BMC Cardiovascular Disorders, Journal Year: 2024, Volume and Issue: 24(1)
Published: Dec. 30, 2024
To screen Myocardial ischemia-reperfusion Injury in mice. adenosine monophate-activatedprotein kinase (AMPK) -related differentially expressed circularRNA (circRNA) MIRI model, Ampk-related circRNA network was drawn to provide possible ideas for the prevention and treatment of MIRI.
Language: Английский
World Journal of Cardiology, Journal Year: 2025, Volume and Issue: 17(1)
Published: Jan. 21, 2025
BACKGROUND Myocardial ischemia/reperfusion (I/R) injury, which is associated with high morbidity and mortality, a main cause of unexpected myocardial injury after acute infarction. However, the underlying mechanism remains unclear. Circular RNAs (circRNAs), are formed from protein-coding genes, can sequester microRNAs or proteins, modulate transcription interfere splicing. Authoritative studies suggest that circRNAs may play an important role in I/R injury. AIM To explore METHODS We constructed model using ligation left anterior descending coronary artery, evaluated success validated triphenyltetrazolium chloride hematoxylin-eosin staining. Then, ventricular samples different groups were selected for mRNA-sequence, differential gene screening was performed on obtained results. The differentially mRNAs divided into up-regulated down-regulated according to their expression levels, Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) functional enrichment analysis performed, respectively. circRNA microRNA (miRNA) paired analysis, binding sites miRNA mRNA virtual screened. Finally, circRNA, by ceRNA mutual most useful network. RESULTS used RNA sequencing array investigate signatures three group sham group. A total 142 upregulated 121 downregulated found be expressed (fold change ≥ 2, P < 0.05). GO KEGG analyses these performed. revealed involved mainly cellular intracellular processes. demonstrated 6 top 20 pathways correlated cell apoptosis. Furthermore, circRNA-miRNA coexpression network based genes constructed, revealing mmu-circ-0001452, mmu-circ-0001637, mmu-circ-0000870 might key regulators CONCLUSION This research provides new insights I/R, expected therapeutic targets
Language: Английский
Citations
0
Antioxidants, Journal Year: 2025, Volume and Issue: 14(3), P. 340 - 340
Published: March 14, 2025
Delayed reperfusion of the ischemic heart (I/R) is known to impair recovery cardiac function and produce a wide variety myocardial defects, including ultrastructural damage, metabolic alterations, subcellular Ca2+-handling abnormalities, activation proteases, changes in gene expression. Although I/R injury has been reported induce formation reactive oxygen species (ROS), inflammation, intracellular Ca2+ overload, generation oxidative stress considered play critical role development dysfunction. Increases production superoxide, hydroxyl radicals, oxidants, such as hydrogen peroxide hypochlorous acid, occur hearts subjected injury. In fact, mitochondria are major source excessive ROS due impairment electron transport system well xanthine oxidase NADPH oxidase. Nitric oxide synthase, mainly present endothelium, also activated injury, leading nitric oxide, which, upon combination with superoxide generates nitrosative stress. Alterations function, sarcolemma, sarcoplasmic reticulum activities, mitochondrial phosphorylation, protease simulated exposing oxyradical-generating (xanthine plus oxidase) or H2O2. On other hand, endogenous antioxidants dismutase, catalase, glutathione peroxidase, concentration transcription factor (Nrf2), which modulates expression various antioxidants, depressed hearts. Furthermore, pretreatment catalase N-acetylcysteine, mercaptopropionylglycerine observed attenuate I/R-induced handling Ca2+-regulatory activities; additionally, it found depress improve function. These observations indicate that intimately involved pathological effects different alterations Thus, we faced task developing safe effective agents for upregulating therapy
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6430 - 6430
Published: June 11, 2024
Coronary artery disease (CAD) and hypertension significantly contribute to cardiovascular morbidity mortality. MicroRNAs (miRNAs) have recently emerged as promising biomarkers therapeutic targets for these conditions. This systematic review conducts a thorough analysis of the literature, with specific focus on investigating miRNA expression patterns in patients CAD hypertension. encompasses an unspecified number eligible studies that employed variety patient demographics research methodologies, resulting diverse profiles. highlights complex involvement miRNAs potential advances diagnostic strategies. Future endeavors are imperative validate findings elucidate precise roles progression, offering avenues innovative tools targeted interventions.
Language: Английский
Citations
3
Published: May 28, 2024
Coronary artery disease (CAD) and hypertension significantly contribute to cardiovascular morbidity mortality. MicroRNAs (miRNAs) have recently emerged as promising biomarkers therapeutic targets for these conditions. This systematic review conducts a thorough analysis of the literature, with specific focus on investigating miRNA expression patterns in patients CAD hypertension. The encompasses an unspecified number eligible studies that employed variety patient demographics research methodologies, resulting diverse profiles. highlights complex involvement miRNAs potential advances diagnostic strategies. Future endeavours are imperative validate findings elucidate precise roles progression, offering avenues innovative tools targeted interventions.
Language: Английский
Citations
1
BMC Cardiovascular Disorders, Journal Year: 2024, Volume and Issue: 24(1)
Published: Dec. 30, 2024
To screen Myocardial ischemia-reperfusion Injury in mice. adenosine monophate-activatedprotein kinase (AMPK) -related differentially expressed circularRNA (circRNA) MIRI model, Ampk-related circRNA network was drawn to provide possible ideas for the prevention and treatment of MIRI.
Language: Английский
Citations
0