bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 16, 2024
Abstract
The
aim
of
this
study
was
to
explore
the
viability
dual
production
polyhydroxybutyrate
(PHB)
and
exopolysaccharides
(EPS)
by
seven
microbiomes
rich
in
cyanobacteria.
Our
initial
experiments
involved
screen
for
EPS-producing
candidates
examine
impact
salinity
acetate
on
EPS
synthesis.
Salinity’s
known
influence
acetate’s
role
enhancing
PHB
guided
our
parameter
selection.
Surprisingly,
neither
introduction
an
external
carbon
source
(acetate)
nor
exposure
abiotic
stressor
(salt)
significantly
altered
synthesis
rates,
which
ranged
from
25
150
mg·L
-1
,
or
its
composition,
with
glucose
being
dominant
sugar
component.
Scaling
up
a
3
L
photobioreactor,
we
achieved
simultaneous
biopolymer
production,
reaching
205
87
PHB.
Additionally,
presence
uronic
acid
facilitated
biomass
flocculation,
streamlining
separation
process,
potentially
reducing
associated
time
costs.
Deleted Journal,
Journal Year:
2024,
Volume and Issue:
1(1)
Published: Sept. 2, 2024
Bioplastics
derived
from
polyhydroxyalkanoates
(PHAs)
are
among
the
promising
substitutes
for
unsustainable
petroleum-based
polymers.
PHA-based
polymers
demonstrate
superior
chemical
and
physical
properties,
such
as
hydrophobicity,
insolubility
in
water,
iso-tacticity,
UV
resistance,
hydrolysis
absolute
biodegradability.
Compared
with
conventional
plastics,
bioplastics
more
beneficial
due
to
their
reduced
carbon
footprint,
energy
efficiency,
biodegradability,
biocompatibility,
have
hence
revolutionized
polymer
industry.
However,
further
research
is
needed
explore
novel
strategies
overcome
limitations,
decreasing
water
absorption
brittleness,
while
increasing
crystallization
ability
thermal
degradation
temperature.
These
constraints
can
be
addressed
by
supplementing
bioplastic
synthesis
process
reinforcements
plasticizers.
The
development
adoption
of
biopolymers
an
environmentally
friendly
economically
viable
synthetic
plastics
imperative,
considering
degree
subsequent
exhaustion
petrochemical
supplies
worldwide
environmental
contamination
instigated
industrial
production
plastics.
goal
this
appraisal
provide
in-depth
account
most
recent
advancements
generation
various
wastewater
streams
via
use
microalgae,
harvesting
technologies.
microalgae
higher
quality
made
polymeric
biomolecules
include
based
on
cellulose,
starch,
proteins,
PHA,
polyhydroxybutyrate
(PHB),
polyethylene
(PE),
polylactic
acid
(PLA),
poly
vinyl
chloride
(PVC).
Various
types
manufacturing
methodologies
also
been
highlighted
researchers
capitalists
alike
investigate
ways
harness
these
renewable
resources
sustainable
bioplastics.
Additionally,
innovations,
challenges,
potential
possibilities
future,
life
cycle
evaluations
addressed.
IntechOpen eBooks,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 14, 2025
Microalgae
are
photosynthetic
microorganisms
that
produce
a
wide
range
of
biocompounds,
such
as
proteins,
omega-3
fatty
acids
or
pigments
like
astaxanthin;
with
various
applications
in
the
pharmaceutical,
cosmetic,
bioenergy
and
food
sectors.
This
chapter
provides
an
overview
compounds
molecules
synthesized
by
microalgae,
ranging
from
polysaccharides
to
vitamins,
minerals
other
secondary
metabolites.
Additionally,
reviews
key
biological
aspects
influence
production
including
strain
selection,
improvement
cultivation
conditions,
well
biotechnological
factors
necessary
optimize
processing
these
compounds,
system,
extraction
purification.
Lastly,
presents
main
commercially
relevant
microalgae-derived
emphasizing
most
notable
microalgae-based
products
currently
being
developed
global
market.
New Biotechnology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 1, 2025
The
aim
of
this
study
was
to
explore
the
viability
dual
production
poly(3-hydroxybutyrate)
(PHB)
and
exopolysaccharides
(EPS)
by
seven
microbiomes
rich
in
cyanobacteria.
Our
initial
experiments
involved
screen
for
EPS-producing
candidates
examine
impact
salinity
acetate
on
EPS
synthesis.
Salinity's
known
influence
acetate's
role
enhancing
PHB
guided
our
parameter
selection.
Surprisingly,
neither
introduction
an
external
carbon
source
(acetate)
nor
exposure
abiotic
stressor
(salt)
significantly
altered
synthesis
rates,
which
ranged
from
25
150mg·L-1,
or
its
composition,
with
glucose
being
dominant
sugar
component.
Scaling
up
a
3L
photobioreactor,
we
achieved
simultaneous
biopolymer
production,
reaching
205mg·L-1
87mg·L-1
PHB.
Additionally,
presence
uronic
acid
facilitated
biomass
flocculation,
streamlining
separation
process,
potentially
reducing
associated
time
costs.
Polymers,
Journal Year:
2025,
Volume and Issue:
17(2), P. 197 - 197
Published: Jan. 14, 2025
Crude
glycerol
(CG),
a
major
biodiesel
production
by-product,
is
the
focus
of
ongoing
research
to
convert
it
into
polyhydroxyalkanoate
(PHA).
However,
few
bacterial
strains
are
capable
efficiently
achieving
this
conversion.
Here,
10
PHA-producing
were
isolated
from
various
media.
Among
them,
Burkholderia
sp.
Oh_219
exhibited
highest
polyhydroxybutyrate
(PHB)
and
was
therefore
characterized
further.
demonstrated
significant
tolerance
growth
inhibitors
in
CG
metabolized
fatty
acids
present
as
impurities
CG.
Furthermore,
strain
genetically
engineered
using
phaCBP-M-CPF4
phaJPa
enable
acid-based
poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)
(PHBHHx),
component
The
resulting
produced
PHBHHx
containing
1.0–1.3
mol%
3HHx
Further
supplementation
with
capric
lauric
increased
molar
fraction
9.7%
18%,
respectively.
In
5
L
fermenter,
15.3
g/L
PHB
29.6
biomass
two-stage
fermentation
system.
This
yield
reported
for
PHA
by
spp.
Additionally,
had
lower
melting
point
than
that
pure
fructose.
Taken
together,
promising
new
candidate
producing
Chemosphere,
Journal Year:
2025,
Volume and Issue:
373, P. 144181 - 144181
Published: Feb. 4, 2025
The
production
of
polyhydroxyalkanoate
(PHA)
using
CO2
through
hydrogen-oxidizing
bacteria
under
safe,
non-explosive
conditions
is
making
impressive
strides.
present
study
aimed
to
evaluate
and
demonstrate
the
growth
productivity
PHA
by
Cupriavidus
sp.
CY-1
different
conditions,
thereby
providing
critical
data
for
practical
applications.
experimental
results
highlighted
efficiency
strain
in
biosynthesis,
achieving
a
rate
11.87
g
L-1,
which
corresponds
90.6%
yield
when
fermenting
gaseous
substrate
composed
H2
(70%),
O2
(20%),
(10%).
also
examined
including
concentrations
3.8%
(v/v)
at
6.5%
(v/v).
Furthermore,
impact
CO
(30%
higher)
was
assessed,
revealing
detrimental
effect
on
production.
Notably,
addition
Tween
80
significantly
enhanced
productivity.
effective
utilization
has
confirmed
poly[(R)-3-hydroxybutyrate]
(PHB)
as
valuable
derived
form
PHA.
By
implementing
two-step
treatment
with
valeric
acid,
we
successfully
produced
P(3HB-co-3HV)
(PHBV)
concentration
1.47
L-1.
This
achievement
highlights
potential
enhance
innovative
strategies.
examination
phaC
gene
expression
levels
facilitated
accurate
predictions
use
from
trichloroethylene
(TCE)
biodegradation
faced
concentration-related
challenges;
however,
higher
achieved
phenol
biodegradation,
1200
mg
indicate
substantial
efficient
Polymers,
Journal Year:
2024,
Volume and Issue:
16(19), P. 2773 - 2773
Published: Sept. 30, 2024
Although
efforts
have
been
undertaken
to
produce
polyhydroxyalkanoates
(PHA)
with
various
monomers,
the
low
yield
of
PHAs
because
complex
metabolic
pathways
and
inhibitory
substrates
remains
a
major
hurdle
in
their
analyses
applications.
Therefore,
we
investigated
feasibility
mass
production
containing
5-hydroxyvalerate
(5HV)
using
δ-valerolactone
(DVL)
without
any
pretreatment
along
addition
plant
oil
achieve
enough
biomass.
We
identified
that
PhaC
