Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)
Published: Nov. 9, 2024
In recent years, the anti-tumor effects of vitamin D have garnered increasing attention. However, previous epidemiological studies on relationship between and nasopharyngeal carcinoma (NPC) yielded inconsistent results. This study aims to further explore whether helps reduce risk NPC through Mendelian randomization (MR) meta-analysis.
Language: Английский
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Jan. 2, 2025
Nasopharyngeal carcinoma (NPC) presents significant treatment challenges due to its complex etiology and late-stage diagnosis. The traditional Chinese medicine Selaginella doederleinii Hieron (S. doederleinii) has shown potentiality in NPC multi-target, multi-pathway anti-cancer mechanisms. First, we identified related target genes from databases like GeneCards, OMIM, DisGeNET, performed WGCNA analysis on the GSE53819 dataset identify several important gene modules NPC. Active components their targets S. were screened TCMSP other databases, identifying 32 overlapping genes. Gene Ontology (GO) Kyoto Encyclopedia of Genes Genomes (KEGG) pathway revealed that these are primarily involved critical biological processes protein phosphorylation cell cycle regulation. A protein–protein interaction network was constructed, cytoHubba six key (BCL2, MAPK14, ABCB1, PLK1, ATM, HMOX1). Kaplan–Meier immune infiltration further showed closely prognosis microenvironment patients. Single-cell RNA sequencing expression distribution across different types explored roles differentiation process malignant cells through pseudotime trajectory analysis. Molecular docking dynamics simulation results indicated Berberine-MAPK14 Matairesinol-PLK1 complexes have high binding affinity stability. Binding free energy calculations confirmed stability complexes. Based our comprehensive multi-level analysis, active may play a role multi-target synergistic effects.
Language: Английский
Citations
1
Ageing Research Reviews, Journal Year: 2024, Volume and Issue: unknown, P. 102613 - 102613
Published: Dec. 1, 2024
Language: Английский
Citations
5
Annals of Medicine, Journal Year: 2025, Volume and Issue: 57(1)
Published: Jan. 21, 2025
Background Chemoimmunotherapy is the first-line therapy for patients with recurrent or metastatic nasopharyngeal carcinoma (NPC) and currently main induction treatment option locoregionally advanced NPC. However, it remains unclear whether combining immunotherapy standard chemotherapy enhances its efficacy. This study aimed to evaluate efficacy, toxicity, survival outcomes of chemoimmunotherapy in
Language: Английский
Citations
0
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 24, 2025
Abstract Nasopharyngeal carcinoma (NPC) is an Asia‐prevalent malignancy, yet its genetic underpinnings remain incompletely understood. Here, a transcriptome‐wide association study (TWAS) conducted on NPC, leveraging gene expression prediction models based epithelial tissues and genome‐wide (GWAS) summary statistics from 1577 NPC cases 6359 controls of southern Chinese descent. The TWAS identifies VAMP8 chromosome 2p11.2 as novel susceptibility for NPC. Further fine‐mapping analyses pinpoint rs1058588, located within , causal variant through eQTL colocalization, GWAS across multiple cohorts, achieving significance (OR = 1.18, P 3.09 × 10 −10 ). Functional assays demonstrate that exerts tumorigenic role in enhancing cell proliferation, migration, tumor growth. Mechanically, it uncovered rs1058588 modulates by altering binding affinity to miR‐185 . Furthermore, the results show interacts with DHX9 facilitate nuclear recruitment p65, activating NF‐κB pathway. Collectively, findings shed light predisposition underscore critical functional axis involving miR‐185, VAMP8, DHX9, pathway pathogenesis.
Language: Английский
Citations
0
Journal of Dentistry, Journal Year: 2025, Volume and Issue: unknown, P. 105583 - 105583
Published: Jan. 1, 2025
Language: Английский
Citations
0
Phytomedicine, Journal Year: 2025, Volume and Issue: 140, P. 156484 - 156484
Published: Feb. 27, 2025
Squalene epoxidase (SQLE), a key enzyme in cholesterol metabolism, remains underexplored nasopharyngeal carcinoma (NPC). Additionally, the therapeutic potential of Fangchinoline, an alkaloid with anticancer properties, has yet to be systematically evaluated. This research investigates Fangchinoline's efficacy NPC treatment and SQLE-related mechanisms. Drug screening cell lines C666-1 5-8F identified candidates. IC50 values were determined using CCK-8 assays, apoptosis, proliferation, invasion assessed via Annexin V/PI staining, EdU Transwell assays. Cholesterol levels quantified TG kit. RNA sequencing GO/KEGG analyses pathways. Correlation analysis was performed cBioPortal GEPIA2 databases, protein interaction networks STRING Cytoscape, survival Kaplan-Meier curves. Gene expression validated qPCR Western blot, mouse model confirmed vivo efficacy. Fangchinoline inhibited induced reduced accumulation. revealed that downregulated SQLE expression, suppressing PI3K/AKT pathway. highlighted SQLE's role progression, its clinical relevance. By targeting disrupting suppressed tumor growth both vitro vivo. Our study demonstrates inhibits by pathway, providing new insights into as target NPC.
Language: Английский
Citations
0
Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16
Published: Feb. 26, 2025
Nasopharyngeal carcinoma (NPC) is a prevalent malignancy in certain regions, with radiotherapy as the standard treatment. However, resistance to remains critical challenge, necessitating identification of novel biomarkers and therapeutic targets. The tumor-associated microbiota metabolites have emerged potential modulators outcomes. This study included 22 NPC patients stratified into radiotherapy-responsive (R, n = 12) radiotherapy-non-responsive (NR, 10) groups. Tumor tissue fecal samples were subjected 16S rRNA sequencing profile composition targeted metabolomics quantify short-chain fatty acids (SCFAs). XGBoost algorithm was applied identify microbial taxa associated response, quantitative PCR (qPCR) used validate key findings. Statistical analyses conducted assess differences diversity, relative abundance, metabolite levels between Significant alpha diversity at species level observed R NR Bacteroides acidifaciens enriched group, while Propionibacterium acnes Clostridium magna more abundant group. Machine learning identified Acidosoma, acnes, predictors response. Metabolomic profiling revealed elevated acetate implicating its role tumor growth immune evasion. Validation via qPCR confirmed differential abundance these both samples. Our findings highlight interplay profiles influencing outcomes NPC. These results suggest that targeting microbiota-metabolite axis may enhance efficacy
Language: Английский
Citations
0
Cancer Immunology Immunotherapy, Journal Year: 2025, Volume and Issue: 74(4)
Published: March 1, 2025
Immune checkpoint inhibitors (ICIs), particularly PD-1/PD-L1 inhibitors, have demonstrated significant survival benefits in treating recurrent or metastatic nasopharyngeal carcinoma (R/M-NPC). While baseline peripheral blood lymphocyte subsets been identified as prognostic biomarkers various cancers treated with ICIs, their relevance R/M-NPC has not extensively studied. This post hoc analysis used data from 153 patients PD-L1 inhibitor monotherapy the phase 2 trial KL167-2-05-CTP. The subsets, including total T cells, CD4/CD8 ratio, helper suppressor cytotoxic NK and B were tested by flow cytometry. These grouped using optimal cutoff values Maximally Selected Log-rank Statistic. Overall (OS) progression-free (PFS) assessed Kaplan–Meier Cox regression analysis, logistic evaluated associations objective response rate (ORR) disease control (DCR). Patients lower cell percentages showed significantly longer OS (26.3 vs. 12.1 months, p < 0.001) PFS (5.5 3.7 compared to those higher percentages. No differences observed for other subsets. High risk factors shorter (HR, 2.49) 1.62). There no ORR DCR between high low Lower are associated improved undergoing therapy.
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: March 3, 2025
This study aimed to analyze S100A9 and CCL5 levels in patients with nasopharyngeal carcinoma (NPC) evaluate their predictive value as blood-based indicators for NPC diagnosis. Serum were measured 123 newly diagnosed 107 without NPC. Additionally, 38 (19 19 without) recruited from Xiangya Hospital an external validation cohort. Logistic regression was used identify risk factors Variable selection conducted using least absolute shrinkage operator (LASSO) regression. prediction models developed four machine-learning algorithms, performance evaluated ROC curves. Calibration curves, decision curve analysis (DCA), Shapley additive explanation plots employed further evaluation interpretation. significantly elevated compared Multivariate logistic identified S100A9, CCL5, TP, ALB independent predictors of demonstrated that had superior diagnostic other blood indicators, effectively differentiating non-NPC cases. A machine-learning-based model incorporating ALB, GLB, PLR a reliable NPC, achieving Area under the (AUC) 0.877 training The calibration showed excellent agreement between predicted actual probabilities; contrast, DCA highlighted strong clinical utility. also performed well cohort, AUC 0.817. such have values based on these biomarkers robust utility
Language: Английский
Citations
0
Oncotarget, Journal Year: 2025, Volume and Issue: 16(1), P. 133 - 135
Published: Jan. 20, 2025
Language: Английский
Citations
0