3D spheroid model of adipose-derived stem cell and breast cancer cell co- culture for mimicking cell migration and the cancer environment DOI Creative Commons
Dong-Woo Kim, Changheon Kim,

Chaewon Bae

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 12, 2023

Abstract Traditional two-dimensional (2D) cell culture methodologies exhibit significant limitations, notably their failure to replicate the intricate three-dimensional (3D) milieu of human tissue architecture. This discrepancy has catalyzed exploration advanced drug screening platforms that more faithfully mimic complex 3D biological environment. To this end, precision medicine research increasingly used in vitro models emulate microenvironment body. In study, breast cancer lines (MDA-MB-231 and SK-BR-3) were co-cultured with adipose-derived stem cells (ADSCs), mimicking tumor multicellular heterogeneous nature solid tumors compared a 2D only system. Additionally, collagen type I was within spheroid platform, enhancing accuracy platform characteristics. Significantly, interactions between ADSCs two markedly influenced factors such as elongation, molecular expression, migration patterns, sensitivity. The integration pivotal simulating microenvironment, highlighting even same line, varying microenvironmental contexts can lead vastly different experimental results. Thus, study provides insights into role cellular substrates akin body when constructing system based on cells.

Language: Английский

Tumor dormancy and relapse: understanding the molecular mechanisms of cancer recurrence DOI Creative Commons
Muhammad Tufail, Canhua Jiang, Ning Li

et al.

Military Medical Research, Journal Year: 2025, Volume and Issue: 12(1)

Published: Feb. 11, 2025

Abstract Cancer recurrence, driven by the phenomenon of tumor dormancy, presents a formidable challenge in oncology. Dormant cancer cells have ability to evade detection and treatment, leading relapse. This review emphasizes urgent need comprehend dormancy its implications for recurrence. Despite notable advancements, significant gaps remain our understanding mechanisms underlying lack reliable biomarkers predicting provides comprehensive analysis cellular, angiogenic, immunological aspects dormancy. It highlights current therapeutic strategies targeting dormant cells, particularly combination therapies immunotherapies, which hold promise preventing By elucidating these proposing innovative research methodologies, this aims deepen ultimately facilitating development more effective recurrence improving patient outcomes.

Language: Английский

Citations

2
Multiple aspects of matrix stiffness in cancer progression DOI Creative Commons
Alessandro Mancini, Maria Teresa Gentile, Francesca Pentimalli

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: July 2, 2024

The biophysical and biomechanical properties of the extracellular matrix (ECM) are crucial in processes cell differentiation proliferation. However, it is unclear to what extent tumor cells influenced by changes surrounding microenvironment how this response varies between different forms, over course progression. entire ensemble genes encoding ECM associated proteins called matrisome. In cancer, evolves become highly dysregulated, rigid, fibrotic, serving both pro-tumorigenic anti-tumorigenic roles. Tumor desmoplasia characterized a dramatic increase α-smooth muscle actin expressing fibroblast deposition hard containing collagen, fibronectin, proteoglycans, hyaluronic acid common many solid tumors. review, we described role inflammation inflammatory cytokines, desmoplastic remodeling, state transition driven forces signaling pathways mechanotransduction as potential targeted therapies, focusing on impact qualitative quantitative variations regulation development, hypothesizing presence matrisome drivers, acting alongside cell-intrinsic oncogenic some stages neoplastic progression contexts, such pancreatic carcinoma, breast lung cancer mesothelioma.

Language: Английский

Citations

14
Dormant cancer cells and polyploid giant cancer cells: The roots of cancer recurrence and metastasis DOI Creative Commons

Yuqi Jiao,

Yongjun Yu,

Minying Zheng

et al.

Clinical and Translational Medicine, Journal Year: 2024, Volume and Issue: 14(2)

Published: Feb. 1, 2024

Abstract Tumour cell dormancy is critical for metastasis and resistance to chemoradiotherapy. Polyploid giant cancer cells (PGCCs) with or multiple nuclei high DNA content have the properties of stem single PGCCs can individually generate tumours in immunodeficient mice. represent a dormant form that survive harsh tumour conditions contribute recurrence. Hypoxic mimics, chemotherapeutics, radiation cytotoxic traditional Chinese medicines induce formation through endoreduplication and/or fusion. After incubation, recover from treatment produce daughter strong proliferative, migratory invasive abilities via asymmetric division. Additionally, resist hypoxia chemical stress distinct protein signature involves chromatin remodelling cycle regulation. Dormant cellular basis therapeutic resistance, metastatic cascade disease This review summarises regulatory mechanisms governing entry exit dormancy, which may be used by PGCCs, potential strategies targeting PGCCs.

Language: Английский

Citations

12
Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive review DOI Creative Commons
Lai Wei,

Hu Geliang,

Bin Xu

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: March 3, 2025

Abstract As an emerging technology, organoids are promising new tools for basic and translational research in disease. Currently, the culture of relies mainly on a type unknown composition scaffold, namely Matrigel, which may pose problems studying effect mechanical properties organoids. Hydrogels, material with adjustable properties, can adapt to current studies. In this review, we summarized synthesis recent advance developing definite hydrogel scaffolds organoid identified critical parameters regulating properties. addition, classified by different like stiffness viscoelasticity, concluded development tumor We hope review enhances understanding hydrogels provides more practical approaches investigating them.

Language: Английский

Citations

1
New Insights into YAP/TAZ-TEAD-Mediated Gene Regulation and Biological Processes in Cancer DOI Open Access
Yang Zhao,

Marisela Sheldon,

Yutong Sun

et al.

Cancers, Journal Year: 2023, Volume and Issue: 15(23), P. 5497 - 5497

Published: Nov. 21, 2023

The Hippo pathway is conserved across species. Key mammalian kinases, including MST1/2 and LATS1/2, inhibit cellular growth by inactivating the TEAD coactivators, YAP, TAZ. Extensive research has illuminated roles of signaling in cancer, development, regeneration. Notably, dysregulation components not only contributes to tumor metastasis, but also renders tumors resistant therapies. This review delves into recent on YAP/TAZ-TEAD-mediated gene regulation biological processes cancer. We focus several key areas: newly identified molecular patterns YAP/TAZ activation, emerging mechanisms that contribute metastasis cancer therapy resistance, unexpected suppression, advances therapeutic strategies targeting this pathway. Moreover, we provide an updated view YAP/TAZ's functions, discuss ongoing controversies, offer perspectives specific debated topics rapidly evolving field.

Language: Английский

Citations

20
Unveiling cancer dormancy: Intrinsic mechanisms and extrinsic forces DOI Creative Commons
Ruihua Liu, Yawei Zhao, Shang Su

et al.

Cancer Letters, Journal Year: 2024, Volume and Issue: 591, P. 216899 - 216899

Published: April 21, 2024

Tumor cells disseminate in various distant organs at early stages of cancer progression. These disseminated tumor (DTCs) can stay dormant/quiescent without causing patient symptoms for years or decades. dormant survive despite curative treatments by entering growth arrest, escaping immune surveillance, and/or developing drug resistance. However, these reactivate to proliferate, metastatic progression relapse, posing a threat patients' survival. It's unclear how maintain dormancy and what triggers their reactivation. What are better approaches prevent relapse through harnessing dormancy? To answer remaining questions, we reviewed the studies reactivation types using different model systems, including brief history studies, intrinsic characteristics cells, external cues cellular molecular levels. Furthermore, discussed future directions field strategies manipulating recurrence.

Language: Английский

Citations

8
Cancer quiescence: non-coding RNAs in the spotlight DOI
Konstantinos Soureas, M. Papadimitriou, Konstantina Panoutsopoulou

et al.

Trends in Molecular Medicine, Journal Year: 2023, Volume and Issue: 29(10), P. 843 - 858

Published: July 27, 2023

Language: Английский

Citations

14
Cell cycle control by cell-matrix interactions DOI Creative Commons

Michael John Jones,

Matthew C. Jones

Current Opinion in Cell Biology, Journal Year: 2023, Volume and Issue: 86, P. 102288 - 102288

Published: Dec. 5, 2023

Cell adhesion to the extracellular matrix (ECM) is required for normal cell cycle progression and accurate division. However, how wide range of ECM proteins found in human tissues influences not fully understood. The composition physical properties can have profound effects on proliferation but also promote exit quiescence. Furthermore, during tumor development progression, changes drive both cancer dormancy. Cell-matrix primarily sensed via integrin-associated complexes, which turn are regulated by machinery. In particular, cyclin-dependent kinase 1 (CDK1) has been shown play a crucial role regulating complexes interphase entry into mitosis. These reciprocal links between cell-matrix interactions now being identified.

Language: Английский

Citations

12
Tumor Dormancy and Reactivation: The Role of Heat Shock Proteins DOI Creative Commons
Haneef Ahmed Amissah, Stephanie E. Combs, Maxim Shevtsov

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(13), P. 1087 - 1087

Published: June 23, 2024

Tumors are a heterogeneous group of cell masses originating in various organs or tissues. The cellular composition the tumor mass interacts an intricate manner, influenced by humoral, genetic, molecular, and microenvironment cues that dictate growth suppression. As result, tumors undergo period dormant state before their clinically discernible stage, which surpasses clinical dormancy threshold. Moreover, as genetically imprinted strategy, early-seeder cells, distinct population break off to dock nearby extravasate into blood vessels secondary tissues, where they form disseminated solitary cells with reversible capacity. Among mechanisms underlying formation, heat shock proteins (HSPs) might play one most important roles how program plays out. It is known numerous aberrant processes, such malignant transformation, cancer stemness, invasion, metastasis, angiogenesis, signaling pathway maintenance, HSPs. An accumulating body knowledge suggests HSPs may be involved angiogenic switch, immune editing, extracellular matrix (ECM) remodeling cascades, crucial strategies initiation maintenance program. In this review, we highlight biological events orchestrate work has been conducted on dynamics mass, well reactivation. Additionally, propose conceptual framework could possibly underlie reactivation metastatic relapse.

Language: Английский

Citations

4
Regulation of Brain Metastatic Breast Cancer Cell Dormancy versus Proliferation on Hyaluronic Acid Hydrogels via Laminin and Laminin-Derived Peptides DOI

Kasra Goodarzi,

Pallab Sarker, Shreyas S. Rao

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Among the secondary target organs for metastatic breast cancer, brain metastasis is extremely aggressive in nature, resulting lower survival rates. These cancer cells have potential to enter a dormant state brain, allowing them survive extended time periods. The microenvironment plays key role controlling phenotype, yet how various components of this influence dormancy not well understood. In work, we employed hyaluronic acid (HA)-based hydrogels as mimetic tissue environment study biochemical cues, specifically, impact laminin and laminin-derived peptides IKVAV YIGSR on regulation cell versus proliferation. We applied varying protein/peptide concentrations confirmed functionalization HA hydrogel surfaces. then seeded 10,000 surface cultured 5 days. found that presence or IKVAV, MDA-MB-231Br transitioned from rounded spread morphology exhibited enhanced proliferation laminin/IKVAV concentration increased. contrast, functionalized with YIGSR, these maintained morphology, no concentrations. involvement αVβ3 integrin mediating tumor phenotype laminin. By evaluating known markers proliferation, direct correlation between increased phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK) positivity, along decreased p38 (p-p38) while levels both p-ERK p-p38 remained unaltered. Finally, demonstrated when were transferred IKVAV-deficient IKVAV-rich hydrogels, induced cellular was reversible. Collectively, our findings provide insights into cues regulate

Language: Английский

Citations

0