Serum levels of neurotensin, pannexin-1, and sestrin-2 and the correlations with sleep quality or/and cognitive function in the patients with chronic insomnia disorder DOI Creative Commons
Ai-Xi Su,

Zi-Jie Ma,

Zong-Yin Li

et al.

Frontiers in Psychiatry, Journal Year: 2024, Volume and Issue: 15

Published: May 13, 2024

To examine serum concentrations of neurotensin, pannexin-1 and sestrin-2, their correlations with subjective objective sleep quality cognitive function in the patients chronic insomnia disorder (CID). Sixty-five CID were enrolled continuously fifty-six good sleepers same period served as healthy controls (HCs). Serum levels sestrin-2 measured by enzyme-linked immunosorbent assays. Sleep was assessed Pittsburgh Quality Index (PSQI) polysomnography, mood evaluated 17-item Hamilton Depression Rating Scale. General Chinese-Beijing Version Montreal Cognitive Assessment spatial memory Blue Velvet Arena Test (BVAT). Relative to HCs, sufferers had higher neurotensin (t=5.210, p<0.001) (Z=-4.169, p<0.001), lower level (Z=-2.438, p=0.015). In terms measures, positively associated total time (r=0.562, p=0.002) efficiency (r=0.588, p=0.001), negatively wake after onset (r=-0.590, p=0.001) p=0.001); percentage rapid eye movement (r=0.442, p=0.016) non-rapid stage 2 (r=-0.394, p=0.034). Adjusted for sex, age HAMD, still above but only (r=-0.446, p=0.022). However, these biomarkers showed no significant (PSQI score). mean erroneous distance BVAT. depression, MoCA score (r=-0.257, p=0.044), BVAT (r=0.270, p=0.033). The increased decreased levels, indicating neuron dysfunction, which could be related poor dysfunction objectively.

Language: Английский

Hyperexcitation of the glutamatergic neurons in lateral hypothalamus induced by chronic pain contributes to depression-like behavior and learning and memory impairment in male mice DOI Creative Commons

Lianghui Meng,

Xuefeng Zheng,

Keman Xie

et al.

Neurobiology of Stress, Journal Year: 2024, Volume and Issue: 31, P. 100654 - 100654

Published: June 1, 2024

Chronic pain can induce mood disorders and cognitive dysfunction, such as anxiety, depression, learning memory impairment in humans. However, the specific neural network involved anxiety- depression-like behaviors caused by chronic remains poorly understood. In this study, behavioral test results showed that induced behaviors, male mice. c-Fos immunofluorescence fiber photometry recording glutamatergic neurons LH of mice with were selectively activated. Next, normal activated using optogenetic chemogenetic methods, which recapitulates some depressive-like well impairment, but not anxiety-like behavior. Finally, inhibition pain, effectively relieved impairment. Taken together, our findings suggest hyperexcitation is behavior pain.

Language: Английский

Citations

4
Evans Blue Acts as a Selective Inhibitor of CaMKII‐α to Impede the Progression of TCL Identified by HTS DOI Creative Commons

Jianru Tian,

Aiqin Zhang,

Lansong Li

et al.

Cancer Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

T-cell lymphoma (TCL) poses a significant challenge in clinical oncology, characterized by its aggressive behavior and resistance to conventional therapies. Despite considerable research efforts, the prognosis for TCL patients remains poor, primarily due lack of effective therapeutic strategies that can inhibit tumor progression metastasis. In this study, we identified CaMKII-α as potential target TCL. To explore role pathogenesis, investigated effects on cell lines. Protein expression levels within PI3K-AKT signaling pathway were assessed using western blot analysis. Through siRNA-mediated gene silencing, downregulated monitored proliferation. Furthermore, Evans Blue (IC50 = 197.1 nM) selective small-molecule inhibitor through high-throughput screening (HTS). demonstrated tumor-suppressive effects, potentially inhibiting proliferation via regulation pathway. Notably, antitumor effect was comparable observed with genetic ablation, highlighting novel strategy treatment

Language: Английский

Citations

0
GHS-R1a signaling drives anxiety-related behavior by shaping excitability of ventromedial hypothalamic neurons DOI Creative Commons
Meng Zhang, Liu Yang, Xue Mi

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 24, 2025

The neural substrates of anxiety are poorly understood, which hinders treatment disorders. Here we found, αCaMKII+ neurons in the ventral medial hypothalamic nucleus (VMH) responded to stressors with increased activity male mice, both under physiological conditions and after repeated restraint stress. Activation VMH were necessary sufficient ameliorate stress-induced anxiety. peripheral metabolic hormone ghrelin receptor GHS-R1a play a complex role emotion regulation; however, mechanism is uncertain. A delayed increase expression coincided development enhancement anxiety-related behavior. promoted behavior, whereas knockdown had opposite effect. upregulation inhibited excitability neurons. We conclude that GHSR1a signaling drives by shaping may be therapeutic target for treating disorders such as post-traumatic stress disorder.

Language: Английский

Citations

0
The altered hypothalamic network functional connectivity in chronic insomnia disorder and regulation effect of acupuncture: a randomized controlled neuroimaging study DOI Creative Commons
Wei Peng, Hao Xu,

Chuanzhi Zhang

et al.

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 14, 2024

The hypothalamus has been recognized as a core structure in the sleep-wake cycle. However, whether neuroplasticity of is involved acupuncture treatment insomnia remains elusive. We recruited 42 patients with chronic disorder (CID) and 23 matched healthy controls (HCs), CID randomly assigned to receive real (RA) or sham (SA) for four weeks. Insomnia severity was evaluated using Pittsburgh Sleep Quality Index (PSQI) score, resting-state functional connectivity (rsFC) assessed via magnetic resonance imaging (fMRI). In cross-sectional investigation, showed increased rsFC between medial (MH) left lateral orbital frontal cortex (LOFC), bilateral (MOFC) compared HCs. longitudinal experiment, PSQI scores significantly decreased RA group (p = 0.03) but not SA group. Interestingly, MH-LOFC found be reduced following treatment. addition, altered correlated clinical improvement (r -0.692, p 0.006). This randomized neuroimaging study provides preliminary evidence that may improve symptoms by restoring circuits associated hypothalamic subregions. trial registered on Chinese Clinical Trial Registry ( www.chictr.org.cn ) identifier (ChiCTR1800017092). Registered date: 11/07/2018.

Language: Английский

Citations

1
Ventromedial hypothalamus relays chronic stress inputs and exerts bidirectional regulation on anxiety state and related sympathetic activity DOI Creative Commons
Jie Shao, Yan Chen, Dashuang Gao

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2023, Volume and Issue: 17

Published: Dec. 14, 2023

Chronic stress can induce negative emotion states, including anxiety and depression, leading to sympathetic overactivation disturbed physiological homeostasis in peripheral tissues. While anxiety-related neural circuitry integrates chronic information modulates nervous system (SNS) activity, the critical nodes linking activity still need be clarified. In our previous study, we demonstrated that ventromedial hypothalamus (VMH) is involved integrating inputs exerting influence on activity. However, underlying synaptic electrophysiological mechanisms remain elusive. this combined vitro recordings, behavioral tests, optogenetic manipulations, SNS analyses explore role of VMH Results showed played an important bidirectionally regulating anxiety-like behavior excitation. enhanced excitatory into neurons by strengthening connection with paraventricular (PVN), hence promoting tone outflow, factor contributing development metabolic imbalance tissues cardiovascular diseases.

Language: Английский

Citations

3
Serum levels of neurotensin, pannexin-1, and sestrin-2 and the correlations with sleep quality or/and cognitive function in the patients with chronic insomnia disorder DOI Creative Commons
Ai-Xi Su,

Zi-Jie Ma,

Zong-Yin Li

et al.

Frontiers in Psychiatry, Journal Year: 2024, Volume and Issue: 15

Published: May 13, 2024

To examine serum concentrations of neurotensin, pannexin-1 and sestrin-2, their correlations with subjective objective sleep quality cognitive function in the patients chronic insomnia disorder (CID). Sixty-five CID were enrolled continuously fifty-six good sleepers same period served as healthy controls (HCs). Serum levels sestrin-2 measured by enzyme-linked immunosorbent assays. Sleep was assessed Pittsburgh Quality Index (PSQI) polysomnography, mood evaluated 17-item Hamilton Depression Rating Scale. General Chinese-Beijing Version Montreal Cognitive Assessment spatial memory Blue Velvet Arena Test (BVAT). Relative to HCs, sufferers had higher neurotensin (t=5.210, p<0.001) (Z=-4.169, p<0.001), lower level (Z=-2.438, p=0.015). In terms measures, positively associated total time (r=0.562, p=0.002) efficiency (r=0.588, p=0.001), negatively wake after onset (r=-0.590, p=0.001) p=0.001); percentage rapid eye movement (r=0.442, p=0.016) non-rapid stage 2 (r=-0.394, p=0.034). Adjusted for sex, age HAMD, still above but only (r=-0.446, p=0.022). However, these biomarkers showed no significant (PSQI score). mean erroneous distance BVAT. depression, MoCA score (r=-0.257, p=0.044), BVAT (r=0.270, p=0.033). The increased decreased levels, indicating neuron dysfunction, which could be related poor dysfunction objectively.

Language: Английский

Citations

0