Springer eBooks, Journal Year: 2020, Volume and Issue: unknown, P. 309 - 343
Published: Jan. 1, 2020
Language: Английский
Frontiers in Genome Editing, Journal Year: 2021, Volume and Issue: 3
Published: Sept. 3, 2021
The development of gene-editing technology holds tremendous potential for accelerating crop trait improvement to help us address the need feed a growing global population. However, delivery and access tools host genome subsequent recovery successfully edited plants form significant bottlenecks in application new plant breeding technologies. Moreover, methods most suited achieve desired outcome vary substantially, depending on species' genotype targeted genetic changes. Hence, it is importance develop improve multiple strategies regeneration order be able approach each from various angles. use transient transformation protoplasts one such strategy that carries unique advantages challenges. Here, we will discuss protoplast technologies review pertinent literature successful regeneration.
Language: Английский
Citations
110
Planta, Journal Year: 2022, Volume and Issue: 255(3)
Published: Feb. 15, 2022
Language: Английский
Citations
78
Life, Journal Year: 2023, Volume and Issue: 13(3), P. 780 - 780
Published: March 14, 2023
Plants are constantly faced with biotic or abiotic stress, which affects their growth and development. Yield reduction due to stresses on economically important crop species causes substantial economic loss at a global level. Breeding for stress tolerance create elite superior genotypes has been common practice many decades, plant tissue culture can be an efficient cost-effective method. Tissue is valuable tool develop tolerance, screen elucidate physiological biochemical changes during stress. In vitro selection carried out under controlled environment conditions in confined spaces highly effective cheaper maintain. This review emphasizes the relevance of screening major stresses, drought, salinity, development disease resistance. Further emphasis given metal hyperaccumulators transgenic technological applications tolerance.
Language: Английский
Citations
26
Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 74(13), P. 3821 - 3832
Published: May 23, 2023
Abstract Protoplasts, which are plant cells with their cell walls removed, have been used for decades in research and instrumental genetic transformation the study of various aspects physiology genetics. With advent synthetic biology, these individualized fundamental to accelerate ‘design–build–test–learn’ cycle, is relatively slow research. Despite potential, challenges remain expanding use protoplasts biology. The capacity individual hybridize form new varieties, regenerate from single cells, creating individuals features underexplored. main objective this review discuss biology highlight exploiting protoplast technologies ‘age biology’.
Language: Английский
Citations
22
Plants, Journal Year: 2023, Volume and Issue: 12(5), P. 1060 - 1060
Published: Feb. 27, 2023
Species of the family Apiaceae occupy a major market share but are hitherto dependent on open pollinated cultivars. This results in lack production uniformity and reduced quality that has fostered hybrid seed production. The difficulty flower emasculation led breeders to use biotechnology approaches including somatic hybridization. We discuss protoplast technology for development hybrids, cybrids in-vitro breeding commercial traits such as CMS (cytoplasmic male sterility), GMS (genetic sterility) EGMS (environment-sensitive genic sterility). molecular mechanism(s) underlying its candidate genes also discussed. Cybridization strategies based enucleation (Gamma rays, X-rays UV rays) metabolically arresting protoplasts with chemicals iodoacetamide or iodoacetate reviewed. Differential fluorescence staining fused routinely used can be replaced by new tagging using non-toxic proteins. Here, we focused initial plant materials tissue sources isolation, various digestion enzyme mixtures tested, understanding cell wall re-generation, all which intervene hybrids regeneration. Although there no alternatives hybridization, discussed emerging, viz., robotic platforms, artificial intelligence, recent programs trait identification selection.
Language: Английский
Citations
17
Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 207, P. 108383 - 108383
Published: Jan. 23, 2024
Language: Английский
Citations
8
Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2020, Volume and Issue: 143(3), P. 693 - 707
Published: Oct. 21, 2020
Abstract In this study we report the development of effective in vitro systems for a medicinal plant Nigella damascena L. comprising: (1) callus induction, (2) somatic embryogenesis cultures with subsequent regeneration, and (3) isolation regeneration callus-derived protoplasts. Callus was achieved on 83–100% hypocotyl cotyledon explants, whereby Murashige Skoog medium (MS) supplemented 3 mg L −1 6-benzylaminopurine 0.5 α -naphthaleneacetic acid (NAA; BN medium) more advantageous than MS kinetin NAA (KN medium). Histological observations calli revealed presence embryogenic zones from which embryos developed hormone-free medium. Plant observed 76–95% calli. A high capacity to form maintained long-lasting cultures, i.e. even 2 year old callus. The obtained also good source tissue protoplast isolation. By applying mixture cellulase pectolyase, acceptable yield viable protoplasts achieved, especially hypocotyl-derived Protoplasts embedded an alginate matrix cultured modified Kao Michayluk media re-constructed their cell wall re-entered mitotic divisions. About 30% small aggregates formed microcalli, which, after release alginate, proliferated continuously KN media, irrespective variant used as source. Somatic embryo formation were successful media. An system N. has been is being reported first time.
Language: Английский
Citations
44
Frontiers in Plant Science, Journal Year: 2020, Volume and Issue: 11
Published: Oct. 28, 2020
Seed yield, a major determinant for the commercial success of grain crops, critically depends on pollen viability, which is dramatically reduced by environmental stresses, such as drought, salinity, and extreme temperatures. Salinity, in particular, problem crop yield known to affect about 20% all arable land cause huge economic losses worldwide. Flowering plants are particularly sensitive stress during sexual reproduction, even short exposure stressing conditions can severely hamper reproductive success, thus reduce yield. Since proline required fertility accumulates plant tissues response different abiotic role protection under salt be envisaged. In this perspective, we analyze old new data evaluate importance development saline conditions, discuss possibility raising levels grains biotechnological strategy stabilize seed presence stress. The overall confirm that necessary preserve limit loss stressful conditions. However, at present, have not enough conclude whether or over wildtype effectively ameliorate further work still required.
Language: Английский
Citations
33
Agronomy, Journal Year: 2021, Volume and Issue: 11(4), P. 735 - 735
Published: April 10, 2021
Polyamines (PAs) are organic molecules that found in plants and animals. In plants, they involved the regulation of cellular growth, apoptosis, rooting, flower development, stress responses. The effect exogenously applied polyamines on development Brassica oleracea L. var. capitata protoplast cultures was studied. Protoplasts were isolated from hypocotyls 2-week-old seedlings three accessions cultured liquid media supplemented with putrescine (Put), spermidine (Spd), spermine (Spm) at concentrations 0 (control), 10, 20, 40 µM. very early culture (24 48 h), reactive oxygen species levels (ROS) live cells monitored using a fluorescent probe. Put- Spd-treated protoplasts exhibited lower fluorescence intensities, which corresponded to ROS accumulation as compared PA-free control. viability affected by type polyamine rather than its concentration. Put Spd had beneficial mitotic activity cells, observed all tested accessions. highest frequency shoot organogenesis (21%) obtained microcalli derived medium 10 µM Put. Analysis ploidy level regenerants showed vast majority diploids. Our results demonstrated PAs maintained B. alleviating oxidative stimulating activity, further plant regeneration process.
Language: Английский
Citations
25
Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)
Published: May 4, 2022
Abstract Soil salinization is a growing problem for agriculture worldwide and carrot one the most salt-sensitive vegetable species. However, some varieties are capable of withstanding high salt concentrations due to unknown genetic physiological mechanisms. The aim this work was reveal protecting mechanisms against osmotic ionic stresses that contribute tolerance in carrot. For purpose, changes biochemical traits soil salinity occurring salt-tolerant plants were determined. obtained results showed variety partially determined constitutively, however, exposition saline triggered response more evident root than leaves. noticeable increase content osmoprotective proline other low molecular antioxidants such as glutathione ascorbic acid, decrease ratio reduced oxidized forms. These imply an efficient operation ascorbate–glutathione cycle together with activity antioxidative enzymes peroxidases, indicate on induction associated mainly protection excessive reactive oxygen
Language: Английский
Citations
19