Performance of Trichogramma japonicum under field conditions as a function of the factitious host species used for mass rearing

PLoS ONE, Journal Year: 2021, Volume and Issue: 16(8), P. e0256246 - e0256246

Published: Aug. 19, 2021

Different factitious hosts were used to mass rear Trichogramma japonicum Ashmead in different parts of the globe because thorough details lacking both laboratory and field. The objective this study was compare, parasitoid, T . reared hosts. Three commonly host eggs, Corcyra cephalonica (Stainton), Ephestia kuehniella Zeller Sitotroga cerealella Olivier tested under conditions then field over a yellow stem borer, Scirpophaga incertulus (Walker) rice. highest parasitism by observed on E eggs. parasitoid’s emergence (88.99%) S eggs at 24 h exposure, whereas 48 it (94.66%). females that emerged from significantly long-lived. days oviposition species significant individually, but not their interaction. Higher proportions flying when C Field results showed mass-reared higher its natural host, Hence, considering these biological characteristics results, could be leveraged for rearing quality parasitoids India, Asian continent beyond.

Language: Английский

---

Down-Regulation of P450 Genes Enhances Susceptibility to Indoxacarb and Alters Physiology and Development of Fall Armyworm, Spodoptera frugipreda (Lepidoptera: Noctuidae)

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13

Published: May 9, 2022

The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), is a pest of many important crops globally. Effective control challenging, with the exhibiting resistance to different synthetic pesticides across various groups. However, mechanisms employed by resistant insects for overexpression relevant detoxification genes remain unclear. activity enzymes was investigated in this study. Additionally, using RNA interference (RNAi), functional analysis completed two P450s an indoxacarb population armyworms. Elevated levels (resistance ratio = 31.37-fold) indoxacarb-selected populations FAW were observed after 14 generations. qRT-PCR showed higher expression cytochrome P450 genes, CYP321A7 and CYP6AE43, selected compared population. RNAi applied knock down dsCYP321A7 dsCYP6AE43 larvae. Droplet feeding dsRNAs (CYP321A7 CYP6AE43) via artificial diet significantly increased mortality rates treated A shorter larval developmental time detected all dsRNAs-fed Correspondingly, mass reduced armyworm. Larval assays demonstrate that targeting, specifically CYP6AE43 enzymes, could be beneficial technique management populations. Further study on potential use dsRNA its application should conducted efforts counter development against insecticides field.

Language: Английский

---

Comparative low lethal effects of three insecticides on demographical traits and enzyme activity of the Spodoptera exigua (Hübner)

Environmental Science and Pollution Research, Journal Year: 2022, Volume and Issue: 29(40), P. 60198 - 60211

Published: April 13, 2022

Language: Английский

---

Sublethal concentrations of thiamethoxam induce transgenerational hormesis in cotton aphid, Aphis gossypii Glover

CABI Agriculture and Bioscience, Journal Year: 2023, Volume and Issue: 4(1)

Published: Nov. 20, 2023

Abstract In agroecosystems, insects have to compete with chemical insecticides, which are frequently present at sublethal concentrations. The exposure of these modest stresses is now well-established generate hormesis effects, has implications for controlling insect pests. this study, we assessed the effects thiamethoxam on biological parameters Aphis gossypii Glover (Hemiptera: Aphididae), adults (F 0 ) and subsequent transgenerational impacts, i.e., progeny 1 generation), using an age stage, two-sex life table analysis. Results showed that exhibited high toxicity against adult A. LC 50 0.313 mg L −1 after 48 h exposure. 5 10 considerably reduced cotton aphids longevity fecundity, while reproductive days were only . pre-adult stage was decreased, longevity, total fecundity significantly extended in F concentrations thiamethoxam. Moreover, key demographic such as intrinsic rate increase, finite increase increased, mean generation time prereproductive progeny. No significant observed net rate. Taken together, results affect directly exposed causing hormetic conclusion, our research both aphids; it could be effective IPM programs targeting pest.

Language: Английский

---

Transcription Factors AhR and ARNT Regulate the Expression of CYP6SX1 and CYP3828A1 Involved in Insecticide Detoxification in Bradysia odoriphaga

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(19), P. 10805 - 10813

Published: May 7, 2024

Aryl hydrocarbon receptor (AhR) and aryl nuclear translocator (ARNT) mediate the responses of adaptive metabolism to various xenobiotics. Here, we found that

Language: Английский

---

Characterization of two glutathione S‐transferase genes involved in clothianidin resistance in Bradysia odoriphaga

Pest Management Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Glutathione S-transferase (GST) is a key phase II detoxification enzyme involved in xenobiotics metabolism, and plays pivotal role the evolution of resistance to various types insecticides. However, specific functions GST genes clothianidin remain obscure Bradysia odoriphaga.

Language: Английский

---

Sub-Lethal Effects of Pirimiphos-Methyl Are Expressed to Different Levels in Wings of Three Stored-Product Coleopterans: A Geometric Morphometrics Investigation

Insects, Journal Year: 2023, Volume and Issue: 14(5), P. 430 - 430

Published: April 30, 2023

Tenebrio molitor L. (Coleoptera: Tenebrionidae), Prostephanus truncatus (Horn), and Rhyzopertha dominica (F.) Bostrychidae) are noxious insect pests of grains in storages. Pirimiphos-methyl is widely used to protect at the post-harvest stage. However, sub-lethal impact this active ingredient on offspring all three coleopterans remains unknown. Thus, mated females each species were exposed separately pirimiphos-methyl short exposures (30 min, 3, 5, 8, 16, 24, 36 h), where elytra hindwings adult progeny analyzed with geometric morphometrics method. Males incorporated into analysis. The results revealed variability among species. was most sensitive species, displaying significant deformations hindwings. had more conspicuous morphological changes than females. exhibited deformities after h exposure pirimiphos-methyl. In contrast, R. not affected by light our findings, organophosphorus insecticides may cause variable effects stored-product insects. This issue lead different insecticidal treatments according targeted

Language: Английский

---

DIMBOA-induced gene expression, activity profiles of detoxification enzymes, multi-resistance mechanisms, and increased resistance to indoxacarb in tobacco cutworm, Spodoptera litura (Fabricius)

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 267, P. 115669 - 115669

Published: Nov. 1, 2023

Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) is one of the most destructive insect pests owned strong resistance to different insecticides. Indoxacarb as a novel oxadiazine insecticide becomes main pesticide against S. litura. DIMBOA [2,4-dihydroxy-7-methoxy-2 H-1,4-benz-oxazin-3(4 H)-one] involved in important chemical defense processes corn plants. However, insects' adaptation mechanism insecticides when exposed defensive allelochemicals their host plants remains unclear. Here, we assessed multi-resistance, and mechanisms based on life history traits. After 18 generations selection, indoxacarb was increased by 61.95-fold (Ind-Sel) 86.06-fold (Dim-Sel) compared Lab-Sus. Also, DIMBOA-pretreated larvae developed high beta-cypermethrin, chlorpyrifos, phoxim, chlorantraniliprole, emamectin benzoate. Meanwhile, (LC50) applied detect its impact thirty-eight detoxification-related genes expression. The transcripts SlituCOE073, SlituCOE009, SlituCOE074, SlituCOE111 well SlGSTs5, SlGSTu1, SlGSTe13 were considerably raised Ind-Sel strain. Among twenty-three P450s, CYP6AE68, CYP321B1, CYP6B50, CYP9A39, CYP4L10, CYP4S9v1 denoted significantly higher levels strain, suggesting that CarEs, GSTs P450s may be engaged resistance. These outcomes further highlighted importance detoxification enzymes for gene expression role responses pest management approaches.

Language: Английский

---

Sublethal and Transgenerational Toxicities of Chlorfenapyr on Biological Traits and Enzyme Activities of Paracoccus marginatus (Hemiptera: Pseudococcidae)

Insects, Journal Year: 2022, Volume and Issue: 13(10), P. 874 - 874

Published: Sept. 26, 2022

Papaya mealybug, Paracoccus marginatus Williams and Granara de Willink (Hemiptera: Pseudococcidae), is an economically important, invasive insect that now distributed worldwide. Chlorfenapyr has been demonstrated to have a significant control effect on P. marginatus. In order evaluate the sublethal transgenerational effects of chlorfenapyr marginatus, life table data three consecutive generations were collected analyzed by age stage, two-sex method, enzyme activities assayed using spectrophotometer. The results showed exposure insecticide had biological traits subsequent higher intrinsic rate increase (r), finite (λ), net reproductive (R0), shorter mean generation time (T) observed in chlorfenapyr-treated F1 mealybugs. Enzyme activity assays significantly inhibited catalase (CAT) peroxidase (POD) while activating superoxide dismutase (SOD), which suggested SOD, CAT, POD may play important role self-defense against chlorfenapyr. These conclusively concentrations induced hormetic having negative F0 F3 generations.

Language: Английский

---

Residue, Dissipation Pattern, and Dietary Risk Assessment of Imidacloprid in Chinese Chives

Frontiers in Nutrition, Journal Year: 2022, Volume and Issue: 9

Published: Feb. 23, 2022

The demand for Chinese chives is growing as they are also rich in vitamins, fiber, and sulfur nutrients. should be sprayed with imidacloprid to control pests diseases safeguard their yield meet the demands of East Asian consumers chives. Overspraying can lead residues chives, posing a severe risk human health. To reduce harmful effects on humans, we investigated dissipation pattern final residue using quick, easy, cheap, effective, rugged, safe (QuEChERS) method combined liquid chromatography-tandem mass spectrometry (LC-MS/MS). Good linearity (R2= 0.9988), accuracy (expressed recovery % 78.34-91.17%), precision [expressed relative SDs (RSDs) 0.48-6.43%], sensitivity [a limit quantification (LOQ) ≤ 8.07 × 104 mg/kg] were achieved. dynamics consistent first-order kinetics, half-life 2.92 days. residual levels 0.00923-0.166 mg/kg, which lower than maximum limits (MRLs) 1 mg/kg A assessment index <1 indicates that consumption.

Language: Английский

---