Decoding Aging in the Heart via Single Cell Dual Omics of Non-Cardiomyocytes DOI Creative Commons

Y.H. Song,

Wang Li, Haofei Wang

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(12), P. 111469 - 111469

Published: Nov. 28, 2024

Language: Английский

A systems view of the vascular endothelium in health and disease DOI Creative Commons
Hellmut G. Augustin, Gou Young Koh

Cell, Journal Year: 2024, Volume and Issue: 187(18), P. 4833 - 4858

Published: Sept. 1, 2024

Language: Английский

Citations

30
Single-cell transcriptional profiling reveals a novel RAB13+ endothelial subpopulation and profibrotic mesenchymal cells in the aged human bone marrow DOI Open Access
Itziar Cenzano, Miguel Cócera,

A. Perez

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

ABSTRACT The bone marrow (BM) microenvironment plays a crucial role in regulating hematopoiesis, yet the molecular and functional changes associated with aging humans remain poorly understood. Using single-cell RNA sequencing (scRNA-seq), we uncovered transcriptional shifts BM endothelial cells (EC) mesenchymal stem (MSC) during aging. Our analysis revealed that aged sinusoidal EC adopt prothrombotic, exhibit mitochondrial dysfunction, have compromised vascular function. Additionally, identified unique arterial subset, present only individuals, elongation senescence processes characterized by RAB13 expression. MSC from subjects displayed an impaired matrix remodeling epithelial-mesenchymal transition, driven partly subpopulation of THY1 + profibrotic stromal absent young subjects. Aged were also increased ATP-oxidative metabolism reduced protein folding capacity. Finally, using immunofluorescent imaging spatial transcriptomics, confirmed presence senescent samples significant age-related cell-cell communication within niche. In summary, this work provides comprehensive view diversity, cellular interactions, organization MSC, offering novel insights potential targets could be exploited for preventing aged-associated humans.

Language: Английский

Citations

0
Roadmap for alleviating the manifestations of ageing in the cardiovascular system DOI
Luca Liberale, Simon Tual‐Chalot, Simon Sedej

et al.

Nature Reviews Cardiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Language: Английский

Citations

0
Cxcl9 modulates aging associated microvascular metabolic and angiogenic dysfunctions in subcutaneous adipose tissue DOI Creative Commons
Xin Fu, Yu Zhao,

Xiwei Cui

et al.

Angiogenesis, Journal Year: 2025, Volume and Issue: 28(2)

Published: Feb. 11, 2025

Microvascular aging, predominantly driven by endothelial cells (ECs) dysfunction, is a critical early event in cardiovascular diseases. However, the specific effects of aging on ECs across microvascular network segments and associated mechanisms are not fully understood. In this study, we detected rarefaction decreased proportion venular subcutaneous adipose tissue aged mice using light-sheet immunofluorescence microscopy single-cell RNA sequencing. Moreover, ECs, especially subtype, exhibited pseudotemporal transition to terminal state characterized diminished oxidative phosphorylation strengthened cytokine signaling. Metabolic flux balance analysis predicted that among 13 differentially expressed cytokines identified EC subpopulations, Cxcl9 was strongly correlated with impaired ECs. It further validated treated Cxcl9. Notably, G protein-coupled receptor signaling pathway subsequently suppressed, which Aplnr suppression also observed contributing their energy metabolism reduced angiogenesis. Based these findings, propose as biomarker for aging-related dysfunction suggesting targeting may help combat aging.

Language: Английский

Citations

0
Cardiac Aging in the Multi-Omics Era: High-Throughput Sequencing Insights DOI Creative Commons

Y.H. Song,

Brian Spurlock, Jiandong Liu

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(20), P. 1683 - 1683

Published: Oct. 11, 2024

Cardiovascular diseases are a leading cause of mortality worldwide, and the risks both developing disease receiving poor prognosis increase with age. With increasing life expectancy, understanding mechanisms underlying heart aging has become critical. Traditional techniques have supported research into finding physiological changes hallmarks cardiovascular aging, including oxidative stress, disabled macroautophagy, loss proteostasis, epigenetic alterations, among others. The advent high-throughput multi-omics offers new perspectives on molecular cellular processes in heart, guiding development therapeutic targets. This review explores contributions characteristics these to unraveling aging. We discuss how different omics approaches, alone combination, produce robust exciting findings outline future directions prospects studying this era.

Language: Английский

Citations

1
Decoding Aging in the Heart via Single Cell Dual Omics of Non-Cardiomyocytes DOI Creative Commons

Y.H. Song,

Wang Li, Haofei Wang

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(12), P. 111469 - 111469

Published: Nov. 28, 2024

Language: Английский

Citations

0