Advancements in Plasma Agriculture: A Review of Recent Studies

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(20), P. 15093 - 15093

Published: Oct. 11, 2023

This review is devoted to a topic of high interest in recent times—the use plasma technologies agriculture. The increased attention these studies primarily due the demand for intensification food production and, at same time, request reduce pesticides. We analyzed publications, focusing on research conducted last 3 years, identify main achievements agrotechnologies and key obstacles their widespread implementation practice. considered types sources used this area, advantages limitations, which determine areas application. also plasma-activated liquids efficiency by various sources.

Language: Английский

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Cold atmospheric plasma induces the curing mechanism of diabetic wounds by regulating the oxidative stress mediators iNOS and NO, the pyroptotic mediators NLRP-3, Caspase-1 and IL-1β and the angiogenesis mediators VEGF and Ang-1

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 169, P. 115934 - 115934

Published: Nov. 24, 2023

It has been demonstrated that cold atmospheric plasma (CAP) accelerates the wound healing process, however underlying molecular pathways behind this effect remain unclear. Thus, goal of proposed investigation is to elucidate therapeutic advantages CAP on angiogenesis, pyroptotic, oxidative stress, and inflammatory mediators during wound-healing mechanisms associated with diabetes. Intraperitoneal administration streptozotocin (STZ, 60 mg/Kg) body weight was used induce type-1 Seventy-five male mice were randomized into 3 groups: control non-diabetic group, diabetic group not treated, treated CAP. The key pyroptosis its impact slow process wounds examined using histological investigations employing H&E staining, immunohistochemistry, ELISA, Western blotting analysis. Angiogenesis proteins (VEGF, Ang-1, HO-1) showed a significant decline in expression concentrations wounds, indicating animals' less likely heal. Furthermore, compared controls, major (NLRP-3, IL-1β, caspase-1), stress (iNOS NO), inflammation (TNF-α IL-6) have higher levels wounds. These factors substantially impede mechanism Interestingly, our results disclosed impacts treatment via significantly regulating pyroptosis, pro-inflammatory mediators. Our findings curative likelihood for enhancing

Language: Английский

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A Low Cost, Flexible Atmospheric Pressure Plasma Jet Device With Good Antimicrobial Efficiency

IEEE Transactions on Radiation and Plasma Medical Sciences, Journal Year: 2023, Volume and Issue: 8(3), P. 307 - 322

Published: Dec. 13, 2023

Plasma sources suitable to generate low-temperature plasmas has been fundamental for the advances in plasma medicine. In this research field, must comply with stringent conditions clinical applications. The main requirement be met is patient and operator's safety ethical of effectivity, which encompasses electrical regulations, potential device toxicity, effectiveness relation desired treatment. All these issues are addressed by German prestandard DIN SPEC 91315:2014–06 (DINSpec), deals limits, risk assessment, biological efficacy aimed medical work, a low cost, user-friendly, flexible atmospheric pressure jet (APPJ) was characterized following DINSpec guidelines. device, still under development, proved safe It capable producing an APPJ leakage current ultraviolet emission, gas temperature lower than 40 °C, production harmful gases within limits cytotoxicity. most differentiating feature that presented good antimicrobial even operating at frequency order just few hundred Hz, value below devices reported literature.

Language: Английский

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Bacterial Decontamination of Water-Containing Objects Using Piezoelectric Direct Discharge Plasma and Plasma Jet

Biomolecules, Journal Year: 2024, Volume and Issue: 14(2), P. 181 - 181

Published: Feb. 2, 2024

Cold atmospheric plasma has become a widespread tool in bacterial decontamination, harnessing reactive oxygen and nitrogen species to neutralize bacteria on surfaces the air. This technology is often employed healthcare, food processing, water treatment, etc. One of most energy-efficient universal methods for creating cold initiation piezoelectric direct discharge. The article presents study bactericidal effect discharge generated using multifunctional source "CAPKO". device allows modification method generation "on fly" by replacing unit (cap) working device. results buffer solution modes air jet with an argon flow are presented. these types against E. coli BL21 (DE3) was studied. issues scaling treatment technique considered.

Language: Английский

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Efficacy of non -thermal pressure plasma versus other modalities for disinfection of primary root canals

BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 11, 2025

Language: Английский

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Inactivation of Candida albicans, Staphylococcus aureus and multidrug-resistant Escherichia coli with dielectric barrier discharged cold atmospheric plasma: a comparative study with antimicrobial drugs

Journal of Medical Microbiology, Journal Year: 2025, Volume and Issue: 74(1)

Published: Jan. 29, 2025

Introduction. Cold atmospheric plasma (CAP) has emerged as a promising technology for neutralizing microbes, including multidrug-resistant strains. This study investigates CAP's potential an alternative to traditional antimicrobial drugs microbial inactivation.Hypothesis/Gap Statement. In the era of increasing resistance, there is persistent need strategies. CAP exerts its effects by generating reactive oxygen and nitrogen species (RONS), but comparative efficacy against requires further exploration.Aim. To evaluate in inactivating Escherichia coli (ATCC BAA-2469), Staphylococcus aureus (MTCC 96) Candida albicans 227) compare effectiveness with standard drugs.Methodology. CAP, produced indigenously developed dielectric barrier discharge (DBD) setup comprising quartz-glass-covered high-voltage electrode grounded stainless steel mesh electrode, was used treat three pathogenic samples varying treatment times (0-60 s). The zone inhibition (ZoI; where microbes cannot grow) induced compared ZoI selected (5-300 mcg). Scanning electron microscopy (SEM) analysed morphological changes, while optical emission spectroscopy (OES) detected RONS generated during treatment. Growth curve analysis assessed impact on growth, statistical CAP-induced drug-induced ZoI.Results. substantial E. coli, S. C. albicans, largest (1194±35.35 mm²) after 60 s. DBD-CAP showed equivalent or superior based comparisons. SEM revealed extensive cellular damage all pathogens, visible disruption within significant delay proliferation exposure, effectively inhibiting growth over 24 h. OES confirmed presence RONS-related molecular bands [N2(C-B), N2 +(B-X) OH(A-X)] atomic O lines CAP.Conclusion. exhibits activity drugs. disintegrating morphology delaying growth. These findings highlight prolonged treatments, addressing resistance advancing clinical

Language: Английский

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Effect of Flexible Plasma Pad on HaCaT Cells and Bacteria

Plasma Processes and Polymers, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

ABSTRACT Dielectric barrier discharge (DBD) is a method that promotes wound healing. This study designed flexible plasma pad with polyimide film as the dielectric layer and patterned copper foil ground electrode. The was used to treat normal skin cells bacteria at site. Cell viability, intracellular extracellular active substances, Western blot analysis indicated LTP can activate PI3K/AKT/mTOR signaling pathway, decrease E‐Cadherin expression, promote HaCaT cell proliferation. It exhibits antibacterial activity against Staphylococcus aureus Escherichia coli . results of this suggest an efficient device for treating wounds bacterial infections.

Language: Английский

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Helium cold atmospheric pressure plasma reduces erastin induced inflammation and ferroptosis in human gingival fibroblasts

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 3, 2025

Oral soft tissue damage can lead to hard in the oral cavity, such as periodontal lesions, periapical disorders, cysts, and tumors. Cold plasma is known alleviate inflammation oxidative stress promote regeneration, yet effects of helium on human gingival cells remain poorly understood. In this study, we examined whether (He) cold atmospheric pressure (CAP) induce anti-inflammatory anti-ferroptotic tissues by ionizing He gas. Erastin treatment followed CAP exposure fibroblast-1 (HGF-1) reduced mRNA expression inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), tumor necrosis factor-α (TNFα), interleukin-6 (IL-6), which are linked inflammatory responses. Additionally, decreased nuclear receptor coactivator 4 (NCOA4) increased glutathione peroxidase (GPX4) expression. Furthermore, mitochondrial membrane potential was restored voltage-dependent anion channel 1 (VDAC1) expression, reactive oxygen species (ROS) levels mitochondria cytoplasm were reduced. These results suggest that may be associated with modulation ROS production reduction ferroptosis, but repair contributes these requires further investigation.

Language: Английский

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Innovative Therapeutic Approach Targeting Colon Cancer Stem Cells: Transitional Cold Atmospheric Plasma

ACS Omega, Journal Year: 2025, Volume and Issue: 10(12), P. 12109 - 12121

Published: March 6, 2025

Transitional cold atmospheric plasma (TCAP) represents a novel technique for generating remotely from primary source. It consists of partially nonthermal ionized gas mixture containing charged and neutral particles, photons, free radicals. In recent years, TCAP has attracted considerable attention in biomedical applications. order to evaluate colon cancer stem cells' (CCSCs) proliferation, apoptotic induction, inflammatory response, survival, was utilized both directly indirectly this study. Using argon helium gases, continuously delivered two stages during the experiment. For direct state, irradiated onto CCSCs 3 5 min. indirect technique, Matrigel treated with min before introduction cells. vitro assays demonstrated that exposure significantly reduced viability CCSCs; application had greater impacts than argon. Numerous investigations confirmed induction apoptosis, showing groups more cells altered cellular structures controls (****p < 0.0001). A substantial increase Bax/Bcl-2 ratio found by analyzing expression Bax Bcl-2 genes, indicating increased susceptibility apoptosis (*p = 0.0177 ***p 0.0004). The higher efficacy mode further highlighted marker analysis, which showed significant reduction interleukin-6 interleukin-8 TCAP-helium compared TCAP-argon (**p 0.0015 0.0007). Lastly, proliferation test, relies on Ki-67 expression, noteworthy decline all TCAP-treated groups, group exhibiting most robust impact 0.0014). Overall, findings highlight potential TCAP, particularly helium, as promising approach selectively targeting providing insights into its therapeutic mechanisms treatment. therefore, emerges unique strategy applications cell-targeted therapies.

Language: Английский

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Effect of plasma-activated water on body weight, behavior, biochemical profile, intestinal microbiota, and liver and kidney histopathology in mice

Deleted Journal, Journal Year: 2025, Volume and Issue: 7(5)

Published: April 17, 2025

Abstract Plasma-activated water (PAW) is a new technology applied in biomedical research, agriculture, and the food industry. However, its safety long-term effects require further investigation. Therefore, current study was designed to evaluate impact of PAW oral gavage on body weight, intake, behavior (tail suspension test open field test), internal organs relative blood biochemical indicators, intestinal microbiota liver kidney histopathology 10-week-old female mice (n = 30). Mice were divided into three groups each containing 10 received orally (gastric lavage) either distilled (DW), or DW exposed cold atmospheric plasma (CAP) for 3 min (PAW-3), 15 (PAW-15) dose 200 μl/mice (3 times/week) 4 weeks. Compared with DW, results showed that both PAW-3 PAW-15 significantly increased urea concentration ( P 0.044). The group higher neutrophil/lymphocyte ratio 0.043), lactobacilli count 0.002). alkaline phosphatase levels 0.039), lower coliform < 0.001). No significant differences > 0.05) organ weights, tests, major parameters observed. Liver tissues PAW-treated groups, particularly PAW-15, exhibited severe pathological changes, including hepatocellular necrosis, glomerular atrophy, interstitial inflammation, tubular degeneration, indicating substantial damage associated exposure. In conclusion, PAW's markers, microbiota, health raises concerns, warranting studies.

Language: Английский

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