Decreased ATF5 level contributes to improved mitochondrial function in oocytes exposed to vitrification stress DOI Creative Commons

Guizhen Zhou,

Aiju Liu,

Jiachen Bai

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Sept. 20, 2024

Background Mitochondrial unfolded protein response (mtUPR) plays an essential role in the of mitochondria to stress-induced damage. Activating transcription factor 5 (ATF5) can help sustain mitochondrial function and regulate organelle recovery under stress. Vitrification is a stressor that disrupts activity cell homeostasis. However, little known about ATF5 extreme biophysical chemical stresses during oocyte vitrification. Methods The expression mtUPR biomarkers were measured fresh vitrified oocytes. Subsequently, oocytes with deficiency constructed by siRNA microinjection, development analyzed Furthermore, transcriptome analysis was performed uncover molecular network regulated Results In present study, membrane potential ATP levels decreased knockdown oocytes, line phenotypes observed addition, resulted temperature, reduced levels, abnormal dynamics (fusion fission), increased autophagy. Subsequent experiments indicated suppressed knockdown. Interestingly, aberrantly upregulated exposed vitrification Reduced homeostatic level led accumulated potential. Moreover, germinal vesicle breakdown (GVBD) rate detected after vitro maturation. Transcriptome revealed involved stress response, maturation through cAMP-PKA PI3K/AKT pathways. Discussion Our findings indicate initiated stimuli, downregulated state contributes improved results highlight crucial regulation mediating mtUPR.

Language: Английский

Nuclear receptor subfamily 4 group A member 1 promotes myocardial ischemia/reperfusion injury through inducing mitochondrial fission factor-mediated mitochondrial fragmentation and inhibiting FUN14 domain containing 1-depedent mitophagy DOI Creative Commons
Junyan Wang,

Haowen Zhuang,

Lianqun Jia

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(11), P. 4458 - 4475

Published: Jan. 1, 2024

This study investigated the mechanism by which NR4A1 regulates mitochondrial fission factor (Mff)-related and FUN14 domain 1 (FUNDC1)-mediated mitophagy following cardiac ischemia-reperfusion injury(I/R). Our findings showed that damage regulation was positively correlated with pathological pan-apoptosis of myocardial cell mitochondria. Compared wild-type mice (WT), NR4A1-knockout exhibited resistance to injury fission, characterized activation. Results increased expression level, activating mediated Mff restoring phenotype FUNDC1. The inactivation FUNDC1 phosphorylation could not mediate normalization in a timely manner, leading an excessive stress response unfolded proteins imbalance homeostasis. process disrupted quality control network, accumulation damaged mitochondria activation pan-apoptotic programs. data indicate is novel critical target I/R exertsand negative regulatory effects Mff-mediated mito-fission inhibiting FUNDC1-mediated mitophagy. Targeting crosstalk balance between NR4A1-Mff-FUNDC1 potential approach for treating I/R.

Language: Английский

Citations

17
A novel PDE4 inhibitor ZX21011 alleviates neuronal apoptosis by decreasing GSK3β-mediated Drp1 Ser616 phosphorylation in cerebral ischemia reperfusion DOI

Huizhen Wen,

Xingxing Tu,

Fulan Luo

et al.

Chemico-Biological Interactions, Journal Year: 2025, Volume and Issue: unknown, P. 111405 - 111405

Published: Jan. 1, 2025

Language: Английский

Citations

0
Adaptive Divergence and Functional Convergence: The Evolution of Pulmonary Gene Expression in Amphibians of the Qingzang Plateau DOI Open Access
Liming Chang, Wei Zhu,

Qiheng Chen

et al.

Molecular Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

ABSTRACT The Qingzang Plateau, with its harsh environmental conditions—low oxygen, high ultraviolet radiation and significant temperature fluctuations—demands specialised adaptations for survival. While genetic have been extensively studied, gene expression's role in amphibian adaptation to elevations remains understudied. This study analysed pulmonary expression 119 amphibians across the plateau explore how factors shape evolution. Transcriptomic analyses revealed interspecies variation, driven by like temperature, oxygen levels, UVB precipitation. Principal Component Mantel found no correlation between divergence distance. Instead, species‐specific traits pressures were pivotal shaping patterns. PERMANOVA analysis showed had varying impacts on species. For instance, Bufo gargarizans exhibited a strong response multiple factors, while Scutiger boulengeri was less influenced, reflecting diverse adaptive strategies. Functional enrichment highlighted convergence key biological processes, such as energy metabolism, apoptosis autophagy, despite differences. These processes are critical surviving plateau's extremes. findings suggest that evolution Plateau is shaped both diversity pressures. Although profiles vary, they converge essential functions, offering insights into mechanisms extreme environments.

Language: Английский

Citations

0
Preconditioning with Ginsenoside Rg3 mitigates cardiac injury induced by high-altitude hypobaric hypoxia exposure in mice by suppressing ferroptosis through inhibition of the RhoA/ROCK signaling pathway DOI
Junling Liu,

Caixia Pei,

Nan Jia

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 337, P. 118861 - 118861

Published: Sept. 24, 2024

Language: Английский

Citations

2
Decreased ATF5 level contributes to improved mitochondrial function in oocytes exposed to vitrification stress DOI Creative Commons

Guizhen Zhou,

Aiju Liu,

Jiachen Bai

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Sept. 20, 2024

Background Mitochondrial unfolded protein response (mtUPR) plays an essential role in the of mitochondria to stress-induced damage. Activating transcription factor 5 (ATF5) can help sustain mitochondrial function and regulate organelle recovery under stress. Vitrification is a stressor that disrupts activity cell homeostasis. However, little known about ATF5 extreme biophysical chemical stresses during oocyte vitrification. Methods The expression mtUPR biomarkers were measured fresh vitrified oocytes. Subsequently, oocytes with deficiency constructed by siRNA microinjection, development analyzed Furthermore, transcriptome analysis was performed uncover molecular network regulated Results In present study, membrane potential ATP levels decreased knockdown oocytes, line phenotypes observed addition, resulted temperature, reduced levels, abnormal dynamics (fusion fission), increased autophagy. Subsequent experiments indicated suppressed knockdown. Interestingly, aberrantly upregulated exposed vitrification Reduced homeostatic level led accumulated potential. Moreover, germinal vesicle breakdown (GVBD) rate detected after vitro maturation. Transcriptome revealed involved stress response, maturation through cAMP-PKA PI3K/AKT pathways. Discussion Our findings indicate initiated stimuli, downregulated state contributes improved results highlight crucial regulation mediating mtUPR.

Language: Английский

Citations

0