Cold
atmospheric
plasma
(CAP)
has
emerged
as
a
promising
therapeutic
technology,
distinguished
by
its
safety,
efficiency,
and
minimal
side
effects.
With
increasing
applications
in
dermatology,
CAP
demonstrates
antimicrobial
efficacy
against
bacteria,
viruses,
fungi,
supports
tissue
proliferation
wound
healing,
hinders
the
growth
migration
of
tumor
cells.
This
review
encompasses
concept,
physical
characteristics,
current
research
dermatology.
CAP,
gas
cloud
produced
ionizing
gases,
operates
at
room
temperature,
ensuring
safety
dermatological
use.
Its
effects
are
mediated
through
active
components
like
reactive
oxygen
nitrogen
species
(RONS)
ultraviolet
radiation.
The
extensive
covered
this
paper
explores
CAP's
skin
infections,
immune-related
diseases,
diseases.
As
biologically
suitable
cold
pressure
finds
widespread
use
medicine,
including
medical
device
sterilization,
dentistry,
oncology,
direct
indirect
forms
applications,
plasma-activated
liquids,
complement
each
other.
holds
promise
treatment
technique,
but
further
exploration
needed.
article
briefly
diverse
potential
utility
within
objective
is
to
enhance
progress
medicine
furnishing
concise
overview
groundbreaking
technology
contexts.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(20), P. 15093 - 15093
Published: Oct. 11, 2023
This
review
is
devoted
to
a
topic
of
high
interest
in
recent
times—the
use
plasma
technologies
agriculture.
The
increased
attention
these
studies
primarily
due
the
demand
for
intensification
food
production
and,
at
same
time,
request
reduce
pesticides.
We
analyzed
publications,
focusing
on
research
conducted
last
3
years,
identify
main
achievements
agrotechnologies
and
key
obstacles
their
widespread
implementation
practice.
considered
types
sources
used
this
area,
advantages
limitations,
which
determine
areas
application.
also
plasma-activated
liquids
efficiency
by
various
sources.
Plasma Processes and Polymers,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 4, 2025
ABSTRACT
Dielectric
barrier
discharge
(DBD)
is
a
method
that
promotes
wound
healing.
This
study
designed
flexible
plasma
pad
with
polyimide
film
as
the
dielectric
layer
and
patterned
copper
foil
ground
electrode.
The
was
used
to
treat
normal
skin
cells
bacteria
at
site.
Cell
viability,
intracellular
extracellular
active
substances,
Western
blot
analysis
indicated
LTP
can
activate
PI3K/AKT/mTOR
signaling
pathway,
decrease
E‐Cadherin
expression,
promote
HaCaT
cell
proliferation.
It
exhibits
antibacterial
activity
against
Staphylococcus
aureus
Escherichia
coli
.
results
of
this
suggest
an
efficient
device
for
treating
wounds
bacterial
infections.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: March 3, 2025
Oral
soft
tissue
damage
can
lead
to
hard
in
the
oral
cavity,
such
as
periodontal
lesions,
periapical
disorders,
cysts,
and
tumors.
Cold
plasma
is
known
alleviate
inflammation
oxidative
stress
promote
regeneration,
yet
effects
of
helium
on
human
gingival
cells
remain
poorly
understood.
In
this
study,
we
examined
whether
(He)
cold
atmospheric
pressure
(CAP)
induce
anti-inflammatory
anti-ferroptotic
tissues
by
ionizing
He
gas.
Erastin
treatment
followed
CAP
exposure
fibroblast-1
(HGF-1)
reduced
mRNA
expression
inducible
nitric
oxide
synthase
(iNOS),
cyclooxygenase-2
(COX-2),
interleukin-1β
(IL-1β),
tumor
necrosis
factor-α
(TNFα),
interleukin-6
(IL-6),
which
are
linked
inflammatory
responses.
Additionally,
decreased
nuclear
receptor
coactivator
4
(NCOA4)
increased
glutathione
peroxidase
(GPX4)
expression.
Furthermore,
mitochondrial
membrane
potential
was
restored
voltage-dependent
anion
channel
1
(VDAC1)
expression,
reactive
oxygen
species
(ROS)
levels
mitochondria
cytoplasm
were
reduced.
These
results
suggest
that
may
be
associated
with
modulation
ROS
production
reduction
ferroptosis,
but
repair
contributes
these
requires
further
investigation.
ACS Omega,
Journal Year:
2025,
Volume and Issue:
10(12), P. 12109 - 12121
Published: March 6, 2025
Transitional
cold
atmospheric
plasma
(TCAP)
represents
a
novel
technique
for
generating
remotely
from
primary
source.
It
consists
of
partially
nonthermal
ionized
gas
mixture
containing
charged
and
neutral
particles,
photons,
free
radicals.
In
recent
years,
TCAP
has
attracted
considerable
attention
in
biomedical
applications.
order
to
evaluate
colon
cancer
stem
cells'
(CCSCs)
proliferation,
apoptotic
induction,
inflammatory
response,
survival,
was
utilized
both
directly
indirectly
this
study.
Using
argon
helium
gases,
continuously
delivered
two
stages
during
the
experiment.
For
direct
state,
irradiated
onto
CCSCs
3
5
min.
indirect
technique,
Matrigel
treated
with
min
before
introduction
cells.
vitro
assays
demonstrated
that
exposure
significantly
reduced
viability
CCSCs;
application
had
greater
impacts
than
argon.
Numerous
investigations
confirmed
induction
apoptosis,
showing
groups
more
cells
altered
cellular
structures
controls
(****p
<
0.0001).
A
substantial
increase
Bax/Bcl-2
ratio
found
by
analyzing
expression
Bax
Bcl-2
genes,
indicating
increased
susceptibility
apoptosis
(*p
=
0.0177
***p
0.0004).
The
higher
efficacy
mode
further
highlighted
marker
analysis,
which
showed
significant
reduction
interleukin-6
interleukin-8
TCAP-helium
compared
TCAP-argon
(**p
0.0015
0.0007).
Lastly,
proliferation
test,
relies
on
Ki-67
expression,
noteworthy
decline
all
TCAP-treated
groups,
group
exhibiting
most
robust
impact
0.0014).
Overall,
findings
highlight
potential
TCAP,
particularly
helium,
as
promising
approach
selectively
targeting
providing
insights
into
its
therapeutic
mechanisms
treatment.
therefore,
emerges
unique
strategy
applications
cell-targeted
therapies.
Biomedicine & Pharmacotherapy,
Journal Year:
2023,
Volume and Issue:
169, P. 115934 - 115934
Published: Nov. 24, 2023
It
has
been
demonstrated
that
cold
atmospheric
plasma
(CAP)
accelerates
the
wound
healing
process,
however
underlying
molecular
pathways
behind
this
effect
remain
unclear.
Thus,
goal
of
proposed
investigation
is
to
elucidate
therapeutic
advantages
CAP
on
angiogenesis,
pyroptotic,
oxidative
stress,
and
inflammatory
mediators
during
wound-healing
mechanisms
associated
with
diabetes.
Intraperitoneal
administration
streptozotocin
(STZ,
60
mg/Kg)
body
weight
was
used
induce
type-1
Seventy-five
male
mice
were
randomized
into
3
groups:
control
non-diabetic
group,
diabetic
group
not
treated,
treated
CAP.
The
key
pyroptosis
its
impact
slow
process
wounds
examined
using
histological
investigations
employing
H&E
staining,
immunohistochemistry,
ELISA,
Western
blotting
analysis.
Angiogenesis
proteins
(VEGF,
Ang-1,
HO-1)
showed
a
significant
decline
in
expression
concentrations
wounds,
indicating
animals'
less
likely
heal.
Furthermore,
compared
controls,
major
(NLRP-3,
IL-1β,
caspase-1),
stress
(iNOS
NO),
inflammation
(TNF-α
IL-6)
have
higher
levels
wounds.
These
factors
substantially
impede
mechanism
Interestingly,
our
results
disclosed
impacts
treatment
via
significantly
regulating
pyroptosis,
pro-inflammatory
mediators.
Our
findings
curative
likelihood
for
enhancing
Biomolecules,
Journal Year:
2024,
Volume and Issue:
14(2), P. 181 - 181
Published: Feb. 2, 2024
Cold
atmospheric
plasma
has
become
a
widespread
tool
in
bacterial
decontamination,
harnessing
reactive
oxygen
and
nitrogen
species
to
neutralize
bacteria
on
surfaces
the
air.
This
technology
is
often
employed
healthcare,
food
processing,
water
treatment,
etc.
One
of
most
energy-efficient
universal
methods
for
creating
cold
initiation
piezoelectric
direct
discharge.
The
article
presents
study
bactericidal
effect
discharge
generated
using
multifunctional
source
"CAPKO".
device
allows
modification
method
generation
"on
fly"
by
replacing
unit
(cap)
working
device.
results
buffer
solution
modes
air
jet
with
an
argon
flow
are
presented.
these
types
against
E.
coli
BL21
(DE3)
was
studied.
issues
scaling
treatment
technique
considered.
IEEE Transactions on Radiation and Plasma Medical Sciences,
Journal Year:
2023,
Volume and Issue:
8(3), P. 307 - 322
Published: Dec. 13, 2023
Plasma
sources
suitable
to
generate
low-temperature
plasmas
has
been
fundamental
for
the
advances
in
plasma
medicine.
In
this
research
field,
must
comply
with
stringent
conditions
clinical
applications.
The
main
requirement
be
met
is
patient
and
operator's
safety
ethical
of
effectivity,
which
encompasses
electrical
regulations,
potential
device
toxicity,
effectiveness
relation
desired
treatment.
All
these
issues
are
addressed
by
German
prestandard
DIN
SPEC
91315:2014–06
(DINSpec),
deals
limits,
risk
assessment,
biological
efficacy
aimed
medical
work,
a
low
cost,
user-friendly,
flexible
atmospheric
pressure
jet
(APPJ)
was
characterized
following
DINSpec
guidelines.
device,
still
under
development,
proved
safe
It
capable
producing
an
APPJ
leakage
current
ultraviolet
emission,
gas
temperature
lower
than
40
°C,
production
harmful
gases
within
limits
cytotoxicity.
most
differentiating
feature
that
presented
good
antimicrobial
even
operating
at
frequency
order
just
few
hundred
Hz,
value
below
devices
reported
literature.
Journal of Medical Microbiology,
Journal Year:
2025,
Volume and Issue:
74(1)
Published: Jan. 29, 2025
Introduction.
Cold
atmospheric
plasma
(CAP)
has
emerged
as
a
promising
technology
for
neutralizing
microbes,
including
multidrug-resistant
strains.
This
study
investigates
CAP's
potential
an
alternative
to
traditional
antimicrobial
drugs
microbial
inactivation.Hypothesis/Gap
Statement.
In
the
era
of
increasing
resistance,
there
is
persistent
need
strategies.
CAP
exerts
its
effects
by
generating
reactive
oxygen
and
nitrogen
species
(RONS),
but
comparative
efficacy
against
requires
further
exploration.Aim.
To
evaluate
in
inactivating
Escherichia
coli
(ATCC
BAA-2469),
Staphylococcus
aureus
(MTCC
96)
Candida
albicans
227)
compare
effectiveness
with
standard
drugs.Methodology.
CAP,
produced
indigenously
developed
dielectric
barrier
discharge
(DBD)
setup
comprising
quartz-glass-covered
high-voltage
electrode
grounded
stainless
steel
mesh
electrode,
was
used
treat
three
pathogenic
samples
varying
treatment
times
(0-60
s).
The
zone
inhibition
(ZoI;
where
microbes
cannot
grow)
induced
compared
ZoI
selected
(5-300
mcg).
Scanning
electron
microscopy
(SEM)
analysed
morphological
changes,
while
optical
emission
spectroscopy
(OES)
detected
RONS
generated
during
treatment.
Growth
curve
analysis
assessed
impact
on
growth,
statistical
CAP-induced
drug-induced
ZoI.Results.
substantial
E.
coli,
S.
C.
albicans,
largest
(1194±35.35
mm²)
after
60
s.
DBD-CAP
showed
equivalent
or
superior
based
comparisons.
SEM
revealed
extensive
cellular
damage
all
pathogens,
visible
disruption
within
significant
delay
proliferation
exposure,
effectively
inhibiting
growth
over
24
h.
OES
confirmed
presence
RONS-related
molecular
bands
[N2(C-B),
N2
+(B-X)
OH(A-X)]
atomic
O
lines
CAP.Conclusion.
exhibits
activity
drugs.
disintegrating
morphology
delaying
growth.
These
findings
highlight
prolonged
treatments,
addressing
resistance
advancing
clinical