Poly
(lactic-co-glycolic
acid)
(PLGA)
is
widely
used
in
bone
repair
due
to
its
good
controlled
degradation.
However,
hydrophobicity,
acidic
products
during
degradation
and
insufficient
osteogenic
capacity
limit
application
repair.
In
this
study,
we
developed
a
PLGA
composite
containing
Mg(OH)2
Zn3(PO4)2
with
the
aim
balance
improve
anti-inflammatory
properties
capacity.
The
physicochemical
properties,
behavior,
antimicrobial
ability,
vitro
biological
of
composites
were
systematically
investigated.
results
showed
that
addition
effectively
neutralized
microenvironment
composites,
moderate
amount
had
little
effect
on
but
an
excessive
accelerated
composites.
provided
excellent
ability.
appropriate
amounts
magnesium
could
promote
proliferation
adhesion
osteoblasts
expression
osteogenesis-related
genes.
have
demonstrated
great
potential
orthopedic
applications
PLGA/Mg(OH)2/Zn3(PO4)2
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(8), P. 7513 - 7513
Published: April 19, 2023
Recently,
substantial
attention
has
been
paid
toward
adipose-derived
mesenchymal
stem
cells
(AdMSCs)
as
a
potential
therapy
in
tissue
engineering
and
regenerative
medicine
applications.
Rat
AdMSCs
(r-AdMSCs)
are
frequently
utilized.
However,
the
influence
of
adipose
depot
site
on
multilineage
differentiation
r-AdMSCs
is
still
ambiguous.
Hence,
main
objective
this
study
was
to
explore
harvesting
location
ability
express
stem-cell-related
markers
pluripotency
genes,
well
their
capacity,
for
first
time.
Herein,
we
have
isolated
from
inguinal,
epididymal,
peri-renal,
back
subcutaneous
fats.
Cells
were
compared
terms
phenotype,
immunophenotype,
expression
genes
using
RT-PCR.
Additionally,
investigated
(adipogenic,
osteogenic,
chondrogenic)
induction
special
stains
confirmed
by
related
RT-qPCR.
All
could
positively
cell
marker
CD
90
105
with
no
significant
in-between
differences.
they
did
not
hematopoietic
34
45.
be
induced
successfully.
epididymal
inguinal
presented
highest
capacity
adipogenic
osteogenic
(21.36-fold
11.63-fold
OPN,
29.69-fold
26.68-fold
BMP2,
37.67-fold
22.35-fold
BSP,
respectively,
(
Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: June 27, 2024
Exosomes,
as
a
class
of
small
extracellular
vesicles
closely
related
to
the
biological
behavior
various
types
tumors,
are
currently
attracting
research
attention
in
cancer
diagnosis
and
treatment.
Regarding
diagnosis,
stability
their
membrane
structure
wide
distribution
body
fluids
render
exosomes
promising
biomarkers.
It
is
expected
that
exosome-based
liquid
biopsy
will
become
an
important
tool
for
tumor
future.
For
treatment,
exosomes,
“golden
communicators”
between
cells,
can
be
designed
deliver
different
drugs,
aiming
achieve
low-toxicity
low-immunogenicity
targeted
delivery.
Signaling
pathways
exosome
contents
also
used
safer
more
effective
immunotherapy
against
tumors.
Exosomes
derived
from
range
sources,
exhibit
characteristics
well
clinical
application
advantages
therapies.
In
this
review,
we
analyzed
main
sources
have
great
potential
broad
prospects
therapy.
Moreover,
compared
therapeutic
advantages,
providing
new
ideas
exosomes.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(2), P. 510 - 510
Published: Jan. 9, 2025
Mesenchymal
stem
cells
(MSCs)
are
multipotent
with
the
potential
to
differentiate
into
various
lineages.
They
have
also
protect
themselves
against
harmful
stimuli
maintain
their
functional
integrity.
Drug
resistance-related
transporters
such
as
ABCB1
(P-glycoprotein;
P-gp),
ABCC1
(MRP1;
multidrug
Protein
1),
and
LRP
(lung
resistance
protein)
may
MSCs
toxic
substances
chemotherapeutic
agents.
This
study
evaluated
ABCB1,
ABCC1,
before
after
differentiation
of
derived
from
human
amniotic
fluid
(AF)
bone
marrow
(BM).
P-gp
expression
in
both
AFMSCs
BMMSCs
was
analyzed
by
immunocytochemistry,
pump
function
cell
viability
assay
doxorubicin
(DOX)
Rhodamine
123
(Rh
123)
dye
exclusion.
gene
determined
RT-PCR
osteogenic
adipogenic
differentiation.
The
MES-SA/DX5
line
used
a
model
DOX
overexpression
P-gp.
Both
displayed
high
expression,
although
lower
than
control
cells.
It
shown
that
both,
undifferentiated
BMMSCs,
response
DOX,
similar
lineage.
less
expressed
BM
samples,
while
no
differences
were
observed
LRP.
showed
an
increase
differentiation,
whereas
exhibited
findings
suggest
is
influenced
processes
further
support
concept
these
modulate
MSC
source-dependent
way.
Bioengineering,
Journal Year:
2025,
Volume and Issue:
12(2), P. 120 - 120
Published: Jan. 27, 2025
The
management
of
segmental
bone
defects
presents
a
complex
reconstruction
challenge
for
orthopedic
surgeons.
Current
treatment
options
are
limited
by
efficacy
across
the
spectrum
injury,
morbidity,
and
cost.
Regional
gene
therapy
is
promising
tissue
engineering
strategy
repair,
as
it
allows
local
implantation
nucleic
acids
or
genetically
modified
cells
to
direct
specific
protein
expression.
In
cell-based
approaches,
variety
different
cell
types
have
been
described
including
mesenchymal
stem
(MSCs)
derived
from
multiple
sources—bone
marrow,
adipose,
skeletal
muscle,
umbilical
cord
tissue,
among
others.
MSCs,
in
particular,
well
studied,
they
serve
source
osteoprogenitor
addition
providing
vehicle
transgene
delivery.
Furthermore,
MSCs
possess
immunomodulatory
properties,
which
may
support
development
an
allogeneic
“off-the-shelf”
product.
Identifying
optimal
type
paramount
successful
clinical
translation
approaches.
Here,
we
review
current
strategies
loss
surgery,
grafting,
graft
substitutes,
operative
techniques.
We
also
highlight
regional
with
focus
on
sources
suitable
this
application.
Journal of Medical Virology,
Journal Year:
2025,
Volume and Issue:
97(2)
Published: Feb. 1, 2025
ABSTRACT
Influenza
A
virus
infection
can
cause
acute
respiratory
distress
syndrome
(ARDS),
and
to
date,
viral
pneumonia
has
been
the
main
of
ARDS.
Bone
marrow
mesenchymal
stem
cells
have
shown
promise
for
treating
lung
injury
caused
by
avian
influenza
infection.
At
present,
studies
use
other
cell
types
treat
human
virus‐mediated
damage
are
sparse.
We
assessed
umbilical
cord
(UC‐MSCs)
from
serious
H1N1
infections
in
animal‐based
experiments.
Maximum
titers,
inflammatory
factor
expression
levels,
differential
alveolar
cell‐related
proteins,
animal
weight
survival
rate,
histopathology,
indicators
were
evaluated.
Compared
with
control
group,
cellular
experiments,
UC‐MSCs
could
effectively
inhibit
replication
repair
damaged
host
cells.
In
reduced
pro‐inflammatory
cytokines,
entry
into
lungs,
alleviated
inflammation,
significantly
extent
mice,
improved
improving
overall
survival.
positive
role
cord‐derived
that
is
worthy
clinical
promotion
demonstrated.
Journal of Biochemical and Molecular Toxicology,
Journal Year:
2025,
Volume and Issue:
39(4)
Published: April 1, 2025
ABSTRACT
Ubiquitination
is
a
widespread
posttranslational
modification
that
plays
an
important
biological
regulatory
role
in
cells.
Research
has
reported
bone
marrow
mesenchymal
stem
cells
(BMSCs)
can
inhibit
cerebral
ischemia‐reperfusion
injury.
This
study
aims
to
explore
the
effect
of
deubiquitinating
enzymes
ubiquitin‐specific
peptidase
10
(USP10)
modified
BMSCs
exosomes
on
injury
and
underlying
mechanism.
PC12
were
stimulated
with
oxygen–glucose
deprivation/reoxygenation
(OGD/R).
The
gene
expression
was
detected
by
qRT‐PCR
western
blots.
CCK8,
EdU,
flow
cytometry
assays
conducted
assess
cell
viability,
proliferation,
apoptosis,
respectively.
Fe
2+
,
ROS,
GSH
levels
evaluate
ferroptosis.
Moreover,
identified
cytometry,
transmission
electron
microscopy.
relationship
between
USP10
solute
carrier
family
7
member
11
(SLC7A11)
confirmed
immunoprecipitation
assay.
In
addition,
rat
infarction
model
USP10‐modified
vivo.
Overexpression
alleviated
OGD/R‐induced
could
transport
USP10,
mitigated
Besides,
regulated
SLC7A11
protein
mediating
its
deubiquitination.
knockdown
restored
effects
repressed
ferroptosis
protected
against
via
deubiquitination
SLC7A11.
American Journal of Translational Research,
Journal Year:
2024,
Volume and Issue:
16(9), P. 4492 - 4503
Published: Jan. 1, 2024
To
investigate
the
role
of
Morinda
officinalis
polysaccharide
(MOP)
in
protein
expression
Wnt/β-catenin
signaling
cascade
during
osteogenic
differentiation
bone
marrow
mesenchymal
stem
cells
(BMSCs),
and
to
elucidate
mechanisms
by
which
MOP
enhances
at
cellular
level.
