Plant Methods,
Journal Year:
2023,
Volume and Issue:
19(1)
Published: Dec. 15, 2023
Abstract
Background
Tea
is
the
most
popular
beverage
worldwide
second
only
to
water.
Its
demand
tremendously
rising
due
increased
awareness
of
its
medicinal
importance.
The
quality
and
uses
tea
depend
on
tea-types
which
are
mainly
three
types
including
China,
Assam
Cambod
type
having
distinct
compositions
secondary
metabolites.
Huge
variation
in
metabolites
different
cultivars
limited
successful
application
various
approaches
used
for
trait
improvement.
efficiency
a
protocol
isolation
protoplast
specific
plants.
existing
protoplast-isolation
protocols
[which
were
optimized
(China
type)
Chinese
grown
China]
found
ineffective
types/cultivars
India
type/cultivar
variability.
Therefore,
optimization
essential
tea-types/cultivars
India,
as
it
largest
producer
black
tea.
Here,
efforts
made
develop
an
efficient
from
all
major
(China,
types)
also
tender
leaves
obtained
field-grown,
hydroponically-grown
tissue
culture-grown
Results
Developed
was
effective
leaf
Moreover,
effectively
worked
cultivated
India.
digestion
with
3%
cellulase
R-10,
0.6%
macerozyme,
1%
hemicellulase
4%
polyvinylpyrrolidone
12
h
at
28ºC
yielded
approximately
3.8–4.6
×
10
7
protoplasts
per
gram
fresh
80–95%
viability
selected
cultivars,
culture
plant
material
appropriate
isolation.
Conclusions
In
conclusion,
we
reported
Cambod)
tender-leaf
findings
expected
contribute
genetic
improvement
traits
widely.
Plants,
Journal Year:
2024,
Volume and Issue:
13(2), P. 327 - 327
Published: Jan. 22, 2024
Precise
knowledge
of
all
aspects
controlling
plant
tissue
culture
and
in
vitro
regeneration
is
crucial
for
biotechnologists
their
correlated
industry,
as
there
increasing
demand
this
scientific
knowledge,
resulting
more
productive
resilient
plants
the
field.
However,
development
application
cell
techniques
are
usually
based
on
empirical
studies,
although
some
data-driven
models
available.
Overall,
success
dependent
several
factors
such
available
nutrients,
endogenous
auxin
synthesis,
organic
compounds,
environment
conditions.
In
review,
most
important
described
one
by
one,
with
practical
recommendations
basic
research
physiology
sharing
our
experience
from
over
20
years
The
main
aim
to
help
new
increase
impact
industry
worldwide.
Plant Biotechnology Journal,
Journal Year:
2023,
Volume and Issue:
22(1), P. 19 - 36
Published: Oct. 4, 2023
Summary
Prime
editing
(PE)
technology
utilizes
an
extended
prime
guide
RNA
(pegRNA)
to
direct
a
fusion
peptide
consisting
of
nCas9
(H840)
and
reverse
transcriptase
(RT)
specific
location
in
the
genome.
This
enables
installation
base
changes
at
targeted
site
using
portion
pegRNA
through
RT
activity.
The
resulting
product
reaction
forms
3′
flap,
which
can
be
incorporated
into
genomic
series
biochemical
steps
involving
DNA
repair
synthesis
pathways.
PE
has
demonstrated
its
effectiveness
achieving
almost
all
precise
gene
editing,
such
as
conversions
(all
types),
sequence
insertions
deletions,
chromosomal
translocation
inversion
long
insertion
safe
harbour
sites
within
In
plant
science,
could
serve
groundbreaking
tool
for
allowing
creation
desired
alleles
improve
crop
varieties.
Nevertheless,
application
encountered
limitations
due
efficiency
constraints,
particularly
dicotyledonous
plants.
this
review,
we
discuss
step‐by‐step
mechanism
PE,
shedding
light
on
critical
aspects
each
step
while
suggesting
possible
solutions
enhance
efficiency.
Additionally,
present
overview
recent
advancements
future
perspectives
research
specifically
focused
plants,
examining
key
technical
considerations
applications.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(23), P. 16892 - 16892
Published: Nov. 29, 2023
Protoplasts,
derived
from
plant
cells,
exhibit
remarkable
totipotency
and
hold
significant
value
across
a
wide
spectrum
of
biological
biotechnological
applications.
These
versatile
applications
encompass
protein
subcellular
localization
interaction
analysis,
gene
expression
regulation,
functional
characterization,
editing
techniques,
single-cell
sequencing.
Protoplasts'
usability
stems
their
inherent
accessibility
ability
to
efficiently
incorporate
exogenous
genes.
In
this
review,
we
provide
comprehensive
overview,
including
details
on
isolation
procedures
influencing
factors,
purification
viability
assessment
methodologies,
the
utilization
protoplast
transient
system.
The
aim
is
overview
current
offer
valuable
insights
into
establishment
systems
in
diverse
range
species,
thereby
serving
as
resource
for
science
community.
Journal of Experimental Botany,
Journal Year:
2023,
Volume and Issue:
74(13), P. 3787 - 3790
Published: July 18, 2023
Plant
synthetic
biology
allows
for
the
reprogramming
of
biological
systems
to
promote
sustainable
agriculture.It
has
a
range
applications,
including
improving
nutritional
quality,
engineering
resilient
crops,
creating
new
materials,
and
producing
various
bioproducts.In
addition,
plant
provides
way
answer
fundamental
questions
in
biology.This
Special
Issue
presents
collection
review
articles
that
delve
into
current
future
state
biology.
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 24, 2025
Abstract
The
nucleus
is
delimited
by
the
nuclear
envelope
(NE),
where
pore
complexes
(NPCs)
are
embedded.
While
this
complex
has
been
well
studied
in
vertebrates,
yeast,
and,
more
recently,
algae,
situ
structural
data
of
higher
plants
still
missing.
Here,
we
show
that
many
individual
nucleoporins
Arabidopsis
thaliana
and
human
present
high
similarity.
We
report
a
first
plant
NPC
structure,
derived
from
A.
root
protoplasts
using
cryo
electron
tomography,
subtomogram
averaging
homology-based
integrative
modeling.
model
based
on
predicted
models
NUPs
identified
mass
spectrometry.
scaffold
exhibits
differences
diameter
height
to
C.
reinhardtii,
while
sharing
some
features
with
H.
sapiens
NPCs.
Notably,
observed
contains
NUP155
connectors
like
NPC,
which
may
explain
difference
when
compared
reinhardtii
NPC.
