Multiomics insights into the female reproductive aging

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 95, P. 102245 - 102245

Published: Feb. 23, 2024

The human female reproductive lifespan significantly diminishes with age, leading to decreased fertility, reduced fertility quality and endocrine function disorders. While many aspects of aging in general have been extensively documented, the precise mechanisms governing programmed system remain elusive. Recent advancements omics technologies computational capabilities facilitated emergence multiomics deep phenotyping. Through application refinement various high-throughput methods, a substantial volume data has generated, deepening our comprehension pathogenesis molecular underpinnings aging. This review highlights current emerging approaches for investigating aging, encompassing genomics, epigenomics, transcriptomics, proteomics, metabolomics, microbiomics. We elucidate their influence on fundamental cell biology translational research context address limitations challenges associated studies, offer glimpse into future prospects.

Language: Английский

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Analysis of single-cell RNA sequencing in human oocytes with diminished ovarian reserve uncovers mitochondrial dysregulation and translation deficiency

Reproductive Biology and Endocrinology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 15, 2024

Diminished ovarian reserve (DOR) is clinically characterized by a decrease in the number of available follicles and decline quality oocytes, accompanied hormonal changes. Low DOR oocyte leads to impaired embryo development, an increased risk aneuploid pregnancies miscarriages. However, specific pathogenic mechanism remains unclear, posing significant challenge for assisted reproductive technology. For first time, our study employed single-cell RNA sequencing reveal altered transcriptomic landscape oocytes at GV stage after stimulation. Differentially expressed genes analysis (DEGs), functional enrichment analysis, weighted gene co-expression network (WGCNA) protein-protein interactions were performed. We found 132 up-regulated 466 down-regulated with primarily enriched mitochondrial function translation. Hub genes, identified through integrated WGCNA DEGs, further validated control using RT-qPCR. By utilizing hub employing transcription factor tools, it had been predicted that pleomorphic adenoma 1 (PLAG1) played crucial role as transcriptional regulatory oocytes. Additionally, we conformed PLAG1-IGF2 axis was dysregulated Transcriptome revealed exhibited dysfunction translational defects, might be potential contributor low

Language: Английский

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Chromosome Segregation–1–like Gene Participates in Ferroptosis in Human Ovarian Granulosa Cells via Nucleocytoplasmic Transport

Antioxidants, Journal Year: 2024, Volume and Issue: 13(8), P. 911 - 911

Published: July 28, 2024

Premature ovarian insufficiency (POI) is defined as the depletion of function before age 40 years. The global prevalence POI 3.5%. To date, genetic factors account for 23.5% etiology POI. Herein, a previously uncharacterized pathogenic homozygous variant chromosome segregation-1-like gene (CSE1L) was identified in patients via targeted panel sequencing. It reported that dysregulated iron metabolism involved many reproductive endocrine disorders; however, its precise role remains obscure. In this study, we CSE1L potential candidate plays an important maintaining homeostasis. Deficiency led to ferroptosis human granulosa cells, which confirmed by transmission electron microscopy. Mechanistically, coimmunoprecipitation direct interaction between and FoxO1. Inhibition excessive accumulation FoxO1 nucleus nucleocytoplasmic transport. Then, bound promoter region NCOA4 promoted transcription, verified chromatin immunoprecipitation assay. Moreover, inhibition cumulus cell monolayer could impede oocyte maturation, might be associated with oxidative stress. Consequently, our study first revealed participated cells transportation, helpful revealing molecular mechanism development Importantly, these findings provide new insights into application inhibitors treatment

Language: Английский

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Resolving and functional analysis of RNA editing sites in sheep ovaries and associations with litter size

animal, Journal Year: 2024, Volume and Issue: 18(11), P. 101342 - 101342

Published: Sept. 24, 2024

Language: Английский

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