Berberine alleviates enterotoxigenic Escherichia coli-induced intestinal mucosal barrier function damage in a piglet model by modulation of the intestinal microbiome

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 14, 2025

Introduction Enterotoxic Escherichia coli (ETEC) is the main pathogen that causes diarrhea, especially in young children. This disease can lead to substantial morbidity and mortality a major global health concern. Managing ETEC infections challenging owing increasing prevalence of antibiotic resistance. Berberine, categorized as substance with similarities “medicine food,” has been used China for hundreds years treat gastrointestinal disorders bacteria-induced diarrhea. study investigated preventive effect dietary berberine on intestinal mucosal barrier induced by microbial community within intestines weaned piglets. Methods Twenty-four piglets were randomly divided into four groups. Piglets administered either standard diet or supplemented at concentrations 0.05 0.1%. orally saline. Results Dietary supplementation reduced diamine oxidase, d-lactate, endotoxin levels infected ( P < 0.05). Berberine increased jejunal villus height, villus/crypt ratio, thickness 0.05), goblet cell numbers villi crypts Furthermore, optical density mucin 2 2, P-glycoprotein, CYP3A4 mRNA expression expressions zonula occludins-1 (ZO-1), occludins-2 (ZO-2), Claudin-1, Occludin, E-cadherin ileum Moreover, BCL2, epithelial apoptosis 0.05) decreased BAX BAK duodenum jejunum, well CASP3 CASP9 IL-1β, IL-6, IL-8, TNF-α, IFN-γ elevated total volatile fatty acids, acetic acid, propionic valeric isovaleric acid Notably, enhanced abundance beneficial bacteria including Enterococcus, Holdemanella, Weissella, Pediococcus, Muribaculum, Colidextribacter, Agathobacter, Roseburia, Clostridium, Fusicatenibacter , Bifidobacterium . Simultaneously, relative harmful pathogenic bacteria, such Prevotella, Paraprevotella, Corynebacterium, Catenisphaera, Streptococcus, Enterobacter Collinsella Discussion alleviated ETEC-induced damage models. associated enhancement physical, chemical, immune functions enhancing microbiota homeostasis.

Language: Английский

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Multi-effects of natural plant bioactive components on intestinal health in pigs: promising feed-antibiotic alternatives?

Journal of Nutrition, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Effect of Gardeniae Fructus Powder on Growth Performance, Antioxidant Capacity, Intestinal Barrier Function, and Colonic Microbiota of Weaned Piglets

Animals, Journal Year: 2025, Volume and Issue: 15(2), P. 221 - 221

Published: Jan. 15, 2025

The present study aimed to explore the effect of GF powder on growth performance, diarrhea rate, antioxidant and immune capacity, intestinal health weaned piglets. A total 144 piglets (8.29 ± 0.11 kg) at 21 d old were randomly assigned four groups, with each treatment consisting six replicate pens, per pen, pen containing three barrows gilts. fed a basal diet supplement 0%, 0.4%, 0.6%, 0.8% (n = 36). Our results indicated that compared diet, F/G rate remarkably decreased in group (p < 0.05). Serum biochemical parameters showed supplementation significantly increased content HDL-C (0.6 levels), IL-6 (0.8% level), IL-10 (0.4, 0.6, Ig G (0.4% level) index capacity serum MDA levels) jejunal ileal (0.4%, Additionally, T-AOC T-SOD CAT GSH-Px gene expression indicate Nrf 2 NQO SOD 1 GCLC GCLM abundance mucosa; HO-1 mucosa Ulteriorly, level villus height jejunum ileum as well villus/crypt ratio Compared 0.4% Occludin 0.05), 0.6% ZO-1, Claudin-1, ZO-1 along Furthermore, colonic microbiota composition Shannon, observed species, Chao indices At phylum level, comparison relative Firmicutes Bacteroidetes genus Prevotella abundance, Coprococcus species copri Blautia obeum In summary, dietary Gardeniae Fructus improved barrier function, which may be associated improvement copri. These findings used feed additive swine weaning.

Language: Английский

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Changes of metabolic parameters, antioxidant capacity, and gut microbiota in response to substitution of ferrous sulfate with iron hydroxy methionine analog chelate in weaned piglets

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 18, 2025

Previous studies have suggested that dietary organic iron offers health advantages compared to its inorganic counterpart. However, the effects of hydroxy methionine analog chelate (Fe-HMA) supplementation in weaned piglets not been fully explored. Therefore, this study aimed investigate replacing ferrous sulfate with Fe-HMA as source on serum biochemistry, antioxidant capacity, and gut microbiota piglets. One hundred twenty were randomly allocated two treatment groups. Each group contained four replicates, 15 pigs per replicate. Piglets fed either 100 mg Fe/kg form (Fe-sulfate group) or 50 (Fe-HMA for 28 days. Results showed supplementing an significantly increased levels triglycerides glucose portal venous serum, albumin both decreased low-density lipoprotein level Additionally, reduced liver malondialdehyde levels, while increasing catalase (CAT), glutathione peroxidase (GSH-Px), total superoxide dismutase, manganese dismutase well GSH-Px CAT liver. Moreover, regulated intestinal composition, notably relative abundance Proteobacteria decreasing microbes involved aromatic_compound_degradation. In conclusion, improved metabolic parameters composition

Language: Английский

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Gut microbiota-associated metabolites in metabolic diseases and their impact from food processing

Food Innovation and Advances, Journal Year: 2024, Volume and Issue: 0(0), P. 1 - 12

Published: Jan. 1, 2024

Language: Английский

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