Transcriptome and Proteome Analyses Revealed Differences in JEV-Infected PK-15 Cells in Response to Ferroptosis Agonists and Antagonists DOI Creative Commons
Xiaolong Zhou, Yiwei Chen, Xiangtao Kang

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(23), P. 3516 - 3516

Published: Dec. 5, 2024

Epidemic encephalitis B caused by Japanese virus (JEV) is a common zoonotic disease that poses threats to both pigs and humans. The cellular defense mechanism closely tied the body's resistance viral invasion. Regulated cell death, such as ferroptosis, strategy employed host cells defend against invasions. To understand effect of ferroptosis on proliferation JEV, experimentally infected PK15 were treated with agonist or antagonist. results indicated can suppress JEV proliferation, whereas antagonist promotes proliferation. Functional enrichment analysis showed Erastin SP600125 could affect through TNF, IL-17, Toll-like receptor, PI3K-AKT, chemokine signaling pathways, well ECM-receptor interactions. Combined transcriptome proteome analyses revealed 31 important genes, which are significantly associated inflammatory response. Our provide better understanding molecular mechanisms affects JEV.

Language: Английский

USP20 mitigates doxorubicin-induced cardiotoxicity by deubiquitinating and stabilizing HuR DOI
Zhouqing Huang, Yunxuan Chen, S S Wu

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Background The severe cardiotoxicity of doxorubicin (Dox) significantly restricts its clinical application. Deubiquitinating enzymes (DUBs) play pivotal roles in cardiac pathophysiology because their precise regulation protein function, localization and degradation.Objectives objective this study was to investigate the role molecular mechanism ubiquitin-specific peptidase 20 (USP20), a DUB, doxorubicin-induced cardiotoxicity.Methods Cardiomyocyte-specific USP20-knockout (USP20-CKO) mice were utilized assess USP20 cardiomyopathy (DIC). Coimmunoprecipitation (co-IP) combined with liquid chromatography‒mass spectrometry/mass spectrometry (LC‒MS/MS) analysis employed screen substrate USP20. Furthermore, mutant plasmids constructed elucidate underlying human antigen R (HuR) by Finally, an AAV9 vector used overexpress hearts cardiac-specific HuR-knockout interaction between HuR.Results results revealed decrease expression Dox-stimulated mouse cardiomyocytes. knockout resulted increased cardiomyocyte ferroptosis led DIC. Mechanistically, directly interacted HuR through protease structural domain. Deubiquitination at position 154 crucial for maintaining stability cleaving K48 ubiquitin chains inhibiting proteasomal degradation. Additionally, bound GPX4 mRNA suppress degradation, thereby mitigating contributing alleviating targeted overexpression via cardiomyocytes alleviated However, cardiomyocyte-specific knockout, no longer had anti-DIC effect, indicating that HuR, as downstream target USP20, plays irreplaceable DIC.Conclusions Our findings indicate enhances deubiquitination,

Language: Английский

Citations

0
Fut8 regulated Unc5b hyperfucosylation reduces macrophage emigration and accelerates atherosclerosis development via the ferroptosis pathway DOI

Rujin Liu,

Lina Dai, Shenshan Jia

et al.

Free Radical Biology and Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
VEGF-B/NRP1 Signaling Modulates Mitochondrial Homeostasis and Cardiac Function After Myocardial Infarction DOI Creative Commons

Sai Manasa Varanasi,

Ankit Sabharwal,

Riya Kar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 20, 2025

Abstract Background Myocardial infarction (MI) remains a leading cause of mortality worldwide. Recent studies suggest cardioprotective role for vascular endothelial growth factor-B (VEGF-B) in MI. However, the molecular mechanisms VEGF-B-mediated signaling via its co-receptor Neuropilin-1 (NRP1) MI are poorly understood. In this study, we investigated intricate involving VEGF-B and NRP1 cardiomyocytes (CMs) using ischemic injury as model And secondly, further validated protective VEFG-B heart disease, shedding light on their roles not only cardiac function but also therapeutic potential. Methods utilized both vitro vivo approaches to elucidate how it manifests mitochondrial functions regeneration following injury. We used two different cardiomyocyte cell lines, H9c2 (rat ventricular cardiomyocytes) HL-1 (mouse cardiomyocytes), induced hypoxia conditions, either 1% oxygen or 200µM cobalt chloride (CoCl 2 ) mimic myocardial infarction-induced heart. addition, developed novel heat shock inducible zebrafish cardiomyocyte-specific overexpression system examine . Results Our findings indicate that predominantly expressed tissue compared other tissues, expression is altered response hypoxia/ischemic results demonstrate treatment prior enhances survival, while knockdown abolishes effect, highlighting prominent cardio protection. Furthermore, found promotes survival by improving function, evidenced reduced oxidative stress ROS accumulation, decreased stress, preserved membrane potential, increased ATP levels. Lastly, our transgenic model, demonstrated protects from an NRP1-dependent manner. Conclusion study has uncovered important VEGF-B-NRP1 axis mediated beneficial CMs. Importantly, against Novelty Significance What Is Known? dysfunction, death, adverse remodeling. Vascular Endothelial Growth Factor B traditionally associated with metabolism, biology, particularly angiogenesis lipid metabolism. The neurophilin-1 receptor, VEGF-B, implicated pathways. Emerging evidence suggests may influence integrity under conditions. New Information Does This Article Contribute? exerts effect mitocondria preserving reducing stress. receptor key mediator modulates homeostasis cardiomyocytes. can attenuate apoptosis enhance bioenergetics post-MI, suggesting mechanism. Clinical Implication: These strategies aimed at enhancing VEGF-B/NRP1 improve recovery after MI, offering avenue limiting failure progression disease.

Language: Английский

Citations

0
From bench to bedside: targeting ferroptosis and mitochondrial damage in the treatment of diabetic cardiomyopathy DOI Creative Commons
Bin Liu, Qing Jin, Yi Sun

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: April 25, 2025

Diabetic cardiomyopathy (DCM) is a common and fatal cardiac complication caused by diabetes, with its pathogenesis involving various forms of cell death mitochondrial dysfunction, particularly ferroptosis injury. Recent studies have indicated that damage play crucial roles in the onset progression DCM, though their precise regulatory mechanisms remain unclear. Of particular interest interaction between damage, as well synergistic effects, which are not fully understood. This review summarizes injury DCM explores molecular involved, an emphasis on interplay these two processes. Additionally, article offers overview targeted drugs shown to be effective cellular experiments, animal models, clinical trials, analyzing action potential side effects. The goal provide insights for future drug development applications. Moreover, challenges prospects multi-target combination therapies personalized medicine interventions practice offer strategic guidance comprehensive prevention management DCM.

Language: Английский

Citations

0
GPX4 reverses the effect of USP31 depletion on radiosensitivity and ferroptosis USP31 confers radio-resistance in cervical cancer cells via ferroptosis pathway by deubiquitinating and stabilizing GPX4 DOI
Qi Ding, Yan Zhuang, Hongyu Yang

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: 770, P. 151990 - 151990

Published: May 9, 2025

Language: Английский

Citations

0
Mechanistic role of environmental toxicants in inducing cellular ferroptosis and its associated diseases DOI
Hong Chen,

Bingchun Liu,

Peixin Xu

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 298, P. 118269 - 118269

Published: May 10, 2025

Language: Английский

Citations

0
The multifaceted perspectives on the regulation of lncRNAs in hepatocellular carcinoma ferroptosis: from bench-to-bedside DOI Creative Commons
Xin Jin,

C. Huang,

Yue Tian

et al.

Clinical and Experimental Medicine, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 3, 2024

Despite being characterized by high malignancy, morbidity, and low survival rates, the underlying mechanism of hepatocellular carcinoma (HCC) has not been fully elucidated. Ferroptosis, a non-apoptotic form regulated cell death, possesses distinct morphological, biochemical, genetic characteristics compared to other types death. Dysregulated actions within molecular network that regulates ferroptosis have identified as significant contributors progression HCC. Long non-coding RNAs (lncRNAs) emerged influential diverse cellular processes, regulating gene function expression through multiple mechanistic pathways. An increasing body evidence indicates deregulated lncRNAs are implicated in malignant events such proliferation, growth, invasion, metabolism influencing Therefore, elucidating inherent role modulatory functions on HCC might promote development novel therapeutic interventions for this disease. This review provides succinct overview roles ferroptosis-related treatment, aiming drive promising targets biomarkers patients.

Language: Английский

Citations

2
La nueva guía sobre los puntos de corte de la hemoglobina para definir anemia en individuos y poblaciones DOI Creative Commons

Cinthya Vásquez-Velásquez,

Vilma Tapia, Gustavo F. Gonzáles

et al.

Boletín de la Sociedad Peruana de Medicina Interna/Revista de la Sociedad Peruana de Medicina Interna, Journal Year: 2024, Volume and Issue: 37(1), P. 15 - 20

Published: April 5, 2024

En el presente año, la Organización Mundial de Salud (OMS) ha establecido una nueva guía indicando un reajuste los puntos corte para diagnóstico anemia en infantes 6 a 23 meses, así como, cambio del factor corrección hemoglobina (Hb) por altura residencia, cual genera panorama distinto sobre las cifras reportadas encuestas previas aplicadas nivel nacional. Lima Metropolitana, reanálisis bases datos SIEN (CENAN/INS) 2012 al 2017 muestra que prevalencia se reduce 45.4% 25.5% con nuevas guías OMS. Por otra parte, existe vacío conocimiento efectividad y repercusiones han generado diversas estrategias aplican territorio peruano, además algunos países vecinos tienen gran proporción residentes altura. Debido lo antes mencionado, manuscrito tiene como objetivo evidenciar cambios presentado largo años prevalencias infantil, mediante línea tiempo analizar nacionales e internacionales.

Citations

1
Transcriptomic insights into pseudorabies virus suppressed cell death pathways in neuroblastoma cells DOI Creative Commons
Shinuo Cao, Li Zhang, Mo Zhou

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 19, 2024

Pseudorabies virus (PRV) exhibits a complex interplay of host-pathogen interactions, primarily by modulating host cell death pathways to optimize its replication and spread in Neuro-2a cells. Using high-throughput RNA sequencing, we identified 2,382 upregulated differentially expressed genes (DEGs) 3,998 downregulated DEGs, indicating intricate interaction between viral pathogenesis cellular responses. This research offers valuable insights into the molecular processes involved PRV infection, highlighting substantial inhibition crucial cells, including necroptosis, pyroptosis, autophagy, ferroptosis, cuproptosis. Cells infected with exhibit decreased expression critical these pathways, potentially as mechanism avoid immune reactions ensure survival support ongoing replication. extensive apoptosis metabolic alterations highlights sophisticated tactics utilized PRV, enhancing our comprehension herpesvirus biology feasibility creating specific antiviral treatments. contributes understanding how viruses manipulate presents potential opportunities for therapeutic interventions disrupt virus’s lifecycle.

Language: Английский

Citations

1
MambaVesselNet: A Novel Approach to Blood Vessel Segmentation Based on State-Space Models DOI
Tianyong Liu, Zhiqing Zhang,

Guojia Fan

et al.

IEEE Journal of Biomedical and Health Informatics, Journal Year: 2024, Volume and Issue: 29(3), P. 2034 - 2047

Published: Dec. 18, 2024

Three-dimensional blood vessel segmentation is an important and challenging task that faces two main difficulties: (1) structures are small, making them hard to capture by the network, edges difficult segment accurately; (2) false positives prone occur due presence of artifacts noise. This paper proposes a novel method called MambaVesselNet. based on state-space model employs selective time series modeling strategy achieve larger receptive field. To better fine vascular accurately edges, this introduces edge enhancement module feature selection module. In terms data preprocessing, nnUNet's preprocessing adopted ensure spatial consistency input data. Evaluation three standard benchmarks shows MambaVesselNet achieves state-of-the-art performance. Specifically, cardiovascular liver datasets, Dice coefficient improved 1.38% 2.69%, respectively. The contributions include proposal new for enhancing features, development with long sequence capability, adoption strategy, setting benchmark technology.

Language: Английский

Citations

1