The role of amino acid metabolism during abiotic stress release

Plant Cell & Environment, Journal Year: 2019, Volume and Issue: 42(5), P. 1630 - 1644

Published: Jan. 11, 2019

Plant responses to abiotic stress include various modifications in amino acid metabolism. By using a hydroponic culture system, we systematically investigate modification profiles and the proteome of Arabidopsis thaliana leaves during initial recovery from low water potential or high salinity. Both treatments elicited oxidative leading biphasic response recovery. Degradation highly abundant proteins such as subunits photosystems ribosomes contributed an accumulation free acids. Catabolic pathways for several acids were induced indicating their usage alternative respiratory substrate compensate decreased photosynthesis. Our results demonstrate that rapid detoxification potentially detrimental Lys is priority period. The content Pro, which acts compatible osmolyte stress, was adjusted by balancing its synthesis catabolism both after treatments. production derived secondary metabolites up-regulated specifically period, our dataset also indicates increased rates precursor Overall, support tight relationship between metabolism responses.

Language: Английский

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Thermotolerance effect of plant growth-promoting Bacillus cereus SA1 on soybean during heat stress

BMC Microbiology, Journal Year: 2020, Volume and Issue: 20(1)

Published: June 22, 2020

Abstract Background Incidences of heat stress due to the changing global climate can negatively affect growth and yield temperature-sensitive crops such as soybean variety, Pungsannamul. Increased temperatures decrease crop productivity by affecting biochemical, physiological, molecular, morphological factors either individually or in combination with other abiotic stresses. The application plant growth-promoting endophytic bacteria (PGPEB) offers an ecofriendly approach for improving agriculture production counteracting negative effects stress. Results We isolated, screened identified thermotolerant B. cereus SA1 a bacterium that could produce biologically active metabolites, gibberellin, indole-3-acetic acid, organic acids. inoculation improved biomass, chlorophyll content, fluorescence plants under normal conditions 5 10 days. Heat increased abscisic acid (ABA) reduced salicylic (SA); however, markedly ABA SA. Antioxidant analysis results showed ascorbic peroxidase, superoxide dismutase, glutathione contents plants. In addition, decreased amino contents; they were inoculation. days shock protein (HSP) expression, GmHSP expression was observed after days; augmented response HSP expression. stress-responsive GmLAX3 GmAKT2 overexpressed SA1-inoculated may be associated reactive oxygen species generation, altered auxin stimuli, enhanced potassium gradients, which are critical Conclusion current findings suggest used mitigation damage commercialized biofertilizer only case found non-pathogenic.

Language: Английский

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Plant Metabolomics: An Overview of the Role of Primary and Secondary Metabolites against Different Environmental Stress Factors

Life, Journal Year: 2023, Volume and Issue: 13(3), P. 706 - 706

Published: March 6, 2023

Several environmental stresses, including biotic and abiotic factors, adversely affect the growth development of crops, thereby lowering their yield. However, e.g., drought, salinity, cold, heat, ultraviolet radiations (UVr), reactive oxygen species (ROS), trace metals (TM), soil pH, are extremely destructive decrease crop yield worldwide. It is expected that more than 50% production losses due to stresses. Moreover, these factors responsible for physiological biochemical changes in plants. The response different plant such stresses a complex phenomenon with individual features several species. In addition, it has been shown stimulate multi-gene responses by making modifications accumulation primary secondary metabolites. Metabolomics promising way interpret stress tolerance study metabolic profiling revealed types metabolites, amino acids, carbohydrates, phenols, polyamines, terpenes, etc, which accumulated Among all, as lipids glycine betaine, considered major contributing work osmolytes osmoprotectants plants from various factors. contrast, plant-derived phenolics, terpenoids, nitrogen-containing compounds (alkaloids), have no direct role Nevertheless, metabolites could play significant defense protecting herbivores, insects, pathogens. they can enhance resistance against Therefore, metabolomics practices becoming essential influential identifying phytochemicals part acclimation stimuli. Hence, an accurate metabolome analysis important understand basics physiology biochemistry. This review provides insight into current information related impact on variations sets metabolite levels explores how help

Language: Английский

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Plant responses to climate change: metabolic changes under combined abiotic stresses

Journal of Experimental Botany, Journal Year: 2022, Volume and Issue: 73(11), P. 3339 - 3354

Published: Feb. 21, 2022

Abstract Climate change is predicted to increase the frequency and intensity of abiotic stress combinations that negatively impact plants pose a serious threat crop yield food supply. Plants respond episodes combination by activating specific physiological molecular responses, as well adjusting different metabolic pathways, mitigate negative effects on plant growth, development, reproduction. synthesize wide range metabolites regulate many aspects growth responses stress. Although individual stresses have been studied extensively in species, recent efforts directed at understanding occur when factors are combined. In this review we examine studies metabolomic changes under suggest new avenues for development combination-resilient crops based breeding targets.

Language: Английский

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Potential use of Bacillus spp. as an effective biostimulant against abiotic stresses in crops—A review

Current Research in Biotechnology, Journal Year: 2023, Volume and Issue: 5, P. 100128 - 100128

Published: Jan. 1, 2023

Environmental (abiotic) stresses significantly threaten the worldwide crop production and food security. Rapid, drastic changes in global climate have exacerbated such for crops. Plant-associated bacteria been shown to enhance stress resistance cope with negative impacts of various abiotic through induction mechanisms. In soil, rhizosphere endosphere plants, Bacillus genus is a predominant bacterial genus. Members this genus, which are tremendously diverse both metabolically genetically, survive long time under unfavorable environmental conditions due their ability form long-lived, stress-tolerant spores. spp. secrete several metabolites that trigger plant growth plants' tolerance biotic stresses. Some species available commercially as phytostimulants, biopesticides, biofertilizers. Due functional versatility, one most widely used agro-biotech industry. However, potential has not yet sufficiently realized, transferring technology related from lab environment real world applications field needs be emphasized. A better understanding mechanisms action beneficial needed development products support green biotechnology agriculture industries. This report comprehensively reviews (e.g., salinity, drought, inorganic organic pollutant toxicity, nutritional imbalance, low–high temperatures, waterlogging) -stressed discusses potentials new biotechnological implications, highlighting gaps remain explored improve expand on Bacillus-based biostimulants.

Language: Английский

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The multifaceted roles of Arbuscular Mycorrhizal Fungi in peanut responses to salt, drought, and cold stress

BMC Plant Biology, Journal Year: 2023, Volume and Issue: 23(1)

Published: Jan. 16, 2023

Arbuscular Mycorrhizal Fungi (AMF) are beneficial microorganisms in soil-plant interactions; however, the underlying mechanisms regarding their roles legumes environmental stress remain elusive. Present trials were undertaken to study effect of AMF on ameliorating salt, drought, and cold peanut (Arachis hypogaea L.) plants. A new product combined with Rhizophagus irregularis SA, clarus BEG142, Glomus lamellosum ON393, Funneliformis mosseae BEG95 (1: 1: 1, w/w/w/w) was inoculated physiological metabolomic responses AMF-inoculated non-inoculated plants comprehensively characterized, respectively.AMF-inoculated exhibited higher plant growth, leaf relative water content (RWC), net photosynthetic rate, maximal photochemical efficiency photosystem II (PSII) (Fv/Fm), activities antioxidant enzymes, K+: Na+ ratio while lower electrolyte conductivity (REC), concentration malondialdehyde (MDA), accumulation reactive oxygen species (ROS) under stressful conditions. Moreover, structures chloroplast thylakoids mitochondria less damaged by these stresses. Non-targeted metabolomics indicated that altered numerous pathways associated organic acids amino acid metabolisms roots both normal-growth conditions, which further improved osmolytes data.This provides a promising demonstrates this combination could enhance tolerance through improving protecting photosystem, enhancing system, regulating osmotic adjustment.

Language: Английский

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Soil Salinity, a Serious Environmental Issue and Plant Responses: A Metabolomics Perspective

Metabolites, Journal Year: 2021, Volume and Issue: 11(11), P. 724 - 724

Published: Oct. 22, 2021

The effects of global warming have increasingly led to devastating environmental stresses, such as heat, salinity, and drought. Soil salinization is a serious issue results in detrimental abiotic stress, affecting 7% land area 33% irrigated lands worldwide. proportion arable facing salinity expected rise due increasing climate change fuelled by anthropogenic activities, exacerbating the threat food security for exponentially growing populace. As sessile organisms, plants evolutionarily developed mechanisms that allow ad hoc responses stress. orchestrated include signalling cascades involving phytohormones, kinases, reactive oxygen species (ROS), calcium regulatory networks. pillar systems biology approach, metabolomics allows comprehensive interrogation biochemistry deconvolution molecular involved plant salinity. Thus, this review highlights soil points negative impacts on plants. Furthermore, summarises regulating tolerance molecular, cellular, biochemical levels with focus perspectives. This critical synthesis current literature an opportunity revisit models regarding invitation further fundamental research novel actionable insights.

Language: Английский

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Differential Contribution of P5CS Isoforms to Stress Tolerance in Arabidopsis

Frontiers in Plant Science, Journal Year: 2020, Volume and Issue: 11

Published: Sept. 25, 2020

Proline accumulation is a widespread response of plants to salt stress as well drought and cold stress. In most plant species, two isoforms pyrroline-5-carboxylate synthetase (P5CS) catalyze the first step in proline biosynthesis from glutamate. Arabidopsis, these differ their spatial temporal expression patterns, suggesting sub-functionalization. P5CS1 has been identified major contributor stress-induced accumulation, whereas P5CS2 considered important for embryo development growth. contrast previous results, our analysis P5CS1- P5CS2-GFP fusion proteins indicates that both enzymes were exclusively localized cytosol. The comparison susceptibility p5cs1 p5cs2 mutants infection with Pseudomonas syringae provided novel information on contribution P5CS tolerance. agreement studies, salt-stressed accumulated very little proline, indicating contributed more its impact tolerance was rather weak. Germination establishment impaired under ambient conditions, further supporting growth development, smaller than P5CS1. or wildtype plants, only weakly affected by sudden exposure high NaCl concentration. These findings show content, which intermediate leaves mutants, not directly correlated experiments. rosettes NaCl-exposed nearly no Na+ observed, showed neither chlorosis nor reduction photosynthesis. Based data, we suggest function P5CS2-mediated synthesis regulating thereby

Language: Английский

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Plant metabolomics in biotic and abiotic stress: a critical overview

Phytochemistry Reviews, Journal Year: 2021, Volume and Issue: 21(2), P. 503 - 524

Published: Nov. 9, 2021

Language: Английский

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Extending thermotolerance to tomato seedlings by inoculation with SA1 isolate of Bacillus cereus and comparison with exogenous humic acid application

PLoS ONE, Journal Year: 2020, Volume and Issue: 15(4), P. e0232228 - e0232228

Published: April 30, 2020

Heat stress is one of the major abiotic stresses that impair plant growth and crop productivity. Plant growth-promoting endophytic bacteria (PGPEB) humic acid (HA) are used as bio-stimulants ecofriendly approaches to improve agriculture production counteract negative effects heat stress. Current study aimed analyze effect thermotolerant SA1 an isolate Bacillus cereus HA on tomato seedlings. The results showed combine application SA1+HA significantly improved biomass chlorophyll fluorescence plants under normal conditions. increased abscisic (ABA) reduced salicylic (SA) content; however, combined markedly ABA SA. Antioxidant enzymes activities revealed treated exhibited levels ascorbate peroxidase (APX), superoxide dismutase (SOD), glutathione (GSH). In addition, amino contents; acids were with co-application SA1+HA. Similarly, inductively-coupled plasma mass-spectrometry higher iron (Fe+), phosphorus (P), potassium (K+) uptake during relative expression SlWRKY33b autophagy-related (SlATG5) genes, whereas augmented response SlATG5 expression. stress-responsive transcription factor (SlHsfA1a) high-affinity transporter (SlHKT1) upregulated in SA1+HA-treated plants. conclusion, current findings suggest can be for mitigation damage commercialized a biofertilizer.

Language: Английский

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