Tinospora
cordifolia
(Family
Menispermaceae),
commonly
known
as
"Guduchi,"
is
an
extensively
used
medicinal
plant
in
modern
well
traditional
Ayurvedic
systems
of
medicine.
The
constitutive
occurrence
various
bioactive
constituents
such
terpenes,
alkaloids,
glycosides,
aliphatic
compounds,
and
flavonoids
attributes
to
its
inexplicable
efficacy
towards
chronic
ailments
due
antidiabetic,
anti-inflammatory,
hepatoprotective,
immunomodulatory,
antiperiodic,
antileprotic,
antispasmodic,
antiarthritic,
antioxidant,
antistress,
antimalarial,
antineoplastic
activities.
whole
parts
viz.
roots,
stem,
leaves
act
a
repository
these
important
are
utilized
the
preparation
pharmaceutical,
nutraceutical,
cosmeceutical
formulations.
A
scan
published
literature
survey
on
T.
clearly
indicates
that
array
reviews
available
phytochemical
pharmacological
activities
this
plant,
but
systematic
compilation
for
conventional
nonconventional
mode
propagation,
conservation,
strategies
sustainable
production
metabolites
still
lacking.
Hence,
present
review
attempt
direction
focused
understanding
different
means
propagation
alternative
platform
strategizing
conservation
plant.
critical
evaluation
future
implications
breakthrough
commercialization
also
discussed.
Future Journal of Pharmaceutical Sciences,
Journal Year:
2024,
Volume and Issue:
10(1)
Published: May 22, 2024
Abstract
Background
Thalictrum
foliolosum
,
a
member
of
the
Ranunculus
family,
is
recognized
for
its
therapeutic
potential
in
addressing
gastric
issues,
dyspepsia,
tooth
pain,
abdominal
colic
and
piles.
The
diverse
array
secondary
metabolites
present
plant
contributes
to
these
applications.
This
study
aims
uncover
quantify
bioactive
found
unexplored
leaves,
stems,
roots
T.
.
Additionally,
we
also
aimed
evaluate
antibacterial
activity
MIC
values
extracts
against
panel
pathogenic
bacteria,
such
as
strains,
including
Escherichia
coli,
Pseudomonas
aeruginosa,
Streptococcus
mutant
Staphylococcus
aureus
Result
HPLC
analysis
suggested
all
examined
compounds
were
significantly
more
root
parts
plant.
To
determine
antimicrobial
different
result
chloroform
fraction
most
effective
with
variable
potency
each
pathogen
at
25–100
µg/ml
which
indicated
rich
content
berberine
this
fraction.
Minimum
(121.26
µg/mL)
was
supported
results.
Fatty
acid
methyl
ester
by
gas
chromatography
revealed
that
stem
contained
high
levels
fatty
acids,
palmitic
acid,
stearic
linolenic
have
properties.
Conclusion
various
strongly
supports
plant's
widespread
use
folk
medicine
treatment
chronic
diseases
adulterants
associated
medicinal
species.
Plants,
Journal Year:
2022,
Volume and Issue:
11(12), P. 1614 - 1614
Published: June 20, 2022
Efficient
methods
for
callus
induction
and
the
high-frequency
plant
regeneration
of
Ruta
chalepensis
L.
were
established,
phytochemical
potential
antioxidant
activity
a
donor
plant,
ex-vitro-established
micropropagated
plants,
also
studied.
Yellowish-green
was
induced
with
frequency
97.8%
from
internode
shoot
segments
growing
in
soil
botanical
garden
cultured
on
Murashige
Skoog
(MS)
medium
containing
10
μM
2,4-D
(2,4-dichlorophenoxyacetic
acid)
1
BA
(6-benzyladenine).
Adventitious
shoots
regenerated
yellowish-green
MS
5.0
(BA)
1.0
1-naphthaleneacetic
acid
(NAA),
98.4%
maximum
54.6
an
average
length
4.5
cm
after
8
weeks.
The
rooted
IBA
(indole-3-butyric
successfully
transferred
to
ex
vitro
conditions
pots
normal
soil,
95%
survival
rate.
amounts
alkaloids,
phenolics,
flavonoids,
tannins,
plants
higher
than
callus.
highest
contents
hesperidin
rutin
(93.3
55.9
µg/mg,
respectively)
found
compared
those
obtained
(91.4
31.0
(59.1
21.6
respectively).
genetic
uniformity
appraised
by
ISSR
markers
plant.
This
is
first
report
describing
callus-mediated
regeneration,
as
well
production
phenolic
compounds
activities
R.
chalepensis,
which
might
be
alternative
technique
mass
propagation
synthesis
bioactive
such
rutin.
Plants,
Journal Year:
2021,
Volume and Issue:
10(12), P. 2820 - 2820
Published: Dec. 20, 2021
Ruta
chalepensis
L.,
an
evergreen
shrub
in
the
citrus
family,
is
well-known
around
world
for
its
essential
oils
and
variety
of
bioactivities,
indicating
potential
medicinal
applications.
In
this
study,
we
investigated
effect
different
culture
conditions,
including
plant
growth
regulators,
media
types,
pH
medium,
carbon
sources,
on
vitro
regeneration
from
nodal
explants
R.
chalepensis.
Following
8
weeks
culture,
highest
percentage
(96.3%)
maximum
number
shoots
(40.3
shoot/explant)
with
a
length
4.8
cm
were
obtained
Murashige
Skoog
(MS)
medium
at
5.8,
supplemented
3.0%
sucrose
5.0
µM
6-Benzyladenine
(BA)
combination
1.0
1-naphthaleneacetic
acid
(NAA).
For
rooting,
individually
harvested
shootlets
transferred
½
MS
(half-strength)
IAA
(indole-3-acetic
acid),
IBA
(indole
3-butyric
or
NAA,
best
response
terms
root
induction
(91.6%),
roots
(5.3),
mean
(4.9
cm)
was
achieved
0.5
after
6
weeks.
An
average
95.2
percent
healthy,
regenerated
plantlets
survived
being
transplanted
into
potting
soil,
that
they
effectively
hardened.
DNA
assays
(PCR-based
markers)
such
as
random
amplification
polymorphic
(RAPD)
directed
minisatellite-region
(DAMD)
employed
to
assess
cultivated
produced
monomorphic
banding
pattern
confirming
genetic
stability.
Additionally,
no
changes
flow
cytometric
profile
ploidy
between
donor
plants
detected.
Regeneration
valuable
will
open
up
new
avenues
pharmaceutical
biotechnology
by
providing
unconventional
steadfast
system
mass
multiplication
might
be
used
manipulation
enhanced
bioactive
constituents.
In Vitro Cellular & Developmental Biology - Plant,
Journal Year:
2022,
Volume and Issue:
58(6), P. 837 - 852
Published: Nov. 21, 2022
Abstract
In
preparation
for
a
major
GWAS
(Genome
Wide
Association
Study)
of
plant
regeneration
and
transformation,
large
number
factors
were
examined
their
effects
on
indirect
rate
in
diverse
wild
genotypes—seeking
high
regeneration,
but
also
highly
genetically
variable
heritable
treatments.
Many
the
have
never
before
been
reported
callus,
shoot,
or
root
organogenesis
poplar
(
Populus
).
Stems
had
highest
potential,
followed
by
petioles
leaves,
with
greenhouse
grown
explant
sources
superior
to
vitro
growth
sources.
Changes
±
50%
Murashige
Skoog
(MS)
basal
medium
salts
micronutrients
minor
effect
regeneration.
popular
treatments
that
evaluated
little
no
useful
at
levels
studied,
including
activated
charcoal,
ascorbic
acid,
silver
nitrate,
melatonin,
serotonin,
sucrose
concentration,
lipoic
acid.
As
result
this
wide
exploration,
treatment
combinations
substantially
elevated
genotypes
identified,
enabling
GWAS.
