Association of gut microbiota and immunometabolic profiles with ischemic stroke severity DOI Creative Commons

Claude Pilon,

Dioze Guadagnini, Heloísa Balan Assalin

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 23, 2025

This study investigates the influence of three regulators human homeostasis-intestinal microbiota, immune profile, and circulating metabolites-on stroke severity. We conducted a involving 33 patients with mild/moderate (MS) 32 severe (SS), all assessed during acute phase (first 24 h). The analysis focused on microbiota composition (45 patients), serum metabolomics inflammatory markers (20 patients). SS exhibited more pronounced insulin resistance associated increased levels branched-chain amino acids their metabolites. showed an increase in cytokines IL-6 TNF-α, surprisingly IL-10 butyrate which are anti-inflammatory. also displayed distinct statistically significant differences β-diversity compared to MS group, notably higher prevalence Pseudomonadota (formerly Proteobacteria). In summary, our data indicate that SS, those MS, characterized by insulin-resistant state, key regulators: metabolites, interleukins. These findings provide new insights into regulatory components gut-brain axis, could be developed cost-effective widely accessible therapies for SS.

Language: Английский

Berberine alleviates ETEC-induced intestinal inflammation and oxidative stress damage by optimizing intestinal microbial composition in a weaned piglet model DOI Creative Commons
Yue Wang,

Z. Zhang,

Min Du

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 16, 2024

Introduction Enterotoxigenic Escherichia coli (ETEC) is the main diarrhea-causing pathogen in children and young animals has become a global health concern. Berberine type of “medicine food homology” long history use China, particularly treating gastrointestinal disorders bacterial diarrhea. Methods In this study, we explored effects berberine on growth performance, intestinal inflammation, oxidative damage, microbiota weaned piglet model ETEC infection. Twenty-four piglets were randomly divided into four groups—a control group (fed basal diet [BD] infused with saline), BD+ETEC ETEC), LB+ETEC 0.05% infection), HB+ETEC 0.1% ETEC). Results significantly improved final body weight (BW), average daily gain (ADG), feed intake (ADFI) ( P <0.05) piglets, effectively decreased incidence diarrhea among <0.05). Additionally, downregulated expression levels genes encoding TNF-α, IL-1β, IL-6, IL-8, TLR4, MyD88, NF-κB, IKKα, IKKβ small intestine infection upregulated coding for Nrf2, CAT, SOD1, GPX1, GST, NQO1, HO-1, GCLC, GCLM 12 functional COG categories 7 KEGG signaling pathways. A correlation analysis showed that increased relative abundance beneficial bacteria (Gemmiger, Pediococcus, Levilactobacillus, Clostridium, Lactiplantibacillus, Weissella, Enterococcus, Blautia, Butyricicoccus) pathogenic (Prevotella, Streptococcus, Parabacteroides, Flavonifractor, Alloprevotella) known to be closely related inflammation stress piglets. conclusion, disrupted upregulating TLR4/MyD88/NF-κB Nrf2 pathways, consequently leading stress-induced damage. Discussion Our data indicated can optimize balance modulate thus helping alleviate damage caused by

Language: Английский

Citations

5
Deciphering the tripartite interaction of urbanized environment, gut microbiome and cardio-metabolic disease DOI

Avaneesh Shukla,

Chanchal Sharma, Md. Zubbair Malik

et al.

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 377, P. 124693 - 124693

Published: Feb. 28, 2025

Language: Английский

Citations

0
Association of gut microbiota and immunometabolic profiles with ischemic stroke severity DOI Creative Commons

Claude Pilon,

Dioze Guadagnini, Heloísa Balan Assalin

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 23, 2025

This study investigates the influence of three regulators human homeostasis-intestinal microbiota, immune profile, and circulating metabolites-on stroke severity. We conducted a involving 33 patients with mild/moderate (MS) 32 severe (SS), all assessed during acute phase (first 24 h). The analysis focused on microbiota composition (45 patients), serum metabolomics inflammatory markers (20 patients). SS exhibited more pronounced insulin resistance associated increased levels branched-chain amino acids their metabolites. showed an increase in cytokines IL-6 TNF-α, surprisingly IL-10 butyrate which are anti-inflammatory. also displayed distinct statistically significant differences β-diversity compared to MS group, notably higher prevalence Pseudomonadota (formerly Proteobacteria). In summary, our data indicate that SS, those MS, characterized by insulin-resistant state, key regulators: metabolites, interleukins. These findings provide new insights into regulatory components gut-brain axis, could be developed cost-effective widely accessible therapies for SS.

Language: Английский

Citations

0