delivery system, bioactivity tested laboratory field. We crucial role Nrf2 regulating FAW, silencing could decrease gene susceptibility. then applied SPc self-assemble nanoplatform delivering double-stranded RNA (dsRNA) pesticide simultaneously. Nano-sized pesticide/SPc/dsRNA complex exhibited high vivo. Excitingly, insecticidal activities pesticide/SPc/dsNrf2 complexes remarkably improved with normalized synergistic ratios 5.43-6.25 chlorantraniliprole, 4.45-15.00 emamectin benzoate, 6.75-15.00 spinetoram. Finally, we (pesticide/SPc/dsNrf2 complex) system. This approach excellent leaf protection pest control efficacy. between nanometerization improvement offers promising strategy activity. Our study provides revolutionary universal decease doses.

Language: Английский

Citations

17
Resistance monitoring and mechanism in the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) for chlorantraniliprole from Sichuan Province, China DOI Creative Commons
Huilin Chen, Ali Hasnain,

Qinghua Cheng

et al.

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: May 5, 2023

The fall armyworm, Spodoptera frugiperda (Noctuidae: Lepidoptera), is a wide-reaching notorious insect pest of important cereal crops, which has developed resistance to multiple classes insecticides. It invaded the Sichuan Province China in 2019. In this study, we performed monitoring insecticides for 11 field-collected populations from Sichuan, and all were susceptible emamectin benzoate chlorpyrifos. variations level indoxacarb (resistance ratio (RR), 9.23-45.53-fold), spinetoram (RR, 4.32-18.05-fold), chlorantraniliprole 2.02-10.39-fold) observed among these populations. To investigate mechanism chlorantraniliprole, synergism tests showed that piperonyl butoxide had slight synergistic effect on QJ-20 population (1.43-fold) moderate 10.39-fold) compared with treatment group without synergist. Furthermore, expression scanning resistance-related genes five P450 (CYP6AE43, CYP321A8, CYP305A1, CYP49A1, CYP306A1) ryanodine receptor gene (Ryr, target) overexpressed population. These results indicated armyworm exhibited diverse susceptibilities several insecticides, overexpression Ryr may contribute development S. chlorantraniliprole.

Language: Английский

Citations

28
Two cuticle-enriched chemosensory proteins confer multi-insecticide resistance in Spodoptera frugiperda DOI
Huanhuan Wang, Rui Zhao, Jie Gao

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 266, P. 130941 - 130941

Published: March 22, 2024

Language: Английский

Citations

9
Differential detoxification enzyme profiles in C-corn strain and R-rice strain of Spodoptera frugiperda by comparative genomic analysis: insights into host adaptation DOI Creative Commons
Pengfei Yao,

Syed Husne Mobarak,

Mao‐Fa Yang

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 6, 2025

The fall armyworm (FAW) Spodoptera frugiperda, a highly invasive, polyphagous pest, poses global agricultural threat. It has two strains, the C-corn and R-rice each with distinct host preferences. This study compares detoxification enzyme gene families across these strains related species to explore their adaptation diverse plant metabolites. A total of 1,995 detoxification-related genes, including cytochrome P450 monooxygenases (CYPs), carboxylesterases (COEs), glutathione S-transferases (GSTs), UDP-glucuronosyltransferases (UGTs), ATP-binding cassette transporters (ABCs), were identified genus Spodoptera, S. littoralis, litura, picta, exigua, both FAW strains. higher abundance phase I enzymes (CYPs COEs) GSTs was observed in species, while exhibited fewer notable differences copy numbers between C R Analyses at subfamily level revealed significant variation distribution expression, particularly within II enzymes. Expansions CYP6AE detected strain, contractions GST-ε, CYP9A, CYP4M, UGT33B, UGT33F occurred In contrast, no substantial III ABC Functional predictions protein interaction networks suggest broader expansion metabolism-related genes strain compared strain. These findings emphasize pivotal role adaptation, providing molecular insights into FAW's capacity for range expansion, which are crucial devising targeted sustainable pest management strategies. differential expression different frugiperda.

Language: Английский

Citations

1
Regulatory influence of Cotesia ruficrus on the development and nutrient metabolism of Spodoptera frugiperda DOI
Xian Li,

Jun‐Long An,

Na Qiao

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

Abstract BACKGROUND The fall armyworm (FAW), Spodoptera frugiperda is a notorious invasive pest that poses serious threat to global agricultural production and food security. In China, Cotesia ruficrus has emerged as promising indigenous biological control agent for controlling FAW populations. this study, we conducted series of comprehensive experiments explore the effects C. parasitization on development nutrient metabolism larvae. RESULTS Parasitization by significantly inhibited larvae, reduced their intake more than 60%. Furthermore, concentrations carbohydrate triglyceride increased from third fifth day seventh after parasitization, respectively. However, both levels subsequently decreased during later stages. Transcriptome analysis showed there were 2096 differentially expressed genes (DEGs) between parasitized non‐parasitized These DEGs found be primarily enriched in pathways related immune response, detoxification, lipid synthesis metabolism, protein metabolism. CONCLUSION Taken together, these results strongly indicate ability modulate ultimately benefiting successful parasitoid offspring. findings contribute our understanding intricate interactions host insects natural enemies provide valuable insights into sustainable effective strategies control. © 2025 Society Chemical Industry.

Language: Английский

Citations

1
Investigation of lambda-cyhalothrin resistance in Spodoptera frugiperda: Heritability, cross-resistance, and mechanisms DOI

Zhimin Guo,

Jiahui Tang,

Huina Ma

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 202, P. 105916 - 105916

Published: April 20, 2024

Language: Английский

Citations

7
Risk assessment, fitness cost and transcriptome analysis of cyantraniliprole resistance in Spodoptera frugiperda DOI
Jiaping Wei,

Shushu Lv,

Daojie Guan

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 203, P. 106000 - 106000

Published: June 21, 2024

Language: Английский

Citations

6
Monitoring and Detection of Insecticide Resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae): Evidence for Field-Evolved Resistance in Egypt DOI Creative Commons
Moataz A. M. Moustafa,

Nourhan A. El-Said,

Nawal Abdulaziz Alfuhaid

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(9), P. 705 - 705

Published: Sept. 16, 2024

Spodoptera frugiperda (J.E. Smith) (Noctuidae: Lepidoptera) is a notable insect pest that invades major cereal crops, causing significant damage and loss. Resistances of 2nd instar larvae two Egyptian field populations S. frugiperda, collected from the Fayoum Giza governments, were measured against eight insecticides, including traditional insecticides (profenofos cypermethrin), bio-insecticides (emamectin benzoate, spinosad, Bacillus thuringiensis), growth regulators (IGRs) (lufenuron, diflubenzuron, methoxyfenozide). In addition, synergistic effects three synergists (Piperonyl butoxide (PBO), diethyl maleate (DEM), triphenyl phosphate (TPP) assessed, activities detoxification enzymes (acetylcholine esterase (AChE), cytochrome P-450 (CYP-450), carboxylesterase (CarE), glutathione-s-transferase (GST) also determined. Resistance surveillance revealed population showed moderate resistance to cypermethrin (RR = 5.75-fold), followed by spinosad 2.62-fold), lufenuron (2.01-fold). On other hand, exhibited only 3.65-fold). Our results emamectin benzoate was most effective insecticide, with an LC50 value 0.003 mg/L for 0.001 population, compared susceptible strain (0.005 mg/L). Among biological thuringiensis least toxic insecticide all tested strains. Synergism assays indicated DEM TPP had effect on (SR 8.00-fold both), PBO 5.71-fold) alone. The assay GST activity significantly (p < 0.05) increased in strains strain. However, no changes observed among CYP-450, CarE, or AChE. findings this study provide substantial insights into tracking managing development Egypt.

Language: Английский

Citations

6
Characterization of the physiological, histopathological, and gene expression alterations in Spodoptera frugiperda larval midguts affected by toosendanin exposure DOI

Yanzheng Lin,

Yuting Huang, Jiafu Liu

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 195, P. 105537 - 105537

Published: July 15, 2023

Language: Английский

Citations

13
Discovery of Novel Potential Insecticide-Resistance Mutations in Spodoptera frugiperda DOI Creative Commons
Yuhao Cai, Huilin Chen,

Mengfan Hu

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(3), P. 186 - 186

Published: March 11, 2024

The fall armyworm (FAW), Spodoptera frugiperda, is a worldwide agricultural pest that invaded China in 2018, and has developed resistance to multiple insecticides. evolution of insecticide facilitated by mutations target genes responsible for conferring resistance. In this study, amplicon sequencing analyzed 21 sites six genes. addition known mutations, unknown variants were also found, including novel variants: F290C (ace-1 gene, 0.1% frequency), I1040T/V (CHSA A309T (GluCl I4790T/V (RyR frequency). Additionally, molecular docking was employed investigate the impact aforementioned new on binding proteins. analyses indicated abilities reduced, similar reported, implying these may confer transitional This study provide foundation understanding functions evolutionary processes drive emergence invasive species.

Language: Английский

Citations

5