Small,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 4, 2024
Abstract
The
gut‐bone
axis
is
a
promising
target
for
osteoporosis
treatment,
yet
existing
delivery
systems
lack
precise
targeting.
Herein,
an
oral
hydrogel
microsphere
system
(E7‐Lipo@Alg/Cs)
developed
using
gas
microfluidic
and
ionic
crosslinking
technologies
to
deliver
drugs
bone
marrow
mesenchymal
stem
cells
(BMSCs)
via
the
axis,
regulating
mitochondrial
aging.
A
BMSC‐affine
peptide
conjugated
onto
liposomes
encapsulating
Fisetin,
followed
by
incorporation
into
alginate‐calcium
microspheres.
Chitosan
electrostatically
adsorbed
surface,
creating
core‐shell
structure
that
adheres
intestinal
epithelial
cells,
withstands
gastric
acid,
facilitates
targeted
BMSCs
through
intestinal‐bone
axis.
In
vitro,
effectively
enhances
function
reverses
BMSC
aging,
while
in
vivo
studies
demonstrate
prolonged
drug
activity,
restored
osteogenic
differentiation,
regeneration.
RNA‐seq
indicates
activation
of
AMPK‐SIRT1
pathway,
reversing
aging
promoting
aged
tissue
This
provides
efficient
strategy
preventing
loss,
offering
significant
clinical
potential
treatment.
Subcellular
mitochondria
serve
as
sensors
for
energy
metabolism
and
redox
balance,
the
dynamic
regulation
of
functional
dysfunctional
plays
a
crucial
role
in
determining
cells'
fate.
Selective
removal
at
subcellular
level
can
provide
chondrocytes
with
to
prevent
degeneration,
thereby
treating
osteoarthritis.
Herein,
achieve
an
ideal
therapy,
cartilage
affinity
peptide
(WYRGRL)-decorated
liposomes
loaded
mitophagy
activator
(urolithin
A)
were
integrated
into
hyaluronic
acid
methacrylate
hydrogel
microspheres
through
microfluidic
technology,
named
HM@WY-Lip/UA,
that
could
efficiently
target
selectively
remove
mitochondria.
As
result,
this
system
demonstrated
advantage
function
restoration,
reactive
oxygen
species
scavenging,
cell
survival
rescue,
chondrocyte
homeostasis
maintenance
increasing
mitophagy.
In
rat
post-traumatic
osteoarthritis
model,
intra-articular
injection
HM@WY-Lip/UA
ameliorated
matrix
degradation,
osteophyte
formation,
subchondral
bone
sclerosis
8
weeks.
Overall,
study
indicated
provided
protective
effect
on
degeneration
efficacious
clinically
relevant
manner,
mitochondrial-oriented
strategy
has
great
potential
therapy
Journal of Translational Medicine,
Journal Year:
2025,
Volume and Issue:
23(1)
Published: Jan. 7, 2025
The
inflammatory
responses
from
synovial
fibroblasts
and
macrophages
the
mitochondrial
dysfunction
in
chondrocytes
lead
to
oxidative
stress,
disrupt
extracellular
matrix
(ECM)
homeostasis,
accelerate
deterioration
process
of
articular
cartilage
osteoarthritis
(OA).
In
recent
years,
it
has
been
proposed
that
mesenchymal
stromal
cells
(MSC)
transfer
their
functional
mitochondria
damaged
response
cellular
becoming
one
mechanisms
underpinning
therapeutic
effects.
Therefore,
we
hypothesize
a
novel
cell-free
treatment
for
OA
could
involve
direct
transplantation,
restoring
both
homeostasis.
Mitochondria
were
isolated
Umbilical
Cord
(UC)-MSC
(Mito-MSC)
characterized
based
on
morphology,
phenotype,
functions,
ability
be
internalized
by
different
cells.
Furthermore,
transcriptional
changes
following
uptake
evaluated
using
an
Affymetrix
analysis,
Lastly,
dose
dependence
efficacy,
biodistribution
immunogenicity
Mito-MSC
assessed
vivo,
through
intra-articular
injection
male
C57BL6
mice
collagenase-induced
(CIOA)
model.
Our
findings
demonstrate
integrity
efficiently
transferred
into
chondrocytes,
macrophages,
fibroblasts.
Moreover,
transcriptomic
analysis
showed
upregulation
genes
involved
stress
such
as
DNA
reparative
machinery
antiviral
responses.
Finally,
transplantation
yielded
significant
reductions
joint
mineralization,
hallmark
progression,
well
improvements
OA-related
histological
signs,
with
lower
exhibiting
better
efficacy.
was
detected
within
knee
up
24
h
post-injection
without
eliciting
CIOA
mice.
Collectively,
our
results
reveal
derived
MSC
are
key
retained
generating
immune
mitigating
degradation
OA,
probably
restorative
effect
triggered
chondrocytes.
Journal of Orthopaedic Translation,
Journal Year:
2025,
Volume and Issue:
51, P. 242 - 255
Published: March 1, 2025
The
production
of
reactive
oxygen
species
(ROS)
and
mitochondrial
dysfunction
in
chondrocytes
are
closely
related
to
cartilage
degeneration
the
procedure
osteoarthritis
(OA).
Mitophagy
is
responsible
for
scavenging
ROS
dysfunctional
mitochondria
considered
a
key
therapeutic
target
treatment
OA.
Tiopronin,
classic
thiol
antioxidant,
has
been
widely
studied
various
oxidative
stress-related
diseases.
expression
mitophagy
(PINK1,
PARKIN,
TOMM20)
intact
damaged
OA
patients
was
analyzed
by
Western
blot
histological
analysis.
RNA
sequencing
(RNA-seq)
analysis
performed
explore
molecular
mechanism
tiopronin
regulating
chondrocytes,
then
find
specific
tiopronin.
effects
were
evaluated
model
induced
destabilisation
medial
meniscus
(DMM),
degenerative
with
primary
from
mouse
human
explants
experiment.
downstream
mechanisms
further
investigated
si-RNA
knockdown
mitophagy-related
proteins.
level
negatively
correlated
severity
We
revealed
that
promoted
anabolism
extracellular
matrix
(ECM)
hyaline
alleviates
vitro
vivo
strengthening
mitophagy.
Moreover,
strongly
activated
Bnip3,
protein
anchored
membrane,
subsequently
enhanced
Pink1/Parkin
signaling
pathway.
These
findings
indicate
Bnip3-Pink1-Parkin
pathway,
targeted
tiopronin,
plays
role
inhibiting
progression
As
classical
drug
clinic,
developed
new
approach
via
this
study.
Based
significant
efficient
effect
degermation
delay
OA,
it
believed
may
become
an
effective
candidate
clinical
settings.
Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: June 4, 2024
Osteoarthritis
(OA)
is
the
most
common
form
of
arthritis,
characterized
by
osteophyte
formation,
cartilage
degradation,
and
structural
cellular
alterations
synovial
membrane.
Activated
fibroblast-like
synoviocytes
(FLS)
membrane
have
been
identified
as
key
drivers,
secreting
humoral
mediators
that
maintain
inflammatory
processes,
proteases
cause
bone
destruction,
factors
drive
fibrotic
processes.
In
normal
tissue
repair,
processes
are
terminated
after
damage
has
repaired.
fibrosis,
remodeling
wound
healing
exaggerated
prolonged.
Various
stressors,
including
aging,
joint
instability,
inflammation,
lead
to
micro
lesions
within
tissue.
One
result
reduced
production
fluid
(lubricants),
which
reduces
lubricity
areas,
leading
damage.
tissue,
a
wound-healing
cascade
initiated
activating
macrophages,
Th2
cells,
FLS.
The
latter
can
be
divided
into
two
major
populations.
destructive
thymocyte
differentiation
antigen
(THY)1
─
phenotype
restricted
lining
layer.
contrast,
THY1
+
sublining
layer
classified
an
invasive
one
with
immune
effector
function
driving
synovitis.
exact
mechanisms
involved
in
transition
fibroblasts
myofibroblast-like
drives
fibrosis
remain
unclear.
review
provides
overview
phenotypes
spatial
distribution
FLS
OA,
describes
fibroblast
myofibroblast
activation,
metabolic
cells.
Journal of Translational Medicine,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Nov. 11, 2024
Osteoarthritis
(OA)
is
a
chronic
degenerative
joint
condition
characterised
by
cartilage
deterioration
and
changes
in
bone
morphology,
resulting
pain
impaired
mobility.
Investigation
into
the
pathophysiological
mechanisms
underlying
OA
has
highlighted
significance
of
mitochondrial
dysfunction
its
progression.
Mitochondria,
which
are
cellular
organelles,
play
crucial
role
regulating
energy
metabolism,
generating
reactive
oxygen
species,
facilitating
essential
biological
processes
including
apoptosis.
In
recent
years,
utilisation
exogenous
drugs
MT
to
improve
function
chondrocytes
shown
great
promise
treatment.
Numerous
studies
have
investigated
potential
stem
cells
extracellular
vesicles
transfer.
This
review
aims
explore
assess
progress
utilising
transfer
as
therapeutic
approach
for
this
disease.
Journal of Translational Medicine,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Oct. 15, 2024
The
prevalence
of
age-related
degenerative
joint
diseases,
particularly
intervertebral
disc
degeneration
and
osteoarthritis,
is
increasing,
thereby
posing
significant
challenges
for
the
elderly
population.
Mitochondrial
dysfunction
a
critical
factor
in
etiology
progression
these
disorders.
Therapeutic
interventions
that
incorporate
mitochondrial
transplantation
exhibit
considerable
promise
by
increasing
numbers
improving
their
functionality.
Existing
evidence
suggests
exogenous
therapy
improves
clinical
outcomes
patients
with
diseases.
This
review
elucidates
abnormalities
associated
diseases
examines
mechanisms
intercellular
transfer
artificial
transplantation.
Furthermore,
therapeutic
strategies
are
synthesized,
concept
engineered
proposed.
Cells,
Journal Year:
2025,
Volume and Issue:
14(3), P. 151 - 151
Published: Jan. 21, 2025
Osteoarthritis
(OA)
is
the
most
common
rheumatologic
disease
and
a
major
cause
of
pain
disability
in
older
adults.
No
efficient
treatment
currently
available.
Mitochondrial
dysfunction
chondrocytes
drives
molecular
dysregulation
OA
pathogenesis.
Recently,
mitochondrial
transfer
to
had
been
described,
enabling
transplant
mitochondria
as
new
avenue
modify
process,
although
evidence
on
its
feasibility
safety
remains
limited.The
primary
objective
this
study
was
demonstrate
intra-articular
transplantation.
Mitochondria
were
isolated
from
liver
using
procedure
described
by
Preble
coworkers
combined
with
magnetic
beads
coupled
anti-TOM22
antibodies.
The
organelles
obtained
analyzed
determine
their
purity
viability.
viability
administration
into
articular
tissues
well
integration
distribution
transplanted
within
joint
both
vitro
vivo
models.
We
established
an
efficient,
reproducible,
effective,
rapid
protocol
for
isolating
liver.
high
viability,
yield,
purity.
injected
tissue
Functional
detected
extracellular
matrix
cartilage,
menisci
synovium.
Our
results
establish
safe
viable
isolation
injection.
methodology
findings
presented
here
pave
way
future
studies
osteoarthritis
models
validate
transplantation
potentially
effective
OA.
Metabolites,
Journal Year:
2025,
Volume and Issue:
15(2), P. 112 - 112
Published: Feb. 10, 2025
Osteoarthritis
(OA)
and
rheumatoid
arthritis
(RA)
are
joint
diseases
that
share
similar
clinical
features
but
have
different
etiologies,
making
a
differential
diagnosis
particularly
challenging.
Background/Objectives:
Utilizing
advanced
machine
learning
(ML)
techniques
on
metabolomic
data,
this
study
aimed
to
identify
key
metabolites
in
synovial
fluid
(SF)
could
aid
distinguishing
between
OA
RA.
Methods:
Metabolite
data
from
the
MetaboLights
database
(MTBLS564),
analyzed
using
nuclear
magnetic
resonance
(NMR),
were
processed
normalization,
principal
component
analysis
(PCA),
partial
least
squares
discriminant
(PLS-DA)
reveal
prominent
clustering.
Results:
Decision
forests
random
forest
classifiers,
optimized
genetic
algorithms
(GAs),
highlighted
selection
of
few
metabolites—primarily
glutamine,
pyruvate,
proline—with
significant
discriminative
power.
A
Shapley
additive
explanations
(SHAP)
confirmed
these
be
pivotal
predictors,
offering
streamlined
approach
for
diagnostics.
Conclusions:
Our
findings
suggest
minimal
set
can
effectively
relied
upon
distinguish
RA,
supported
by
an
ML
model
achieving
high
accuracy.
This
workflow
streamline
diagnostic
efficiency
enhance
decision-making
rheumatology.
Journal of Inflammation Research,
Journal Year:
2025,
Volume and Issue:
Volume 18, P. 1951 - 1967
Published: Feb. 1, 2025
Abstract:
Osteoarthritis
(OA)
is
a
common
degenerative
joint
disease
characterized
by
the
progressive
degradation
of
articular
cartilage,
synovial
inflammation,
and
subchondral
bone
remodeling.
This
review
explores
interplay
between
aging,
PANoptosis,
inflammation
in
OA
progression.
Age-related
cellular
immune
dysfunctions,
including
senescence,
senescence-associated
secretory
phenotypes
(SASPs),
immunosenescence,
significantly
contribute
to
degeneration.
In
OA,
dysregulated
apoptosis,
necroptosis,
pyroptosis,
particularly
chondrocytes,
exacerbate
cartilage
damage.
Apoptosis,
mediated
JNK
pathway,
reduces
chondrocyte
density,
while
necroptosis
involving
RIPK-1/RIPK-3
NLRP3
inflammasome,
respectively,
amplify
destruction.
Inflammatory
cytokines
damage-associated
molecular
patterns
(DAMPs)
further
enhance
these
PANoptotic
pathways.
Current
therapeutic
strategies
primarily
focus
on
anti-inflammatory
agents
such
as
non-steroidal
drugs
(NSAIDs)
corticosteroids,
with
growing
interest
anti-senescence
targeting
senescence
SASP.
Additionally,
exploring
PANoptosis
mechanisms
offers
potential
for
innovative
treatments.
Keywords:
osteoarthritis,