Production of Protein Hydrolysates from Cod Backbone Using Selected Enzymes: Evaluation of Antioxidative and Antimicrobial Activities of Hydrolysates

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(3), P. 125 - 125

Published: March 13, 2025

In the fish industry, up to 70% of all end as side-streams such backbones, heads, and viscera. To reduce quantities side-streams, a higher utilization degree is needed. The aim this study was use cod backbone for an enzymatic production bioactive hydrolysates with antioxidative and/or antimicrobial properties. Three different enzymes were applied (Alcalase, Neutrase, Protamex), hydrolyses carried out within enzyme’s optima pH temperature 0.5–6 h. efficiency enzyme treatment evaluated based on protein extraction yield (PEY), hydrolysis (DH), antioxidant activity using two in vitro assays (1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging iron chelation) determined by minimum inhibitory concentration (MIC) disk diffusion assays. Selected showing respect amino acid composition molecular weight. Alcalase-treated samples had highest PEY (3 h, 63.5 ± 4.5%) followed Protamex-treated 6 h; 51.9 5.5% 56.5 4.5%); lowest obtained Neutrase 30.4 1.9% 34.7 3.4%). No clear relationship observed between DH. All activities. For activity, hydrolysate showed IC50 (6 2.1 0.1 mg powder/mL) weight <10 kDa, whereas chelation control (no added but heat-treated) similar or lower weights 200–10 kDa. Amino measured selected suggested that not only also sequence size influence None activity. summary, results can be produced from backbone, which makes it possible utilize side-stream generated industry.

Language: Английский

---

Analytical Strategies for Green Extraction, Characterization, and Bioactive Evaluation of Polyphenols, Tocopherols, Carotenoids, and Fatty Acids in Agri-Food Bio-Residues

Molecules, Journal Year: 2025, Volume and Issue: 30(6), P. 1326 - 1326

Published: March 15, 2025

Recent advancements in analytical strategies have enabled the efficient extraction and characterization of bioactive compounds from agri-food bio-residues, emphasizing green chemistry circular economy principles. This review highlights valorization several bio-residues for high-value-added compounds, particularly polyphenols, tocopherols, carotenoids, fatty acids, as a biorefinery approach. To this end, adoption environmentally friendly technologies is essential to improve performance, reduce energy consumption, minimize costs. study therefore examines emerging methodologies such supercritical fluid extraction, pressurized liquid pulsed electric fields, matrix solid-phase dispersion, highlighting their advantages limitations. Additionally, chemical these explored through spectrophotometric high-resolution chromatographic techniques, crucial accurate identification quantification. complemented by an analysis bioactivity assays evaluating antioxidant, antimicrobial, anticancer, neuroprotective, anti-inflammatory properties, with focus on applications food, pharmaceutical, cosmetic industries. However, control toxic alkaloids, undoubtedly needed. Ultimately, approach not only promotes sustainability but also contributes development eco-friendly solutions various

Language: Английский

---

Total Synthesis of Mycinolide IV and Path‐Scouting for Aldgamycin N

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(14), P. 7893 - 7899

Published: Jan. 15, 2021

Abstract Proof‐of‐concept is provided that a large estate of 16‐membered macrolide antibiotics can be reached by “unified” approach. The key building block was formed on scale an asymmetric vinylogous Mukaiyama aldol reaction; its alkene terminus then converted either into the corresponding methyl ketone Wacker oxidation or chain‐extended aldehyde catalyst‐controlled branch‐selective hydroformylation. These transformations ultimately opened access to two structurally distinct series targets. Notable late‐stage maneuvers comprise rare example ruthenium‐catalyzed redox isomerization 1,3‐enyne‐5‐ol 1,3‐diene‐5‐one derivative, as well elaboration tertiary propargylic alcohol acyloin trans ‐hydrostannation/Chan‐Lam‐type coupling. Moreover, this case study illustrates underutilized possibility forging complex macrolactone rings transesterification under essentially neutral conditions.

Language: Английский

---

Overview on Strategies and Assays for Antibiotic Discovery

Pharmaceuticals, Journal Year: 2022, Volume and Issue: 15(10), P. 1302 - 1302

Published: Oct. 21, 2022

The increase in antibiotic resistance poses a major threat to global health. Actinomycetes, the Gram-positive bacteria of order Actinomycetales, are fertile producers bioactive secondary metabolites, including antibiotics. Nearly two-thirds antibiotics that used for treatment bacterial infections were originally isolated from actinomycetes strains belonging genus Streptomyces. This emphasizes importance discovery. However, identification new antimicrobial compound and exploration its mode action very challenging tasks. Therefore, different approaches enable “detection” an characterization mechanisms leading biological activity indispensable. Beyond bioinformatics tools facilitating biosynthetic gene clusters (BGCs), whole cell-screenings—in which cells exposed actinomycete-derived compounds—are common strategy applied at early stage drug development. More recently, target-based have been established. In this case, candidates tested interactions with usually validated targets. review focuses on bioactivity-based screening methods provides readers overview most relevant assays investigation action. Moreover, article includes examples successful application these suggestions improvement.

Language: Английский

---

Antimicrobial-loaded biodegradable nanoemulsions for efficient clearance of intracellular pathogens in bacterial peritonitis

Biomaterials, Journal Year: 2023, Volume and Issue: 302, P. 122344 - 122344

Published: Oct. 10, 2023

Language: Английский

---