Molecules,
Journal Year:
2023,
Volume and Issue:
28(23), P. 7763 - 7763
Published: Nov. 24, 2023
Melanoma
is
one
of
the
most
dangerous
forms
skin
cancer,
characterized
by
early
metastasis
and
rapid
development.
In
search
for
effective
treatment
options,
much
attention
given
to
triterpenoids
plant
origin,
which
are
considered
promising
drug
candidates
due
their
well
described
anticancer
properties
relatively
low
toxicity.
This
paper
comprehensively
summarizes
antimelanoma
potential
natural
triterpenoids,
that
also
used
as
scaffolds
development
more
derivatives.
These
include
betulin,
betulinic
acid,
ursolic
maslinic
oleanolic
celastrol
lupeol.
Some
lesser-known
deserve
in
this
context
22β-hydroxytingenone,
cucurbitacins,
geoditin
A
ganoderic
acids.
Recently
mechanisms
action
presented,
together
with
results
preclinical
vitro
vivo
studies,
use
delivery
systems
pharmaceutical
technologies
improve
bioavailability
triterpenoids.
reviews
structural
modifications,
based
on
structure–activity
observations.
conclusion,
origin
some
semi-synthetic
derivatives
exert
significant
cytotoxic,
antiproliferative
chemopreventive
effects
can
be
beneficial
melanoma
treatment.
Recent
data
indicate
poor
solubility
water,
thus
bioavailability,
overcome
complexing
cyclodextrins,
or
nanoparticles
ethosomes,
making
these
compounds
further
ACS Omega,
Journal Year:
2023,
Volume and Issue:
8(33), P. 30630 - 30639
Published: Aug. 8, 2023
Phytochemical
study
of
the
ethyl
acetate
root
extract
Zygophyllum
album
has
resulted
in
isolation
a
new
saponin,
Zygo-albuside
D
(1),
along
with
two
known
compounds;
(3-O-[β-D-quinovopyranosyl]-quinovic
acid)
(2),
which
is
first
reported
root,
and
catechin
(3),
genus.
Their
chemical
structures
were
established
by
NMR
high-resolution
mass
spectrometry
(HRMS).
The
saponin
(1)
exhibited
promising
cytotoxicity
IC50
values
3.5
5.52
μM
on
A549
PC-3
cancer
cell
lines,
respectively,
compared
to
doxorubicin
9.44
11.39
respectively.
While
it
had
an
value
46.8
against
WISH
cells.
Investigating
apoptosis-induction,
compound
1
induced
total
apoptotic
death
lung
cells
32-fold;
21.53%
0.67%
untreated
control
Finally,
upregulated
pro-apoptotic
genes
downregulated
antiapoptotic
gene
using
expression
levels.
Compound
remarkable
CDK-2
target
inhibition
96.2%
117.6
nM
Roscovitine.
molecular
docking
further
confirmed
binding
affinity
as
CDK2
Bcl2
inhibitors
that
led
apoptosis
induction
Hence,
this
highlights
importance
design
anticancer
agent
specific
mechanisms.
Current Computer - Aided Drug Design,
Journal Year:
2024,
Volume and Issue:
21(1), P. 94 - 109
Published: Feb. 4, 2024
Background:
Uncaria
tomentosa
is
a
traditional
medicinal
herb
renowned
for
its
anti-inflammatory,
antioxidant,
and
immune-enhancing
properties.
In
the
realm
of
neurodegenerative
diseases
(NDDS),
CLASP
proteins,
responsible
regulating
microtubule
dynamics
in
neurons,
have
emerged
as
critical
players.
Dysregulation
proteins
associated
with
NDDS,
such
Alzheimer's,
Parkinson's,
Huntington's
diseases.
Consequently,
comprehending
role
NDDS
holds
promise
development
innovative
therapeutic
interventions.
Objectives:
The
objectives
research
were
to
identify
phytoconstituents
hydroalcoholic
extract
(HEUT),
evaluate
antioxidant
potential
through
vitro
free
radical
scavenging
assays
explore
interaction
using
silico
molecular
docking
studies.
Methods:
HPLC
LC-MS
techniques
used
quantify
phytochemicals
HEUT.
was
assessed
DPPH,
ferric
reducing
power
(FRAP),
nitric
oxide
(NO)
superoxide
(SO)
methods.
Interactions
between
conventional
quinovic
acid,
chlorogenic
epicatechin,
corynoxeine,
rhynchophylline
syringic
acid
studied
Auto
Dock
4.2.
Results:
HEUT
demonstrated
highest
concentration
derivatives.
exhibited
strong
radical-scavenging
activity
IC50
values
0.113
μg/ml
(DPPH)
9.51
μM
(FRAP).
It
also
suppressed
NO
production
by
47.1
±
0.37%
at
40
inhibited
77.3
0.69%
SO
generation.
Additionally,
revealed
NDDS.
Conclusion:
protein
suggest
promising
treating
linked
dysregulation.
Microscopy Research and Technique,
Journal Year:
2024,
Volume and Issue:
87(9), P. 2143 - 2153
Published: May 5, 2024
Abstract
In
the
Medicago
genus,
saponins
are
complex
mixtures
of
triterpene
pentacyclic
glycosides
extensively
studied
for
their
different
and
economically
relevant
biological
pharmaceutical
properties.
This
research
is
aimed
at
determining
first
time
tissue
cellular
localization
in
vegetative
organs
truncatula
,
a
model
plant
species
legumes,
by
histochemistry
transmission
electron
microscopy.
The
results
showed
that
present
mainly
palisade
mesophyll
layer
leaves,
whereas
stems
they
mostly
located
primary
phloem
subepidermal
cells
cortical
parenchyma.
root
tissue,
occur
secondary
region.
Transmission
microscopy
revealed
prominent
saponin
accumulation
within
leaf
stem
chloroplasts,
while
roots
found
vesicular
structures.
Our
demonstrate
feasibility
using
to
localize
M.
levels
provide
important
information
further
studies
on
biosynthesis
regulation
valuable
bioactive
agronomic
spp.,
such
as
alfalfa
(
sativa
L.).
Research
Highlights
genus
represents
rich
source
saponins,
one
most
interesting
groups
metabolites,
which
possess
pharmacological
Plant
great
importance
better
understand
functions,
biosynthetic
pathway,
regulatory
mechanisms.
We
elucidate
with
histochemical
studies.
Molecules,
Journal Year:
2024,
Volume and Issue:
29(16), P. 3794 - 3794
Published: Aug. 10, 2024
Plants
from
the
Amaranthaceae
family
are
a
source
of
oleanolic
acid
(OA)-type
saponins
with
cytotoxic
activity.
Two
known
OA-type
saponins,
calenduloside
E
and
chikusetsusaponin
IVa,
were
isolated
roots
Chenopodium
strictum
Roth.
Their
structures
confirmed
using
MS
NMR
techniques.
This
constitutes
inaugural
report
in
Ch.
strictum.
Both
structurally
similar
compounds,
momordin
Ic
OA,
compared
for
their
cytotoxicity
against
various
cancer
normal
cell
lines
(including
skin,
breast,
thyroid,
gastrointestinal,
prostate
panels).
effects
dose-
time-dependent,
varying
specific
line
compound
structure.
A
chemometric
approach
demonstrated
compounds
on
lines.
The
study
discusses
structure–activity
observations.
key
structural
elements
potent
activity
included
free
carboxyl
group
28COOH
sapogenin
structure
(OA)
presence
sugar
moiety.
monodesmosides
glucuronic
(GlcA)
at
C3
position
OA
generally
more
than
bidesmosides
or
alone.
addition
xylose
chain
modified
towards
cells
depending
line.
GlcA
(particularly
Ic)
represent
promising
avenue
further
investigation
as
potential
anticancer
agents.
Saudi Pharmaceutical Journal,
Journal Year:
2023,
Volume and Issue:
31(6), P. 1019 - 1028
Published: May 6, 2023
The
leaves
of
Zizyphus
spina-christi
(L.)
Willd
contain
several
compounds
exhibiting
different
pharmacologic
activities.
However,
studies
on
the
cytotoxic
activity
these
are
limited.We
aimed
to
investigate
and
isolate
with
selective
antitumor
effects
from
Z.
using
bioassay-guided
fractionation
methanol
extract.Powdered,
dried
were
subjected
extraction
fractionated
n-hexane,
chloroform,
ethyl
acetate,
n-butanol.
Fractions
positive
cytotoxicity
against
HeLa
THP-1
cell
lines
further
eluted
various
concentrations
organic
solvents.
Active
isolated
chromatographic
methods
their
chemical
structures
determined
extensive
spectroscopic
methods,
such
as
1D
NMR
(1H
NMR,
13C
DEPT),
2D
(COSY,
HMBC,
HMQC),
HRFAB-MS,
IR.
Furthermore,
evaluated
62
tumor
(including
THP-1)
in
addition
normal
bone
marrow
cells.The
chloroform
aqueous
fractions
showed
activity.
Two
successfully
named
"sidrin"
(13-β-hydroxy-lup-20(30)-ene-2,3-β-epoxy-28-carboxylate)
"sidroside"
(3-O-β-D-glucopyranosyl-(1-3)-α-L-arabinopyranosyl-jujubogenin-20-O-α-L-rhamnopyranoside).
Sidrin
exhibited
human
leukemia
(Hl-60,
RPMI-8226),
lung
cancer
(A549,
EKVX),
breast
(BT-549,
MDA-MB-231/ATCC),
colon
(KM12),
melanoma
(M14,
SK-MEL-5),
central
nervous
system
(CNS)
(SF-295)
lines,
selectivity
was
observed
Hl-60,
EKVX,
BT-549,
KM12,
SF-295
lines.
In
addition,
sidrin
more
active
than
sidroside
doxorubicin
Hl-60
EKVX
contrast,
had
a
similar
effect
BT-549
renal
(UO-31)
Sidroside
(CCRF-CEM,
MOLT-4),
(HOP-92,
NCI-H322M),
(MDA-MB-468),
(LOX
IMVI),
CNS
(SNB-19),
ovarian
(OVCAR-8),
(UO-31,
RXF
393),
prostate
(PC-3)
Both
(MDA-MB-231,
T-47D),
(HCC-2998,
HCT-116),
(OVCAR-3),
786-0,
SN
12C)
Normal
cells
unaffected
at
same
applied
cells.These
results
suggest
tumor-selective
sidroside.
Plants,
Journal Year:
2023,
Volume and Issue:
12(13), P. 2485 - 2485
Published: June 29, 2023
(1)
The
cytotoxicity
and
antioxidant
activity
of
different
fractions
as
well
the
pro-apoptotic
saponin
from
Eryngium
planum
L.
in
SKOV-3
was
investigated.
(2)
In
screening
studies,
six
on
examined
by
LDH
SRB
assays.
most
active
fractions—triterpenoid
saponins—were
selected
for
further
investigation.
To
determine
mechanism
fractions’
cytotoxicity,
their
ability
to
induce
apoptosis
via
Annexin
V
assay.
effect
caspase
3
measured
using
a
Caspase
Assay
Kit.
expression
84
apoptosis-related
genes
investigated
cancer
cells
exposed
roots.
radical
scavenging
capacity
determined
DPPH
(3)
pronounced
cytotoxic
effects
were
demonstrated
leaves
Those
chosen
treatment
cell
lines
with
saponins
obtained
roots
provoked
significant
increase
apoptotic
cells.
cells,
caused
upregulation
decrease
anti-apoptotic
genes.
activation
correlated
an
increased
DFFA
level
treated
phenolic
acids
shoots
(4)
best
our
knowledge,
current
study
is
first
demonstrate
that
barrigenol-type
triterpenoid
fraction
E.
inhibits
proliferation
induces
apoptosis,
which
may
be
regulated
mostly
specific
mitochondria-related
pathway.
