Reactivation of retrotransposable elements is associated with environmental stress and ageing
Nature Reviews Genetics,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 2, 2025
Language: Английский
Prevalent integration of genomic repetitive and regulatory elements and donor sequences at CRISPR-Cas9-induced breaks
Communications Biology,
Journal Year:
2025,
Volume and Issue:
8(1)
Published: Jan. 20, 2025
CRISPR-Cas9
genome
editing
has
been
extensively
applied
in
both
academia
and
clinical
settings,
but
its
genotoxic
risks,
including
large
insertions
(LgIns),
remain
poorly
studied
due
to
methodological
limitations.
This
study
presents
the
first
detailed
report
of
unintended
LgIns
consistently
induced
by
different
Cas9
regimes
using
various
types
donors
across
multiple
gene
loci.
Among
these
insertions,
retrotransposable
elements
(REs)
host
genomic
coding
regulatory
sequences
are
prevalent.
RE
frequencies
3D
organization
analysis
suggest
originate
from
randomly
acquired
fragments
DNA
repair
mechanisms.
Additionally,
significant
full-length
concatemeric
double-stranded
(dsDNA)
donor
integrations
occur
when
is
present.
We
further
demonstrate
that
phosphorylated
dsDNA
reduce
deletions
almost
two-fold
without
compromising
homology-directed
(HDR)
efficiency.
Taken
together,
our
addresses
a
ubiquitous
overlooked
risk
editing,
contributing
valuable
insights
for
safe
use
tools.
A
comprehensive
Cas9-induced,
loci
reveals
widespread
integration
repetitive
elements,
as
well
sequences,
at
targeted
breaks.
Language: Английский
Correlation of Myeloid-Derived Suppressor Cell Expansion with Upregulated Transposable Elements in Severe COVID-19 Unveiled in Single-Cell RNA Sequencing Reanalysis
Biomedicines,
Journal Year:
2024,
Volume and Issue:
12(2), P. 315 - 315
Published: Jan. 29, 2024
Some
studies
have
investigated
the
potential
role
of
transposable
elements
(TEs)
in
COVID-19
pathogenesis
and
complications.
However,
to
best
our
knowledge,
there
is
no
study
examine
possible
association
TE
expression
cell
functions
its
immune
response
at
single-cell
level.
In
this
study,
we
reanalyzed
RNA
seq
data
bronchoalveolar
lavage
(BAL)
samples
obtained
from
six
severe
patients
three
healthy
donors
assess
probable
correlation
with
responses
induced
by
acute
respiratory
syndrome
coronavirus-2
(SARS-CoV-2)
patients.
Our
findings
indicate
that
expansion
myeloid-derived
suppressor
cells
(MDSCs)
may
be
a
characteristic
feature
COVID-19.
Additionally,
significant
increase
MDSCs
was
observed.
This
upregulation
TEs
linked
adaptability
these
their
microenvironments.
Furthermore,
it
appears
identification
overexpressed
pattern
recognition
receptors
(PRRs)
enhance
suppressive
capacity
cells.
Thus,
emphasizes
crucial
functionality
during
Language: Английский
Transposable element expression with variation in sex chromosome number: insights into a toxic Y effect on human longevity
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Aug. 3, 2023
A
bstract
Why
women
live
longer
than
men
is
still
an
open
question
in
human
biology.
Sex
chromosomes
have
been
proposed
to
play
a
role
the
observed
sex
gap
longevity,
and
Y
male
chromosome
has
suspected
of
having
potential
toxic
genomic
impact
on
longevity.
It
hypothesized
that
aging
individuals,
TE
repression
diminished,
which
could
lead
detrimental
effects
(e.g.
somatic
mutations,
perturbed
gene
expression)
acceleration
process.
As
typically
enriched
transposable
elements
(TE),
this
explain
why
presence
associated
with
shorter
Using
transcriptomic
data
from
humans
atypical
karyotypes,
we
found
association
between
expression
number
chromosomes.
These
findings
are
consistent
existence
effect
men's
Language: Английский
Correlation of myeloid-derived suppressor cell expansion with upregulated transposable elements in severe COVID-19 unveiled in single-cell RNA sequencing reanalysis
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Sept. 5, 2023
Abstract
Some
studies
investigated
the
potential
role
of
transposable
elements
(TEs)
in
COVID-19
pathogenesis
and
complications.
However,
to
best
our
knowledge,
there
is
no
study
examine
possible
association
TEs
expression
cell
functions
its
immune
response
at
single-cell
level.
In
this
study,
we
reanalyzed
RNA
seq
data
bronchoalveolar
lavage
(BAL)
samples
obtained
from
six
severe
patients
three
healthy
donors
assess
probable
correlation
TE
with
responses
induced
by
SARS-CoV-2
virus
patients.
Our
findings
indicated
that
expansion
myeloid-derived
suppressor
cells
(MDSCs)
may
be
a
characteristic
feature
COVID-19.
Additionally,
significant
increase
MDSCs
was
observed.
This
upregulation
linked
adaptability
these
their
microenvironments.
Furthermore,
it
appears
identification
overexpressed
Pattern
recognition
receptors
(PRRs)
enhance
suppressive
capacity
cells.
Thus,
emphasizes
crucial
functionality
during
Language: Английский