Phytomedicine, Journal Year: 2024, Volume and Issue: 136, P. 156328 - 156328
Published: Dec. 15, 2024
Language: Английский
Aging advances., Journal Year: 2025, Volume and Issue: 2(1), P. 29 - 39
Published: Feb. 6, 2025
There is a significant global death and disability rate associated with ischemic stroke, making it one of the most prevalent disorders. With increasing worldwide aging population, prevalence stroke has notably increased emerged as pivotal issue in public health. The neurovascular unit primarily consists neurons, astrocytes, microglia, brain microvascular endothelial cells. These cells are structurally tightly connected; together, they maintain balance function crucial for cerebral damage. Emerging studies recent years have demonstrated development associations between vascular neural units. characterizes whole-tissue response to encompassing interrelationship reciprocal impact glial cells, microvessels. results compromised integrity microvasculature blood–brain barrier, neuronal cell death, response, inflammatory immune infiltration, all which strongly course stroke. This narrative review explores complex relationship context an population. aging, undergoes structural functional changes that predispose elderly higher risks complicate recovery post-stroke. We explore on unit, focusing how age-related alterations contribute susceptibility influence repair mechanisms highlights blood-brain barriers, heightened reactions, infiltration critical progression By examining latest research, we aim provide comprehensive understanding unit’s role within population discuss regenerative medicine offers novel strategies treatment, emphasizing neuroprotection, neuroregeneration, neurorepair. aspects paramount importance clinical interventions tailored elderly, who often present unique challenges management. underscores considering factor research cellular interactions form system significantly influenced by process. connections cannot be overlooked quest effective therapies. presents new perspectives particularly where traditional therapies may less or carry risks. aims bridge gap knowledge regarding providing foundation targeted can improve outcomes this vulnerable
Language: Английский
Citations
0
Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119874 - 119874
Published: April 1, 2025
Language: Английский
Citations
0
Phytomedicine, Journal Year: 2024, Volume and Issue: 130, P. 155701 - 155701
Published: May 1, 2024
Language: Английский
Citations
2
PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17534 - e17534
Published: June 27, 2024
Ischemic stroke (IS) is a disease with high mortality and disability rate worldwide, its incidence increasing per year. Angiogenesis after IS improves blood supply to ischemic areas, accelerating neurological recovery. β-asarone has been reported exhibit significant protective effect against hypoxia injury. The ability of improve injury by inducing angiogenesis not distinctly clarified. experimental rats were induced middle cerebral artery occlusion (MCAO), oxygen-glucose deprivation (OGD) model cells constructed using human microvascular endothelial cell line (HMEC-1) cells. Cerebral infarction pathological damage first determined via triphenyl tetrazolium chloride (TTC) hematoxylin eosin (H&E) staining. Then, viability, apoptosis, assessed utilizing counting kit-8 (CCK-8), flow cytometry, spheroid-based angiogenesis, tube formation assays in OGD HMEC-1 Besides, other related proteins identified western blot. study confirms that β-asarone, like nimodipine, can ameliorate damage. also upregulate vascular growth factor A (VEGFA) nitric oxide synthase (eNOS) induce phosphorylation p38. the proves protect expression VEGFA. In vitro experiments affirmed viability suppress apoptosis OGD-mediated promote upregulating This establishes potential for be latent drug therapy.
Language: Английский
Citations
2
Neuroreport, Journal Year: 2024, Volume and Issue: unknown
Published: April 29, 2024
Pyroptosis, a form of programmed cell death, drives inflammation in the context cerebral ischemia/reperfusion. The molecular mechanism pyroptosis underlying ischemia/reperfusion, however, is not fully understood. transient middle artery occlusion was applied to wild-type and caspase-1 knockout mice. 2,3,5-Triphenyltetrazolium chloride-staining immunohistochemistry were used identify ischemic region, western blot immunofluorescence for examination neuronal pyroptosis. expression inflammatory factors behavioral function assessments further conducted examine effects on protection against ischemia/reperfusion injury. Ischemia/reperfusion injury increased pyroptosis-related signals represented by overexpression proteins including gasdermin D (GSDMD). Meanwhile, number GSDMD positive neurons penumbra staining. Compared with mice, those exhibited decreased levels following Furthermore, attack-induced brain infarction, edema, factors, neurological outcomes partially improved data indicate that participates induced-damage, might be involved, it provides some new insights into ischemia.
Language: Английский
Citations
1
Tissue and Cell, Journal Year: 2024, Volume and Issue: 89, P. 102472 - 102472
Published: July 10, 2024
Cerebral ischemia-reperfusion injury involves a series of pathophysiological processes that occur when blood supply is restored after cerebral vascular obstruction, leading to neuronal damage. The AMPK/ERK1/2 signaling pathway has been identified as crucial in this process, although the exact mechanisms underlying induction remain unclear. In study, we investigated involvement oxidative stress damage following by establishing animal and cell models. Our experimental results demonstrated leads damage, including apoptosis mitochondrial dysfunction. Moreover, further experiments showed inhibition AMPK ERK1/2 activity, using U0126 Compound C respectively, could alleviate stress-induced cellular injury, improve morphology function, reduce reactive oxygen species levels, increase superoxide dismutase suppress apoptosis. These findings clearly indicate critical role regulating injury. discoveries study provide theoretical basis for research development neuroprotective therapeutic strategies targeting pathway.
Language: Английский
Citations
0
Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: 227, P. 116446 - 116446
Published: July 20, 2024
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0
Open Medicine, Journal Year: 2024, Volume and Issue: 19(1)
Published: Jan. 1, 2024
Abstract Objective This study aimed to clarify the roles and underlying mechanisms of luteolin in progression cerebral ischemia/reperfusion injury (CIRI). Methods A mouse model CIRI was established using middle artery occlusion (MCAO) method, after which administered. Subsequently, neuronal apoptosis pyroptosis were measured brain tissues each group subjected RNA sequencing. Results Luteolin alleviated MCAO-induced infarction, apoptosis, pyroptosis. sequencing identified 3,379, 2,777, 3,933 differentially expressed genes (DEGs) MCAO vs sham, + luteolin, sham groups, respectively. The DEGs showed enrichment multiple processes, including pattern specification, forebrain development, anion transport, leukocyte migration, regulation cell–cell adhesion, positive response external stimuli, as well calcium, PI3K-AKT, JAK-STAT, NF-kappa B, IL-17, cAMP, cGMP-PKG, Wnt signaling pathways. In addition, Ccl2 Angpt2 interacted more with other top 30 high interaction weights. Finally, RT-qPCR results that induction significantly up-regulated expression Stoml3 , Eomes Ms4a15 down-regulated Nms Ttr Avpr1a ; however, could partially reverse caused by MCAO. Conclusion can alleviate CIRI, may improve targeting their enriched
Language: Английский
Citations
0
Phytomedicine, Journal Year: 2024, Volume and Issue: 136, P. 156328 - 156328
Published: Dec. 15, 2024
Language: Английский
Citations
0