A guide to the use of bioassays in exploration of natural resources

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 71, P. 108307 - 108307

Published: Jan. 5, 2024

Bioassays are the main tool to decipher bioactivities from natural resources thus their selection and quality critical for optimal bioprospecting. They used both in early stages of compounds isolation/purification/identification, later evaluate safety efficacy. In this review, we provide a comprehensive overview most common bioassays discovery development new bioactive with focus on marine bioresources. We present list practical considerations selecting appropriate discuss detail typically explore antimicrobial, antibiofilm, cytotoxic, antiviral, antioxidant, anti-ageing potential. The concept control bioassay validation introduced, followed by considerations, which advancing higher stage development. conclude providing an application-oriented view focused pharmaceuticals, food supplements, cosmetics, industrial pipelines where currently known products hold highlight importance gaining reliable results, as these serve starting point application-based further testing, well consideration regulatory authorities.

Language: Английский

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Protease-Resistant, Broad-Spectrum Antimicrobial Peptides with High Antibacterial and Antifungal Activity

Life, Journal Year: 2025, Volume and Issue: 15(2), P. 242 - 242

Published: Feb. 5, 2025

Antimicrobial peptides (AMPs) are a diverse group of small, naturally occurring molecules that orchestrate the innate immune response various organisms, from microorganisms to humans. Characterized by their broad-spectrum activity against bacteria, fungi and viruses, AMPs increasingly recognized for potential as novel therapeutic agents in face rising antibiotic resistance. Here, we present several newly designed AMPs, one which, DTN6, exerts significant organisms with MIC values low 0.5 µg/mL. The D-TN6 peptide influences both bacteria yeasts. Scanning electron microscopy transmission results showed bacterial membrane is affected D-TN6, which resistant proteases effective antibiotic-resistant pathogens hemolytic toxicity. vivo standard S. aureus strains wounds. Thus, potent candidate broad spectrum activity. Overall, promising tool development next-generation antimicrobial could mitigate global health threats posed multidrug-resistant pathogens.

Language: Английский

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Physical and Functional Characterization of PLGA Nanoparticles Containing the Antimicrobial Peptide SAAP-148

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2867 - 2867

Published: Feb. 2, 2023

Synthetic antimicrobial and antibiofilm peptide (SAAP-148) commits significant activities against resistant (AMR) planktonic bacteria biofilms. However, SAAP-148 is limited by its low selectivity index, i.e., ratio between cytotoxicity activity, as well bioavailability at infection sites. We hypothesized that formulation of in PLGA nanoparticles (SAAP-148 NPs) improves the index due to sustained local release peptide. The aim this study was investigate physical functional characteristics NPs compare formulated with solution. displayed favorable physiochemical properties [size = 94.1 ± 23 nm, polydispersity (PDI) 0.08 0.1, surface charge 1.65 0.1 mV, encapsulation efficiency (EE) 86.7 0.3%] for up 21 days PBS 37 °C. antibacterial studies showed drastically increased, 10-fold, regarding AMR

Language: Английский

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Bacterial-Nanocellulose-Based Biointerfaces and Biomimetic Constructs for Blood-Contacting Medical Applications

ACS Materials Au, Journal Year: 2023, Volume and Issue: 3(5), P. 418 - 441

Published: June 27, 2023

Understanding the interaction between biomaterials and blood is critical in design of novel for use biomedical applications. Depending on application, can be designed to promote hemostasis, slow or stop bleeding an internal external wound, prevent thrombosis permanent temporary medical implants. Bacterial nanocellulose (BNC) a natural, biocompatible biopolymer that has recently gained interest its potential blood-contacting applications (e.g., artificial vascular grafts), due high porosity, shapeability, tissue-like properties. To BNC been modified through oxidation functionalization with various peptides, proteins, polysaccharides, minerals interact coagulation cascade. For as graft vascularization, extensively researched, studies investigating different modification techniques enhance endothelialization such functionalizing adhesion peptides extracellular matrix (ECM) proteins well tuning structural properties surface roughness, pore size, fiber size. While inherently exhibits comparable mechanical characteristics endogenous vessels, these enhanced chemical altering fabrication method. In this review, we provide comprehensive overview have implemented suitability testing applied evaluate their performance. Initially, focused hemostatic Subsequently, outline methods used production BNC-based grafts generate vasculature tissue engineered constructs. This sequential organization enables clear concise discussion modifications highlights diverse versatile nature natural biomaterial.

Language: Английский

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Synthesized Bis-Triphenyl Phosphonium-Based Nano Vesicles Have Potent and Selective Antibacterial Effects on Several Clinically Relevant Superbugs

Published: July 11, 2024

The increasing emergence of multidrug resistant (MDR) pathogens, due to antibiotics misuse, translates in obstinate infections, with high morbidity and high-cost hospitalizations. To oppose these MDR superbugs, new antimicrobial options are necessary. Although both quaternary ammonium (QASs) phosphonium salts (QPSs) possess effects, QPSs have been studied at lesser extent. Recently, we successfully reported on the bacteriostatic cytotoxic effects a triphenyl salt against isolates Enterococcus Staphylococcus genus. Here, aiming finding antibacterial devices possibly bactericidal towards broader spectrum clinically relevant bacteria, responsible for severe human synthesized water-soluble, sterically hindered (BPPB). It encompasses two groups linked by C12 alkyl chain, thus embodying characteristics molecules known as bola-amphiphiles. BPPB was characterized ATR-FTIR, NMR UV spectroscopy, FIA-MS (ESI), elemental analysis potentiometric titrations. Optical DLS analyses evidenced tendency self-forming spherical vesicles solution (45 nm DLS), having positive zeta potential (+18 mV). were first time assessed fifty clinical Gram-positive Gram-negative species. Excellent observed all strains tested, involving most worrying species included ESKAPE bacteria. lowest MICs 0.250 µg/mL, while highest ones (32 µg/mL) metallo-β-lactamase-producing bacteria and/or also colistin, carbapenems, cefiderocol, so that intractable current available antibiotics. Moreover, when administered HepG2 hepatic Cos-7 monkey kidney cell lines, showed selectivity indices > 10 isolated superbugs such those E. coli species, being very promising be developed.

Language: Английский

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Unlocking the photocatalytic and antibacterial properties of triple and quadruple doped ZnO nanoparticles (Mg/Cu/N-ZnO and Mg/Cu/N/B-ZnO) prepared by one pot facile solid state synthesis

Advanced Powder Technology, Journal Year: 2024, Volume and Issue: 35(9), P. 104567 - 104567

Published: July 27, 2024

Language: Английский

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Streptomyces sp. Strain PBR11, a Forest-Derived Soil Actinomycetia with Antimicrobial Potential

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)

Published: Jan. 31, 2023

The

Language: Английский

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Antibacterial and degradation properties of dialdehyded and aminohexamethylated nanocelluloses

Carbohydrate Polymers, Journal Year: 2023, Volume and Issue: 311, P. 120603 - 120603

Published: Jan. 21, 2023

Dialdehyde cellulose nanofibrils (CNF) and nanocrystals (CNC) were prepared via periodate oxidation (CNF/CNC-ox) subsequently functionalized with hexamethylenediamine (HMDA) a Schiff-base reaction, resulting in partially crosslinked micro-sized (0.5-10 μm) particles (CNF/CNC-ox-HMDA) an aggregation sedimentation tendency aqueous media, as assessed by Dynamic Light Scattering Scanning Electron Microscopy. The antibacterial efficacy, aquatic vivo (to Daphnia magna) human vitro A594 lung cells) toxicities, degradation profiles composting soil of all forms CNF/CNC to define their safety profile. CNF/CNC-ox-HMDA exhibited higher activity than CNF/CNC-ox against Gram-positive S. aureus Gram-negative E. coli, yielding bacteria reduction >90 % after 24 h exposure at the minimum (≤2 mg/mL), but potentially moderately/aquatic low/human toxic concentrations (≥50 mg/L). presence anionic, un/protonated amino-hydrophobized groups addition unconjugated aldehydes hydrodynamically smaller (<1 CNC-ox-HMDA increased both log 9 ≥4 mg/mL bactericidal activity. While only can be considered biosafe up >80 biodegradable within weeks, this process was inhibited for CNF/CNC-ox-HMDA. This indicated different stability, application disposal use (composting vs. recycling).

Language: Английский

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Extraction, Characterization, and Evaluation of the Cytotoxic Activity of Piperine in Its Isolated form and in Combination with Chemotherapeutics against Gastric Cancer

Molecules, Journal Year: 2023, Volume and Issue: 28(14), P. 5587 - 5587

Published: July 22, 2023

Gastric cancer is one of the most frequent types neoplasms worldwide, usually presenting as aggressive and difficult-to-manage tumors. The search for new structures with anticancer potential encompasses a vast research field in which natural products arise promising alternatives. In this scenario, piperine, an alkaloid Piper species, has received attention due to its biological activity, including attributes. present work proposes three heating-independent, reliable, low-cost, selective methods obtaining piperine from nigrum L. (Black pepper). Electronic (SEM) optical microscopies, X-ray diffraction, nuclear magnetic resonance spectroscopies (13C 1H NMR), (UV–Vis, photoluminescence, FTIR) confirm obtention crystals. MTT assay reveals that samples exhibit good cytotoxic activity against primary metastasis models gastric cell lines Brazilian Amazon. showed cytotoxicity on evaluated models, revealing higher effectiveness cells bearing degree aggressiveness. Moreover, investigated crystals demonstrated ability act enhancer when combined traditional chemotherapeutics (5-FU GEM), allowing drugs achieve same effect employing lower concentrations. These results establish molecule therapy investigations cancer, both isolated form or bioenhancer.

Language: Английский

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Bioprospecting of Natural Compounds for Industrial and Medical Applications

Published: June 25, 2021

Bioprospecting is the investigation of biodiversity to search new resources commercial value. The bioprospecting program includes scientific and economic activities that explore genes, organisms, species in diverse habitats ecosystem while safeguarding conservation, traditional knowledge, growth local communities. natural products obtained from have contributed immensely medicinal agricultural fields recently bioremediation, biomimetic engineering, aquaculture, nanotechnology fields. These include pharmaceuticals, industrial chemicals, metabolic pathways, behaviors, materials. They are used as such product development or may be physical blueprints designs leads. Since involves worldwide activities, ethical legal issues also addressed prevent biopiracy other malpractices. In this chapter, we will discuss about bioprospecting, rational processes, their drawbacks, approaches advance utility agricultural, medical,

Language: Английский

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