Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Feb. 18, 2025
Biopesticides
have
emerged
as
a
global
trend
to
minimize
the
risks
associated
with
synthetic
agrochemicals.
However,
their
stability
and
efficacies
remain
challenges
for
widespread
application.
Herein,
co-assembled
nanoparticles
(AT
NPs
or
AP
NPs)
based
on
azadirachtin
(AZA)
tannic
acid
(TA)
phenylalanine
(PA)
are
constructed
in
aqueous
solution
through
self-assembly
technology.
The
small
particle
size,
low
PDI,
high
ζ-potential,
related
other
physicochemical
characteristics
of
can
improve
wettability,
adhesiveness,
rain
erosion
resistance,
photostability
compared
commercial
AZA
formulation.
Importantly,
co-assemblies
bidirectional
pH-responsive
disassembly
acidic
alkaline
solutions,
allow
them
respond
microenvironmental
stimuli
targets
enable
controlled
release
AZA.
nanosystems
demonstrated
remarkable
vitro
vivo
insecticidal
activities
against
Ostrinia
furnacalis
Aphis
gossypii.
This
study
illustrates
distinctive
perspective
developing
eco-friendly
nanosystems,
highlighting
water-based
treatment
method
biopesticides
improved
properties
utilization
efficiency.
delivery
limits
Here,
is
plant
secondary
metabolites
efficient
drug
delivery,
demonstrating
pest
management,
activation
defensive
capacity,
while
increasing
production
yield
crops.
Plants,
Journal Year:
2024,
Volume and Issue:
13(2), P. 293 - 293
Published: Jan. 18, 2024
Meloidogyne
enterolobii
is
an
extremely
important
plant
parasitic
nematode.
Tomato
(Solanum
lycopersicum)
essential
worldwide
vegetable,
and
M.
poses
a
major
threat
to
its
production.
The
present
research
investigated
the
effects
of
different
levels
inoculum
density
(100,
500,
1000,
1500,
2000
second-stage
juveniles
(J2s)/plant)
on
tomato
growth,
physiological,
biochemical
changes
at
7,
14,
21,
28
days
post-inoculation
(dpi).
negative
impact
plants
gradually
increased
when
level
increased.
Therefore,
population
densities
(500–2000
J2s/plant)
significantly
(p
<
0.05)
reduced
photosynthetic
pigmentation,
gas
exchange,
chlorophyll
fluorescence
compared
control
plants,
while
low
(100
showed
very
little
influence.
Furthermore,
with
highest
(2000
exhibited
greater
number
egg
masses
galls.
notable
correlation
significant
elevation
both
malondialdehyde
(MDA)
hydrogen
peroxide
(H2O2)
levels,
which
are
recognized
as
detrimental
stresses
in
plants.
Similarly,
rise
activity
several
defensive
antioxidant
enzymes,
namely
superoxide
dismutase
(SOD),
catalase
(CAT),
peroxidase
(POD),
indicates
mechanism
used
combat
oxidative
destruction
produced
by
enterolobii.
specific
glutathione
(GSH)
ascorbate
(ASA)
potent
defense
molecules
response
induced
damage.
In
addition,
our
findings
also
demonstrated
that
secondary
metabolites
responsible
for
scavenging
stress
However,
further
required
explore
underlying
reasons
this
phenomenon
develop
efficient
chemical
or
biocontrol
strategies
managing
Horticulture Research,
Journal Year:
2023,
Volume and Issue:
11(2)
Published: Dec. 29, 2023
Abstract
Powdery
mildew
(PM)
is
one
of
the
most
destructive
diseases
that
threaten
cucumber
production
globally.
Efficient
breeding
novel
PM-resistant
cultivars
will
require
a
robust
understanding
molecular
mechanisms
resistance
against
PM.
Using
genome-wide
association
study,
we
detected
locus
significantly
correlated
with
PM
in
stem,
pm-s5.1.
A
1449-bp
insertion
CsMLO8
coding
region
at
pm-s5.1
resulted
enhanced
stem
resistance.
Knockout
mutants
and
CsMLO11
generated
by
CRISPR/Cas9
both
showed
improved
hypocotyl,
leaves,
double
mutant
mlo8mlo11
displayed
even
stronger
We
found
reactive
oxygen
species
(ROS)
accumulation
was
higher
these
mutants.
Protein
interaction
assays
suggested
could
physically
interact
CsRbohD
CsCRK2,
respectively.
Further,
CsCRK2
competitively
C-terminus
to
affect
CsCRK2-CsRbohD
module-mediated
ROS
during
defense.
These
findings
provide
new
insights
into
CsMLO
proteins
defense
responses.
Annual Research & Review in Biology,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 15
Published: Feb. 8, 2024
In
the
last
few
decades,
research
on
endophytic
fungi
has
been
increasing
due
to
its
applications
in
agriculture,
medicine,
and
pharmaceutical
industry.
Many
studies
indicate
that
fungal
endophytes
produce
various
types
of
bioactive
compounds.
this
study,
fungus
Trichoderma
longibrachiatum
L2D2
was
isolated
from
leaf
medicinal
plant
Anaphalis
contorta
secondary
metabolites
were
collected.
The
crude
extract
analyzed
for
presence
seven
phytochemicals,
it
observed
alkaloids,
flavonoids,
phenolics,
steroids,
tannins,
terpenoids
produced.
It
also
found
endophyte
had
a
total
flavonoid
content
126.07
(µg
quercetin
equivalent/mg)
phenolic
43.97
gallic
acid
equivalent/mg).
Gas
Chromatography–Mass
Spectrometry
(GC-MS)
analysis
reveals
major
compound
Dithiooxamide,
which
retention
time
(RT)
50.030%,
Liquid
(LC-MS)
study
shows
most
abundant
compounds
as
Hexanal
(RT
3.687
min)
positive
ionization
Rosamultin
24.387
negative
ionization.
Fourier-Transform
Infrared
(FTIR)
Spectroscopy
alcohol,
aldehyde,
aliphatic
ether,
primary
amine,
alkene,
alkyl
halide,
aromatic
compound,
carboxylic
acid,
ketone,
phenol,
sulfoxide
functional
groups.
Further
studies,
like
purification
their
biological
activities,
will
reveal
applicability
T.
industries.
The
current
progress
in
genetic
technologies
(methodologies)
allows
to
create
crops
with
the
modified
genome
counter
different
pest
species.
Genome
editing
is
most
popular
and
efficient
approach
increase
crop
defense
against
harmful
insects,
mites,
nematodes.
During
first
decades
of
twenty-first
century,
methodology
control
using
plant
recombinant
has
evolved
significantly.
Toxins
bacteria
Bacillus
thuringiensis
(Bt)
were
heterologous
protective
compounds
introduced
into
plants,
until
now,
these
remain
main
source
transgenes
for
control.
Heterologous
expression
proteins
toxic
pests,
such
as
lectins
protease
inhibitors,
was
another
strategy
increasing
resistance
plants.
RNA
interference
genes
achieved
through
plants
recently
become
studied
area
protection.
CRISPR/Cas-based
modification
systems
modern
gene
manipulation
allow
knock
out
genes,
targeted
variation,
include
precise
insertion/deletion
single
nucleotide
changes.
All
approaches
are
focused
on
targeting
some
physiological,
biochemical,
behavioral
pests.
This
chapter
summarizes
improvement
protection
We
extensively
reviewed
sum
research
articles
published
between
2000
2022
reporting
that
successful
transgenic
events
proved
be
a
wide
range
