Separation of Lung Cancer Cells From Mixed Cell Samples Using Aptamer‐Modified Magnetic Beads and Permalloy Micromagnets DOI
Shu‐Hui Lin,

Yun‐Jung Tsai,

Tzu‐Cheng Su

et al.

Electrophoresis, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

ABSTRACT This study involved the design and fabrication of a microfluidic chip integrated with permalloy micromagnets. The device was used aptamer‐modified magnetic beads (MBs) various sizes to successfully separate lung cancer cells from mixture other cells. overall separation efficiency evaluated based on ratios in different outlets inlets chip. results showed efficiencies ranging 43.4% 50.2% for MB between 1.36 4.50 µm. Interestingly, slightly decreased as size MBs increased, contrary predictions. Further examination revealed that larger exerted gravitational force cell‐bound at low flow rates, causing targets settle before reaching main microchannel region. attributed fluidic resistance caused by mismatch inlet tube conduit. An increase cell accumulation observed due gravity. Therefore, definition effective revised exclude effect inlet. Effective were found be 71.6%, 76.4%, 79.4% 1.36, 3.00, µm, respectively. concluded interacted more force, resulting better separation. However, smaller likely evade force. investigation provides valuable insights into isolating using this method, potential clinical application diagnosis treatment.

Language: Английский

Aptamer selection via versatile microfluidic platforms and their diverse applications DOI

Yi-Da Chung,

Yi-Cheng Tsai,

C.W. Wang

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review examines contributions of microfluidic technology to SELEX-based aptamer identification, with alternative methods such as in vivo -like SELEX and Non-SELEX for selecting aptamers discussed critical steps over the past decade.

Language: Английский

Citations

2
Comparative Analysis of Flow behavior During the nanofluid Phase Transition at Serpentine Microchannel Bends DOI
Juhui Chen,

Xu Hanchi,

Dan Li

et al.

Chemical Engineering and Processing - Process Intensification, Journal Year: 2025, Volume and Issue: unknown, P. 110298 - 110298

Published: April 1, 2025

Language: Английский

Citations

0
A portable microfluidic chip device integrated with a real-time CTCs detection model for point-of-care testing of early-stage lung cancer DOI

Asydicky Firmanto,

Tsung-Wei Lin,

Yi-Chun Du

et al.

Biomedical Signal Processing and Control, Journal Year: 2025, Volume and Issue: 109, P. 108059 - 108059

Published: May 7, 2025

Language: Английский

Citations

0
Phage based magnetic capture method as an aid for real time RPA detection of Salmonella spp. in milk DOI Creative Commons
J.S. Liu, Shan Shan, Weihua Lai

et al.

Journal of Dairy Science, Journal Year: 2024, Volume and Issue: 107(9), P. 6528 - 6540

Published: May 15, 2024

Salmonella is a major cause of foodborne diseases worldwide. Conventional rapid assays for detecting in real samples often encounter severe matrix interference or detect the limited number species genus, resulting inaccuracy detection. In this study, we developed method that combined phage-based magnetic capture with time recombinase polymerase amplification (RPA) rapid, highly sensitive, and specific detection milk an ultra-low limit. The Felix O-1 phage-conjugated beads (O-1 pMBs) synthesized showed excellent ability spp. ideal specificity non-Salmonella strains. After pMBs-based separation, limit (LOD) RPA assay was 50 cfu/mL samples, which significantly increased by magnitude 3–4 orders. exhibited high sensitivity (compatibility) 100% (14/14) all tested serotype strains (exclusivity) (7/7) entire process including capture, DNA extraction, completed within 1.5 h. Furthermore, spiked 10 cfu/25 mL were detected positive after cultured buffered peptone water only 3 Therefore, proposed could be alternative accurate Salmonella.

Language: Английский

Citations

3
Separation of Lung Cancer Cells From Mixed Cell Samples Using Aptamer‐Modified Magnetic Beads and Permalloy Micromagnets DOI
Shu‐Hui Lin,

Yun‐Jung Tsai,

Tzu‐Cheng Su

et al.

Electrophoresis, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

ABSTRACT This study involved the design and fabrication of a microfluidic chip integrated with permalloy micromagnets. The device was used aptamer‐modified magnetic beads (MBs) various sizes to successfully separate lung cancer cells from mixture other cells. overall separation efficiency evaluated based on ratios in different outlets inlets chip. results showed efficiencies ranging 43.4% 50.2% for MB between 1.36 4.50 µm. Interestingly, slightly decreased as size MBs increased, contrary predictions. Further examination revealed that larger exerted gravitational force cell‐bound at low flow rates, causing targets settle before reaching main microchannel region. attributed fluidic resistance caused by mismatch inlet tube conduit. An increase cell accumulation observed due gravity. Therefore, definition effective revised exclude effect inlet. Effective were found be 71.6%, 76.4%, 79.4% 1.36, 3.00, µm, respectively. concluded interacted more force, resulting better separation. However, smaller likely evade force. investigation provides valuable insights into isolating using this method, potential clinical application diagnosis treatment.

Language: Английский

Citations

0