Integrative Cancer Therapies,
Journal Year:
2024,
Volume and Issue:
23
Published: Jan. 1, 2024
Background:
Luteolin,
a
common
dietary
flavonoid
found
in
plants,
has
been
shown
to
have
anti-cancer
properties.
However,
its
exact
mechanisms
of
action
non-small
cell
lung
cancer
(NSCLC)
are
still
not
fully
understood,
particularly
role
regulating
broader
genomic
networks
and
specific
gene
targets.
In
this
study,
we
aimed
elucidate
the
microRNAs
(miRNAs)
NSCLC
treated
with
luteolin,
using
A549
cells
as
model
system.
Materials
Methods:
miRNA
profiling
was
conducted
on
luteolin-treated
Exiqon
microarrays,
validation
selected
miRNAs
by
qRT-PCR.
Bioinformatic
analysis
identified
regulatory
roles
biological
processes
pathways
following
luteolin
treatment.
Computational
algorithms
were
employed
identify
potential
target
genes.
transfected
miR-106a-5p
mimic
inhibitor
or
their
corresponding
controls.
The
expression
levels
2
genes,
twist
basic
helix-loop-helix
transcription
factor
1
(TWIST1)
matrix
metallopeptidase
(MMP2),
migration
assessed.
Results:
341
miRNAs,
18
exhibiting
significantly
altered
(
P
<
0.05).
Subsequent
qRT-PCR
confirmed
6
miRNAs.
KEGG
GO
analyses
revealed
significant
alterations
crucial
for
tumor
biology.
TWIST1
MMP2,
which
both
contain
conserved
binding
sites,
exhibited
an
inverse
correlation
miR-106a-5p.
Dual-luciferase
reporter
assays
MMP2
direct
targets
Luteolin
treatment
led
reduction
migration,
further
amplified
overexpression
Conclusion:
inhibits
modulating
landscape,
shedding
light
laying
foundation
miRNA-based
therapeutic
approaches
NSCLC.
Stem Cells Translational Medicine,
Journal Year:
2022,
Volume and Issue:
11(4), P. 356 - 371
Published: Jan. 29, 2022
Aging
is
a
multifaceted
and
complicated
process,
manifested
by
decline
of
normal
physiological
functions
across
tissues
organs,
leading
to
overt
frailty,
mortality,
chronic
diseases,
such
as
skeletal,
cardiovascular,
cognitive
disorders,
necessitating
the
development
practical
therapeutic
approaches.
Stem
cell
aging
one
theories
organismal
aging.
For
decades,
mesenchymal
stem/stromal
cells
(MSCs)
have
been
regarded
viable
ideal
source
for
stem
cell-based
therapy
in
anti-aging
treatment
due
their
outstanding
clinical
characteristics,
including
easy
accessibility,
simplicity
isolation,
self-renewal
proliferation
ability,
multilineage
differentiation
potentials,
immunomodulatory
effects.
Nonetheless,
evidenced
numerous
studies,
MSCs
undergo
functional
deterioration
gradually
lose
stemness
with
systematic
age
vivo
or
extended
culture
vitro,
limiting
applications.
Even
though
our
understanding
processes
behind
MSC
senescence
remains
unclear,
significant
progress
has
achieved
elucidating
aspects
age-related
phenotypic
changes
possible
mechanisms
driving
senescence.
In
this
review,
we
aim
summarize
current
knowledge
morphological,
biological,
stem-cell
marker
alterations
MSCs,
cellular
molecular
that
underlie
senescence,
recent
made
regarding
innovative
techniques
rejuvenate
senescent
combat
aging,
particular
focus
on
interplay
between
niche
well
translational
relevance.
Also,
provide
some
promising
novel
directions
future
research
concerning
MedComm,
Journal Year:
2022,
Volume and Issue:
3(2)
Published: May 18, 2022
Abstract
Epithelial–mesenchymal
transition
(EMT)
is
a
program
wherein
epithelial
cells
lose
their
junctions
and
polarity
while
acquiring
mesenchymal
properties
invasive
ability.
Originally
defined
as
an
embryogenesis
event,
EMT
has
been
recognized
crucial
process
in
tumor
progression.
During
EMT,
cell–cell
cell–matrix
attachments
are
disrupted,
the
cytoskeleton
remodeled
to
enhance
mobility
of
cells.
This
phenotype
largely
driven
by
group
key
transcription
factors,
typically
Snail,
Twist,
ZEB,
through
epigenetic
repression
markers,
transcriptional
activation
matrix
metalloproteinases,
reorganization
cytoskeleton.
Mechanistically,
orchestrated
multiple
pathways,
especially
those
involved
such
TGFβ,
Wnt,
Hedgehog,
Hippo,
suggesting
intrinsic
link
between
embryonic
development
cancer
In
addition,
redox
signaling
also
emerged
critical
modulator.
confers
with
increased
metastatic
potential
drug
resistant
capacity,
which
accounts
for
recurrence
most
clinic
cases.
Thus,
targeting
can
be
therapeutic
option
providing
chance
cure
patients.
Here,
we
introduce
brief
history
summarize
recent
advances
understanding
mechanisms,
well
highlighting
opportunities
treatment.
Cancers,
Journal Year:
2023,
Volume and Issue:
15(7), P. 2128 - 2128
Published: April 3, 2023
Despite
significant
therapeutic
advancements
for
cancer,
an
atrocious
global
burden
(for
example,
health
and
economic)
radio-
chemo-resistance
limit
their
effectiveness
result
in
unfavorable
consequences.
Natural
compounds
are
generally
considered
safer
than
synthetic
drugs,
use
cancer
treatment
alone,
or
combination
with
conventional
therapies,
is
increasingly
becoming
accepted.
Interesting
outcomes
from
pre-clinical
trials
using
Baicalein
medicines
have
been
reported,
some
of
them
also
undergone
clinical
later
stages.
As
a
result,
we
investigated
the
prospects
Baicalein,
naturally
occurring
substance
extracted
stems
Scutellaria
baicalensis
Georgi
Oroxylum
indicum
Kurz,
which
targets
wide
range
molecular
changes
that
involved
development.
In
other
words,
this
review
primarily
driven
by
findings
studies
therapy
several
cell
populations
based
on
promising
research.
The
modifications
numerous
signal
transduction
mechanisms
transcriptional
agents
highlighted
as
major
players
Baicalein’s
anti-malignant
properties
at
micro
level.
These
include
AKT
serine/threonine
protein
kinase
B
(AKT)
well
PI3K/Akt/mTOR,
matrix
metalloproteinases-2
&
9
(MMP-2
9),
Wnt/-catenin,
Poly(ADP-ribose)
polymerase
(PARP),
Mitogen-activated
(MAPK),
NF-κB,
Caspase-3/8/9,
Smad4,
Notch
1/Hes,
Signal
transducer
activator
transcription
3
(STAT3),
Nuclear
factor
erythroid
2-related
2
(Nrf2)/Kelch-like
ECH-associated
protein-1
(Keap
1),
Adenosine
monophosphate-activated
(AMPK),
Src/Id1,
ROS
signaling,
miR
183/ezrin,
Sonic
hedgehog
(Shh)
signaling
cascades.
promise
anti-inflammatory
to
anti-apoptotic/anti-angiogenic/anti-metastatic
medicinal
element
treating
various
malignancies
its
capability
inhibit
malignant
stem
cells,
evidence
synergistic
effects,
design
nanomedicine-based
drugs
altogether
supported
data
presented
study.
Journal of Translational Medicine,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Jan. 3, 2024
Abstract
Breast
cancer
(BC)
is
a
multifaceted
disease
characterized
by
distinct
molecular
subtypes
and
varying
responses
to
treatment.
In
BC,
the
phosphatidylinositol
3-kinase
(PI3K)
pathway
has
emerged
as
crucial
contributor
development,
advancement,
resistance
This
review
article
explores
implications
of
PI3K
in
predictive,
preventive,
personalized
medicine
for
BC.
It
emphasizes
identification
predictive
biomarkers,
such
PIK3CA
mutations,
utility
profiling
guiding
treatment
decisions.
The
also
discusses
potential
targeting
preventive
strategies
customization
therapy
based
on
tumor
stage,
subtypes,
genetic
alterations.
Overcoming
inhibitors
exploring
combination
therapies
are
addressed
important
considerations.
While
this
field
holds
promise
improving
patient
outcomes,
further
research
clinical
trials
needed
validate
these
approaches
translate
them
into
practice.
Graphical
Journal of Biological Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown, P. 108179 - 108179
Published: Jan. 1, 2025
Highlights:•The
fat
body
undergoes
lipophagy
during
insect
metamorphosis.•The
steroid
hormone
20E
upregulates
Akh
and
Akhr
expression.•The
AKH
pathway
increases
hemolymph
glucose
levels
by
promoting
gluconeogenesis.•High
promote
FOXO
acetylation
nuclear
localization
to
induce
gene
expression
of
lipophagy.AbstractLipophagy
is
a
way
degrade
lipids;
however,
the
molecular
mechanisms
are
not
fully
understood.
Using
holometabolous
lepidopteran
Helicoverpa
armigera,
cotton
bollworm,
as
model,
we
revealed
that
larval
metamorphosis,
essential
for
metamorphosis.
The
20-hydroxyecdysone
(20E)
induced
peptide
adipokinetic
(AKH,
analog
glucagon)
receptor
(AKHR).
was
highly
expressed
in
brain
various
tissues.
upregulated
its
EcR
AKHR
increased
via
gluconeogenesis
promoted
lipophagy.
high
level
lipases
autophagy
genes.
Thus,
brain-adipose
tissue
axis
pathway,
which
presented
nutrients
energy
pupal
adult
development
metamorphosis
after
feeding
stops.
