Microbiome-Driven Therapeutics: From Gut Health to Precision Medicine

Gastrointestinal Disorders, Journal Year: 2025, Volume and Issue: 7(1), P. 7 - 7

Published: Jan. 15, 2025

The human microbiome, a complex ecosystem of microorganisms residing in and on the body, plays pivotal role regulation wide range physiological processes, including digestion, immune responses, metabolic functions. In recent years, rapidly growing field microbiome-driven therapeutics has garnered significant attention owing to its potential revolutionize healthcare. This review explores evolving landscape microbiome-based therapies, with particular focus gut microbiome implications for both health precision medicine. We highlight advances understanding how microbial communities influence disease pathogenesis treatment outcomes, spanning conditions such as inflammatory bowel (IBD), disorders, neurological diseases, even cancer. article also discusses emerging therapeutic strategies, probiotics, prebiotics, fecal microbiota transplantation (FMT), microbial-based drugs, well challenges associated their clinical implementation. Additionally, we examined integration profiling metagenomic data is advancing medicine, paving way personalized effective treatments. serves comprehensive resource that synthesizes current knowledge, identifies key gaps research, offers insights into future direction therapeutics, thus providing valuable framework clinicians, researchers, policymakers seeking harness microbiomes advance healthcare solutions.

Language: Английский

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The frontier of health: Exploring therapeutic potentials of the microbiome

PharmaNutrition, Journal Year: 2025, Volume and Issue: unknown, P. 100435 - 100435

Published: Feb. 1, 2025

Language: Английский

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Exploring effects of gut microbiota on tertiary lymphoid structure formation for tumor immunotherapy

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: March 7, 2025

Anti-tumor immunity, including innate and adaptive immunity is critical in inhibiting tumorigenesis development of tumor. The needs specific lymph organs such as tertiary lymphoid structures (TLSs), which are highly correlated with improved survival outcomes many cancers. In recent years, increasing attention on the TLS tumor microenvironment, TLSs have emerged a novel target for anti-tumor therapy. Excitingly, studies shown contribution to immune responses. However, it unclear how form more effectively defense against through formation. Recent that inflammation plays role Interestingly, also found gut microbiota can regulate occurrence inflammation. Therefore, we here summarize potential effects microbiota- mediated or immunosuppression formation environments. Meanwhile, this review explores manipulate mature regulating microbiota/metabolites associated signal pathways potentially lead next-generation cancer immunotherapy.

Language: Английский

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Exploring the Probiotic Potential of Bacteroides spp. Within One Health Paradigm

Probiotics and Antimicrobial Proteins, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Abstract Probiotics are pivotal in maintaining or restoring the balance of human intestinal microbiota, a crucial factor mitigating diseases and preserving host’s health. Exploration into Bacteroides spp. reveals substantial promise their development as next-generation probiotics due to profound interaction with host immune cells capability regulate microbiome’s metabolism by significantly impacting metabolite production. These beneficial bacteria exhibit potential ameliorating various health issues such disorders, cardiovascular diseases, behavioral even cancer. Though it’s important note that high percentage them well opportunistic pathogens, posing risks under certain conditions. Studies highlight role modifying responses improving conditions regulating lymphocytes, controlling metabolism, preventing inflammation The safety efficacy strains currently scrutiny European Commission for authorization food processing, marking significant step towards commercialization. recent advancements bacterial isolation sequencing methodologies, coupled integration Metagenome-Assembled Genomes (MAGs) binning from metagenomics data, continue unveil spp., aiding broader understanding application these novel disease management.

Language: Английский

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Navigating commensal dysbiosis: Gastrointestinal host-pathogen interplay orchestrating opportunistic infections

Microbiological Research, Journal Year: 2024, Volume and Issue: 286, P. 127832 - 127832

Published: July 3, 2024

Language: Английский

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The Most Promising Next-Generation Probiotic Candidates—Impact on Human Health and Potential Application in Food Technology

Fermentation, Journal Year: 2024, Volume and Issue: 10(9), P. 444 - 444

Published: Aug. 23, 2024

A substantial body of research indicates that the gut microbiota exerts a profound influence on host health. The purpose this work was to characterize selected, most promising, well-known next-generation probiotics (NGPs) and review potential applications bacteria in food technology. isolation with significant health benefits has led emergence NGPs. In contrast traditional probiotics, these originate directly from microbiota, thereby ensuring their optimal adaptation intestinal ecosystem. NGPs exert effects organism through variety mechanisms, including synthesis bioactive compounds, modulation metabolism substances provided by host. Several bacterial species have been identified as candidates for NGPs, Akkermansia muciniphila, Faecalibacterium prausnitzii, Bacteroides thetaiotaomicron, Christensenella minuta, many others. These demonstrated capacity beneficial effects, reduction obesity, type 2 diabetes, metabolic disorders, even cancers. greatest limitation commercialization is lack oxygen tolerance, which presents challenges not only but also application food. approach appears be microencapsulation. Further required establish safety NGP supplementation protect them environmental conditions.

Language: Английский

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Modulatory effects of traditional Chinese medicines on gut microbiota and the microbiota-gut-x axis

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 9, 2024

The gut microbiota offers numerous benefits to the human body, including promotion of nutrient absorption, participation in metabolic processes, and enhancement immune function. Recent studies have introduced concept gut-organ axis, which encompasses interactions such as gut-brain gut-liver gut-lung axis. This underscores complex interplay between various organs tissues, brain, heart, lungs, liver, kidneys, muscles, bones. Growing evidence indicates that can influence onset progression multi-organ system diseases through their effects on Traditional Chinese medicine has demonstrated significant efficacy regulating gastrointestinal system, leveraging its unique advantages. Considerable advancements been made understanding role axis within mechanisms action traditional medicine. review aims elucidate roles health, explore potential connections microbiota, examine therapeutic microbiota-gut-organ Furthermore, addresses limitations challenges present current research while proposing directions for future investigations this area.

Language: Английский

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Molecular mimicry in the pathogenesis of autoimmune rheumatic diseases

Journal of Translational Autoimmunity, Journal Year: 2025, Volume and Issue: 10, P. 100269 - 100269

Published: Jan. 8, 2025

Autoimmune rheumatic diseases (ARDs) are a heterogeneous group of conditions characterized by excessive and misdirected immune responses against the body's own musculoskeletal tissues. Their exact aetiology remains unclear, with genetic, demographic, behavioural environmental factors implicated in disease onset. One prominent hypothesis for initial breach tolerance (leading to autoimmunity) is molecular mimicry, which describes structural or sequence similarities between human microbial proteins (mimotopes). This similarity can lead cross-reactive antibodies T-cell receptors, resulting an response autoantigens. Both commensal microbes microbiome pathogens trigger thereby potentially contributing onset ARDs. In this review, we focus on role mimicry rheumatoid arthritis systemic lupus erythematosus. Moreover, implications also briefly discussed ankylosing spondylitis, sclerosis myositis.

Language: Английский

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Exploring new Bacteroidota strains: Functional Diversity and Probiotic Characteristics

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 4, 2025

Abstract Bacteroidota, a diverse phylum of bacteria, are increasingly recognized for their significant contributions to host health, particularly through antimicrobial and probiotic properties. This study investigates the functional diversity potential 42 new Bacteroidota strains enriched identified from hosts, including mouse ceca human stool samples. Using 16S rRNA gene sequencing, we phylogenetically characterized genera Bacteroides, Phocaeicola Sphingobacterium assessed properties related potential. The were evaluated concerning ability inhibit biofilm formation WHO declared clinically pathogens, gram-positive Staphylococcus aureus epidermidis , gram-negative Klebsiella oxytoca Pseudomonas aeruginosa eukaryotic fungus Candida albicans. Additionally, investigated bile salt hydrolase quorum quenching activities strains, key traits associated with efficacy. Our findings demonstrate that all examined consistently exhibit capacity but different extent. Furthermore, 14 showed activity, 39 highlighting High inhibition as well activity against both autoinducers, AHL AI-2, predominantly observed in Bacteroides caecimuris muris making them attractive candidates next-generation probiotics. Overall, this advances field probiotics by identifying promising therapeutic applications potentially revolutionizing approaches microbiome-based interventions pathogen control clinical settings.

Language: Английский

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A Simplified Method for the Isolation of Extracellular Vesicles from Probiotic Bacteria and Their Characterization

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1058 - 1058

Published: Jan. 26, 2025

Probiotic bacteria are normal inhabitants of a healthy human gut, conferring multiple beneficial effects on the gut and beyond. Under various disease states, abundance diversity significantly decreased, process called dysbiosis. Among intra- extracellular components probiotics, vesicles (EVs) secreted by them have recently garnered significant attention as potential mediators probiotics’ host health. Further, these nanosized particles that encapsulate wide range bioactive molecules (proteins, lipids, RNA, DNA) standing out key factors could mediate microbiota–host communication confer ameliorating in experimental inflammatory, metabolic, cardiovascular models. However, standard protocol EV isolation from probiotic bacteria, not varying lab to lab, must be established achieve consistency results pre-clinical Our current study compared two commonly used methods for biological samples, ultracentrifugation precipitation, develop isolating EVs probiotics Lactobacillus acidophilus (LA), Gram-positive bacterium, Escherichia coli Nissle (EcN), Gram-negative bacterium. The method gave ~1.5-fold higher yield both LA EcN precipitation method. released level LA. were quantified characterized nanoparticle-tracking analysis (NTA) measuring specific surface biomarkers using Western blot. Here, we describe our standardized step-by-step their characterization.

Language: Английский

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