Major alteration of lung microbiome and the host responses in critically ill COVID-19 patients with high viral load DOI Creative Commons
Ingrid G. Bustos, Rosana Wiscovitch-Russo, Harinder Singh

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 12, 2024

Patients with COVID-19 under invasive mechanical ventilation are at higher risk of developing ventilator-associated pneumonia (VAP), associated increased healthcare costs, and unfavorable prognosis. The underlying mechanisms this phenomenon have not been thoroughly dissected. Therefore, study attempted to bridge gap by performing a lung microbiota analysis evaluating the host immune responses that could drive development VAP. In prospective cohort study, mechanically ventilated patients confirmed SARS-CoV-2 infection were enrolled. Nasal swabs (NS), endotracheal aspirates (ETA), blood samples collected initially within 12 h intubation again 72 post-intubation. Plasma underwent cytokine metabolomic analyses, while NS ETA sequenced for microbiome examination. was categorized based on Data conducted using RStudio version 4.3.1. 36 ventilation, significant differences found in nasal pulmonary microbiome, notably Staphylococcus Enterobacteriaceae, linked VAP showed viral load respiratory samples, elevated neutralizing antibodies, reduced inflammatory cytokines, including IFN-δ, IL-1β, IL-12p70, IL-18, IL-6, TNF-α, CCL4. Metabolomic revealed changes 22 metabolites non-VAP 27 patients, highlighting D-Maltose-Lactose, Histidinyl-Glycine, various phosphatidylcholines, indicating metabolic predisposition This reveals critical link between alterations loads These findings provide novel insights into VAP, potential implications management prevention.

Language: Английский

Mesenchymal stromal cells derived from various tissues: Biological, clinical and cryopreservation aspects: Update from 2015 review DOI Creative Commons
Leah A. Marquez‐Curtis,

Janet A.W. Elliott

Cryobiology, Journal Year: 2024, Volume and Issue: 115, P. 104856 - 104856

Published: Feb. 8, 2024

Mesenchymal stromal cells (MSCs) have become one of the most investigated and applied for cellular therapy regenerative medicine. In this update our review published in 2015, we show that studies continue to abound regarding characterization MSCs distinguish them from other similar cell types, discovery new tissue sources MSCs, confirmation their properties functions render suitable as a therapeutic. Because cryopreservation is widely recognized only technology would enable on-demand availability here although traditional method cryopreserving by slow cooling presence 10% dimethyl sulfoxide (Me2SO) continues be used many, several novel MSC approaches emerged. As previous review, conclude these recent reports viable functional diverse tissues can recovered after using variety cryoprotectants, freezing protocols, storage temperatures, periods storage. We also logistical reasons there are now more devoted which derived. A topic included covers application COVID-19 arising immunomodulatory antiviral properties. Due inherent heterogeneity populations different still no standardized procedure isolation, identification, characterization, cryopreservation, route administration, not likely "one-size-fits-all" approach applications cell-based

Language: Английский

Citations

5
Revisiting the potential of natural antimicrobial peptides against emerging respiratory viral disease: a review DOI Creative Commons

Neelakanta Sarvashiva Kiran,

Sudarshan Singh,

Chandrashekar Yashaswini

et al.

3 Biotech, Journal Year: 2025, Volume and Issue: 15(2)

Published: Jan. 13, 2025

Language: Английский

Citations

0
Differential responses of lung and intestinal microbiota to SARS-CoV-2 infection: a comparative study of the Wuhan and Omicron strains in K18-hACE2 Tg mice DOI Creative Commons
Chae Won Kim, Keun Bon Ku, Insu Hwang

et al.

Laboratory Animal Research, Journal Year: 2025, Volume and Issue: 41(1)

Published: April 23, 2025

Abstract Background The COVID-19 pandemic, caused by SARS-CoV-2, has led to the emergence of viral variants with distinct characteristics. Understanding differential impacts SARS-CoV-2 is crucial for effective public health response and treatment development. We investigated effects original Wuhan strain emergent Omicron variant using a K18-hACE2 transgenic mouse model. compared mortality rates, loads, histopathological changes in lung tracheal tissues, as well alterations intestinal microbiota following infection. Results Our findings revealed significant differences between variants, causing higher severe pathology, elevated loads variant. Microbiome analyses uncovered novel shifts associated each variant, providing evidence variant-specific microbiome alterations. These suggest microbiome-related mechanisms that might modulate disease severity host responses Conclusions This study highlights critical terms mortality, changes, emphasizing role influencing outcomes. Novel include identification shifts, which underscore potential underlying severity. insights pave way future research exploring microbiome-targeted interventions mitigate other infections.

Language: Английский

Citations

0
Intestinal Damage, Inflammation and Microbiota Alteration during COVID-19 Infection DOI Creative Commons
Angela Saviano, Mattia Brigida, Carmine Petruzziello

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(4), P. 1014 - 1014

Published: March 27, 2023

Background: The virus SARS-CoV-2 is responsible for respiratory disorders due to the fact that it mainly infects tract using Angiotensin-converting enzyme 2 (ACE2) receptors. ACE2 receptors are also highly expressed on intestinal cells, representing an important site of entry in gut. Literature studies underlined and replicates gut epithelial causing gastrointestinal symptoms such as diarrhea, abdominal pain, nausea/vomiting anorexia. Moreover, settles into bloodstream, hyperactivating platelets cytokine storms gut–blood barrier damage with alteration microbiota, cell injury, vessel thrombosis leading malabsorption, malnutrition, increasing disease severity mortality short long-period sequelae. Conclusion: This review summarizes data how effects systems, including mechanisms inflammation, relationship endoscopic patterns, role fecal calprotectin, confirming importance digestive system clinical practice diagnosis follow-up infection.

Language: Английский

Citations

8
Development and validation of machine learning-based models for predicting healthcare-associated bacterial/fungal infections among COVID-19 inpatients: a retrospective cohort study DOI Creative Commons
Min Wang, Wenjuan Li, Hui Wang

et al.

Antimicrobial Resistance and Infection Control, Journal Year: 2024, Volume and Issue: 13(1)

Published: April 14, 2024

Abstract Background COVID-19 and bacterial/fungal coinfections have posed significant challenges to human health. However, there is a lack of good tools for predicting coinfection risk aid clinical work. Objective We aimed investigate the factors among patients develop machine learning models estimate coinfection. Methods In this retrospective cohort study, we enrolled adult inpatients confirmed with in tertiary hospital between January 1 July 31, 2023, China collected baseline information at admission. All data were randomly divided into training set testing ratio 7:3. developed generalized linear random forest assessed performance set. Decision curve analysis was performed evaluate applicability. Results A total 1244 included 62 healthcare-associated infections, while 534 22 infections. found that comorbidities (diabetes, neurological disease) greater than those without (OR = 2.78, 95%CI 1.61–4.86; OR 1.93, 1.11–3.35). An indwelling central venous catheter or urinary also associated an increased 2.53, 1.39–4.64; 2.28, 1.24–4.27) coinfections. Patients PCT > 0.5 ng/ml 2.03 times (95%CI 1.41–3.82) more likely be infected. Interestingly, IL-6 concentration < 10 pg/ml 1.69, 0.97–2.94). low creatinine levels had decreased coinfections(OR 0.40, 0.22–0.71). The demonstrated favorable receiver operating characteristic curves (ROC 0.87, 0.80–0.94; ROC 0.88, 0.82–0.93) high accuracy, sensitivity specificity 0.86vs0.75, 0.82vs0.86, 0.87vs0.74, respectively. corresponding calibration evaluation P statistics 0.883 0.769. Conclusions Our achieved strong predictive ability may effective decision-support identifying guiding antibiotic administration. cytokines, such as IL-6, affect status

Language: Английский

Citations

2
"Multidrug-Resistant Bacteria Related to Covid-19 Infection: A Minireview" DOI Open Access
José Alexandre da Rocha Curvelo

Biomedical Journal of Scientific & Technical Research, Journal Year: 2024, Volume and Issue: 55(3)

Published: March 5, 2024

The World Health Organization recorded antimicrobial multidrug resistance as one of the biggest health risks in 2019. inappropriate use antibiotics results their consequent ineffectiveness treatment bacterial infections, hindering therapeutic alternatives and increasing risk poor prognosis. In late 2019, a single-stranded RNA zoonotic virus, SARS-CoV-2, was identified caused Coronavirus Disease-19 pandemic. prophylactically for secondary infections measures taken emergency scenario.

Language: Английский

Citations

1
Differential Responses of Lung and Intestinal Microbiota to SARS-CoV-2 Infection: A Comparative Study of the Wuhan and Omicron Strains in K18-hACE2 tg Mice DOI Creative Commons
Chae Won Kim, Keun Bon Ku, Insu Hwang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

Abstract The COVID-19 pandemic, caused by SARS-CoV-2, has led to the emergence of viral variants with distinct characteristics. We investigated differential effects original Wuhan strain and emergent Omicron variant SARS-CoV-2 using a K18-hACE2 transgenic mouse model. compared mortality rates, loads, histopathological changes in lung tracheal tissues, as well alterations intestinal microbiota following infection. observed significant differences disease severity, causing higher more severe damage than variant. Furthermore, microbiome analyses revealed shifts associated infection each variant, suggesting that microbiome-related mechanisms might influence outcomes. This comprehensive comparison enhances our understanding pathogenesis highlights importance dynamics infections, providing insights for future therapeutic preventive strategies. Importance Understanding impacts is crucial effective public health response treatment development. study provides into revealing host mortality, load, pathology. use model enables detailed examination these controlled setting. this modulating severity responses infections. By uncovering microbial different variants, suggests potential be targeted mitigate

Language: Английский

Citations

0
The impact of SARS-CoV-2 infection at different stages of pregnancy on placental inflammatory responses DOI Creative Commons
Mengyue Yin, Lingling Peng,

Chunling Zhang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Abstract Background The specific impact and pathology of SARS-CoV-2 infection on maternal fetal health have not been comprehensively investigated. Therefore, we investigated the inflammatory response tissues in pregnant women infected with at different stages pregnancy. Methods We collected placenta samples from 52 patients Tai’an Central Hospital, who underwent delivery between November 2022 September 2024. analyzed general patient data maternal-fetal outcomes conducted histological observations using HE staining. Furthermore, used ELISA to quantitatively analyze concentration IL-6 umbilical cord blood amniotic fluid. Additionally, Western blot analysis was evaluate expression levels TNF-α IL-1β placental tissues. Results Among participants, 33 were diagnosed infection. Patients during mid-pregnancy developed thrombosis, stromal protein deposition, villous interstitial inflammation compared control group (P < 0.05). However, No significant differences found late-term > revealed elevated tissues, no difference mid-term pregnancies. fluid showed difference, detected blood. Conclusion pregnancy appears limited overall outcomes. may lead changes particularly mid-trimester. These findings suggest importance careful monitoring management women. Nonetheless, larger studies are necessary confirm these explore long-term effects both health.

Language: Английский

Citations

0
Major alteration of lung microbiome and the host responses in critically ill COVID-19 patients with high viral load DOI Creative Commons
Ingrid G. Bustos, Rosana Wiscovitch-Russo, Harinder Singh

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 12, 2024

Patients with COVID-19 under invasive mechanical ventilation are at higher risk of developing ventilator-associated pneumonia (VAP), associated increased healthcare costs, and unfavorable prognosis. The underlying mechanisms this phenomenon have not been thoroughly dissected. Therefore, study attempted to bridge gap by performing a lung microbiota analysis evaluating the host immune responses that could drive development VAP. In prospective cohort study, mechanically ventilated patients confirmed SARS-CoV-2 infection were enrolled. Nasal swabs (NS), endotracheal aspirates (ETA), blood samples collected initially within 12 h intubation again 72 post-intubation. Plasma underwent cytokine metabolomic analyses, while NS ETA sequenced for microbiome examination. was categorized based on Data conducted using RStudio version 4.3.1. 36 ventilation, significant differences found in nasal pulmonary microbiome, notably Staphylococcus Enterobacteriaceae, linked VAP showed viral load respiratory samples, elevated neutralizing antibodies, reduced inflammatory cytokines, including IFN-δ, IL-1β, IL-12p70, IL-18, IL-6, TNF-α, CCL4. Metabolomic revealed changes 22 metabolites non-VAP 27 patients, highlighting D-Maltose-Lactose, Histidinyl-Glycine, various phosphatidylcholines, indicating metabolic predisposition This reveals critical link between alterations loads These findings provide novel insights into VAP, potential implications management prevention.

Language: Английский

Citations

0